FastQCFastQC Report
Sat 18 Jun 2016
SRR1931002_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931002_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295448
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1045974.55671398350126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.054.524961
GTATCAA34650.045.215921
ATCAACG43100.036.3210533
TCAACGC45700.035.1339764
TATCAAC45500.034.6536142
CAACGCA46200.034.091235
AACGCAG50100.031.910346
ACGCAGA55250.027.8354647
CGCAGAG57200.026.9820638
GTACATG42000.026.2956541
GTGGTAT8250.026.151111
TACATGG43900.024.5683692
GCAGAGT61750.023.147899
ACATGGG44250.022.4815853
GGCGTCG3550.022.4228048
GAGTACT36650.021.30231712-13
GTATAAG7500.021.232211
TACGGGC3401.8189894E-1120.7905274
AGAGTAC53700.020.51686510-11
CAGAGTA58050.020.357610-11
Produced by FastQC (version 0.11.5)