FastQCFastQC Report
Sat 18 Jun 2016
SRR1931002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295448
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47800.20823821755056093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45480.19813125803764667No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA32620.14210733591002717No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA32460.14141030421948134No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC29980.13060631301602127No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC25640.11169932840996614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24550.10695080001812284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.055.3579751
GTATCAA55650.040.6522181
CAACGCA70300.031.7829115
ATCAACG71150.031.7231183
TATCAAC72600.031.0281412
TCAACGC72450.031.0227244
AACGCAG75200.030.1522166
GTGGTAT11450.028.6408621
ACGCAGA83250.026.4388717
CGCAGAG83900.026.0644258
GTACATG62050.023.7444651
TACATGG67250.021.9070282
GCAGAGT99750.021.637539
ACATGGG65950.021.1110573
GAGTACT59350.020.70085312-13
GGGCTAT19100.020.6136046
TGGTATC16500.020.4497722
AGAGTAC88600.019.91674610-11
CAGAGTA95500.019.57050510-11
GAGTACA49350.019.45391
Produced by FastQC (version 0.11.5)