##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931002_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2295448 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35757333644674 31.0 31.0 34.0 30.0 34.0 2 31.52399008820936 31.0 31.0 34.0 30.0 34.0 3 31.58784429009065 31.0 31.0 34.0 30.0 34.0 4 35.355573291139684 37.0 35.0 37.0 33.0 37.0 5 35.22660543824125 37.0 35.0 37.0 33.0 37.0 6 35.24739397276697 37.0 35.0 37.0 32.0 37.0 7 35.12539164468112 37.0 35.0 37.0 32.0 37.0 8 35.13296576528852 37.0 35.0 37.0 32.0 37.0 9 36.63353863820919 39.0 35.0 39.0 32.0 39.0 10-11 36.574319697070024 38.5 35.0 39.0 32.0 39.0 12-13 36.457547284887305 38.0 35.0 39.0 32.0 39.0 14-15 37.529732758049846 39.0 36.0 41.0 32.0 41.0 16-17 37.480748202529526 39.0 36.0 41.0 32.0 41.0 18-19 37.49528305585663 39.0 36.0 41.0 32.0 41.0 20-21 37.467751175369685 39.0 36.0 41.0 32.0 41.0 22-23 37.37491570272992 39.0 36.0 41.0 32.0 41.0 24-25 37.251361607843 39.0 36.0 40.5 31.0 41.0 26-27 36.99145439147391 39.0 36.0 40.0 31.0 41.0 28-29 36.81489757119307 39.0 36.0 40.0 30.0 41.0 30-31 36.60822593236701 38.5 35.0 40.0 30.0 41.0 32-33 36.459510082563405 38.0 35.0 40.0 30.0 41.0 34-35 36.54612215131861 38.0 35.0 40.0 30.0 41.0 36-37 36.63102322509593 39.0 35.0 40.0 30.0 41.0 38-39 36.621376306498775 39.0 35.0 40.0 30.0 41.0 40-41 36.51126969550171 39.0 35.0 40.0 30.0 41.0 42-43 36.381345384430404 39.0 35.0 40.0 30.0 41.0 44-45 36.21640111211406 38.5 35.0 40.0 30.0 41.0 46-47 35.98523185887896 38.0 35.0 40.0 29.0 41.0 48-49 35.77206039953857 38.0 35.0 40.0 28.0 41.0 50-51 35.50440262641541 38.0 34.0 40.0 27.5 41.0 52-53 35.293205944983285 38.0 34.0 40.0 27.0 41.0 54-55 35.14037521215902 38.0 34.0 40.0 27.0 41.0 56-57 34.961604880615894 38.0 34.0 40.0 26.0 41.0 58-59 34.75119562717169 38.0 33.5 40.0 26.0 41.0 60-61 34.43194204355751 37.0 33.0 40.0 26.0 41.0 62-63 34.162417750260516 37.0 33.0 40.0 26.0 41.0 64-65 33.83705729774754 36.5 32.5 39.5 25.0 41.0 66-67 33.51603216452736 36.0 32.0 39.0 24.5 41.0 68-69 33.18633530360958 36.0 32.0 39.0 23.5 40.0 70-71 32.82410143902192 35.0 31.5 38.5 23.0 40.0 72-73 32.41428143874312 35.0 31.0 38.0 22.0 40.0 74-75 31.899144742115702 35.0 31.0 37.0 21.0 39.5 76-77 30.502884839909246 33.5 29.5 35.5 20.0 38.5 78-79 31.261162744701686 34.0 30.0 36.0 20.5 39.0 80-81 31.245646383625335 34.0 31.0 36.0 21.0 38.5 82-83 30.99374152670851 34.0 31.0 35.5 20.0 37.0 84-85 30.66910228417285 34.0 30.0 35.0 20.0 37.0 86-87 30.3385814011034 34.0 30.0 35.0 18.5 36.5 88-89 29.998928749420593 34.0 30.0 35.0 18.0 36.0 90-91 29.67658491937086 34.0 29.5 35.0 14.0 36.0 92-93 29.367907484726295 34.0 29.0 35.0 7.5 35.0 94-95 29.00324838550035 34.0 29.0 35.0 2.0 35.0 96-97 28.593344959241072 34.0 29.0 35.0 2.0 35.0 98-99 28.11705340308297 34.0 29.0 35.0 2.0 35.0 100-101 26.998179440353255 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3237605010243243 1101 2 0.42611636122095575 1101 3 0.38189918261196 1101 4 0.22503400331464007 1101 5 0.29825155318433616 1101 6 0.38061331125297215 1101 7 0.37001184919938623 1101 8 0.3527724312602345 1101 9 0.6323849907177177 1101 10-11 0.4858830304056667 1101 12-13 0.48382309204051666 1101 14-15 0.6766004276559627 1101 16-17 0.7240618543346855 1101 18-19 0.6672343585565059 1101 20-21 0.6389613678423629 1101 22-23 0.6987700948873794 1101 24-25 0.5307718067620115 1101 26-27 0.4400129787509073 1101 28-29 0.4991753294420107 1101 30-31 0.6177239991335668 1101 32-33 0.5551835777864582 1101 34-35 0.6169166134267954 1101 36-37 0.5050721980746715 1101 38-39 0.7458486699953042 1101 40-41 0.7859550011108922 1101 42-43 0.7628277397480474 1101 44-45 0.7752246360325685 1101 46-47 0.7170028966406576 1101 48-49 0.7155880176158078 1101 50-51 0.82419608418639 1101 52-53 0.7849773034933918 1101 54-55 0.8531702369034875 1101 56-57 0.8901578504335674 1101 58-59 0.8079395884719744 1101 60-61 0.7806352682162583 1101 62-63 0.5100134905362168 1101 64-65 0.5772601607996535 1101 66-67 0.6961581662738539 1101 68-69 0.5321212192329909 1101 70-71 0.5535720549907879 1101 72-73 0.5488698555934377 1101 74-75 0.6384502688352462 1101 76-77 0.6797548175514727 1101 78-79 0.8377017377431919 1101 80-81 0.5699036581324997 1101 82-83 0.7235947056899938 1101 84-85 0.6542543386153525 1101 86-87 0.6414307688299878 1101 88-89 0.739537895481714 1101 90-91 0.6053051926740238 1101 92-93 0.6506046613228946 1101 94-95 0.8029951818260912 1101 96-97 0.9894937068139065 1101 98-99 0.9231038942022138 1101 100-101 1.2033186898246164 1102 1 0.2918939940837397 1102 2 0.42662918962245655 1102 3 0.41539551089904947 1102 4 0.07354003246145169 1102 5 0.2743174459614721 1102 6 0.3043132133790891 1102 7 0.37464749490745675 1102 8 0.3933981703619054 1102 9 0.5086500924277217 1102 10-11 0.4214384599066676 1102 12-13 0.4307545822425496 1102 14-15 0.5792282165329468 1102 16-17 0.5368979473014193 1102 18-19 0.5304322387888689 1102 20-21 0.5449510958914061 1102 22-23 0.4942505902946124 1102 24-25 0.6332664371582979 1102 26-27 0.49703903551007755 1102 28-29 0.5171224006284376 1102 30-31 0.44880386153327123 1102 32-33 0.48790749074215967 1102 34-35 0.6221334454279059 1102 36-37 0.6658735140842609 1102 38-39 0.7000584008517308 1102 40-41 0.7472774138464189 1102 42-43 0.8896798924928362 1102 44-45 0.7875957799648674 1102 46-47 0.7318794390196572 1102 48-49 0.722163450693337 1102 50-51 0.5327825468241372 1102 52-53 0.4085370286773724 1102 54-55 0.800698296327738 1102 56-57 0.7987298915752064 1102 58-59 0.8876540236204278 1102 60-61 0.700548854611597 1102 62-63 0.4414008192328751 1102 64-65 0.5103852797769974 1102 66-67 0.46376075598997346 1102 68-69 0.577075233889083 1102 70-71 0.6443104740487016 1102 72-73 0.5371165285542858 1102 74-75 0.6237250560220353 1102 76-77 0.7510315237586802 1102 78-79 0.7726916523102467 1102 80-81 0.7855183888254835 1102 82-83 0.6674831640705179 1102 84-85 0.6903260576769163 1102 86-87 0.6748200896159737 1102 88-89 0.6963777226545034 1102 90-91 0.6382603302839414 1102 92-93 0.5937294534129904 1102 94-95 0.6499671483605276 1102 96-97 0.7468236725676611 1102 98-99 0.9385447977954726 1102 100-101 1.090871208106261 1103 1 0.31985774929435706 1103 2 0.3854848923279235 1103 3 0.33875256041152824 1103 4 0.29198042824066306 1103 5 0.32288624257253673 1103 6 0.35992124820911187 1103 7 0.3339457374703372 1103 8 0.27506138675520475 1103 9 0.3893486437665956 1103 10-11 0.3634829411170628 1103 12-13 0.37106312004814157 1103 14-15 0.35803106776753424 1103 16-17 0.4070537096108211 1103 18-19 0.5560002106235942 1103 20-21 0.460565777326039 1103 22-23 0.3564633198278955 1103 24-25 0.3668231324498521 1103 26-27 0.3776735026918985 1103 28-29 0.4098056345305068 1103 30-31 0.35771976364821967 1103 32-33 0.5856121058789867 1103 34-35 0.5276307696780549 1103 36-37 0.48695338317109815 1103 38-39 0.4637988286352197 1103 40-41 0.4183292768333615 1103 42-43 0.44370614506873096 1103 44-45 0.5041703626758647 1103 46-47 0.5100838878968617 1103 48-49 0.3507322435236162 1103 50-51 0.34701552468258967 1103 52-53 0.40552913763026766 1103 54-55 0.40991887229496626 1103 56-57 0.48828326658907883 1103 58-59 0.4317492167604371 1103 60-61 0.2622776825955455 1103 62-63 0.28592197352654836 1103 64-65 0.3203317080682453 1103 66-67 0.14384782348572145 1103 68-69 0.03474235506806167 1103 70-71 0.04722808015949198 1103 72-73 0.163418556997442 1103 74-75 0.23053954599815896 1103 76-77 0.37599525193674666 1103 78-79 0.41106711703853804 1103 80-81 0.20237618948367597 1103 82-83 0.2437537080996428 1103 84-85 0.35770196645736974 1103 86-87 0.2886456510281654 1103 88-89 0.23323093345036128 1103 90-91 0.3862866233141382 1103 92-93 0.24496125866369667 1103 94-95 0.28424037636269617 1103 96-97 0.3988891389765037 1103 98-99 0.3827262682489945 1103 100-101 0.5743807854740837 1104 1 0.35441620882726355 1104 2 0.4251835312436363 1104 3 0.37197208816637684 1104 4 0.1721014754983301 1104 5 0.34659160223022667 1104 6 0.40245660392353955 1104 7 0.42279016435699646 1104 8 0.39493255079807454 1104 9 0.4682043352862806 1104 10-11 0.3373748582402314 1104 12-13 0.43475665899588734 1104 14-15 0.5563549034158299 1104 16-17 0.5932659364561488 1104 18-19 0.6291042192860843 1104 20-21 0.5426840282594583 1104 22-23 0.5110013512322951 1104 24-25 0.5057800517040434 1104 26-27 0.5571138176161909 1104 28-29 0.6492047164420853 1104 30-31 0.6372113571962004 1104 32-33 0.6611031697954104 1104 34-35 0.7532346157740761 1104 36-37 0.7183699537922337 1104 38-39 0.6472832899080885 1104 40-41 0.6221292927367941 1104 42-43 0.6005089672586976 1104 44-45 0.8174547684859519 1104 46-47 0.6713842311973721 1104 48-49 0.5668015441110796 1104 50-51 0.547871412187682 1104 52-53 0.5287210046340576 1104 54-55 0.5314021159948581 1104 56-57 0.673883808827469 1104 58-59 0.6176270729263393 1104 60-61 0.5152982942113127 1104 62-63 0.5721674563072696 1104 64-65 0.5760860626304876 1104 66-67 0.5438784167726922 1104 68-69 0.44215582035283063 1104 70-71 0.5575888617754075 1104 72-73 0.4594442010187407 1104 74-75 0.5084888864854982 1104 76-77 0.5535113052614449 1104 78-79 0.7008689562221839 1104 80-81 0.5389099537675612 1104 82-83 0.5327851905662833 1104 84-85 0.5946454344464485 1104 86-87 0.6424143581710702 1104 88-89 0.6242648543233322 1104 90-91 0.6311566326370297 1104 92-93 0.6665586702071664 1104 94-95 0.8113289297095818 1104 96-97 0.7787470006452146 1104 98-99 0.8652956116743056 1104 100-101 1.2556642146174788 1105 1 0.3101667321425623 1105 2 0.33534695580644325 1105 3 0.36103174767507085 1105 4 0.14723474228952682 1105 5 0.16560116740136266 1105 6 0.26551852252728025 1105 7 0.31090081620917687 1105 8 0.3218973429164791 1105 9 0.42354548888492616 1105 10-11 0.4200479851260681 1105 12-13 0.43693883425618907 1105 14-15 0.47048191336899237 1105 16-17 0.484328222188914 1105 18-19 0.5136152858271714 1105 20-21 0.47919521787220276 1105 22-23 0.4674462385956559 1105 24-25 0.4865840016785441 1105 26-27 0.39747944474451913 1105 28-29 0.4504059167036658 1105 30-31 0.3965614136735809 1105 32-33 0.5388333007624269 1105 34-35 0.5690192862601293 1105 36-37 0.5259096052908419 1105 38-39 0.5785754067510638 1105 40-41 0.5745785230695759 1105 42-43 0.6617626711420286 1105 44-45 0.6028292661606187 1105 46-47 0.7133151629645624 1105 48-49 0.6755442210865681 1105 50-51 0.5534450982151995 1105 52-53 0.6527136161625293 1105 54-55 0.6889380127648579 1105 56-57 0.7288595543236198 1105 58-59 0.8315631653594124 1105 60-61 0.7867607401458301 1105 62-63 0.44946918316954054 1105 64-65 0.5266900002978261 1105 66-67 0.4665643827444228 1105 68-69 0.20752778358569657 1105 70-71 0.3084193347356248 1105 72-73 0.4073277856581399 1105 74-75 0.6570442025032754 1105 76-77 0.7134228501132434 1105 78-79 0.7670236239234818 1105 80-81 0.5315545968649644 1105 82-83 0.5866551163373828 1105 84-85 0.7097719322963485 1105 86-87 0.568573448108296 1105 88-89 0.7895779934746159 1105 90-91 0.6669770043343028 1105 92-93 0.7494359604612484 1105 94-95 0.7034847801503972 1105 96-97 0.6250148525545463 1105 98-99 0.5965000660340181 1105 100-101 0.6295757344377506 1106 1 0.1886446481878501 1106 2 0.16940841093619596 1106 3 0.1876129373807096 1106 4 0.2134195697164074 1106 5 0.14302114104046382 1106 6 0.13237742984954792 1106 7 0.22933673633441742 1106 8 0.27173119501080834 1106 9 0.3922702856605653 1106 10-11 0.2784587200752924 1106 12-13 0.2747692994497015 1106 14-15 0.2964298640052263 1106 16-17 0.256629272705716 1106 18-19 0.2991375224193149 1106 20-21 0.2008902565369155 1106 22-23 0.2823295225280873 1106 24-25 0.32954586913562167 1106 26-27 0.3631334445671328 1106 28-29 0.407369380665763 1106 30-31 0.35916885289191214 1106 32-33 0.5488093944405605 1106 34-35 0.5133257038390155 1106 36-37 0.5165059019767568 1106 38-39 0.5563568629534075 1106 40-41 0.43253753373150516 1106 42-43 0.4690687442553525 1106 44-45 0.6707632360332951 1106 46-47 0.6232843467049705 1106 48-49 0.5735189905318521 1106 50-51 0.4731975161777271 1106 52-53 0.2996420972757079 1106 54-55 0.5006148793246794 1106 56-57 0.590297770198319 1106 58-59 0.5473270022518832 1106 60-61 0.5839705691712851 1106 62-63 0.3731262316154229 1106 64-65 0.34530671327138407 1106 66-67 0.4086709649242053 1106 68-69 0.27270706037304393 1106 70-71 0.5113095451071317 1106 72-73 0.5135361068733033 1106 74-75 0.7998715840081623 1106 76-77 0.7047292202507585 1106 78-79 0.5661305918221835 1106 80-81 0.558118150759384 1106 82-83 0.6201714083204095 1106 84-85 0.6108943330768639 1106 86-87 0.6054457008923819 1106 88-89 0.6291606951158819 1106 90-91 0.5799700593312167 1106 92-93 0.5968005927184876 1106 94-95 0.6613903677116859 1106 96-97 0.7011278166290573 1106 98-99 0.7420120099129477 1106 100-101 0.7699057406876371 1107 1 0.16766525725250148 1107 2 0.19301060725592833 1107 3 0.1887691896380197 1107 4 0.10237865781845557 1107 5 0.21617686019070703 1107 6 0.1391053631338437 1107 7 0.16192158327470452 1107 8 0.2130085358239242 1107 9 0.19447026795815248 1107 10-11 0.279366222946841 1107 12-13 0.23574108038339148 1107 14-15 0.30807555738523007 1107 16-17 0.3113744851702407 1107 18-19 0.4099683453677372 1107 20-21 0.29327318408827807 1107 22-23 0.29306796738151775 1107 24-25 0.16474100768750333 1107 26-27 0.31773006869714493 1107 28-29 0.36495226914973244 1107 30-31 0.23081035466673683 1107 32-33 0.23044299117354683 1107 34-35 0.24421015288201886 1107 36-37 0.21952177208582668 1107 38-39 0.24088897502391404 1107 40-41 0.17456763393325048 1107 42-43 0.24532426735649437 1107 44-45 0.20747335499552833 1107 46-47 0.20428075840604976 1107 48-49 0.18546969440613736 1107 50-51 0.13873361942957985 1107 52-53 0.22943601680299963 1107 54-55 0.1964699210016434 1107 56-57 0.0644001104773082 1107 58-59 0.12362820571057398 1107 60-61 0.22958433920477006 1107 62-63 0.19874451881566557 1107 64-65 0.08789001379421535 1107 66-67 0.11105504211484885 1107 68-69 0.07997944451128092 1107 70-71 0.04463026292969374 1107 72-73 0.16823685983617054 1107 74-75 0.19301413826248748 1107 76-77 0.24030546931350116 1107 78-79 0.362877163506262 1107 80-81 0.2984711001945861 1107 82-83 0.3412129611677024 1107 84-85 0.25031658337876905 1107 86-87 0.23264972727507782 1107 88-89 0.2443464127298789 1107 90-91 0.3698561839989196 1107 92-93 0.29517237792516937 1107 94-95 0.4920121292642854 1107 96-97 0.5273265190627328 1107 98-99 0.6496290823615851 1107 100-101 0.7584192672621093 1108 1 0.15536982575711633 1108 2 0.15594988928462783 1108 3 0.06814269935186701 1108 4 0.03592263976420185 1108 5 0.07009174588635858 1108 6 0.1460749301731994 1108 7 0.013867041838402372 1108 8 0.19475623233643802 1108 9 0.18769338776597522 1108 10-11 0.19956111145069855 1108 12-13 0.1315423166957146 1108 14-15 0.23958108004160295 1108 16-17 0.22174075270704918 1108 18-19 0.1532285364494257 1108 20-21 0.19534093177842493 1108 22-23 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-1.4834660422769907 2316 82-83 -1.2037644060694817 2316 84-85 -1.2270025758218814 2316 86-87 -1.2134609391662643 2316 88-89 -1.1951560069546083 2316 90-91 -1.0816016622353075 2316 92-93 -0.964483610683029 2316 94-95 -1.4632189102126887 2316 96-97 -1.3194466743863629 2316 98-99 -1.3037793269112328 2316 100-101 -2.059516833588585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 33.0 5 58.0 6 111.0 7 324.0 8 726.0 9 1406.0 10 2359.0 11 3725.0 12 5299.0 13 6970.0 14 8568.0 15 11160.0 16 13551.0 17 15838.0 18 13974.0 19 11228.0 20 10638.0 21 11304.0 22 12632.0 23 15036.0 24 18639.0 25 23569.0 26 30499.0 27 39605.0 28 50907.0 29 63581.0 30 77727.0 31 94388.0 32 113756.0 33 139116.0 34 173082.0 35 217217.0 36 285832.0 37 388961.0 38 360157.0 39 73451.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.99580016084861 22.185606750413665 14.83924287878933 22.97935020994839 2 14.980712575992975 21.67171035491126 40.68583413425302 22.661742934842753 3 18.21954188336837 26.726258365651624 31.141353264226947 23.912846486753057 4 12.519693630392565 17.923363955746613 39.30215811639427 30.254784297466557 5 12.300339810054894 38.66994859283785 36.116842380413 12.91286921669426 6 29.301522977977612 39.629055382482726 17.811161883336375 13.258259756203286 7 25.57030261630845 33.517335178143874 23.469449101003377 17.442913104544296 8 24.274549900520032 37.50070248061698 20.51927367207203 17.70547394679096 9 24.815330166486017 16.551148185452252 21.709618340297844 36.92390330776389 10-11 23.85405811850236 27.492236809546544 29.02960554976632 19.624099522184775 12-13 24.241781996368463 24.876930342138007 29.565317968431437 21.315969693062094 14-15 21.569166454652862 26.63342842007312 26.808557632322756 24.988847492951265 16-17 20.89071501510816 29.857417811250787 27.745629611300277 21.50623756234077 18-19 20.798096929226887 28.645475741554588 29.956134924424337 20.600292404794185 20-21 21.36487082260195 27.801326799822956 29.854019781759376 20.979782595815717 22-23 21.378929529850485 27.621327501481197 29.667427595580804 21.332315373087514 24-25 21.14229602782399 28.031311787184055 29.73267094458793 21.09372124040403 26-27 21.14806277503724 27.96456433404447 29.668711506767316 21.218661384150973 28-29 20.985483620471268 28.27172898982614 29.564312573419972 21.17847481628262 30-31 21.39937964225412 27.904226418596178 29.552058488508088 21.14433545064161 32-33 21.33941943138804 28.06956731651181 29.344689246900924 21.246324005199227 34-35 21.480682197941626 27.78035572135727 29.784398227362423 20.954563853338684 36-37 21.42453699639652 27.869437372994348 29.81858000247885 20.887445628130283 38-39 21.45479424841795 27.85420473129747 29.737440087686966 20.95356093259761 40-41 21.29528722890506 27.92683859238766 29.65040153298535 21.127472645721937 42-43 21.266899619636018 28.042911854023885 29.3894787749927 21.300709751347394 44-45 21.134061267026365 28.361900853235184 29.307892251174593 21.19614562856386 46-47 21.46844839706786 28.05288638015811 29.122103255751338 21.356561967022696 48-49 21.58135102952575 28.12618863071049 28.887914384168933 21.404545955594827 50-51 21.506346046524214 28.43504198869054 28.508222371459084 21.55038959332616 52-53 21.357706882353764 28.575952238056026 28.50913157564169 21.557209303948525 54-55 21.289122794939594 28.579125315599967 28.58156536712863 21.55018652233181 56-57 21.365975647877768 28.520301967187045 28.541737237137475 21.57198514779771 58-59 21.416494028261646 28.479835783458824 28.719942780417675 21.383727407861855 60-61 21.38622525026183 28.59837670594404 28.68197543379517 21.33342260999896 62-63 21.3054457188127 28.46656115351727 28.504905750029913 21.72308737764012 64-65 21.067388004032082 28.412354627547785 28.520122849221448 22.000134519198685 66-67 21.10783827894504 28.06450549088151 28.783409878585374 22.04424635158808 68-69 21.10249282434266 28.339429611949974 28.59361047105463 21.964467092652733 70-71 21.1557017883849 28.075749731363413 28.74973769583285 22.01881078441884 72-73 21.31244617531938 28.331762730880193 28.70213980856469 21.653651285235735 74-75 21.30711107849202 28.516134218242694 28.49160437002632 21.68515033323897 76-77 21.137149518428934 28.427602797740153 28.549065236943445 21.88618244688747 78-79 20.889401528989033 28.49816418932975 28.71606527665683 21.896369005024383 80-81 21.067104170605624 28.308572713102375 28.70150288876323 21.922820227528767 82-83 21.04327782637638 28.302427238604405 28.87887244668579 21.775422488333433 84-85 21.32860339245324 28.261476626784837 28.47097821427451 21.938941766487414 86-87 20.992525206408512 28.420399852229284 28.503825832691483 22.083249108670724 88-89 21.11497359226067 28.233442368133925 28.398247049754417 22.253336989850986 90-91 21.071308139306915 27.92795383273516 28.799907289049916 22.200830738908014 92-93 21.06420233081565 28.20494777091489 28.47583706294483 22.255012835324624 94-95 20.56870881664216 28.152916190165005 28.673092631922888 22.605282361269946 96-97 20.640881710621066 28.03011788117957 28.666355616139256 22.662644792060107 98-99 20.618088495143432 28.203274480624263 28.543164558726662 22.635472465505643 100-101 20.60639143208646 28.05543841550756 28.471609899244072 22.86656025316191 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 157.0 1 122.5 2 105.5 3 148.0 4 154.0 5 164.5 6 275.5 7 530.0 8 898.5 9 1191.0 10 1165.0 11 1058.0 12 1166.0 13 1329.0 14 1507.0 15 1732.0 16 1982.5 17 2251.5 18 2528.5 19 2804.5 20 3078.5 21 3426.5 22 4005.0 23 4846.0 24 6069.0 25 7745.0 26 10388.0 27 14047.0 28 18700.5 29 26309.0 30 35829.0 31 44729.5 32 53119.5 33 60187.0 34 66179.0 35 70866.0 36 75918.5 37 82998.0 38 85090.0 39 80684.0 40 77420.5 41 76128.0 42 76929.0 43 80464.0 44 84662.5 45 90540.0 46 93453.5 47 94045.0 48 96067.0 49 96543.0 50 94534.5 51 89858.5 52 82393.0 53 74810.0 54 68387.5 55 59410.0 56 47927.0 57 37124.0 58 28818.0 59 22317.5 60 16203.5 61 11367.0 62 8080.0 63 5405.5 64 3158.0 65 1722.0 66 972.5 67 525.0 68 277.5 69 152.0 70 105.0 71 79.5 72 52.5 73 31.5 74 21.5 75 16.0 76 11.5 77 7.5 78 6.0 79 5.5 80 3.5 81 2.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005314866640411807 2 0.007405961712049238 3 0.002178224032955658 4 0.17852724174104576 5 0.002091095071637432 6 4.792092872502449E-4 7 0.0 8 4.792092872502449E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.485158452729053E-4 24-25 8.059428921935937E-4 26-27 0.003354465010751714 28-29 2.8316912428423557E-4 30-31 0.0069485346651285505 32-33 0.021390160003624564 34-35 0.014681229982121137 36-37 0.0012415876987847251 38-39 4.356448065911317E-4 40-41 0.002504957637899007 42-43 0.012110925623233461 44-45 0.008015864441276822 46-47 0.01147924065367632 48-49 0.011544587374664989 50-51 0.19749957306808955 52-53 0.11954093492860653 54-55 0.018057477233202408 56-57 0.00720992154908323 58-59 0.018710944443089104 60-61 0.12847165346372472 62-63 0.24770763702771748 64-65 0.2531967615907657 66-67 0.2640660995152145 68-69 0.2586205394328253 70-71 0.2458997110803643 72-73 0.16835493550714284 74-75 0.27881267621832423 76-77 0.219042208754021 78-79 0.0061208095326054 80-81 8.712896131822633E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.5247568230689607E-4 90-91 0.006186156253594069 92-93 0.018057477233202408 94-95 0.0031802070881152607 96-97 4.356448065911316E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2295448.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.52026701663208 #Duplication Level Percentage of deduplicated Percentage of total 1 66.47942670282953 28.932024012197616 2 16.78099748274324 14.606269825088331 3 6.413238512707296 8.3731755754311 4 3.255293043474906 5.666848898776512 5 1.8600512628233885 4.047496381134878 6 1.1510103635830593 3.005536701722731 7 0.7919695703731248 2.4126709020180046 8 0.5494450186332387 1.9129595137501547 9 0.3962342962327258 1.551980013587597 >10 2.10566308239322 16.097648937114904 >50 0.1332596184929052 3.992935089436235 >100 0.07688254379241324 6.17833416045985 >500 0.0043188551168881935 1.25866111039126 >1k 0.0022096468039892567 1.963458878890918 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4780 0.20823821755056093 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4548 0.19813125803764667 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3262 0.14210733591002717 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3246 0.14141030421948134 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 2998 0.13060631301602127 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 2564 0.11169932840996614 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2455 0.10695080001812284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.356448065911316E-5 0.0 6 0.0 0.0 0.0 4.356448065911316E-5 0.0 7 0.0 0.0 0.0 4.356448065911316E-5 0.0 8 0.0 0.0 0.0 4.356448065911316E-5 0.0 9 0.0 0.0 0.0 4.356448065911316E-5 0.0 10-11 0.0 0.0 0.0 4.356448065911316E-5 0.0 12-13 0.0 0.0 0.0 4.356448065911316E-5 0.0 14-15 0.0 0.0 0.0 4.356448065911316E-5 0.0 16-17 0.0 0.0 0.0 4.356448065911316E-5 0.0 18-19 0.0 0.0 0.0 6.534672098866975E-5 0.0 20-21 0.0 0.0 0.0 1.306934419773395E-4 0.0 22-23 0.0 0.0 0.0 1.9604016296600926E-4 0.0 24-25 0.0 0.0 0.0 3.702980856024619E-4 0.0 26-27 0.0 0.0 0.0 4.5742704692068825E-4 0.0 28-29 0.0 0.0 0.0 0.001306934419773395 0.0 30-31 0.0 0.0 0.0 0.004443577027229543 0.0 32-33 0.0 0.0 0.0 0.013461424523665968 0.0 34-35 0.0 0.0 0.0 0.025790172550194994 0.0 36-37 0.0 0.0 0.0 0.04759419512008113 0.0 38-39 0.0 0.0 0.0 0.08264181981033768 0.0 40-41 4.356448065911316E-5 0.0 0.0 0.1242241165994612 0.0 42-43 4.356448065911316E-5 4.356448065911316E-5 0.0 0.16704800108736945 0.0 44-45 4.356448065911316E-5 4.356448065911316E-5 0.0 0.21945607132028258 0.0 46-47 4.356448065911316E-5 4.356448065911316E-5 0.0 0.2856958641624642 4.356448065911316E-5 48-49 4.356448065911316E-5 4.356448065911316E-5 0.0 0.35940696543768363 4.356448065911316E-5 50-51 4.356448065911316E-5 4.356448065911316E-5 0.0 0.44174383388340754 4.356448065911316E-5 52-53 4.356448065911316E-5 4.356448065911316E-5 0.0 0.5275005140608717 4.356448065911316E-5 54-55 4.356448065911316E-5 6.534672098866975E-5 0.0 0.6163284901248035 4.356448065911316E-5 56-57 4.356448065911316E-5 8.712896131822633E-5 0.0 0.7281585119767471 4.356448065911316E-5 58-59 4.356448065911316E-5 8.712896131822633E-5 0.0 0.8602460173351781 4.356448065911316E-5 60-61 4.356448065911316E-5 8.712896131822633E-5 0.0 1.006448414427162 4.356448065911316E-5 62-63 4.356448065911316E-5 8.712896131822633E-5 0.0 1.1535656656129871 4.356448065911316E-5 64-65 4.356448065911316E-5 8.712896131822633E-5 0.0 1.309199772767669 4.356448065911316E-5 66-67 4.356448065911316E-5 8.712896131822633E-5 0.0 1.4929983166684675 4.356448065911316E-5 68-69 4.356448065911316E-5 8.712896131822633E-5 0.0 1.699145439147391 4.356448065911316E-5 70-71 4.356448065911316E-5 8.712896131822633E-5 0.0 1.9349381907148409 4.356448065911316E-5 72-73 4.356448065911316E-5 8.712896131822633E-5 0.0 2.1821448362149787 4.356448065911316E-5 74-75 4.356448065911316E-5 8.712896131822633E-5 0.0 2.4258445410220575 4.356448065911316E-5 76-77 4.356448065911316E-5 8.712896131822633E-5 0.0 2.7032413716189607 4.356448065911316E-5 78-79 4.356448065911316E-5 1.306934419773395E-4 0.0 3.0196719768864293 4.356448065911316E-5 80-81 4.356448065911316E-5 1.306934419773395E-4 0.0 3.36936406313713 4.356448065911316E-5 82-83 4.356448065911316E-5 1.306934419773395E-4 0.0 3.7407730429964 4.356448065911316E-5 84-85 4.356448065911316E-5 1.306934419773395E-4 0.0 4.1079344859914055 4.356448065911316E-5 86-87 4.356448065911316E-5 1.306934419773395E-4 0.0 4.4949831144072965 4.356448065911316E-5 88-89 4.356448065911316E-5 1.306934419773395E-4 0.0 4.928275439042837 4.356448065911316E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3065 0.0 55.357975 1 GTATCAA 5565 0.0 40.652218 1 CAACGCA 7030 0.0 31.782911 5 ATCAACG 7115 0.0 31.723118 3 TATCAAC 7260 0.0 31.028141 2 TCAACGC 7245 0.0 31.022724 4 AACGCAG 7520 0.0 30.152216 6 GTGGTAT 1145 0.0 28.640862 1 ACGCAGA 8325 0.0 26.438871 7 CGCAGAG 8390 0.0 26.064425 8 GTACATG 6205 0.0 23.744465 1 TACATGG 6725 0.0 21.907028 2 GCAGAGT 9975 0.0 21.63753 9 ACATGGG 6595 0.0 21.111057 3 GAGTACT 5935 0.0 20.700853 12-13 GGGCTAT 1910 0.0 20.613604 6 TGGTATC 1650 0.0 20.449772 2 AGAGTAC 8860 0.0 19.916746 10-11 CAGAGTA 9550 0.0 19.570505 10-11 GAGTACA 4935 0.0 19.4539 1 >>END_MODULE