FastQCFastQC Report
Sat 18 Jun 2016
SRR1931001_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931001_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2498987
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1132204.530635813631684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.053.2084851
GTATCAA36500.042.5515251
ATCAACG41250.036.776333
TCAACGC41450.036.597774
CAACGCA43450.035.0206955
TATCAAC45150.033.375532
AACGCAG47100.033.076396
ACGCAGA52450.029.186537
CGCAGAG54100.028.0849978
GTACATG36500.024.2950951
GCAGAGT58100.024.1175759
ACATGGG36250.023.2185063
TACATGG38450.022.2617232
GAGTACT39250.020.86843312-13
CAGAGTA55750.019.95040310-11
AGAGTAC52250.019.92899510-11
GTGGTAT11250.019.1364821
GAGTACA29400.017.9578881
GTACTTT45300.017.5975414-15
AGTACTT45200.017.43052712-13
Produced by FastQC (version 0.11.5)