##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931001_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2498987 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.858177733617662 28.0 2.0 31.0 2.0 33.0 2 19.578601249226185 27.0 2.0 31.0 2.0 33.0 3 20.21877664829789 27.0 2.0 31.0 2.0 33.0 4 21.724299085989642 30.0 2.0 35.0 2.0 37.0 5 21.069749462482196 30.0 2.0 35.0 2.0 37.0 6 22.025767240886008 30.0 2.0 35.0 2.0 37.0 7 22.200624092882435 30.0 2.0 35.0 2.0 37.0 8 22.622650698062856 30.0 2.0 35.0 2.0 37.0 9 23.898384825531306 32.0 2.0 37.0 2.0 39.0 10-11 24.251637363459672 32.0 2.0 37.0 2.0 39.0 12-13 24.164722545575465 32.0 2.0 37.0 2.0 39.0 14-15 24.12331356665721 32.0 2.0 38.0 2.0 40.0 16-17 22.95942255802051 30.5 2.0 38.0 2.0 40.0 18-19 21.90563056150352 28.0 2.0 37.0 2.0 40.0 20-21 21.571932146905926 27.0 2.0 37.0 2.0 40.0 22-23 21.191909161592278 27.0 2.0 37.0 2.0 39.5 24-25 21.024698607875912 27.0 2.0 37.0 2.0 39.0 26-27 20.75046428812955 26.5 2.0 37.0 2.0 39.0 28-29 20.54780056879047 25.0 2.0 36.0 2.0 39.0 30-31 20.526588373608988 25.0 2.0 36.0 2.0 39.0 32-33 20.40441967085063 25.0 2.0 36.0 2.0 39.0 34-35 20.08529115997802 24.5 2.0 36.0 2.0 39.0 36-37 19.637779428224317 24.0 2.0 36.0 2.0 39.0 38-39 19.520146963549628 24.0 2.0 35.0 2.0 39.0 40-41 19.385931779557076 23.5 2.0 35.0 2.0 39.0 42-43 19.242686736665696 23.5 2.0 35.0 2.0 39.0 44-45 18.86881444361255 21.0 2.0 35.0 2.0 38.0 46-47 18.53509622098874 19.0 2.0 35.0 2.0 38.0 48-49 18.29068938733975 16.5 2.0 34.5 2.0 38.0 50-51 16.073547601488123 10.0 2.0 32.5 2.0 37.5 52-53 17.91858180934915 17.0 2.0 33.5 2.0 38.0 54-55 18.68500596441678 20.0 2.0 35.0 2.0 39.0 56-57 18.655606651815315 19.5 2.0 35.5 2.0 39.0 58-59 18.37567102189807 18.5 2.0 35.0 2.0 39.0 60-61 18.0830450498542 15.0 2.0 35.0 2.0 39.0 62-63 17.640237024042143 11.0 2.0 34.0 2.0 38.0 64-65 17.28831562549145 8.5 2.0 34.0 2.0 38.0 66-67 16.929841571804896 8.0 2.0 34.0 2.0 38.0 68-69 16.49138330851661 7.0 2.0 33.0 2.0 38.0 70-71 16.141681409307054 2.0 2.0 32.5 2.0 37.0 72-73 15.73476792796441 2.0 2.0 32.0 2.0 36.5 74-75 15.280787375044369 2.0 2.0 31.5 2.0 36.0 76-77 14.863096726793696 2.0 2.0 31.0 2.0 35.5 78-79 14.485964112658449 2.0 2.0 31.0 2.0 35.0 80-81 14.047971037864542 2.0 2.0 30.0 2.0 35.0 82-83 13.586220536561415 2.0 2.0 30.0 2.0 34.0 84-85 13.18069781875616 2.0 2.0 29.0 2.0 34.0 86-87 12.694404172570724 2.0 2.0 29.0 2.0 34.0 88-89 12.272787333427505 2.0 2.0 29.0 2.0 34.0 90-91 11.78378238862387 2.0 2.0 27.5 2.0 34.0 92-93 11.244184343495984 2.0 2.0 26.0 2.0 34.0 94-95 10.675323841220463 2.0 2.0 25.5 2.0 33.0 96-97 10.04039836941929 2.0 2.0 24.5 2.0 33.0 98-99 9.382781703146115 2.0 2.0 19.0 2.0 32.0 100-101 8.474959253489514 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.965231041476958 1101 2 9.582767242015773 1101 3 9.05195151586721 1101 4 10.85203648029783 1101 5 11.752986268734375 1101 6 10.876868373143271 1101 7 10.383865330572991 1101 8 9.813585017252382 1101 9 9.89080055434614 1101 10-11 9.373834568031398 1101 12-13 9.309348124478351 1101 14-15 9.98918093828841 1101 16-17 10.968238632785358 1101 18-19 11.709298656691832 1101 20-21 11.766420738134304 1101 22-23 11.969655713074825 1101 24-25 12.094456284275953 1101 26-27 12.015868924735791 1101 28-29 11.832386608652055 1101 30-31 11.672999089842634 1101 32-33 11.538377889709555 1101 34-35 11.258487509647079 1101 36-37 11.733607500025975 1101 38-39 11.574644201815726 1101 40-41 11.32923865440626 1101 42-43 10.990033753494803 1101 44-45 11.156011129958074 1101 46-47 10.785195245410808 1101 48-49 11.037170715733993 1101 50-51 12.315796117335632 1101 52-53 11.52072041394478 1101 54-55 11.426755190331793 1101 56-57 11.50640508629305 1101 58-59 11.281152435666979 1101 60-61 10.925706354233931 1101 62-63 10.994566114025183 1101 64-65 10.946031324133383 1101 66-67 10.647901892510223 1101 68-69 10.493513546308936 1101 70-71 10.373583438114506 1101 72-73 10.291426167059953 1101 74-75 9.763653359635166 1101 76-77 9.645760814371089 1101 78-79 9.284850501357019 1101 80-81 9.107296554636322 1101 82-83 8.607458994786663 1101 84-85 8.3844857383668 1101 86-87 8.19762789636921 1101 88-89 8.00061505640515 1101 90-91 7.570913333194758 1101 92-93 7.27373367830023 1101 94-95 7.072035746783332 1101 96-97 6.873805338224857 1101 98-99 6.46963143524356 1101 100-101 5.920196492587483 1102 1 7.791431482501217 1102 2 9.074854945137318 1102 3 8.50801569514175 1102 4 10.46402609717357 1102 5 11.06853357064449 1102 6 10.333917340623717 1102 7 10.126470801533468 1102 8 9.312437932886144 1102 9 9.215707413585097 1102 10-11 8.414957494685979 1102 12-13 8.610702651104976 1102 14-15 9.22074763722281 1102 16-17 10.07489138698471 1102 18-19 11.103395385203388 1102 20-21 10.940827112399784 1102 22-23 11.013447430129897 1102 24-25 10.890255877721675 1102 26-27 11.150807888932015 1102 28-29 10.923875922479208 1102 30-31 10.740383354487829 1102 32-33 10.810025277124375 1102 34-35 10.524894981206952 1102 36-37 10.635753687215207 1102 38-39 10.783173942228043 1102 40-41 10.390123652017301 1102 42-43 9.999499114515192 1102 44-45 9.78929553506844 1102 46-47 9.819667141365091 1102 48-49 9.670839796256825 1102 50-51 11.403136346580157 1102 52-53 10.548336853061699 1102 54-55 10.12756509125932 1102 56-57 9.946326063861708 1102 58-59 10.123628452698977 1102 60-61 9.854037040081227 1102 62-63 9.941295870331992 1102 64-65 9.712608512474485 1102 66-67 9.591303859405638 1102 68-69 9.275193852555564 1102 70-71 9.011247924554878 1102 72-73 8.917775164127136 1102 74-75 8.612758145504769 1102 76-77 8.495700061029654 1102 78-79 8.04154222578484 1102 80-81 7.641409627663849 1102 82-83 7.366573911000673 1102 84-85 7.483516078652537 1102 86-87 7.200606437848569 1102 88-89 7.181503223339439 1102 90-91 6.960334591789513 1102 92-93 6.557412525317664 1102 94-95 6.166052380464141 1102 96-97 5.625598974108872 1102 98-99 5.085459906873329 1102 100-101 4.545480271493194 1103 1 7.824787909856084 1103 2 9.160286548445907 1103 3 8.50708732776009 1103 4 10.561075958762988 1103 5 11.099746737676298 1103 6 10.421504795410723 1103 7 9.940803160127118 1103 8 9.545794421661284 1103 9 9.2972105764219 1103 10-11 8.712792675848142 1103 12-13 8.703997584485482 1103 14-15 9.249138235744912 1103 16-17 10.263755339243964 1103 18-19 11.029277856288257 1103 20-21 11.424674195581868 1103 22-23 11.516321745217109 1103 24-25 11.31393858183505 1103 26-27 11.403892261342765 1103 28-29 11.181892342574198 1103 30-31 10.974226752453713 1103 32-33 11.172652086949409 1103 34-35 11.103796227854172 1103 36-37 11.227941213126886 1103 38-39 10.947525371815537 1103 40-41 10.611330750726431 1103 42-43 10.343946906364355 1103 44-45 10.497857428581664 1103 46-47 10.32216418244358 1103 48-49 10.063292442764133 1103 50-51 11.651828862581675 1103 52-53 10.977088418510832 1103 54-55 10.518950714125353 1103 56-57 10.274237983509256 1103 58-59 10.20427081045117 1103 60-61 9.86979978463371 1103 62-63 10.043930216424158 1103 64-65 9.640616432004105 1103 66-67 9.357393666896083 1103 68-69 9.166374638821008 1103 70-71 8.770400619645615 1103 72-73 8.725688336748192 1103 74-75 8.476321307346568 1103 76-77 8.109041188595258 1103 78-79 7.793598729976468 1103 80-81 7.537623805059104 1103 82-83 7.25565934475369 1103 84-85 7.119099942810555 1103 86-87 6.6648117793237684 1103 88-89 6.4963628749513855 1103 90-91 6.31526851279199 1103 92-93 6.0724575740147255 1103 94-95 5.7152636827765555 1103 96-97 5.600953937204503 1103 98-99 5.360694885471435 1103 100-101 4.545269139314929 1104 1 7.845800136869869 1104 2 9.324321432901677 1104 3 8.581045563916302 1104 4 10.522238412832692 1104 5 11.356410505329933 1104 6 10.697168433662046 1104 7 10.313095115817358 1104 8 9.551175150346769 1104 9 9.37002525726464 1104 10-11 8.885872330494436 1104 12-13 8.758393350923331 1104 14-15 9.24860497784611 1104 16-17 10.247209297600492 1104 18-19 11.133543618543104 1104 20-21 11.336589440077002 1104 22-23 11.667881043074157 1104 24-25 11.408579520513403 1104 26-27 11.369472450149765 1104 28-29 11.189315069833249 1104 30-31 10.757429580044693 1104 32-33 10.801641665549273 1104 34-35 10.693178918639827 1104 36-37 10.959178479788935 1104 38-39 10.730715861046559 1104 40-41 10.098757209587028 1104 42-43 9.855157660599268 1104 44-45 10.13908635932735 1104 46-47 10.04869617039412 1104 48-49 10.074835128975561 1104 50-51 11.527040646016369 1104 52-53 10.737073462011072 1104 54-55 10.763408533485745 1104 56-57 10.269215518666059 1104 58-59 10.14017935010094 1104 60-61 9.791147554851317 1104 62-63 10.035900201106536 1104 64-65 9.86321458967631 1104 66-67 9.681167279056293 1104 68-69 9.371802162993664 1104 70-71 8.968463346663915 1104 72-73 9.083597977822286 1104 74-75 8.919914237767246 1104 76-77 8.423048838378433 1104 78-79 7.965430856056452 1104 80-81 7.705662938725672 1104 82-83 7.469806779127159 1104 84-85 7.0770520158054975 1104 86-87 6.905706370608071 1104 88-89 6.568573728495052 1104 90-91 6.171856143736596 1104 92-93 5.9393691019550925 1104 94-95 5.468291245468965 1104 96-97 4.95267513841908 1104 98-99 4.860903283380534 1104 100-101 4.449007963318756 1105 1 7.943237090606484 1105 2 9.07213667152282 1105 3 8.562429915675228 1105 4 10.89028878848806 1105 5 10.848839548610282 1105 6 10.50666054292714 1105 7 10.058238913742088 1105 8 9.70819003778163 1105 9 9.523506953652127 1105 10-11 8.942736166926949 1105 12-13 8.63396428426952 1105 14-15 9.377080794346892 1105 16-17 10.264200271061384 1105 18-19 10.731631888424797 1105 20-21 11.221878401813182 1105 22-23 11.591969222383664 1105 24-25 11.404732540729984 1105 26-27 11.347385404453185 1105 28-29 11.238958405401668 1105 30-31 11.005908551962936 1105 32-33 11.089112101771128 1105 34-35 11.016704381836117 1105 36-37 10.746167787890617 1105 38-39 10.563242663834426 1105 40-41 10.418950858725054 1105 42-43 10.319901640837006 1105 44-45 10.177495240074485 1105 46-47 9.988709055015402 1105 48-49 9.724377666486316 1105 50-51 11.269592546059757 1105 52-53 10.285533954285413 1105 54-55 10.204601851012775 1105 56-57 10.104747345287556 1105 58-59 10.011248992793739 1105 60-61 9.765899321373798 1105 62-63 9.636613310843487 1105 64-65 9.457739502135386 1105 66-67 9.162510508129376 1105 68-69 9.042650196043738 1105 70-71 8.83018353125584 1105 72-73 8.56496377689524 1105 74-75 8.199548355426332 1105 76-77 8.074776867688756 1105 78-79 7.943136516963017 1105 80-81 7.618451217518492 1105 82-83 7.408940383300488 1105 84-85 6.95534004114233 1105 86-87 6.607224257880786 1105 88-89 6.324233831601239 1105 90-91 6.017883458549383 1105 92-93 5.834709529134342 1105 94-95 5.399087097263683 1105 96-97 5.106010677622949 1105 98-99 4.809806702772036 1105 100-101 4.241599598248644 1106 1 7.746243763425241 1106 2 9.042116537013772 1106 3 8.636313684292745 1106 4 10.485212838634943 1106 5 11.210150660210338 1106 6 10.271783103715709 1106 7 9.496776027548442 1106 8 9.297312312105365 1106 9 8.686912709955973 1106 10-11 8.58768910084028 1106 12-13 8.213199207439562 1106 14-15 8.812172348768097 1106 16-17 10.21191310386645 1106 18-19 11.377357500840464 1106 20-21 11.143632947177519 1106 22-23 11.33945629155932 1106 24-25 10.924318064074068 1106 26-27 11.219067492333174 1106 28-29 11.128856680184438 1106 30-31 10.718520684180266 1106 32-33 10.95255034422306 1106 34-35 10.718036313907714 1106 36-37 11.151913306161163 1106 38-39 11.024058674457784 1106 40-41 10.87475632321442 1106 42-43 10.507736505937519 1106 44-45 10.064235714579329 1106 46-47 9.943342127639848 1106 48-49 10.067397523590412 1106 50-51 11.877256515901566 1106 52-53 11.196317198335766 1106 54-55 10.590635654726547 1106 56-57 10.43892454190986 1106 58-59 10.15594005297681 1106 60-61 10.023256916208112 1106 62-63 10.27734347692838 1106 64-65 10.16109976920345 1106 66-67 9.850604226999511 1106 68-69 9.54711110934024 1106 70-71 9.238574319340453 1106 72-73 9.056441663664835 1106 74-75 8.742963741090408 1106 76-77 8.575707662702024 1106 78-79 8.4618639400608 1106 80-81 8.091221742357453 1106 82-83 7.488289849263593 1106 84-85 7.259021890876854 1106 86-87 6.9837352628330915 1106 88-89 6.764245764760018 1106 90-91 6.376929599665241 1106 92-93 6.096395806131168 1106 94-95 5.594463451230681 1106 96-97 5.141199474776938 1106 98-99 4.538187343788881 1106 100-101 3.973836081875497 1107 1 7.1854558761488185 1107 2 8.145384998351076 1107 3 8.104684683712165 1107 4 9.74601922885164 1107 5 10.679293427007128 1107 6 9.65595054444114 1107 7 9.498778269096594 1107 8 8.05072998562127 1107 9 5.129851904883303 1107 10-11 7.4906172627450225 1107 12-13 7.530766660824899 1107 14-15 7.946426289307208 1107 16-17 8.836928320100228 1107 18-19 10.309048211304674 1107 20-21 10.480309125959462 1107 22-23 9.930843774348865 1107 24-25 10.207158921315862 1107 26-27 10.421963044953849 1107 28-29 10.441748346041958 1107 30-31 10.322939206368186 1107 32-33 10.762819519040615 1107 34-35 10.835711935023635 1107 36-37 11.095493301446634 1107 38-39 10.604847280656497 1107 40-41 10.014216936727237 1107 42-43 9.786787196260452 1107 44-45 9.330388480697295 1107 46-47 9.539433252615751 1107 48-49 9.923987133817093 1107 50-51 11.528026263995207 1107 52-53 10.418431534476909 1107 54-55 10.093065669359909 1107 56-57 9.772907912750316 1107 58-59 9.782710184304722 1107 60-61 9.51758903023778 1107 62-63 9.553817790350429 1107 64-65 9.492013255927876 1107 66-67 9.342612231382933 1107 68-69 9.255752956288841 1107 70-71 8.94539523064177 1107 72-73 8.432903317180376 1107 74-75 8.367989554983483 1107 76-77 8.282688254950783 1107 78-79 7.850430841508585 1107 80-81 7.462885663657518 1107 82-83 7.480547614698436 1107 84-85 7.226617471022797 1107 86-87 6.876076148287572 1107 88-89 6.688122133612408 1107 90-91 6.36659752630767 1107 92-93 5.932079456835757 1107 94-95 5.599307297585245 1107 96-97 5.190760144514636 1107 98-99 4.710787482360438 1107 100-101 4.375025368386652 1108 1 6.716731547868875 1108 2 7.867344440889013 1108 3 7.876118615617216 1108 4 9.189922549949802 1108 5 10.842199459350205 1108 6 9.46983649896999 1108 7 8.608876574009635 1108 8 5.983956276518807 1108 9 7.189221971279231 1108 10-11 6.2230771550819135 1108 12-13 5.068206941134967 1108 14-15 7.14411929748735 1108 16-17 8.50526144072586 1108 18-19 7.981410279796929 1108 20-21 8.223482977571368 1108 22-23 8.936794307499994 1108 24-25 8.686226149493116 1108 26-27 7.096731021418851 1108 28-29 8.923698447363453 1108 30-31 9.430941397210283 1108 32-33 9.918248404255149 1108 34-35 9.386773030427705 1108 36-37 8.806427858882099 1108 38-39 8.521556017226555 1108 40-41 9.030964767179487 1108 42-43 8.2013677003692 1108 44-45 7.645442299517313 1108 46-47 8.683326895749591 1108 48-49 9.069486413487905 1108 50-51 10.555758583953237 1108 52-53 9.904152858343188 1108 54-55 9.329364063072518 1108 56-57 9.104797286260595 1108 58-59 9.13280226645649 1108 60-61 9.070688511504553 1108 62-63 9.219626787518067 1108 64-65 8.997330901148988 1108 66-67 8.33833065185992 1108 68-69 8.446432482385529 1108 70-71 8.360427514576264 1108 72-73 8.159482289793718 1108 74-75 8.09689102679811 1108 76-77 7.602784802549246 1108 78-79 7.4479732611298335 1108 80-81 7.450495746964412 1108 82-83 7.3628342092180485 1108 84-85 6.962044918691751 1108 86-87 6.901378436107402 1108 88-89 6.674358129923464 1108 90-91 6.655979342091863 1108 92-93 6.1502079677507755 1108 94-95 5.787974825835985 1108 96-97 5.6358130776028315 1108 98-99 5.215195696065738 1108 100-101 4.57298388387748 1109 1 6.19870022634802 1109 2 7.802310241628039 1109 3 7.925999444362507 1109 4 8.77201645209033 1109 5 9.70029758650642 1109 6 8.73313474708998 1109 7 7.174857155977005 1109 8 7.915495051357045 1109 9 7.245153800794533 1109 10-11 5.655003958779943 1109 12-13 6.133493851220386 1109 14-15 7.43772210749113 1109 16-17 6.450394300542143 1109 18-19 5.53328812156354 1109 20-21 7.76837116832073 1109 22-23 8.84006988967333 1109 24-25 8.639013511224444 1109 26-27 8.92129417613847 1109 28-29 9.1520051386259 1109 30-31 6.9707559700277635 1109 32-33 5.6812820616816175 1109 34-35 7.579673283251001 1109 36-37 5.9395266784448815 1109 38-39 6.197792961144998 1109 40-41 7.187692997811471 1109 42-43 6.087360181888275 1109 44-45 6.49396855606653 1109 46-47 6.037819588083618 1109 48-49 6.9377937767713505 1109 50-51 8.665093939722981 1109 52-53 9.124371467616829 1109 54-55 9.104691135550823 1109 56-57 8.305228884013008 1109 58-59 7.922546227862799 1109 60-61 7.979457935617706 1109 62-63 8.377119055410379 1109 64-65 8.382263895172638 1109 66-67 8.082313076402013 1109 68-69 7.706177384340055 1109 70-71 7.140013414675982 1109 72-73 6.704605828481952 1109 74-75 5.664719807095468 1109 76-77 5.667857804696199 1109 78-79 6.203163604047527 1109 80-81 6.239128272808751 1109 82-83 6.132529661393283 1109 84-85 5.942684528031043 1109 86-87 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2313 60-61 -15.826420012716252 2313 62-63 -15.385142269935933 2313 64-65 -15.045665719203999 2313 66-67 -14.702037354148342 2313 68-69 -14.268738377546878 2313 70-71 -13.91783008962952 2313 72-73 -13.52489623067192 2313 74-75 -13.069274951421944 2313 76-77 -12.67096660563701 2313 78-79 -12.287850997225403 2313 80-81 -11.869819686756953 2313 82-83 -11.405096695776317 2313 84-85 -11.001905513152034 2313 86-87 -10.526146911245208 2313 88-89 -10.106715846794524 2313 90-91 -9.619649647769196 2313 92-93 -9.081673369087378 2313 94-95 -8.520511843334123 2313 96-97 -7.900799765055824 2313 98-99 -7.26264884017159 2313 100-101 -6.384582770271976 2314 1 -18.61597592490619 2314 2 -17.345948837368592 2314 3 -17.96991968704895 2314 4 -19.46347587288159 2314 5 -18.785288336368907 2314 6 -19.7464609099673 2314 7 -19.924356621634377 2314 8 -20.373535274363658 2314 9 -21.636538449299064 2314 10-11 -22.004861260239153 2314 12-13 -21.946625954095975 2314 14-15 -21.891742512501384 2314 16-17 -20.68850498811379 2314 18-19 -19.63537530037581 2314 20-21 -19.289472335984772 2314 22-23 -18.919570694377587 2314 24-25 -18.76439345245957 2314 26-27 -18.47724569656838 2314 28-29 -18.28790500738683 2314 30-31 -18.27596810335299 2314 32-33 -18.136389222481615 2314 34-35 -17.83365505819415 2314 36-37 -17.380203759593304 2314 38-39 -17.262334332003636 2314 40-41 -17.141397230567414 2314 42-43 -16.987892532450925 2314 44-45 -16.615353795112803 2314 46-47 -16.272925451227504 2314 48-49 -16.024392670252237 2314 50-51 -13.877399508423784 2314 52-53 -15.69535897042136 2314 54-55 -16.433309613232403 2314 56-57 -16.413603014152475 2314 58-59 -16.128932619011028 2314 60-61 -15.826420012716252 2314 62-63 -15.385142269935933 2314 64-65 -15.045665719203999 2314 66-67 -14.702037354148342 2314 68-69 -14.268738377546878 2314 70-71 -13.91783008962952 2314 72-73 -13.52489623067192 2314 74-75 -13.069274951421944 2314 76-77 -12.67096660563701 2314 78-79 -12.287850997225403 2314 80-81 -11.869819686756953 2314 82-83 -11.405096695776317 2314 84-85 -11.001905513152034 2314 86-87 -10.526146911245208 2314 88-89 -10.106715846794524 2314 90-91 -9.619649647769196 2314 92-93 -9.081673369087378 2314 94-95 -8.520511843334123 2314 96-97 -7.900799765055824 2314 98-99 -7.26264884017159 2314 100-101 -6.384582770271976 2315 1 -18.61597592490619 2315 2 -17.345948837368592 2315 3 -17.96991968704895 2315 4 -19.46347587288159 2315 5 -18.785288336368907 2315 6 -19.7464609099673 2315 7 -19.924356621634377 2315 8 -20.373535274363658 2315 9 -21.636538449299064 2315 10-11 -22.004861260239153 2315 12-13 -21.946625954095975 2315 14-15 -21.891742512501384 2315 16-17 -20.68850498811379 2315 18-19 -19.63537530037581 2315 20-21 -19.289472335984772 2315 22-23 -18.919570694377587 2315 24-25 -18.76439345245957 2315 26-27 -18.47724569656838 2315 28-29 -18.28790500738683 2315 30-31 -18.27596810335299 2315 32-33 -18.136389222481615 2315 34-35 -17.83365505819415 2315 36-37 -17.380203759593304 2315 38-39 -17.262334332003636 2315 40-41 -17.141397230567414 2315 42-43 -16.987892532450925 2315 44-45 -16.615353795112803 2315 46-47 -16.272925451227504 2315 48-49 -16.024392670252237 2315 50-51 -13.877399508423784 2315 52-53 -15.69535897042136 2315 54-55 -16.433309613232403 2315 56-57 -16.413603014152475 2315 58-59 -16.128932619011028 2315 60-61 -15.826420012716252 2315 62-63 -15.385142269935933 2315 64-65 -15.045665719203999 2315 66-67 -14.702037354148342 2315 68-69 -14.268738377546878 2315 70-71 -13.91783008962952 2315 72-73 -13.52489623067192 2315 74-75 -13.069274951421944 2315 76-77 -12.67096660563701 2315 78-79 -12.287850997225403 2315 80-81 -11.869819686756953 2315 82-83 -11.405096695776317 2315 84-85 -11.001905513152034 2315 86-87 -10.526146911245208 2315 88-89 -10.106715846794524 2315 90-91 -9.619649647769196 2315 92-93 -9.081673369087378 2315 94-95 -8.520511843334123 2315 96-97 -7.900799765055824 2315 98-99 -7.26264884017159 2315 100-101 -6.384582770271976 2316 1 -18.61597592490619 2316 2 -17.345948837368592 2316 3 -17.96991968704895 2316 4 -19.46347587288159 2316 5 -18.785288336368907 2316 6 -19.7464609099673 2316 7 -19.924356621634377 2316 8 -20.373535274363658 2316 9 -21.636538449299064 2316 10-11 -22.004861260239153 2316 12-13 -21.946625954095975 2316 14-15 -21.891742512501384 2316 16-17 -20.68850498811379 2316 18-19 -19.63537530037581 2316 20-21 -19.289472335984772 2316 22-23 -18.919570694377587 2316 24-25 -18.76439345245957 2316 26-27 -18.47724569656838 2316 28-29 -18.28790500738683 2316 30-31 -18.27596810335299 2316 32-33 -18.136389222481615 2316 34-35 -17.83365505819415 2316 36-37 -17.380203759593304 2316 38-39 -17.262334332003636 2316 40-41 -17.141397230567414 2316 42-43 -16.987892532450925 2316 44-45 -16.615353795112803 2316 46-47 -16.272925451227504 2316 48-49 -16.024392670252237 2316 50-51 -13.877399508423784 2316 52-53 -15.69535897042136 2316 54-55 -16.433309613232403 2316 56-57 -16.413603014152475 2316 58-59 -16.128932619011028 2316 60-61 -15.826420012716252 2316 62-63 -15.385142269935933 2316 64-65 -15.045665719203999 2316 66-67 -14.702037354148342 2316 68-69 -14.268738377546878 2316 70-71 -13.91783008962952 2316 72-73 -13.52489623067192 2316 74-75 -13.069274951421944 2316 76-77 -12.67096660563701 2316 78-79 -12.287850997225403 2316 80-81 -11.869819686756953 2316 82-83 -11.405096695776317 2316 84-85 -11.001905513152034 2316 86-87 -10.526146911245208 2316 88-89 -10.106715846794524 2316 90-91 -9.619649647769196 2316 92-93 -9.081673369087378 2316 94-95 -8.520511843334123 2316 96-97 -7.900799765055824 2316 98-99 -7.26264884017159 2316 100-101 -6.384582770271976 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 656080.0 3 26800.0 4 98087.0 5 52339.0 6 30619.0 7 30858.0 8 29362.0 9 28673.0 10 30664.0 11 32159.0 12 33555.0 13 33962.0 14 35036.0 15 35767.0 16 37344.0 17 37964.0 18 37411.0 19 38495.0 20 40638.0 21 42723.0 22 45442.0 23 48702.0 24 51455.0 25 54869.0 26 58733.0 27 62878.0 28 67528.0 29 72415.0 30 78321.0 31 84577.0 32 90832.0 33 96629.0 34 99622.0 35 93820.0 36 70510.0 37 30221.0 38 3871.0 39 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.559440834077456 21.161366868406255 14.40030012898105 22.878892168535238 2 14.416932634802054 21.26122355199971 42.42781325283389 21.894030560364342 3 17.991371197858946 27.13976161849868 30.967597316993263 23.90126986664911 4 11.923564672759404 17.910014960286308 40.88647387305063 29.27994649390365 5 12.110053324154666 38.55922775122871 36.49020998585619 12.84050893876044 6 28.360421915152088 39.673315836741665 18.87194760255717 13.09431464554908 7 24.954848421970873 33.6727024039907 24.315209888671983 17.057239285366443 8 23.537337456820936 37.73101678532746 21.442386719637003 17.289259038214603 9 24.900761051910095 17.298297577577504 22.304619196421662 35.496322174090736 10-11 23.16094996457713 27.810952327684912 30.060773432113614 18.967324275624343 12-13 23.765344292787017 25.3782463685758 30.21010769079757 20.646301647839614 14-15 21.148866990145564 27.225151318093143 27.571932369566305 24.054049322194988 16-17 20.622018855214282 30.26807023336155 28.284022957671763 20.825887953752407 18-19 20.475938056217355 29.15362480397581 30.26784436926451 20.10259277054233 20-21 21.133326012545787 28.41373572694711 30.35993020849206 20.093008052015048 22-23 20.791129638854823 28.256170204358693 30.084659056430436 20.86804110035605 24-25 20.734940338323238 28.409773561402258 30.217336399453316 20.63794970082119 26-27 20.704328537758595 28.574756090064085 29.99724210743599 20.72367326474133 28-29 20.748266394690408 28.65441511674628 30.063011679224477 20.534306809338837 30-31 20.953234165541275 28.498861840604402 29.98979236708846 20.558111626765868 32-33 20.67064052874164 28.687106928994467 29.90821445721486 20.73403808504904 34-35 20.8391738467229 28.675872559376035 29.934971768335128 20.54998182556594 36-37 20.943986209940334 28.503092909416683 29.815591261299147 20.737329619343832 38-39 20.888782371376397 28.582565777035366 29.922156334591527 20.60649551699671 40-41 20.938621220913316 28.63385679441523 29.90721408983487 20.520307894836584 42-43 20.913951312682165 28.657301477213064 29.558764430213184 20.86998277989158 44-45 20.917515407329194 28.809357680848752 29.57695174978975 20.696175162032308 46-47 21.039380467581275 28.743221226515153 29.214250889306225 21.003147416597347 48-49 20.988030489473495 28.78846890804662 29.27454847921764 20.948952123262245 50-51 21.234540055308052 28.94228765417759 28.892818081058664 20.93035420945569 52-53 20.77652105520657 29.137138044288292 29.074098349149597 21.01224255135555 54-55 20.731182930859568 29.081839212660864 29.146312529558998 21.040665326920568 56-57 20.74905311475869 29.180743869057835 29.14531229497176 20.92489072121171 58-59 20.735698850934178 29.127865926844247 29.145930985605055 20.990504236616513 60-61 20.751599793227705 29.24204797934187 29.188180029817012 20.818172197613418 62-63 20.699495540835 29.148425830743562 29.153664903110236 20.998413725311202 64-65 20.578572857769778 29.02715562131145 29.144120027747576 21.25015149317119 66-67 20.519884482887445 29.06654669774572 29.02158447802808 21.391984341338752 68-69 20.62976846630769 29.068941235022844 29.028331506689753 21.27295879197971 70-71 20.678199115345073 28.918215349069182 29.09578468103171 21.30780085455403 72-73 20.65402486604157 29.033193922278983 28.91023140369991 21.402549807979536 74-75 20.57936709569782 29.24936885609919 28.712896540411215 21.458367507791777 76-77 20.639890546054488 29.073163598015245 28.879343193937807 21.40760266199246 78-79 20.428150984515582 29.230769230769234 28.764709177100784 21.5763706076144 80-81 20.563370106086662 29.23948648017535 28.533403171891873 21.66374024184611 82-83 20.721852562483168 29.024086109957008 28.735810668331354 21.518250659228467 84-85 20.727944087439393 29.100051230099417 28.58248171446721 21.589522967993982 86-87 20.621644368937282 29.139179952285136 28.711394169144405 21.527781509633176 88-89 20.697424311004138 29.05511840806307 28.59717051189754 21.650286769035247 90-91 20.69058041782389 29.077522564301162 28.611272967806062 21.620624050068884 92-93 20.661253834870692 28.971199625560608 28.532512158410373 21.835034381158334 94-95 20.5589193965649 28.914038614127257 28.62027336411355 21.906768625194292 96-97 20.566996643091226 28.851150797764184 28.562130720100786 22.0197218390438 98-99 20.41413312966103 28.858231414737617 28.36764202482454 22.35999343077681 100-101 20.65913040930858 28.927934475877226 28.342966213114018 22.06996890170018 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 153260.0 1 139324.0 2 92432.5 3 46643.5 4 29073.5 5 23051.5 6 21590.5 7 21794.0 8 22293.0 9 21664.5 10 19554.0 11 16316.5 12 12851.0 13 9780.0 14 7347.5 15 6055.5 16 5741.0 17 6247.5 18 7260.5 19 8398.0 20 9813.0 21 11365.5 22 12740.0 23 14155.0 24 15906.5 25 17966.5 26 20550.0 27 23848.0 28 28017.0 29 33844.0 30 40471.5 31 47303.0 32 54484.0 33 60412.5 34 65242.0 35 69190.5 36 72913.5 37 75848.5 38 76436.0 39 75365.5 40 73279.0 41 71683.5 42 70697.5 43 70139.5 44 70004.0 45 69214.0 46 67885.0 47 66240.0 48 63841.0 49 61035.0 50 57522.0 51 53215.5 52 48462.0 53 43488.0 54 38641.5 55 33476.0 56 28028.5 57 22898.5 58 18283.0 59 14285.5 60 10910.0 61 8079.5 62 5942.5 63 4187.5 64 2776.0 65 1801.5 66 1147.5 67 699.5 68 414.0 69 279.5 70 181.5 71 105.0 72 66.0 73 45.5 74 31.5 75 23.0 76 17.5 77 13.0 78 9.0 79 3.5 80 2.5 81 3.0 82 3.0 83 2.0 84 1.5 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.56190208272392 2 29.63724901330019 3 23.41604818272364 4 29.75897833802257 5 32.918778689124835 6 25.45623486636785 7 24.401687563800852 8 20.345804119829356 9 12.07549298975945 10-11 15.75472381408947 12-13 15.815708525094369 14-15 20.003585452825483 16-17 23.400221769861147 18-19 27.415768869545943 20-21 29.487208216769435 22-23 29.11057560523524 24-25 27.59254049740955 26-27 29.047530059179984 28-29 26.002776324966874 30-31 27.90706794393088 32-33 26.87607018363841 34-35 24.919097218192814 36-37 27.91889273533636 38-39 28.036920560210998 40-41 25.0401462672675 42-43 25.917021577143057 44-45 24.583241129305595 46-47 27.93712012107306 48-49 23.302462157666287 50-51 28.85675275621682 52-53 22.461861546298557 54-55 23.59636124557671 56-57 25.742330792437095 58-59 25.0182974141122 60-61 24.56281285176754 62-63 24.38346017806415 64-65 22.90258012546684 66-67 23.41580808543622 68-69 24.420975379223663 70-71 22.894636906874666 72-73 22.758181615190477 74-75 19.985818253556342 76-77 22.288351239922417 78-79 21.411756043548845 80-81 22.456479365438874 82-83 23.333894894211134 84-85 20.248924864355036 86-87 21.81237837571784 88-89 20.653148655835345 90-91 21.778424617655073 92-93 21.771401771998015 94-95 20.795846476992477 96-97 19.703143713832848 98-99 19.1053014681549 100-101 20.773857567086186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2498987.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.74235283657288 #Duplication Level Percentage of deduplicated Percentage of total 1 90.20091731704147 71.02632457561016 2 6.490810197086058 10.222033334683509 3 1.6125968400515172 3.8093900808743704 4 0.6095592690271806 1.919925241461668 5 0.3173225647382135 1.2493362677811324 6 0.20131912874518754 0.9511405121043 7 0.12844297226379944 0.7079731288961967 8 0.09067908107306778 0.5712227357401359 9 0.06322859259064434 0.4480891332419191 >10 0.2731455865421953 3.567672037134021 >50 0.008664882489216099 0.46912774724993633 >100 0.003110216890374877 0.38479447755996626 >500 5.083786526623525E-5 0.02598007437639579 >1k 1.0167573053243459E-4 0.11468861800978068 >5k 0.0 0.0 >10k+ 5.083786526621729E-5 4.532302035276569 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 113220 4.530635813631684 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.001621457014382E-5 0.0 0.0 0.0 3 0.0 4.001621457014382E-5 0.0 0.0 0.0 4 0.0 4.001621457014382E-5 0.0 0.0 0.0 5 4.001621457014382E-5 4.001621457014382E-5 0.0 0.0 0.0 6 4.001621457014382E-5 4.001621457014382E-5 0.0 0.0 0.0 7 4.001621457014382E-5 4.001621457014382E-5 0.0 0.0 0.0 8 4.001621457014382E-5 4.001621457014382E-5 0.0 0.0 0.0 9 4.001621457014382E-5 4.001621457014382E-5 0.0 0.0 0.0 10-11 6.002432185521573E-5 4.001621457014382E-5 0.0 0.0 0.0 12-13 8.003242914028765E-5 4.001621457014382E-5 0.0 0.0 0.0 14-15 8.003242914028765E-5 4.001621457014382E-5 0.0 0.0 0.0 16-17 8.003242914028765E-5 4.001621457014382E-5 0.0 0.0 0.0 18-19 1.0004053642535956E-4 4.001621457014382E-5 0.0 0.0 0.0 20-21 1.2004864371043146E-4 4.001621457014382E-5 0.0 2.000810728507191E-5 0.0 22-23 1.4005675099550336E-4 4.001621457014382E-5 0.0 8.003242914028765E-5 0.0 24-25 1.600648582805753E-4 4.001621457014382E-5 0.0 1.2004864371043146E-4 0.0 26-27 1.600648582805753E-4 4.001621457014382E-5 0.0 1.4005675099550336E-4 0.0 28-29 1.600648582805753E-4 4.001621457014382E-5 0.0 4.0016214570143824E-4 0.0 30-31 1.600648582805753E-4 4.001621457014382E-5 0.0 0.0010804377933938832 0.0 32-33 1.600648582805753E-4 4.001621457014382E-5 0.0 0.0032613214874667217 0.0 34-35 1.600648582805753E-4 4.001621457014382E-5 0.0 0.006202513258372292 0.0 36-37 1.600648582805753E-4 4.001621457014382E-5 0.0 0.01056428064651797 0.0 38-39 1.600648582805753E-4 4.001621457014382E-5 0.0 0.01852750734597659 4.001621457014382E-5 40-41 1.600648582805753E-4 4.001621457014382E-5 0.0 0.026390693509009848 4.001621457014382E-5 42-43 1.600648582805753E-4 4.001621457014382E-5 0.0 0.03411382292104761 4.001621457014382E-5 44-45 1.600648582805753E-4 4.001621457014382E-5 0.0 0.044077860349013426 4.001621457014382E-5 46-47 1.600648582805753E-4 4.001621457014382E-5 0.0 0.057723389517432465 4.001621457014382E-5 48-49 1.600648582805753E-4 8.003242914028765E-5 0.0 0.07405000506205114 4.001621457014382E-5 50-51 1.600648582805753E-4 8.003242914028765E-5 0.0 0.09107690436164734 4.001621457014382E-5 52-53 1.600648582805753E-4 8.003242914028765E-5 0.0 0.11130510082685505 4.001621457014382E-5 54-55 1.600648582805753E-4 8.003242914028765E-5 0.0 0.13351409991328486 4.001621457014382E-5 56-57 1.600648582805753E-4 8.003242914028765E-5 0.0 0.15880434752161576 4.001621457014382E-5 58-59 1.600648582805753E-4 8.003242914028765E-5 0.0 0.18921667059492506 4.001621457014382E-5 60-61 1.600648582805753E-4 8.003242914028765E-5 0.0 0.22395074484180988 4.001621457014382E-5 62-63 1.600648582805753E-4 8.003242914028765E-5 0.0 0.2623463027218629 4.001621457014382E-5 64-65 1.600648582805753E-4 8.003242914028765E-5 0.0 0.299361301199246 4.001621457014382E-5 66-67 1.8007296556564722E-4 8.003242914028765E-5 0.0 0.344459575019798 4.001621457014382E-5 68-69 2.0008107285071912E-4 8.003242914028765E-5 0.0 0.39544023238216125 4.001621457014382E-5 70-71 2.0008107285071912E-4 8.003242914028765E-5 0.0 0.4506225922743896 6.002432185521573E-5 72-73 2.0008107285071912E-4 8.003242914028765E-5 0.0 0.5119274329958499 8.003242914028765E-5 74-75 2.0008107285071912E-4 1.2004864371043146E-4 0.0 0.5745528087981251 8.003242914028765E-5 76-77 2.0008107285071912E-4 1.2004864371043146E-4 0.0 0.6411798060574144 8.003242914028765E-5 78-79 2.0008107285071912E-4 1.2004864371043146E-4 0.0 0.7205519676572947 8.003242914028765E-5 80-81 2.0008107285071912E-4 1.2004864371043146E-4 0.0 0.8087076883553216 8.003242914028765E-5 82-83 2.0008107285071912E-4 1.2004864371043146E-4 0.0 0.9008450224030777 8.003242914028765E-5 84-85 2.0008107285071912E-4 1.2004864371043146E-4 0.0 0.9909015132931864 8.003242914028765E-5 86-87 2.0008107285071912E-4 1.2004864371043146E-4 0.0 1.0906419281092699 8.003242914028765E-5 88-89 2.0008107285071912E-4 1.2004864371043146E-4 0.0 1.203367604553365 8.003242914028765E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1840 0.0 53.208485 1 GTATCAA 3650 0.0 42.551525 1 ATCAACG 4125 0.0 36.77633 3 TCAACGC 4145 0.0 36.59777 4 CAACGCA 4345 0.0 35.020695 5 TATCAAC 4515 0.0 33.37553 2 AACGCAG 4710 0.0 33.07639 6 ACGCAGA 5245 0.0 29.18653 7 CGCAGAG 5410 0.0 28.084997 8 GTACATG 3650 0.0 24.295095 1 GCAGAGT 5810 0.0 24.117575 9 ACATGGG 3625 0.0 23.218506 3 TACATGG 3845 0.0 22.261723 2 GAGTACT 3925 0.0 20.868433 12-13 CAGAGTA 5575 0.0 19.950403 10-11 AGAGTAC 5225 0.0 19.928995 10-11 GTGGTAT 1125 0.0 19.136482 1 GAGTACA 2940 0.0 17.957888 1 GTACTTT 4530 0.0 17.59754 14-15 AGTACTT 4520 0.0 17.430527 12-13 >>END_MODULE