FastQCFastQC Report
Sat 18 Jun 2016
SRR1931001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2498987
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51890.20764413740447632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47080.18839633819623713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.10748355233540631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.054.5647771
GTATCAA58450.039.911421
ATCAACG69350.033.0198173
TCAACGC69350.033.0198174
CAACGCA71200.032.119395
AACGCAG74250.031.1182636
TATCAAC73750.031.0510652
ACGCAGA85800.026.4868957
CGCAGAG86800.026.1817478
GTACATG60000.023.35991
GCAGAGT100750.022.1798539
ACATGGG60500.021.5163843
TACATGG63450.021.2656292
AGAGTAC94100.019.68857410-11
GAGTACT70350.019.65881312-13
CAGAGTA100400.018.71303710-11
GTACTTT76350.018.02070614-15
AGTACTT78250.017.79535312-13
ATTATAC18200.016.4453563
CATGGGT18750.016.216344
Produced by FastQC (version 0.11.5)