##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931001_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2498987 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34679772243713 31.0 31.0 34.0 30.0 34.0 2 31.507145095192573 31.0 31.0 34.0 30.0 34.0 3 31.57557162162108 31.0 31.0 34.0 30.0 34.0 4 35.346694080441395 37.0 35.0 37.0 33.0 37.0 5 35.218966725317095 37.0 35.0 37.0 33.0 37.0 6 35.24482080138872 37.0 35.0 37.0 32.0 37.0 7 35.130516485279834 37.0 35.0 37.0 32.0 37.0 8 35.13268216281237 37.0 35.0 37.0 32.0 37.0 9 36.63582723719651 39.0 35.0 39.0 32.0 39.0 10-11 36.57899300796683 38.5 35.0 39.0 32.0 39.0 12-13 36.47129356815381 38.0 35.0 39.0 32.0 39.0 14-15 37.54167408633978 39.0 36.0 41.0 32.0 41.0 16-17 37.49800559186583 39.0 36.0 41.0 32.0 41.0 18-19 37.522564343071814 39.0 36.0 41.0 32.0 41.0 20-21 37.50523692200079 39.0 36.0 41.0 32.0 41.0 22-23 37.40679563359073 39.0 36.0 41.0 32.0 41.0 24-25 37.28431480435873 39.0 36.0 41.0 31.5 41.0 26-27 37.042605063571756 39.0 36.0 40.0 31.0 41.0 28-29 36.856467440606934 39.0 36.0 40.0 30.5 41.0 30-31 36.64072902340028 38.5 35.5 40.0 30.0 41.0 32-33 36.48410195811343 38.0 35.0 40.0 30.0 41.0 34-35 36.569957146635815 38.5 35.0 40.0 30.0 41.0 36-37 36.64035227074011 39.0 35.0 40.0 30.0 41.0 38-39 36.63545088469848 39.0 35.0 40.0 30.0 41.0 40-41 36.530730051816995 39.0 35.0 40.0 30.0 41.0 42-43 36.40421858937242 39.0 35.0 40.0 30.0 41.0 44-45 36.25438467667098 39.0 35.0 40.0 30.0 41.0 46-47 36.031766671855436 38.0 35.0 40.0 29.5 41.0 48-49 35.83865282212352 38.0 35.0 40.0 28.5 41.0 50-51 35.59514035087017 38.0 34.5 40.0 27.5 41.0 52-53 35.395607700240134 38.0 34.0 40.0 27.0 41.0 54-55 35.25404573933358 38.0 34.0 40.0 27.0 41.0 56-57 35.09674560131765 38.0 34.0 40.0 27.0 41.0 58-59 34.90746270388762 38.0 34.0 40.0 26.0 41.0 60-61 34.61106880507982 38.0 33.0 40.0 26.0 41.0 62-63 34.37163898811799 37.0 33.0 40.0 26.0 41.0 64-65 34.06923425371961 37.0 33.0 40.0 25.0 41.0 66-67 33.766174653969784 36.5 32.0 39.0 25.0 41.0 68-69 33.428380979973085 36.0 32.0 39.0 23.5 40.5 70-71 33.0588968650097 36.0 31.5 39.0 23.0 40.0 72-73 32.64027944122959 35.0 31.0 38.5 22.0 40.0 74-75 32.130412443121955 35.0 31.0 37.5 22.0 40.0 76-77 30.729525003531432 33.5 29.5 36.0 20.5 39.0 78-79 31.468308358546885 34.0 30.5 36.0 21.0 39.0 80-81 31.43503847759112 34.0 31.0 36.0 21.0 39.0 82-83 31.150997384140055 34.0 31.0 36.0 20.5 37.5 84-85 30.79373842280892 34.0 30.0 35.5 20.0 37.0 86-87 30.438496278692128 34.0 30.0 35.0 18.5 37.0 88-89 30.07758003543036 34.0 30.0 35.0 18.0 36.0 90-91 29.73598141967125 34.0 29.5 35.0 14.0 36.0 92-93 29.40895931031254 34.0 29.0 35.0 7.5 35.5 94-95 29.022280828191583 34.0 29.0 35.0 2.0 35.0 96-97 28.59383962381557 34.0 29.0 35.0 2.0 35.0 98-99 28.099237210917863 34.0 29.0 35.0 2.0 35.0 100-101 26.964111257881694 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3448351554426452 1101 2 0.4373130933477327 1101 3 0.28528000733001235 1101 4 0.15429495235560609 1101 5 0.23343689888261565 1101 6 0.4128531418477195 1101 7 0.4423191276691796 1101 8 0.44919203604435864 1101 9 0.5659514917195594 1101 10-11 0.5175389693635353 1101 12-13 0.5529287870581925 1101 14-15 0.6466797407740046 1101 16-17 0.7735431375655679 1101 18-19 0.6591594190458565 1101 20-21 0.6055285776394186 1101 22-23 0.6475365133255266 1101 24-25 0.5993102822513023 1101 26-27 0.5594732974512766 1101 28-29 0.6434509131883956 1101 30-31 0.6542494529051055 1101 32-33 0.6119814569459621 1101 34-35 0.570241427072375 1101 36-37 0.6672563025203786 1101 38-39 0.8627879355956338 1101 40-41 0.8762845712411931 1101 42-43 0.729658329965531 1101 44-45 0.6553854924453333 1101 46-47 0.6808740172273033 1101 48-49 0.7687456867689875 1101 50-51 0.6985669385101687 1101 52-53 0.6004500000946891 1101 54-55 0.8476216161169603 1101 56-57 0.8545181411167064 1101 58-59 0.8375644360824737 1101 60-61 0.7887061493545744 1101 62-63 0.5089740038750463 1101 64-65 0.5949141412018761 1101 66-67 0.5014276337979808 1101 68-69 0.6006502773115017 1101 70-71 0.6720459876318046 1101 72-73 0.5659327815970414 1101 74-75 0.7469847932555211 1101 76-77 0.6617559324805988 1101 78-79 0.7227265507179794 1101 80-81 0.8216953400236662 1101 82-83 0.8695862062621238 1101 84-85 0.8292999155038494 1101 86-87 0.8537879654911116 1101 88-89 0.9090643100633962 1101 90-91 1.0259873805276172 1101 92-93 0.8229325748906469 1101 94-95 1.182940569798749 1101 96-97 1.3650432151814265 1101 98-99 1.3129016088086232 1101 100-101 1.418036369304076 1102 1 0.3491228380737006 1102 2 0.45971762346922773 1102 3 0.4386843488241148 1102 4 0.28636141813888116 1102 5 0.22885478516423063 1102 6 0.312197924959726 1102 7 0.42610674662651604 1102 8 0.44383339025500845 1102 9 0.443669234280506 1102 10-11 0.4405945512503209 1102 12-13 0.4901752292295285 1102 14-15 0.5089563681161664 1102 16-17 0.48950738262033155 1102 18-19 0.5112259901540739 1102 20-21 0.46491595032789945 1102 22-23 0.4926016089270604 1102 24-25 0.5423641980890181 1102 26-27 0.6183372530539373 1102 28-29 0.6133799241776217 1102 30-31 0.4510154026919153 1102 32-33 0.4690756184014404 1102 34-35 0.5867695435655875 1102 36-37 0.5964583128862628 1102 38-39 0.5168840704575217 1102 40-41 0.5329915677855794 1102 42-43 0.37666944749189213 1102 44-45 0.5094357630825499 1102 46-47 0.46348764608327286 1102 48-49 0.548896387610732 1102 50-51 0.3858002150637816 1102 52-53 0.2705861660944606 1102 54-55 0.45962870735804273 1102 56-57 0.4573748886633453 1102 58-59 0.3183210904644227 1102 60-61 0.31088470960091996 1102 62-63 0.2466330325684325 1102 64-65 0.22696456886901473 1102 66-67 0.3540304046099436 1102 68-69 0.4479777047881015 1102 70-71 0.3788321215074646 1102 72-73 0.3547361657831729 1102 74-75 0.396373142593454 1102 76-77 0.3910263083087173 1102 78-79 0.4057580802180567 1102 80-81 0.37086126223449867 1102 82-83 0.38703400099721463 1102 84-85 0.5098766557352015 1102 86-87 0.4967818939869666 1102 88-89 0.4384087963505685 1102 90-91 0.34609405554368067 1102 92-93 0.38170939804145476 1102 94-95 0.515376965502643 1102 96-97 0.6708620830914995 1102 98-99 0.632501527229671 1102 100-101 0.7205334321744523 1103 1 0.31434483856874706 1103 2 0.3936014666829344 1103 3 0.36343131266661644 1103 4 0.2811314821449429 1103 5 0.3386361474289714 1103 6 0.3506918508021215 1103 7 0.38712545094135464 1103 8 0.3148701705553165 1103 9 0.47422704703098617 1103 10-11 0.3976510339448538 1103 12-13 0.4254617578602833 1103 14-15 0.5020088142705532 1103 16-17 0.4842069668665232 1103 18-19 0.5097437654873218 1103 20-21 0.6144277820216146 1103 22-23 0.4767140276757189 1103 24-25 0.5364151842835767 1103 26-27 0.6099888563993829 1103 28-29 0.647957693602244 1103 30-31 0.5960108483788957 1103 32-33 0.6776087979384116 1103 34-35 0.5889390610540843 1103 36-37 0.47864732516847397 1103 38-39 0.46331269672562314 1103 40-41 0.4775958584828075 1103 42-43 0.41439055278650727 1103 44-45 0.4200235120726461 1103 46-47 0.41131511900154294 1103 48-49 0.2865943681736738 1103 50-51 0.30826065580335893 1103 52-53 0.38717688659721716 1103 54-55 0.3451482337831848 1103 56-57 0.4435694545663793 1103 58-59 0.4843448404544404 1103 60-61 0.45934364431547436 1103 62-63 0.3714895394625515 1103 64-65 0.43367218442971733 1103 66-67 0.5004836604063314 1103 68-69 0.43004498209509023 1103 70-71 0.42456980044774895 1103 72-73 0.3867069518459303 1103 74-75 0.2660683506454049 1103 76-77 0.3365453003775194 1103 78-79 0.4942725204306413 1103 80-81 0.5210749740641063 1103 82-83 0.5179104621086665 1103 84-85 0.41557565833143784 1103 86-87 0.35947043118338584 1103 88-89 0.4525888940947844 1103 90-91 0.38818920536921553 1103 92-93 0.21212757180799358 1103 94-95 0.2965990384736088 1103 96-97 0.5252447571703662 1103 98-99 0.3050143287494542 1103 100-101 0.5674910098081334 1104 1 0.31370504946442423 1104 2 0.4714935331174779 1104 3 0.3745204889155751 1104 4 0.2464573803625001 1104 5 0.346675341282662 1104 6 0.35649358840432654 1104 7 0.334637465795538 1104 8 0.41002450538229596 1104 9 0.44253010122648107 1104 10-11 0.4033904778760373 1104 12-13 0.4317520644233852 1104 14-15 0.5714904827779392 1104 16-17 0.5399590500932732 1104 18-19 0.6718192581941551 1104 20-21 0.5659317077266337 1104 22-23 0.581598964945691 1104 24-25 0.396310849908005 1104 26-27 0.6236331264349246 1104 28-29 0.5808341565254764 1104 30-31 0.6420941714302657 1104 32-33 0.7057137763161876 1104 34-35 0.6059571201999674 1104 36-37 0.5484749191581884 1104 38-39 0.5788788920268075 1104 40-41 0.4863897988433834 1104 42-43 0.6276512803886192 1104 44-45 0.6069474084665387 1104 46-47 0.4816690493710638 1104 48-49 0.45384008510762186 1104 50-51 0.4619963199832924 1104 52-53 0.4516249556438865 1104 54-55 0.47612955470994933 1104 56-57 0.5757961975101935 1104 58-59 0.577516912426205 1104 60-61 0.37786171700346216 1104 62-63 0.47040376378596704 1104 64-65 0.4021908589889236 1104 66-67 0.507085435324548 1104 68-69 0.41914705042552924 1104 70-71 0.48841389172753225 1104 72-73 0.34833690938391726 1104 74-75 0.4595994387984774 1104 76-77 0.5051808884207425 1104 78-79 0.6374549746809066 1104 80-81 0.40096766042600507 1104 82-83 0.5967569458265451 1104 84-85 0.5937325481017304 1104 86-87 0.7442482052830606 1104 88-89 0.7525852828674786 1104 90-91 0.6770764056749634 1104 92-93 0.6113061680637557 1104 94-95 0.7940314558592583 1104 96-97 0.8195105796123379 1104 98-99 0.9813532665069431 1104 100-101 1.1454816326545654 1105 1 0.33544470707241203 1105 2 0.3201214378180275 1105 3 0.2872003002332981 1105 4 0.181811641709956 1105 5 0.2670201875071925 1105 6 0.21643848546613498 1105 7 0.2610112045415818 1105 8 0.24871474418604578 1105 9 0.3827697084354398 1105 10-11 0.42461876794654785 1105 12-13 0.3859072087033937 1105 14-15 0.5036204479953099 1105 16-17 0.5009987747538815 1105 18-19 0.4075038157856312 1105 20-21 0.385007740139784 1105 22-23 0.3286295126005214 1105 24-25 0.4839573175204208 1105 26-27 0.37175857473063445 1105 28-29 0.45843273775629 1105 30-31 0.42101868537179854 1105 32-33 0.49777804749510324 1105 34-35 0.4964370358217778 1105 36-37 0.40245103732272725 1105 38-39 0.4279735355595875 1105 40-41 0.38205694320519257 1105 42-43 0.36999004366949606 1105 44-45 0.3830190452078739 1105 46-47 0.42724847020587475 1105 48-49 0.3819455052675167 1105 50-51 0.170798546889948 1105 52-53 0.2971257832105465 1105 54-55 0.34793239370915785 1105 56-57 0.26172873293194243 1105 58-59 0.3642683225033778 1105 60-61 0.42838228784184906 1105 62-63 0.2525190475471035 1105 64-65 0.34611668038352406 1105 66-67 0.3964997401758623 1105 68-69 0.25362181219881563 1105 70-71 0.17290545317710126 1105 72-73 0.34485681992199346 1105 74-75 0.451052347845021 1105 76-77 0.33947750058341697 1105 78-79 0.39870367270599516 1105 80-81 0.58432378288542 1105 82-83 0.4868670494440259 1105 84-85 0.4698042578851336 1105 86-87 0.4562864150511281 1105 88-89 0.5770572982589073 1105 90-91 0.5895430071881052 1105 92-93 0.5054603920136636 1105 94-95 0.7177736348795314 1105 96-97 0.739835704477759 1105 98-99 0.9135758490700461 1105 100-101 0.8664279803319026 1106 1 0.29518690597478425 1106 2 0.3082430697529901 1106 3 0.3066699865154696 1106 4 0.15908686927477333 1106 5 0.27603951568674745 1106 6 0.23520538376378397 1106 7 0.3102162861862183 1106 8 0.29792845428966785 1106 9 0.3356203571445562 1106 10-11 0.33145686483638315 1106 12-13 0.35992066305949066 1106 14-15 0.43740630086023913 1106 16-17 0.45073368692263927 1106 18-19 0.5192048258761801 1106 20-21 0.475296366407143 1106 22-23 0.31728287332059324 1106 24-25 0.38059571304121675 1106 26-27 0.3448569810111195 1106 28-29 0.3531118259525172 1106 30-31 0.47954099274253537 1106 32-33 0.5116306069764605 1106 34-35 0.449725363113032 1106 36-37 0.45781360301226215 1106 38-39 0.4235712957842068 1106 40-41 0.3389042941172846 1106 42-43 0.37713354486518114 1106 44-45 0.38082957373981685 1106 46-47 0.2579527120857392 1106 48-49 0.3767425444981072 1106 50-51 0.35846968698117365 1106 52-53 0.2891284959980567 1106 54-55 0.5301492691629619 1106 56-57 0.6932506938700485 1106 58-59 0.6207872311241189 1106 60-61 0.5947249558735379 1106 62-63 0.35323300938602387 1106 64-65 0.32158899144292974 1106 66-67 0.3755772371116777 1106 68-69 0.39377602864630035 1106 70-71 0.24789668450565472 1106 72-73 0.394623829958185 1106 74-75 0.4385464751001251 1106 76-77 0.5314775385486392 1106 78-79 0.5037709078155395 1106 80-81 0.42594216253847605 1106 82-83 0.6445148193540895 1106 84-85 0.7156946307251602 1106 86-87 0.6639544402686575 1106 88-89 0.7330809764760602 1106 90-91 0.6216818598015159 1106 92-93 0.7720258468564083 1106 94-95 0.788993366784684 1106 96-97 0.620204709154816 1106 98-99 0.6182295221403251 1106 100-101 0.6797458773521043 1107 1 0.11939035582794943 1107 2 0.10311477649456435 1107 3 0.20077502421306193 1107 4 0.07152771929870738 1107 5 0.14409164013076037 1107 6 0.22852298673365112 1107 7 0.19277947043339339 1107 8 0.23510968314268155 1107 9 0.2445051196295296 1107 10-11 0.3091703453740635 1107 12-13 0.3081810286443627 1107 14-15 0.26595414657092675 1107 16-17 0.25089584543096777 1107 18-19 0.35513823763219676 1107 20-21 0.34839498163677973 1107 22-23 0.3474967283566386 1107 24-25 0.23290193555711625 1107 26-27 0.2520040854012109 1107 28-29 0.20254830258023304 1107 30-31 0.3485119628894964 1107 32-33 0.3789038034625136 1107 34-35 0.1793490717381232 1107 36-37 0.16690255661371367 1107 38-39 0.21475465906308955 1107 40-41 0.28838954862007427 1107 42-43 0.26107456803195106 1107 44-45 0.2060649348636474 1107 46-47 0.268748361431534 1107 48-49 0.1594065353990075 1107 50-51 0.18717664079284901 1107 52-53 0.13405273275892426 1107 54-55 0.15357046854875733 1107 56-57 0.27133131169639313 1107 58-59 0.1801464393586656 1107 60-61 0.1474345625371214 1107 62-63 -0.07185808035982433 1107 64-65 0.04389537435583435 1107 66-67 0.08589995875097856 1107 68-69 0.08704296334781247 1107 70-71 0.05247964226652613 1107 72-73 0.024214800408756787 1107 74-75 0.16071802428028548 1107 76-77 0.08659904357665127 1107 78-79 0.30277140533267755 1107 80-81 0.046149406926517145 1107 82-83 0.22587784732875704 1107 84-85 0.23028173589432654 1107 86-87 0.23774458140943366 1107 88-89 0.38701455550323516 1107 90-91 0.3334160750892394 1107 92-93 0.29861315424037116 1107 94-95 0.4272005922687896 1107 96-97 0.3103029437279865 1107 98-99 0.5068350191175561 1107 100-101 0.6177237207971835 1108 1 0.0986930952861158 1108 2 0.09359245194940513 1108 3 0.10656145644283654 1108 4 0.0333505553893616 1108 5 0.17030604778982195 1108 6 0.16913862977636995 1108 7 0.18424404356584034 1108 8 0.21699533544737903 1108 9 0.2592560917414275 1108 10-11 0.1618255316734647 1108 12-13 0.21020975069180992 1108 14-15 0.20790723051650417 1108 16-17 0.09052652921876359 1108 18-19 0.23039444192892944 1108 20-21 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70-71 -0.9408198258273544 2316 72-73 -1.7242851913403712 2316 74-75 -2.802108045536748 2316 76-77 -1.8079906773055647 2316 78-79 -1.8445260678840754 2316 80-81 -1.4620996300135474 2316 82-83 -0.9769807125826482 2316 84-85 -1.0820492816946974 2316 86-87 -0.9204268973482428 2316 88-89 -1.055586705596479 2316 90-91 -1.070404077936903 2316 92-93 -1.0028841953966818 2316 94-95 -1.4383934612371903 2316 96-97 -1.2184165028402916 2316 98-99 -1.2894670324155442 2316 100-101 -1.7948226445110258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 15.0 4 31.0 5 55.0 6 115.0 7 311.0 8 756.0 9 1496.0 10 2605.0 11 4204.0 12 5941.0 13 7702.0 14 9558.0 15 12058.0 16 14194.0 17 16515.0 18 14668.0 19 11754.0 20 11570.0 21 12328.0 22 13448.0 23 16342.0 24 19959.0 25 25217.0 26 32864.0 27 42485.0 28 54412.0 29 68085.0 30 83331.0 31 101029.0 32 121968.0 33 148334.0 34 183167.0 35 230261.0 36 302188.0 37 418155.0 38 424027.0 39 87820.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.55472140574696 22.358932083709334 15.250641786236663 22.83570472430704 2 14.425773951280457 22.262592178028704 41.449908817435976 21.86172505325486 3 17.812783871497 27.359789990115768 31.75503115333363 23.072394985053606 4 12.156177520589864 18.76397477185396 40.554797941534645 28.52504976602153 5 11.626672962682102 39.257043500531225 36.83265070920212 12.283632827584551 6 28.124817425363492 40.87282881241852 18.398041119209534 12.60431264300846 7 24.62405766816714 34.04479495091411 24.134499299116 17.196648081802746 8 23.36978313395722 38.40706661405815 21.19852162617919 17.024628625805434 9 24.381319310584647 17.129380825110335 22.1869101359871 36.30238972831791 10-11 22.971327982098348 28.113791708400242 30.058479695972807 18.8564006135286 12-13 23.44836127598903 25.57862445863064 30.380250077331333 20.592764188049 14-15 20.731220290461692 27.446661387194094 27.760648614818724 24.06146970752549 16-17 20.17489486740027 30.67360894634506 28.496586816978244 20.65490936927643 18-19 20.068371704214545 29.501934183731247 30.705121715319045 19.72457239673516 20-21 20.589002663879405 28.620437001072833 30.870768835532157 19.919791499515604 22-23 20.359647095662126 28.47576658853471 30.690812542329816 20.47377377347335 24-25 20.32189261975934 28.620211527149188 30.6694958322829 20.38840002080857 26-27 20.279929601418857 28.79330781291393 30.530922632791686 20.39583995287553 28-29 20.353772126764444 28.872362600523456 30.508489158483165 20.265376114228935 30-31 20.63639561505557 28.592061142385834 30.519888763629915 20.25165447892868 32-33 20.416548761788654 28.79615350594741 30.34071074327902 20.446586988984915 34-35 20.576899679504628 28.778387533658417 30.444999503728443 20.199713283108515 36-37 20.640905975980033 28.590405018082503 30.4723060069929 20.296382998944562 38-39 20.48327356223224 28.766859892323982 30.489504109279874 20.260362436163902 40-41 20.52707937623965 28.755966778720822 30.45222565323184 20.264728191807688 42-43 20.504794796170884 28.80921807893189 30.133667587345975 20.55231953755125 44-45 20.51175795087119 28.953875232436708 30.124337756725883 20.41002905996622 46-47 20.647020180160496 28.896435429949737 29.834477020502327 20.62206736938744 48-49 20.63361298833989 28.954768485714865 29.799936647905827 20.611681878039416 50-51 20.708814484129967 29.117649476244516 29.497377964962418 20.6761580746631 52-53 20.755117979797898 29.12845162294459 29.463671011865785 20.652759385391725 54-55 20.703217553903393 29.102469278107566 29.547448433967944 20.646864734021094 56-57 20.753649682139145 29.197737246097 29.404695468469335 20.643917603294522 58-59 20.69113397209521 29.090968034623536 29.563465579681502 20.65443241359975 60-61 20.728590743100536 29.230892818043024 29.554283113449436 20.486233325407003 62-63 20.685656456180133 29.212790882141356 29.5464796715177 20.555072990160813 64-65 20.534865800174657 29.0366550910651 29.5467664599318 20.881712648828447 66-67 20.567073009280914 29.11711750022667 29.461109938771795 20.854699551720625 68-69 20.58583597884341 29.191828848953893 29.440971812004076 20.781363360198625 70-71 20.659973751385834 28.969039952123556 29.49842444137462 20.872561855115993 72-73 20.647630560511725 29.079605783819996 29.38823223378814 20.88453142188013 74-75 20.590997467474715 29.27693925370053 29.25988588689445 20.872177391930308 76-77 20.526425543689673 29.20153913402657 29.372115126262727 20.89992019602103 78-79 20.463934801813238 29.258426651539665 29.34254559861216 20.93509294803494 80-81 20.529726857213284 29.29360148476222 29.187578481832233 20.989093176192263 82-83 20.666994266076614 29.264317901613733 29.245870426696897 20.822817405612753 84-85 20.67825883047811 29.271480804021788 29.100171389447006 20.950088976053095 86-87 20.505448807856943 29.359116313930407 29.207674949889693 20.927759928322956 88-89 20.6030807301603 29.205011393623533 29.1523300155203 21.03957786069587 90-91 20.593847901735145 29.20943070150392 29.274661007504086 20.92206038925685 92-93 20.635227481030906 29.197394636383358 29.15298866777291 21.014389214812823 94-95 20.434228020732977 29.137996752593875 29.197742622555566 21.230032604117582 96-97 20.508610088807984 28.956413138603764 29.291628968057857 21.243347804530398 98-99 20.36693268112239 29.141067960737693 29.15333293050344 21.33866642763648 100-101 20.403527509346787 29.04594941870446 29.15179230624249 21.398730765706265 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 144.0 1 113.5 2 141.5 3 205.0 4 176.0 5 167.5 6 271.0 7 557.0 8 948.0 9 1264.5 10 1269.0 11 1148.5 12 1222.0 13 1345.5 14 1548.0 15 1844.5 16 2096.5 17 2396.5 18 2708.5 19 2983.0 20 3490.5 21 4158.5 22 4919.5 23 5988.0 24 7754.5 25 10471.0 26 14113.0 27 19220.0 28 25987.0 29 35511.5 30 47032.5 31 58589.5 32 70311.5 33 80401.0 34 87962.5 35 93246.5 36 100116.0 37 107157.5 38 107658.5 39 104225.5 40 100442.0 41 97779.5 42 96825.5 43 97750.0 44 98396.5 45 98215.0 46 97170.5 47 94788.5 48 92126.5 49 88076.0 50 82339.5 51 75246.0 52 68297.0 53 61850.5 54 54760.0 55 46149.5 56 36937.0 57 28690.5 58 21945.5 59 16368.0 60 11686.5 61 8240.5 62 5856.5 63 3736.5 64 2049.5 65 1126.5 66 601.5 67 327.5 68 166.0 69 92.0 70 51.0 71 29.0 72 23.5 73 17.0 74 9.0 75 6.0 76 5.0 77 5.5 78 3.0 79 1.5 80 2.0 81 2.0 82 1.0 83 0.5 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00408165388615467 2 0.006162497043802149 3 0.002280924230498198 4 0.1725899334410303 5 0.002080843157647479 6 3.601459311312944E-4 7 0.0 8 0.0010004053642535956 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.8015403841636634E-4 24-25 6.80275647692445E-4 26-27 0.0029211836636204988 28-29 2.2008918013579102E-4 30-31 0.0065626591895035875 32-33 0.020168172143352488 34-35 0.014205756172401059 36-37 9.803972569685235E-4 38-39 3.601459311312944E-4 40-41 0.0018607539775116876 42-43 0.011544677903486494 44-45 0.00754305644647211 46-47 0.010824386041223905 48-49 0.010144110393531459 50-51 0.19353842176850058 52-53 0.1144663817778964 54-55 0.017727183054573714 56-57 0.006942813227919953 58-59 0.018227385736700512 60-61 0.12351004627074891 62-63 0.2379164037267901 64-65 0.2440989088778773 66-67 0.25390288144756257 68-69 0.24976120323955264 70-71 0.23621571460755897 72-73 0.16252585547663914 74-75 0.2725304293299645 76-77 0.21516718574366334 78-79 0.005782343005385783 80-81 4.001621457014382E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 6.002432185521573E-5 90-91 0.005942407863666358 92-93 0.017967280341994574 94-95 0.0027811269126249956 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2498987.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.81399029665131 #Duplication Level Percentage of deduplicated Percentage of total 1 76.33879211486877 45.66127770816842 2 14.217543557492233 17.008160247801154 3 4.272334330648077 7.666360925923028 4 1.771292879043162 4.23792380318461 5 0.941915116215771 2.8169850810799653 6 0.5711836895079324 2.049886539709978 7 0.3695003295845978 1.547090238986781 8 0.2650067389312106 1.2680888408782909 9 0.1838698008586117 0.9898187835963789 >10 0.9805260640096384 10.485432940991908 >50 0.05881774652967989 2.4257983516821917 >100 0.02807865689430229 2.9578504957814484 >500 8.709812005121006E-4 0.3337405069537293 >1k 2.0099566165401216E-4 0.3436389105544865 >5k 6.699855388467071E-5 0.2079466247075487 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5189 0.20764413740447632 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4708 0.18839633819623713 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2686 0.10748355233540631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.001621457014382E-5 0.0 5 0.0 0.0 0.0 8.003242914028765E-5 0.0 6 0.0 0.0 0.0 8.003242914028765E-5 0.0 7 0.0 0.0 0.0 8.003242914028765E-5 0.0 8 0.0 0.0 0.0 8.003242914028765E-5 0.0 9 0.0 0.0 0.0 8.003242914028765E-5 0.0 10-11 0.0 0.0 0.0 1.0004053642535956E-4 0.0 12-13 0.0 0.0 0.0 1.600648582805753E-4 0.0 14-15 0.0 0.0 0.0 1.600648582805753E-4 0.0 16-17 8.003242914028765E-5 0.0 0.0 1.600648582805753E-4 0.0 18-19 1.2004864371043146E-4 0.0 0.0 1.600648582805753E-4 0.0 20-21 1.2004864371043146E-4 0.0 0.0 1.8007296556564722E-4 0.0 22-23 1.600648582805753E-4 0.0 0.0 3.001216092760787E-4 0.0 24-25 1.600648582805753E-4 0.0 0.0 4.0016214570143824E-4 0.0 26-27 1.600648582805753E-4 0.0 0.0 4.8019457484172585E-4 0.0 28-29 1.600648582805753E-4 0.0 0.0 7.803161841178045E-4 0.0 30-31 1.600648582805753E-4 0.0 0.0 0.002260916123213126 0.0 32-33 1.600648582805753E-4 0.0 0.0 0.006782748369639378 0.0 34-35 1.600648582805753E-4 0.0 0.0 0.013065294057151958 0.0 36-37 1.600648582805753E-4 0.0 0.0 0.022048934228149245 0.0 38-39 1.600648582805753E-4 0.0 0.0 0.03911584974231559 0.0 40-41 1.600648582805753E-4 0.0 0.0 0.058943884061821855 0.0 42-43 1.600648582805753E-4 0.0 0.0 0.07705122115481193 0.0 44-45 1.600648582805753E-4 0.0 0.0 0.09978043103065362 0.0 46-47 1.600648582805753E-4 0.0 0.0 0.12997266492382714 0.0 48-49 1.600648582805753E-4 0.0 0.0 0.16424655270315533 0.0 50-51 1.600648582805753E-4 0.0 0.0 0.20098143767854734 0.0 52-53 1.600648582805753E-4 0.0 0.0 0.23807646858507067 0.0 54-55 1.600648582805753E-4 0.0 0.0 0.27805266694064434 0.0 56-57 1.600648582805753E-4 0.0 0.0 0.32649229467780344 0.0 58-59 1.600648582805753E-4 0.0 0.0 0.3818947437501675 0.0 60-61 2.2008918013579102E-4 0.0 0.0 0.4452204033074202 0.0 62-63 3.201297165611506E-4 0.0 0.0 0.5148686247667555 0.0 64-65 3.201297165611506E-4 0.0 0.0 0.5814355977041897 0.0 66-67 3.601459311312944E-4 0.0 0.0 0.6638089753968308 0.0 68-69 3.601459311312944E-4 0.0 0.0 0.7570267472379808 0.0 70-71 4.0016214570143824E-4 0.0 0.0 0.8608288078329339 0.0 72-73 4.4017836027158204E-4 0.0 0.0 0.9730742897021873 0.0 74-75 4.4017836027158204E-4 0.0 0.0 1.0889012227754686 0.0 76-77 4.4017836027158204E-4 0.0 0.0 1.2159527040356752 0.0 78-79 4.4017836027158204E-4 0.0 0.0 1.3624120493624017 0.0 80-81 4.4017836027158204E-4 0.0 0.0 1.5298598992311683 0.0 82-83 4.4017836027158204E-4 0.0 0.0 1.7067515757384892 0.0 84-85 4.4017836027158204E-4 0.0 0.0 1.8806220280457642 0.0 86-87 4.4017836027158204E-4 0.0 0.0 2.0718395093691964 0.0 88-89 4.4017836027158204E-4 0.0 0.0 2.289267611236073 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3065 0.0 54.564777 1 GTATCAA 5845 0.0 39.91142 1 ATCAACG 6935 0.0 33.019817 3 TCAACGC 6935 0.0 33.019817 4 CAACGCA 7120 0.0 32.11939 5 AACGCAG 7425 0.0 31.118263 6 TATCAAC 7375 0.0 31.051065 2 ACGCAGA 8580 0.0 26.486895 7 CGCAGAG 8680 0.0 26.181747 8 GTACATG 6000 0.0 23.3599 1 GCAGAGT 10075 0.0 22.179853 9 ACATGGG 6050 0.0 21.516384 3 TACATGG 6345 0.0 21.265629 2 AGAGTAC 9410 0.0 19.688574 10-11 GAGTACT 7035 0.0 19.658813 12-13 CAGAGTA 10040 0.0 18.713037 10-11 GTACTTT 7635 0.0 18.020706 14-15 AGTACTT 7825 0.0 17.795353 12-13 ATTATAC 1820 0.0 16.445356 3 CATGGGT 1875 0.0 16.21634 4 >>END_MODULE