FastQCFastQC Report
Sat 18 Jun 2016
SRR1931000_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931000_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287954
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1488654.527587673063552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.052.9031521
GTATCAA51950.046.3896451
ATCAACG61950.038.488173
TCAACGC62750.038.0725634
CAACGCA63650.037.1263475
TATCAAC65100.035.912182
AACGCAG70300.034.47266
ACGCAGA78350.029.6307967
CGCAGAG79050.029.2060538
GTACATG55450.026.724221
GCAGAGT86700.025.0864189
TACATGG58800.024.7627582
ACATGGG57600.023.9792463
GTGTAGC10250.023.0113811
GAGTACT53250.021.82623912-13
AGAGTAC76000.020.69117510-11
CAGAGTA84600.020.07592610-11
GTGGTAT14150.019.5679841
GTACTTT61350.019.26577814-15
AGTACTT60500.018.81378212-13
Produced by FastQC (version 0.11.5)