##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931000_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3287954 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.848190090250654 28.0 2.0 31.0 2.0 33.0 2 19.5803423648871 27.0 2.0 31.0 2.0 33.0 3 20.21020032518703 27.0 2.0 31.0 2.0 33.0 4 21.725709970394963 30.0 2.0 35.0 2.0 37.0 5 21.085957102806184 30.0 2.0 35.0 2.0 37.0 6 22.041270650380145 30.0 2.0 35.0 2.0 37.0 7 22.207500165756578 30.0 2.0 35.0 2.0 37.0 8 22.627509995577796 30.0 2.0 35.0 2.0 37.0 9 23.864804677924326 32.0 2.0 37.0 2.0 39.0 10-11 24.213030504684674 32.0 2.0 37.0 2.0 39.0 12-13 24.112531379696918 32.0 2.0 37.0 2.0 39.0 14-15 24.064494819574726 32.0 2.0 38.0 2.0 40.0 16-17 22.93293960316963 30.5 2.0 38.0 2.0 40.0 18-19 21.912556258390477 28.0 2.0 37.0 2.0 40.0 20-21 21.587330449270276 27.0 2.0 37.0 2.0 40.0 22-23 21.213161589243647 27.0 2.0 37.0 2.0 39.5 24-25 21.051272767198082 27.0 2.0 37.0 2.0 39.0 26-27 20.767686074683525 26.5 2.0 37.0 2.0 39.0 28-29 20.56842948532735 25.0 2.0 36.0 2.0 39.0 30-31 20.535255511482216 25.0 2.0 36.0 2.0 39.0 32-33 20.393729960942274 25.0 2.0 36.0 2.0 39.0 34-35 20.06345815665304 24.5 2.0 36.0 2.0 39.0 36-37 19.613513297327152 24.0 2.0 36.0 2.0 39.0 38-39 19.48627185781796 24.0 2.0 35.0 2.0 39.0 40-41 19.341817586255768 23.5 2.0 35.0 2.0 39.0 42-43 19.205045751856627 23.5 2.0 35.0 2.0 38.5 44-45 18.817565422143982 20.5 2.0 35.0 2.0 38.0 46-47 18.48056831087053 18.0 2.0 35.0 2.0 38.0 48-49 18.22690448224032 16.5 2.0 34.5 2.0 38.0 50-51 16.01304732365477 10.0 2.0 32.5 2.0 37.5 52-53 17.85329143899215 17.0 2.0 33.5 2.0 38.0 54-55 18.623198347665447 20.0 2.0 35.0 2.0 39.0 56-57 18.590833843782487 19.5 2.0 35.0 2.0 39.0 58-59 18.31599712161423 18.0 2.0 35.0 2.0 39.0 60-61 18.03390847317207 14.5 2.0 35.0 2.0 39.0 62-63 17.59840131583349 11.0 2.0 34.0 2.0 38.0 64-65 17.246033855704795 8.0 2.0 34.0 2.0 38.0 66-67 16.88779770033279 8.0 2.0 34.0 2.0 38.0 68-69 16.4498432763962 6.5 2.0 33.0 2.0 37.5 70-71 16.105159013781822 2.0 2.0 32.5 2.0 37.0 72-73 15.707712912041956 2.0 2.0 32.0 2.0 36.0 74-75 15.264679189550705 2.0 2.0 31.5 2.0 36.0 76-77 14.853000224455695 2.0 2.0 31.0 2.0 35.5 78-79 14.471776217063864 2.0 2.0 31.0 2.0 35.0 80-81 14.035531823133779 2.0 2.0 30.0 2.0 34.5 82-83 13.586152817223113 2.0 2.0 30.0 2.0 34.0 84-85 13.185251223101051 2.0 2.0 29.0 2.0 34.0 86-87 12.703193992373372 2.0 2.0 29.0 2.0 34.0 88-89 12.283565706819498 2.0 2.0 29.0 2.0 34.0 90-91 11.797231348127134 2.0 2.0 27.5 2.0 34.0 92-93 11.25882250785747 2.0 2.0 26.0 2.0 34.0 94-95 10.700795844467411 2.0 2.0 25.5 2.0 33.0 96-97 10.071329921282352 2.0 2.0 24.5 2.0 33.0 98-99 9.408839661382125 2.0 2.0 19.0 2.0 32.0 100-101 8.506197319062249 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.954329007385915 1101 2 9.640730442315355 1101 3 8.809814961789804 1101 4 10.915581602709704 1101 5 11.579443348380966 1101 6 10.92550376646684 1101 7 10.397945622384274 1101 8 9.953328385058501 1101 9 9.915300551968695 1101 10-11 9.514764029418568 1101 12-13 9.347455677223493 1101 14-15 9.896246539014893 1101 16-17 10.937693609118991 1101 18-19 11.973813354409916 1101 20-21 11.800986000433081 1101 22-23 11.99109968791398 1101 24-25 11.93613644434171 1101 26-27 12.103433060590017 1101 28-29 12.05827491337471 1101 30-31 11.558068985824203 1101 32-33 11.39309473796487 1101 34-35 11.292701288342187 1101 36-37 11.727040957719726 1101 38-39 11.36006448793125 1101 40-41 10.816296308115302 1101 42-43 10.655780649504393 1101 44-45 10.718809687247372 1101 46-47 10.368668394582407 1101 48-49 10.708791342797667 1101 50-51 12.08497186226826 1101 52-53 11.32858401097306 1101 54-55 11.056635541103304 1101 56-57 11.063880343760527 1101 58-59 10.736401519750046 1101 60-61 10.719346932820553 1101 62-63 10.72468404814494 1101 64-65 10.45225577910108 1101 66-67 10.103996495802242 1101 68-69 10.09008154154115 1101 70-71 9.972857596252277 1101 72-73 9.714250663335132 1101 74-75 9.35117073107935 1101 76-77 9.192131240303587 1101 78-79 8.876124502168013 1101 80-81 8.720131244463282 1101 82-83 8.457246498401966 1101 84-85 8.238842861720348 1101 86-87 7.935888939261302 1101 88-89 7.761302789899785 1101 90-91 7.3956620971622815 1101 92-93 7.136349317056627 1101 94-95 6.872351455730289 1101 96-97 6.687200106739427 1101 98-99 6.295646249446252 1101 100-101 5.555226546735549 1102 1 7.709369903551661 1102 2 8.725025870027867 1102 3 8.229016928044775 1102 4 10.168054423551023 1102 5 10.910784621877372 1102 6 10.002915566599437 1102 7 9.72071363090404 1102 8 9.347028177631469 1102 9 9.086863997961391 1102 10-11 8.466409681656923 1102 12-13 8.575503043464138 1102 14-15 8.94153042087186 1102 16-17 9.696285671944292 1102 18-19 10.926616927013345 1102 20-21 10.916275781922547 1102 22-23 10.958448728536851 1102 24-25 10.73494103672844 1102 26-27 11.009035144837629 1102 28-29 10.76990538635027 1102 30-31 10.705943617910538 1102 32-33 10.689723388995102 1102 34-35 10.435989471291528 1102 36-37 10.559336719094048 1102 38-39 10.351662391826526 1102 40-41 10.06167452263779 1102 42-43 9.634684131227463 1102 44-45 9.720447540186019 1102 46-47 9.534632476593085 1102 48-49 9.138674056874436 1102 50-51 11.016941589417486 1102 52-53 10.182434668933912 1102 54-55 10.010832097554328 1102 56-57 9.860575645816649 1102 58-59 9.854925389764372 1102 60-61 9.686383052525255 1102 62-63 9.576380454574672 1102 64-65 9.315764057745927 1102 66-67 9.11842497432606 1102 68-69 8.99304627858455 1102 70-71 8.742739122811107 1102 72-73 8.743547743089344 1102 74-75 8.287729528219476 1102 76-77 8.105060012001543 1102 78-79 7.835644148450344 1102 80-81 7.540449206458369 1102 82-83 7.451985495695796 1102 84-85 7.376791868217898 1102 86-87 7.070479050363817 1102 88-89 6.779432501562477 1102 90-91 6.431119222884464 1102 92-93 6.125445887027645 1102 94-95 5.9344169362286046 1102 96-97 5.752936228685408 1102 98-99 5.256045508521817 1102 100-101 4.6274834887449074 1103 1 7.705064944210886 1103 2 9.127962063988573 1103 3 8.524057976669294 1103 4 10.262431391222055 1103 5 11.23238634216192 1103 6 10.4295388603553 1103 7 9.930426056009303 1103 8 9.512572555333684 1103 9 9.438119617002403 1103 10-11 8.78198256497964 1103 12-13 8.674523082003816 1103 14-15 9.35931971575025 1103 16-17 10.313651834029326 1103 18-19 10.98465272426714 1103 20-21 11.496467021802875 1103 22-23 11.68222223018639 1103 24-25 11.570983053004166 1103 26-27 11.154627136926504 1103 28-29 10.961703298935277 1103 30-31 10.896465547768482 1103 32-33 11.14272362989016 1103 34-35 11.006697525770981 1103 36-37 11.154131093654541 1103 38-39 10.807079548263001 1103 40-41 10.635297920645815 1103 42-43 10.276174137091424 1103 44-45 10.15529432048459 1103 46-47 10.023426741060458 1103 48-49 10.102833394177612 1103 50-51 11.63355687929779 1103 52-53 10.801288415542398 1103 54-55 10.472839840179994 1103 56-57 10.181210776883209 1103 58-59 10.150470220582939 1103 60-61 10.0968894312884 1103 62-63 9.974672117428494 1103 64-65 9.584342624669887 1103 66-67 9.512808774142087 1103 68-69 9.288211181961916 1103 70-71 8.865670783838306 1103 72-73 8.72622533505205 1103 74-75 8.64890489964656 1103 76-77 8.424120253997534 1103 78-79 7.993345675045353 1103 80-81 7.693289644190006 1103 82-83 7.370383975419637 1103 84-85 7.146103518989799 1103 86-87 7.032555999432303 1103 88-89 6.595065210403538 1103 90-91 6.21960804976092 1103 92-93 5.927720646163557 1103 94-95 5.528760008672528 1103 96-97 5.112037221063318 1103 98-99 4.797680750566485 1103 100-101 4.388686303801043 1104 1 7.904407323193091 1104 2 9.511256980336192 1104 3 8.840173637674493 1104 4 10.727456521678246 1104 5 11.355947292203826 1104 6 10.74474203574006 1104 7 10.475658818334981 1104 8 9.904990011714606 1104 9 9.558718870509043 1104 10-11 9.058238894774956 1104 12-13 8.940520961420518 1104 14-15 9.39198870831019 1104 16-17 10.337585859182948 1104 18-19 11.239128124385697 1104 20-21 11.547452809948712 1104 22-23 11.571626734023944 1104 24-25 11.420051437526308 1104 26-27 11.683774391363041 1104 28-29 11.273649208620043 1104 30-31 11.231663155764249 1104 32-33 11.068777723636753 1104 34-35 10.915072671711204 1104 36-37 11.05721387446669 1104 38-39 10.696556325216587 1104 40-41 10.330855032706463 1104 42-43 9.95810585808374 1104 44-45 10.108816187284166 1104 46-47 9.999614658024871 1104 48-49 9.861820824231387 1104 50-51 11.563879461880012 1104 52-53 10.730085370549489 1104 54-55 10.351153916409473 1104 56-57 10.229585553951743 1104 58-59 10.149557348933989 1104 60-61 9.87674650637609 1104 62-63 9.847884054802659 1104 64-65 9.581680271800362 1104 66-67 9.375576924559383 1104 68-69 9.249977759956401 1104 70-71 8.874522288963478 1104 72-73 8.785165626735193 1104 74-75 8.602319450519545 1104 76-77 8.412289895514304 1104 78-79 8.058984290592692 1104 80-81 8.064292511952935 1104 82-83 7.6536029299343245 1104 84-85 7.160805715148902 1104 86-87 6.983932577109387 1104 88-89 6.43293923986352 1104 90-91 6.148465654136592 1104 92-93 6.003457510256645 1104 94-95 5.852312551871265 1104 96-97 5.3223291701384365 1104 98-99 4.888220264016146 1104 100-101 4.336173729994536 1105 1 7.894825322924493 1105 2 8.940992785628623 1105 3 8.502986685622147 1105 4 10.554028031893633 1105 5 10.44393596580808 1105 6 10.311640275990023 1105 7 9.934852624295981 1105 8 9.35073029200441 1105 9 9.34427955680361 1105 10-11 8.756947061435977 1105 12-13 8.550875082823644 1105 14-15 9.177504042163154 1105 16-17 10.158029267159531 1105 18-19 10.487003232798532 1105 20-21 10.861111593072419 1105 22-23 11.276995099189755 1105 24-25 11.296792831126513 1105 26-27 11.346734039823044 1105 28-29 11.184781099293886 1105 30-31 10.648387203310676 1105 32-33 10.62446186145295 1105 34-35 10.772128093315281 1105 36-37 10.802435697693419 1105 38-39 10.737219182110803 1105 40-41 10.385694041998274 1105 42-43 10.079322474984053 1105 44-45 9.840875803152333 1105 46-47 9.753292243166339 1105 48-49 9.677391564208357 1105 50-51 11.179191935600487 1105 52-53 10.146255189503712 1105 54-55 9.865322454282733 1105 56-57 9.798569546297056 1105 58-59 9.874769373690398 1105 60-61 9.674591637829142 1105 62-63 9.764636803248482 1105 64-65 9.58561371751069 1105 66-67 9.334488616577815 1105 68-69 9.250143731483934 1105 70-71 9.11287334280109 1105 72-73 8.890364296787396 1105 74-75 8.663868612942785 1105 76-77 8.256676296643262 1105 78-79 8.04370248415976 1105 80-81 7.645048224205919 1105 82-83 7.648046921133274 1105 84-85 7.176067104615504 1105 86-87 7.046630988811655 1105 88-89 6.668636590134566 1105 90-91 6.382865313742252 1105 92-93 6.182268715656761 1105 94-95 5.798071031710975 1105 96-97 5.486665541332274 1105 98-99 4.936155410024652 1105 100-101 4.330696125691242 1106 1 7.740185824951386 1106 2 8.90601646316593 1106 3 8.605579823769727 1106 4 10.259968032567734 1106 5 10.964992092540108 1106 6 10.246635223142363 1106 7 9.394353282205483 1106 8 8.85438579106204 1106 9 8.635570696643722 1106 10-11 8.297707223713413 1106 12-13 8.103779097599507 1106 14-15 8.699315508131608 1106 16-17 9.94277741854108 1106 18-19 10.944828496771837 1106 20-21 10.992314333129467 1106 22-23 11.02216313504853 1106 24-25 10.905395557883764 1106 26-27 11.014223207234824 1106 28-29 10.902536887957567 1106 30-31 10.634566714677991 1106 32-33 10.75497162061642 1106 34-35 10.650962769092065 1106 36-37 10.74398000992106 1106 38-39 10.315254519995715 1106 40-41 10.381182502847796 1106 42-43 9.99408251254481 1106 44-45 9.502900375651937 1106 46-47 9.438954933888255 1106 48-49 9.599870868981565 1106 50-51 11.190431343688138 1106 52-53 10.662420699642574 1106 54-55 10.11528475357148 1106 56-57 9.953458560454717 1106 58-59 9.779198008542053 1106 60-61 9.642205339737544 1106 62-63 9.628299354678585 1106 64-65 9.469234582430015 1106 66-67 9.28144340528862 1106 68-69 9.043924153432119 1106 70-71 8.63035347115162 1106 72-73 8.353896591328457 1106 74-75 8.208617638831768 1106 76-77 8.151646255739335 1106 78-79 7.9155474770539715 1106 80-81 7.654955954561821 1106 82-83 7.379479724553194 1106 84-85 7.042960488991584 1106 86-87 6.783664532051619 1106 88-89 6.312914665612011 1106 90-91 6.012285706609751 1106 92-93 5.889470582531388 1106 94-95 5.692876068310682 1106 96-97 4.966258007616327 1106 98-99 4.616455448888887 1106 100-101 4.116745686578367 1107 1 7.06834379737078 1107 2 8.001338373160639 1107 3 8.001998140360712 1107 4 9.827657743174822 1107 5 10.696312307748595 1107 6 9.706894226529212 1107 7 9.460277645483256 1107 8 8.143305684864892 1107 9 5.47050835007083 1107 10-11 7.6259734090744296 1107 12-13 7.561516376537256 1107 14-15 8.03680268155761 1107 16-17 9.027655501238694 1107 18-19 10.423493226602805 1107 20-21 10.66406835521563 1107 22-23 9.995010347035294 1107 24-25 10.140370516732741 1107 26-27 10.211790057387862 1107 28-29 10.135792440489794 1107 30-31 9.953018802779493 1107 32-33 10.352394610241419 1107 34-35 10.590352304587189 1107 36-37 10.79605645708564 1107 38-39 10.397877286629477 1107 40-41 9.978521775905914 1107 42-43 9.923783154473547 1107 44-45 9.403049976744558 1107 46-47 9.489211102458942 1107 48-49 9.502050122564654 1107 50-51 11.057730976333245 1107 52-53 10.172754911848646 1107 54-55 9.993981551383925 1107 56-57 9.414230049445823 1107 58-59 9.701252647334758 1107 60-61 9.462150593226774 1107 62-63 9.661939599269527 1107 64-65 9.562021777473863 1107 66-67 9.622752260612621 1107 68-69 9.231213990826504 1107 70-71 9.063063414362096 1107 72-73 8.61757849707713 1107 74-75 8.641851119882915 1107 76-77 8.487958345492073 1107 78-79 8.123447590900257 1107 80-81 7.892251924297824 1107 82-83 7.710475358672371 1107 84-85 7.525418775253248 1107 86-87 7.02307226249714 1107 88-89 6.932506503221996 1107 90-91 6.637825833114972 1107 92-93 6.335673409380043 1107 94-95 6.3101886295980165 1107 96-97 5.896970052896183 1107 98-99 5.509119922868829 1107 100-101 4.918160009930348 1108 1 6.221591475041194 1108 2 7.617569285075856 1108 3 7.397456421225112 1108 4 8.620191439661141 1108 5 10.391577684184103 1108 6 8.999966679587278 1108 7 8.254060463024832 1108 8 5.699312270720135 1108 9 6.671320913121082 1108 10-11 5.8066839548048925 1108 12-13 4.554011393275822 1108 14-15 6.76823345737284 1108 16-17 7.953791147283166 1108 18-19 7.713806235973681 1108 20-21 7.83899996538474 1108 22-23 8.628620081973978 1108 24-25 8.263966975332025 1108 26-27 6.685855719040166 1108 28-29 8.551775703157414 1108 30-31 8.85140129728956 1108 32-33 9.38726537086486 1108 34-35 9.039406586814234 1108 36-37 8.931097383883703 1108 38-39 8.5141609661239 1108 40-41 8.821951264867767 1108 42-43 7.774810515760926 1108 44-45 7.412519223873549 1108 46-47 8.248601115834145 1108 48-49 8.593463712873216 1108 50-51 10.040748836984823 1108 52-53 9.41744274898343 1108 54-55 8.983654553043571 1108 56-57 8.76968749822193 1108 58-59 8.68348694444877 1108 60-61 8.767007764447886 1108 62-63 8.720839010093346 1108 64-65 8.53634633392343 1108 66-67 7.997538420100874 1108 68-69 8.28295085155511 1108 70-71 8.090706252409293 1108 72-73 7.845915840499327 1108 74-75 8.001456940711236 1108 76-77 7.5856305579036185 1108 78-79 7.35981470990165 1108 80-81 7.375548616911644 1108 82-83 7.24339338932535 1108 84-85 6.894966362258996 1108 86-87 6.442369790494643 1108 88-89 6.393245776594716 1108 90-91 6.267602771664427 1108 92-93 5.933237079581438 1108 94-95 5.819490182101756 1108 96-97 5.433457948277146 1108 98-99 5.13702618688715 1108 100-101 4.764079214157357 1109 1 6.433715135538179 1109 2 7.8446383029919 1109 3 7.781216838895556 1109 4 8.99106520208019 1109 5 10.058286355095667 1109 6 8.821895119178997 1109 7 7.355097226622469 1109 8 8.003255480981029 1109 9 7.29748066267695 1109 10-11 5.757202583687505 1109 12-13 6.122989528830935 1109 14-15 7.513523916726317 1109 16-17 6.244059752075106 1109 18-19 5.361868481322947 1109 20-21 7.511504975008652 1109 22-23 8.697959083409536 1109 24-25 8.299860096766299 1109 26-27 8.714457397727461 1109 28-29 9.047864737331572 1109 30-31 6.5983169502949615 1109 32-33 5.721851789657062 1109 34-35 7.294177404241548 1109 36-37 5.621133235742143 1109 38-39 6.086229872852236 1109 40-41 6.907393211295062 1109 42-43 5.881931223606578 1109 44-45 6.295106487692326 1109 46-47 6.059232143134434 1109 48-49 6.900294097261472 1109 50-51 8.525663506596727 1109 52-53 8.737288642821408 1109 54-55 8.725590479274235 1109 56-57 7.931708248318916 1109 58-59 7.759456563025516 1109 60-61 7.99157666499913 1109 62-63 8.372344862272996 1109 64-65 8.182321462468774 1109 66-67 7.941612856564511 1109 68-69 7.631483791531043 1109 70-71 7.143676693775072 1109 72-73 6.621576233708474 1109 74-75 5.69504919315181 1109 76-77 5.490939770917029 1109 78-79 6.264522891587307 1109 80-81 6.234582247486777 1109 82-83 6.054139310589628 1109 84-85 5.715389320296756 1109 86-87 5.766287378166153 1109 88-89 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-13.886403904958376 2313 72-73 -13.494284445623705 2313 74-75 -13.04804234398118 2313 76-77 -12.654569728219416 2313 78-79 -12.28095782228719 2313 80-81 -11.85220531728006 2313 82-83 -11.400955680774791 2313 84-85 -11.016907743606525 2313 86-87 -10.54761590335371 2313 88-89 -10.122777113247915 2313 90-91 -9.654428701869195 2313 92-93 -9.119552619989378 2313 94-95 -8.553042006739446 2313 96-97 -7.942793839991809 2313 98-99 -7.284862225130018 2313 100-101 -6.40058459127898 2314 1 -18.631627409078977 2314 2 -17.374705393035732 2314 3 -17.99224314959034 2314 4 -19.494830019566326 2314 5 -18.80584720851976 2314 6 -19.78000470810943 2314 7 -19.936497476889333 2314 8 -20.391102607677578 2314 9 -21.627677656263753 2314 10-11 -21.983873985109273 2314 12-13 -21.88075255522203 2314 14-15 -21.833626342341038 2314 16-17 -20.683977093060182 2314 18-19 -19.6608718756143 2314 20-21 -19.341114483828424 2314 22-23 -18.96107948884147 2314 24-25 -18.79282844669945 2314 26-27 -18.521869602848245 2314 28-29 -18.32504833117735 2314 30-31 -18.281216728461512 2314 32-33 -18.15336409980753 2314 34-35 -17.821251496161445 2314 36-37 -17.387214490222163 2314 38-39 -17.265238175507005 2314 40-41 -17.12211168219947 2314 42-43 -16.96982467737212 2314 44-45 -16.592630990718728 2314 46-47 -16.257693590889748 2314 48-49 -15.998381780689016 2314 50-51 -13.844658888337067 2314 52-53 -15.63706368878481 2314 54-55 -16.396602957686042 2314 56-57 -16.36650706544044 2314 58-59 -16.098344242961815 2314 60-61 -15.811676069600754 2314 62-63 -15.390176726673463 2314 64-65 -15.01687255019674 2314 66-67 -14.655537402502844 2314 68-69 -14.231498361606551 2314 70-71 -13.886403904958376 2314 72-73 -13.494284445623705 2314 74-75 -13.04804234398118 2314 76-77 -12.654569728219416 2314 78-79 -12.28095782228719 2314 80-81 -11.85220531728006 2314 82-83 -11.400955680774791 2314 84-85 -11.016907743606525 2314 86-87 -10.54761590335371 2314 88-89 -10.122777113247915 2314 90-91 -9.654428701869195 2314 92-93 -9.119552619989378 2314 94-95 -8.553042006739446 2314 96-97 -7.942793839991809 2314 98-99 -7.284862225130018 2314 100-101 -6.40058459127898 2315 1 -18.631627409078977 2315 2 -17.374705393035732 2315 3 -17.99224314959034 2315 4 -19.494830019566326 2315 5 -18.80584720851976 2315 6 -19.78000470810943 2315 7 -19.936497476889333 2315 8 -20.391102607677578 2315 9 -21.627677656263753 2315 10-11 -21.983873985109273 2315 12-13 -21.88075255522203 2315 14-15 -21.833626342341038 2315 16-17 -20.683977093060182 2315 18-19 -19.6608718756143 2315 20-21 -19.341114483828424 2315 22-23 -18.96107948884147 2315 24-25 -18.79282844669945 2315 26-27 -18.521869602848245 2315 28-29 -18.32504833117735 2315 30-31 -18.281216728461512 2315 32-33 -18.15336409980753 2315 34-35 -17.821251496161445 2315 36-37 -17.387214490222163 2315 38-39 -17.265238175507005 2315 40-41 -17.12211168219947 2315 42-43 -16.96982467737212 2315 44-45 -16.592630990718728 2315 46-47 -16.257693590889748 2315 48-49 -15.998381780689016 2315 50-51 -13.844658888337067 2315 52-53 -15.63706368878481 2315 54-55 -16.396602957686042 2315 56-57 -16.36650706544044 2315 58-59 -16.098344242961815 2315 60-61 -15.811676069600754 2315 62-63 -15.390176726673463 2315 64-65 -15.01687255019674 2315 66-67 -14.655537402502844 2315 68-69 -14.231498361606551 2315 70-71 -13.886403904958376 2315 72-73 -13.494284445623705 2315 74-75 -13.04804234398118 2315 76-77 -12.654569728219416 2315 78-79 -12.28095782228719 2315 80-81 -11.85220531728006 2315 82-83 -11.400955680774791 2315 84-85 -11.016907743606525 2315 86-87 -10.54761590335371 2315 88-89 -10.122777113247915 2315 90-91 -9.654428701869195 2315 92-93 -9.119552619989378 2315 94-95 -8.553042006739446 2315 96-97 -7.942793839991809 2315 98-99 -7.284862225130018 2315 100-101 -6.40058459127898 2316 1 -18.631627409078977 2316 2 -17.374705393035732 2316 3 -17.99224314959034 2316 4 -19.494830019566326 2316 5 -18.80584720851976 2316 6 -19.78000470810943 2316 7 -19.936497476889333 2316 8 -20.391102607677578 2316 9 -21.627677656263753 2316 10-11 -21.983873985109273 2316 12-13 -21.88075255522203 2316 14-15 -21.833626342341038 2316 16-17 -20.683977093060182 2316 18-19 -19.6608718756143 2316 20-21 -19.341114483828424 2316 22-23 -18.96107948884147 2316 24-25 -18.79282844669945 2316 26-27 -18.521869602848245 2316 28-29 -18.32504833117735 2316 30-31 -18.281216728461512 2316 32-33 -18.15336409980753 2316 34-35 -17.821251496161445 2316 36-37 -17.387214490222163 2316 38-39 -17.265238175507005 2316 40-41 -17.12211168219947 2316 42-43 -16.96982467737212 2316 44-45 -16.592630990718728 2316 46-47 -16.257693590889748 2316 48-49 -15.998381780689016 2316 50-51 -13.844658888337067 2316 52-53 -15.63706368878481 2316 54-55 -16.396602957686042 2316 56-57 -16.36650706544044 2316 58-59 -16.098344242961815 2316 60-61 -15.811676069600754 2316 62-63 -15.390176726673463 2316 64-65 -15.01687255019674 2316 66-67 -14.655537402502844 2316 68-69 -14.231498361606551 2316 70-71 -13.886403904958376 2316 72-73 -13.494284445623705 2316 74-75 -13.04804234398118 2316 76-77 -12.654569728219416 2316 78-79 -12.28095782228719 2316 80-81 -11.85220531728006 2316 82-83 -11.400955680774791 2316 84-85 -11.016907743606525 2316 86-87 -10.54761590335371 2316 88-89 -10.122777113247915 2316 90-91 -9.654428701869195 2316 92-93 -9.119552619989378 2316 94-95 -8.553042006739446 2316 96-97 -7.942793839991809 2316 98-99 -7.284862225130018 2316 100-101 -6.40058459127898 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 867157.0 3 34441.0 4 126760.0 5 67254.0 6 40620.0 7 40311.0 8 39016.0 9 38301.0 10 40446.0 11 42666.0 12 44127.0 13 45148.0 14 46321.0 15 47805.0 16 49632.0 17 49632.0 18 49118.0 19 50651.0 20 52871.0 21 56011.0 22 59940.0 23 63691.0 24 67676.0 25 72645.0 26 77182.0 27 82835.0 28 89210.0 29 95295.0 30 102931.0 31 111459.0 32 120463.0 33 127769.0 34 131374.0 35 122553.0 36 90605.0 37 38993.0 38 5005.0 39 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.500114524855434 21.34896528855745 14.44840075763201 22.702519428955107 2 14.473131935417916 21.063743058368278 42.403141354467046 22.05998365174676 3 18.059276930089688 27.08172853192635 30.876948195819388 23.982046342164573 4 11.778738747475867 17.87978026621593 40.83882610066052 29.502654885647683 5 12.064637718208049 38.58986178457176 36.42294265514675 12.92255784207344 6 28.315264029197067 39.88383523275595 18.58293510678973 13.21796563125725 7 25.073943283801825 33.74271478145652 23.98948201286631 17.193859921875347 8 23.899269724595484 37.32037611569854 21.133311059137988 17.64704310056799 9 24.93963588448568 17.206420015211275 22.319982062047874 35.533962038255176 10-11 23.328177435583928 27.810359978196818 29.704398606084055 19.1570639801352 12-13 24.086478923219744 25.4131031584364 29.80784067572319 20.69257724262066 14-15 21.475291174388634 27.088149639213178 27.47294075057273 23.963618435825463 16-17 20.578200030570535 30.105111036559585 28.301528725387243 21.015160207482637 18-19 20.369452576578446 28.908102510134214 30.54840793577113 20.17403697751621 20-21 21.19764972238693 28.154859576303163 30.562126030941727 20.085364670368175 22-23 20.868466795272813 28.04601941102102 30.279477096989694 20.80603669671647 24-25 20.70844389188785 28.30385070223183 30.49480477138496 20.492900634495363 26-27 20.668243528765377 28.465975178779136 30.274509434172508 20.591271858282983 28-29 20.6301898412898 28.50616592331284 30.41464496406284 20.44899927133452 30-31 20.876041210434742 28.307301446987548 30.305236159526906 20.51142118305081 32-33 20.74226777541953 28.613675621375773 30.073275253491953 20.570781349712743 34-35 20.86341030202575 28.49838763824944 30.22581973702487 20.41238232269994 36-37 20.984703390903032 28.345679741484776 30.1134589910819 20.556157876530293 38-39 20.852097708556048 28.606626299844084 30.149326257167363 20.391949734432504 40-41 20.930110381920876 28.47627593979197 30.242208173917344 20.351405504369797 42-43 20.94986730498183 28.463902276049964 29.870190369945337 20.71604004902287 44-45 20.87104931002971 28.785623668588478 29.7518703515884 20.591456669793413 46-47 21.03344752608048 28.680163477265136 29.43120282534754 20.85518617130684 48-49 20.96443505728272 28.81861512493768 29.383820493328166 20.83312932445143 50-51 21.194350538209346 29.01930261985039 28.876952663777782 20.909394178162476 52-53 20.721448576120334 29.20632891186971 29.05541268802267 21.016809823987288 54-55 20.754843131027798 29.08827595758246 29.150359727691033 21.006521183698712 56-57 20.753752951544012 29.227199546380824 29.135022327848347 20.884025174226817 58-59 20.74167117804853 29.09421590155658 29.24794757470139 20.916165345693503 60-61 20.752403735241668 29.316734945689415 29.111384097357902 20.819477221711015 62-63 20.635550938351546 29.20509989707799 29.182742156745856 20.976607007824605 64-65 20.489409882847344 29.03878113284636 29.197536268577963 21.274272715728333 66-67 20.498023632240553 29.07352016597876 29.034332280316065 21.39412392146462 68-69 20.599498262504618 29.16620738429504 29.012874120692167 21.22142023250818 70-71 20.684314488667845 28.88971175986469 29.074147904616897 21.351825846850566 72-73 20.65442280985177 29.044480029727882 28.996790238925264 21.304306921495087 74-75 20.52952786055603 29.36755304532963 28.741145176351562 21.36177391776278 76-77 20.53928362275309 29.231698796045897 28.86607681114077 21.36294077006024 78-79 20.46399845853601 29.286635692939132 28.783783365498756 21.4655824830261 80-81 20.53339993546285 29.23095712984553 28.63787013290497 21.597772801786654 82-83 20.67729364096636 29.05387279156848 28.904808310969365 21.364025256495793 84-85 20.734963824268718 29.12801180071913 28.65946392739919 21.47756044761296 86-87 20.59984498602113 29.18229542802097 28.75869036910516 21.459169216852732 88-89 20.727407461901937 29.09076174667911 28.60450662951502 21.57732416190393 90-91 20.660489415410655 29.02601874400729 28.781149710454713 21.532342130127347 92-93 20.663536301836345 29.039331644762857 28.53375149255834 21.763380560842457 94-95 20.400740659272742 28.970338120132755 28.70753344607907 21.921387774515434 96-97 20.542611146918198 28.83285605980852 28.712187023562745 21.91234576971054 98-99 20.4125175536685 28.929047183772223 28.37785588388818 22.280579378671092 100-101 20.57277483977219 29.13413463549733 28.284339067721508 22.008751457008977 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 202044.0 1 183165.0 2 121752.5 3 61877.0 4 38146.5 5 30143.0 6 28384.0 7 28467.5 8 28812.5 9 28334.0 10 25938.5 11 21875.5 12 17361.5 13 13183.5 14 10191.0 15 8545.5 16 8121.5 17 8851.5 18 10172.5 19 11748.5 20 13520.0 21 15533.0 22 17633.5 23 20040.0 24 22536.5 25 25693.0 26 29941.5 27 34728.5 28 40285.5 29 47025.0 30 53905.5 31 60860.0 32 68502.0 33 75292.5 34 81262.5 35 86334.5 36 91568.5 37 96337.0 38 98001.5 39 97565.5 40 96255.0 41 94584.0 42 93053.0 43 92061.5 44 91406.0 45 90621.5 46 88743.0 47 86178.5 48 83339.5 49 79761.0 50 75413.0 51 69886.5 52 63891.5 53 57977.0 54 51545.5 55 44574.0 56 37636.5 57 31035.5 58 25188.5 59 20146.5 60 15566.0 61 11565.0 62 8358.5 63 5821.5 64 3897.5 65 2513.0 66 1601.5 67 1000.0 68 594.5 69 351.0 70 219.0 71 150.5 72 102.5 73 71.0 74 44.0 75 34.0 76 31.0 77 20.0 78 15.0 79 14.5 80 8.0 81 4.0 82 2.5 83 2.5 84 4.0 85 2.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.587146900473666 2 29.640226110219302 3 23.402699672805642 4 29.752545199841602 5 32.89851378699337 6 25.465593496746003 7 24.38984851977856 8 20.34940269845624 9 12.104548907922679 10-11 15.77374561809563 12-13 15.835592590407288 14-15 20.01899965753779 16-17 23.39415332452948 18-19 27.40275563465912 20-21 29.47361793991035 22-23 29.09245384819861 24-25 27.56417820930585 26-27 29.034104491729508 28-29 25.975196125006615 30-31 27.885289757703426 32-33 26.867483547519218 34-35 24.89678991859375 36-37 27.90470912914232 38-39 28.02119494372488 40-41 25.029760148712544 42-43 25.898780822359434 44-45 24.56659977603093 46-47 27.91518676964459 48-49 23.284890847013067 50-51 28.841568343109426 52-53 22.45150023388405 54-55 23.57758046493351 56-57 25.711323820223765 58-59 24.999406926009303 60-61 24.524293831361387 62-63 24.36528917375365 64-65 22.880125451876758 66-67 23.398091944108707 68-69 24.397908243241844 70-71 22.874924649189133 72-73 22.73687527258593 74-75 19.97476242064214 76-77 22.280801373741845 78-79 21.393182508027788 80-81 22.42990625781261 82-83 23.30405169902012 84-85 20.22725987042398 86-87 21.793887627381647 88-89 20.624071991274818 90-91 21.763032572840128 92-93 21.739096714856718 94-95 20.77211238356741 96-97 19.685798523945287 98-99 19.096906465236437 100-101 20.755916901513828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3287954.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.19586939664194 #Duplication Level Percentage of deduplicated Percentage of total 1 86.3669425896108 61.48969564797202 2 8.501845255797779 12.10592528924477 3 2.3555625480557745 5.03118970581 4 1.0135638717329334 2.8864624414821067 5 0.5044562862561155 1.7957601936302712 6 0.2971519556332349 1.2693595094532317 7 0.20056870045359812 0.9995764101784 8 0.13958746523306534 0.7950440755313293 9 0.10582656674491141 0.678097298219915 >10 0.4838397542308968 6.013894037398995 >50 0.02187472671702492 1.0426844154174788 >100 0.008387026947104428 0.9767091761073444 >500 2.1847365957853156E-4 0.09709070294046769 >1k 1.310841954823404E-4 0.18749300402402896 >5k 0.0 0.0 >10k+ 4.369473182744679E-5 4.6310180925895255 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 148865 4.527587673063552 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.0828101609694055E-5 0.0 0.0 0.0 0.0 7 6.0828101609694055E-5 0.0 0.0 3.0414050804847028E-5 0.0 8 6.0828101609694055E-5 0.0 0.0 3.0414050804847028E-5 0.0 9 6.0828101609694055E-5 0.0 0.0 3.0414050804847028E-5 0.0 10-11 6.0828101609694055E-5 0.0 0.0 6.0828101609694055E-5 0.0 12-13 6.0828101609694055E-5 0.0 0.0 6.0828101609694055E-5 0.0 14-15 6.0828101609694055E-5 0.0 0.0 6.0828101609694055E-5 0.0 16-17 6.0828101609694055E-5 0.0 0.0 6.0828101609694055E-5 0.0 18-19 6.0828101609694055E-5 0.0 0.0 6.0828101609694055E-5 0.0 20-21 9.124215241454108E-5 0.0 0.0 1.064491778169646E-4 0.0 22-23 9.124215241454108E-5 0.0 0.0 2.2810538103635268E-4 0.0 24-25 9.124215241454108E-5 0.0 0.0 2.889334826460467E-4 0.0 26-27 9.124215241454108E-5 0.0 0.0 3.649686096581643E-4 0.0 28-29 9.124215241454108E-5 0.0 0.0 6.23488041499364E-4 0.0 30-31 9.124215241454108E-5 0.0 0.0 0.001566323616449622 0.0 32-33 9.124215241454108E-5 0.0 0.0 0.004060275782447078 0.0 34-35 9.124215241454108E-5 0.0 0.0 0.007192923015346322 0.0 36-37 9.124215241454108E-5 0.0 0.0 0.013123662922291491 0.0 38-39 9.124215241454108E-5 0.0 0.0 0.022019772782709245 0.0 40-41 9.124215241454108E-5 0.0 0.0 0.03131126530359002 0.0 42-43 9.124215241454108E-5 0.0 0.0 0.04099814048493379 0.0 44-45 1.2165620321938811E-4 0.0 0.0 0.05366559264515258 0.0 46-47 1.5207025402423513E-4 0.0 0.0 0.06961776229229484 0.0 48-49 1.5207025402423513E-4 0.0 0.0 0.08757725929255701 0.0 50-51 1.5207025402423513E-4 0.0 0.0 0.10900395808457175 0.0 52-53 1.5207025402423513E-4 0.0 0.0 0.13482548721788687 0.0 54-55 1.5207025402423513E-4 0.0 0.0 0.16346031605065034 0.0 56-57 1.5207025402423513E-4 0.0 0.0 0.19773695130771296 0.0 58-59 1.5207025402423513E-4 0.0 0.0 0.23684942064274622 0.0 60-61 1.6727727942665864E-4 0.0 0.0 0.2799005095570072 0.0 62-63 1.8248430482908215E-4 0.0 0.0 0.32462437126553473 0.0 64-65 1.8248430482908215E-4 0.0 0.0 0.3716597008352307 0.0 66-67 1.8248430482908215E-4 0.0 0.0 0.4283362845100631 0.0 68-69 1.8248430482908215E-4 0.0 0.0 0.49439560285819084 0.0 70-71 1.8248430482908215E-4 0.0 0.0 0.5701113823368575 0.0 72-73 1.8248430482908215E-4 0.0 0.0 0.6444585295293062 0.0 74-75 1.8248430482908215E-4 0.0 0.0 0.7210867305321182 0.0 76-77 1.8248430482908215E-4 0.0 0.0 0.8098501378060642 0.0 78-79 1.8248430482908215E-4 0.0 0.0 0.9122694538913866 0.0 80-81 1.8248430482908215E-4 0.0 0.0 1.0263069373841605 0.0 82-83 1.8248430482908215E-4 0.0 0.0 1.1466401293935378 0.0 84-85 1.8248430482908215E-4 0.0 0.0 1.2673382900125731 0.0 86-87 1.8248430482908215E-4 0.0 0.0 1.3996698250644626 0.0 88-89 1.8248430482908215E-4 0.0 0.0 1.5494134042021268 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2520 0.0 52.903152 1 GTATCAA 5195 0.0 46.389645 1 ATCAACG 6195 0.0 38.48817 3 TCAACGC 6275 0.0 38.072563 4 CAACGCA 6365 0.0 37.126347 5 TATCAAC 6510 0.0 35.91218 2 AACGCAG 7030 0.0 34.4726 6 ACGCAGA 7835 0.0 29.630796 7 CGCAGAG 7905 0.0 29.206053 8 GTACATG 5545 0.0 26.72422 1 GCAGAGT 8670 0.0 25.086418 9 TACATGG 5880 0.0 24.762758 2 ACATGGG 5760 0.0 23.979246 3 GTGTAGC 1025 0.0 23.011381 1 GAGTACT 5325 0.0 21.826239 12-13 AGAGTAC 7600 0.0 20.691175 10-11 CAGAGTA 8460 0.0 20.075926 10-11 GTGGTAT 1415 0.0 19.567984 1 GTACTTT 6135 0.0 19.265778 14-15 AGTACTT 6050 0.0 18.813782 12-13 >>END_MODULE