FastQCFastQC Report
Sat 18 Jun 2016
SRR1931000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287954
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91750.27904891613447147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81970.2493039744473311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46840.14245941396990347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47100.059.6378751
GTATCAA93600.042.95861
ATCAACG115200.034.0766333
TCAACGC115750.033.9952774
CAACGCA116300.033.6524475
TATCAAC119550.032.997732
AACGCAG121050.032.3304526
ACGCAGA136850.028.389767
CGCAGAG137250.028.3070228
GCAGAGT156250.024.2880469
GTGGTAT24850.023.9077761
GTACATG88650.022.7322651
TGGTATC24950.022.6689822
ACATGGG84450.022.2856143
GAGTACT110900.022.09334212-13
TACATGG90000.021.9159932
ACGTGCG1954.2802276E-721.8942729
AGAGTAC143750.021.1695410-11
CAGAGTA152600.021.1541810-11
TACCGTG5000.020.872547
Produced by FastQC (version 0.11.5)