##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931000_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3287954 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.350742741534706 31.0 31.0 34.0 30.0 34.0 2 31.508957242102536 31.0 31.0 34.0 30.0 34.0 3 31.57114424350219 31.0 31.0 34.0 30.0 34.0 4 35.34557539430296 37.0 35.0 37.0 33.0 37.0 5 35.221381746824925 37.0 35.0 37.0 33.0 37.0 6 35.24807585507583 37.0 35.0 37.0 32.0 37.0 7 35.13067427342353 37.0 35.0 37.0 32.0 37.0 8 35.13684102636472 37.0 35.0 37.0 32.0 37.0 9 36.63269619952104 39.0 35.0 39.0 32.0 39.0 10-11 36.57752754448511 38.5 35.0 39.0 32.0 39.0 12-13 36.463992349041376 38.0 35.0 39.0 32.0 39.0 14-15 37.52261406333544 39.0 36.0 41.0 32.0 41.0 16-17 37.48275401663162 39.0 36.0 41.0 32.0 41.0 18-19 37.5200865036433 39.0 36.0 41.0 32.0 41.0 20-21 37.511257152624395 39.0 36.0 41.0 32.0 41.0 22-23 37.4119204526584 39.0 36.0 41.0 32.0 41.0 24-25 37.28766384809519 39.0 36.0 41.0 31.5 41.0 26-27 37.03344633167009 39.0 36.0 40.0 31.0 41.0 28-29 36.84566572403385 39.0 36.0 40.0 30.5 41.0 30-31 36.62696391129559 38.5 35.0 40.0 30.0 41.0 32-33 36.45085287081267 38.0 35.0 40.0 30.0 41.0 34-35 36.512552334977926 38.0 35.0 40.0 30.0 41.0 36-37 36.57663610865602 39.0 35.0 40.0 30.0 41.0 38-39 36.562166928126125 39.0 35.0 40.0 30.0 41.0 40-41 36.44943192635907 39.0 35.0 40.0 30.0 41.0 42-43 36.301500416368356 39.0 35.0 40.0 30.0 41.0 44-45 36.125369910892914 38.0 35.0 40.0 30.0 41.0 46-47 35.8800988395823 38.0 35.0 40.0 29.0 41.0 48-49 35.65174740887494 38.0 35.0 40.0 28.0 41.0 50-51 35.383858624542796 38.0 34.0 40.0 27.0 41.0 52-53 35.17960287765583 38.0 34.0 40.0 26.5 41.0 54-55 35.030267455079965 38.0 34.0 40.0 26.0 41.0 56-57 34.86211516341166 38.0 34.0 40.0 26.0 41.0 58-59 34.664865293127576 38.0 33.5 40.0 26.0 41.0 60-61 34.367335279021546 37.5 33.0 40.0 25.5 41.0 62-63 34.12086209235287 37.0 33.0 40.0 25.0 41.0 64-65 33.816346731128235 37.0 32.5 40.0 24.0 41.0 66-67 33.50817240752151 36.0 32.0 39.0 23.0 41.0 68-69 33.175266746432584 36.0 32.0 39.0 22.5 40.0 70-71 32.80793222776231 35.5 31.0 39.0 22.0 40.0 72-73 32.39710941819746 35.0 31.0 38.0 22.0 40.0 74-75 31.88671465598363 35.0 31.0 37.5 20.0 39.5 76-77 30.497717729627603 33.5 29.5 36.0 19.5 39.0 78-79 31.23876657033523 34.0 30.0 36.0 20.0 39.0 80-81 31.207677479672768 34.0 31.0 36.0 20.0 39.0 82-83 30.94078171409941 34.0 30.5 36.0 20.0 37.5 84-85 30.590780467123324 34.0 30.0 35.0 18.5 37.0 86-87 30.24081054661957 34.0 30.0 35.0 18.0 36.5 88-89 29.893483455060505 34.0 30.0 35.0 15.0 36.0 90-91 29.555390677606802 34.0 29.0 35.0 9.0 36.0 92-93 29.23423046672794 34.0 29.0 35.0 4.5 35.5 94-95 28.851358778133758 34.0 29.0 35.0 2.0 35.0 96-97 28.43006851677365 34.0 29.0 35.0 2.0 35.0 98-99 27.94589872607707 34.0 29.0 35.0 2.0 35.0 100-101 26.817544740589437 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31274291165251356 1101 2 0.4127950683544519 1101 3 0.33561269727195153 1101 4 0.26112072661256747 1101 5 0.283993837510117 1101 6 0.34215694542231034 1101 7 0.40010573927013127 1101 8 0.35220407281153854 1101 9 0.4986878822525469 1101 10-11 0.44891839436528613 1101 12-13 0.46239073823141297 1101 14-15 0.5096136485542999 1101 16-17 0.6248247024578077 1101 18-19 0.6101696695340024 1101 20-21 0.5402228397493403 1101 22-23 0.6334407955570569 1101 24-25 0.6208492064379101 1101 26-27 0.5112712624103253 1101 28-29 0.6224259798151124 1101 30-31 0.6646559466947082 1101 32-33 0.7011763081227045 1101 34-35 0.5957298256761092 1101 36-37 0.7207094287334357 1101 38-39 0.7251257911868976 1101 40-41 0.7590580040172554 1101 42-43 0.6892837187339467 1101 44-45 0.6839917497015406 1101 46-47 0.7540886693624174 1101 48-49 0.710927669913751 1101 50-51 0.6890810503365046 1101 52-53 0.570368198273485 1101 54-55 0.6155484310110069 1101 56-57 0.8516249560890188 1101 58-59 0.7199513734421714 1101 60-61 0.7460492252987478 1101 62-63 0.6138136513904371 1101 64-65 0.6242668023159155 1101 66-67 0.7054723471913746 1101 68-69 0.5740459900267396 1101 70-71 0.5752693764423 1101 72-73 0.6180416843901639 1101 74-75 0.7657046935022578 1101 76-77 0.6542909373735419 1101 78-79 0.5669607838909378 1101 80-81 0.49899355542573076 1101 82-83 0.5944332555627803 1101 84-85 0.5830020324743614 1101 86-87 0.6040973268410141 1101 88-89 0.7395859833972587 1101 90-91 0.5485005270011385 1101 92-93 0.515389020291142 1101 94-95 0.5304826969899636 1101 96-97 0.8156520773727642 1101 98-99 0.8833469116271964 1101 100-101 0.9727868387390224 1102 1 0.28790751935225956 1102 2 0.40323717568004724 1102 3 0.32238045780471225 1102 4 0.19906306348881486 1102 5 0.28147610065541784 1102 6 0.30401376076309106 1102 7 0.3403039083596724 1102 8 0.3350127172191435 1102 9 0.4905977581361398 1102 10-11 0.3640295239930893 1102 12-13 0.3845843640255353 1102 14-15 0.447751284233199 1102 16-17 0.45825940560001044 1102 18-19 0.47379570585578534 1102 20-21 0.4938941409291928 1102 22-23 0.5312167389379354 1102 24-25 0.4950289243545001 1102 26-27 0.509478191658836 1102 28-29 0.3682671817350993 1102 30-31 0.48180818830201133 1102 32-33 0.7086535591108571 1102 34-35 0.6140812254289827 1102 36-37 0.5493527138888439 1102 38-39 0.5507273494562526 1102 40-41 0.7007608595550607 1102 42-43 0.685828981423036 1102 44-45 0.7545914986779607 1102 46-47 0.7018245498283449 1102 48-49 0.614678392004187 1102 50-51 0.5258192494447584 1102 52-53 0.579276164367954 1102 54-55 0.7371375252222094 1102 56-57 0.6191670090454551 1102 58-59 0.6789494246113605 1102 60-61 0.6143985374059397 1102 62-63 0.5293172764832477 1102 64-65 0.5089499537318645 1102 66-67 0.40451088241962196 1102 68-69 0.47249675809960223 1102 70-71 0.583180333563682 1102 72-73 0.5489494234644567 1102 74-75 0.5820101281772025 1102 76-77 0.5513170906034794 1102 78-79 0.5991789860646897 1102 80-81 0.4189145845790243 1102 82-83 0.36027572995251944 1102 84-85 0.3966209563409038 1102 86-87 0.39634897111052325 1102 88-89 0.4046081395538792 1102 90-91 0.3935169190866681 1102 92-93 0.524123603344016 1102 94-95 0.5087036270207577 1102 96-97 0.6874902882658311 1102 98-99 0.8486111985344209 1102 100-101 0.9728382617000157 1103 1 0.3230033277959059 1103 2 0.3731411344832729 1103 3 0.35911244107551354 1103 4 0.24460107986224244 1103 5 0.27839393596992323 1103 6 0.3128023595060796 1103 7 0.35973596241220207 1103 8 0.32892033776889207 1103 9 0.5393151114127264 1103 10-11 0.37824396980258257 1103 12-13 0.4264943521082145 1103 14-15 0.572069065350739 1103 16-17 0.5065812555607963 1103 18-19 0.5710130581588757 1103 20-21 0.48581437722936727 1103 22-23 0.4868499663848169 1103 24-25 0.5249620383629932 1103 26-27 0.6029010485114199 1103 28-29 0.5443620462453467 1103 30-31 0.5180940024149194 1103 32-33 0.5683164777147027 1103 34-35 0.5044410068923852 1103 36-37 0.6074172291614346 1103 38-39 0.5687059354606632 1103 40-41 0.6582604042262261 1103 42-43 0.6139205226080335 1103 44-45 0.6277327702675777 1103 46-47 0.665372489803886 1103 48-49 0.5252185332455142 1103 50-51 0.46657256068984765 1103 52-53 0.490399117667053 1103 54-55 0.5499759575599938 1103 56-57 0.5866732566526522 1103 58-59 0.5738861237259982 1103 60-61 0.48144163284197106 1103 62-63 0.38761668656470505 1103 64-65 0.39551603623833387 1103 66-67 0.36779602376094545 1103 68-69 0.4289910836137878 1103 70-71 0.47372202531252583 1103 72-73 0.34928848193469264 1103 74-75 0.47380904080415576 1103 76-77 0.4836220386163461 1103 78-79 0.589093518762791 1103 80-81 0.5754085383962 1103 82-83 0.5468566980391429 1103 84-85 0.6326080676586727 1103 86-87 0.39829184021848363 1103 88-89 0.44128290803531556 1103 90-91 0.4930911401646121 1103 92-93 0.3838283821108419 1103 94-95 0.5777515676157883 1103 96-97 0.6949251576576962 1103 98-99 0.6126125823092785 1103 100-101 0.8225665600374832 1104 1 0.35474173428735867 1104 2 0.4195651807998324 1104 3 0.40557551916393564 1104 4 0.14852064181424396 1104 5 0.2662596872629024 1104 6 0.32410278662518266 1104 7 0.31208691894796914 1104 8 0.36974611280691505 1104 9 0.5082124509900012 1104 10-11 0.4467526644514592 1104 12-13 0.43919340342170443 1104 14-15 0.5768801815993712 1104 16-17 0.6047545686376381 1104 18-19 0.6072741747077544 1104 20-21 0.49317426818060284 1104 22-23 0.41244961878759057 1104 24-25 0.3444980835609286 1104 26-27 0.3966402665205919 1104 28-29 0.5944353058786831 1104 30-31 0.5531818925289045 1104 32-33 0.553276249254445 1104 34-35 0.47593747644717865 1104 36-37 0.47178889912935773 1104 38-39 0.5730362746424689 1104 40-41 0.5200368902858727 1104 42-43 0.6314830613717319 1104 44-45 0.6801340391861928 1104 46-47 0.5542661063506102 1104 48-49 0.5222757092153216 1104 50-51 0.4002479295829815 1104 52-53 0.23922010080447365 1104 54-55 0.46510613066375583 1104 56-57 0.6156336303968857 1104 58-59 0.5425585802484605 1104 60-61 0.4735737712597867 1104 62-63 0.4213535626675622 1104 64-65 0.44841785546203994 1104 66-67 0.37809873874237354 1104 68-69 0.37587686292386735 1104 70-71 0.4917579637092402 1104 72-73 0.47787518007841356 1104 74-75 0.4828961519983892 1104 76-77 0.500605320150374 1104 78-79 0.6007939572053935 1104 80-81 0.5635064643936794 1104 82-83 0.43944868219996636 1104 84-85 0.5204053203366357 1104 86-87 0.5494992516227981 1104 88-89 0.6882435768768964 1104 90-91 0.5214315092250281 1104 92-93 0.48079226785669604 1104 94-95 0.6683109400878351 1104 96-97 0.6133604639970578 1104 98-99 0.6643739875216959 1104 100-101 0.7145138336896686 1105 1 0.2487107381527771 1105 2 0.2751094540290495 1105 3 0.28216893159627787 1105 4 0.051401651483438116 1105 5 0.1145039745915426 1105 6 0.19914877709698686 1105 7 0.21618725662283822 1105 8 0.22519917909637144 1105 9 0.29211216686783104 1105 10-11 0.3209887540334009 1105 12-13 0.32253721718549855 1105 14-15 0.3864249041691963 1105 16-17 0.34130851105761195 1105 18-19 0.3157297692703125 1105 20-21 0.2859008076010312 1105 22-23 0.3795684710528491 1105 24-25 0.35593526645470064 1105 26-27 0.36594490564861815 1105 28-29 0.35413995437843226 1105 30-31 0.19608534412584078 1105 32-33 0.3270727046515347 1105 34-35 0.45878350114249855 1105 36-37 0.3191135025360623 1105 38-39 0.301870135344366 1105 40-41 0.2373480929665206 1105 42-43 0.3595427090368162 1105 44-45 0.47873317589995423 1105 46-47 0.516784739970241 1105 48-49 0.4801929108859966 1105 50-51 0.47122178122276637 1105 52-53 0.2876216155090816 1105 54-55 0.35933302424324864 1105 56-57 0.454960807680159 1105 58-59 0.5315288580823747 1105 60-61 0.3614858394022491 1105 62-63 0.1825609082569315 1105 64-65 0.2582143175532181 1105 66-67 0.33259584752421745 1105 68-69 0.21324037152931652 1105 70-71 0.15523171807556224 1105 72-73 0.3127000815811982 1105 74-75 0.34980137920198473 1105 76-77 0.4011946090249232 1105 78-79 0.5067751660655198 1105 80-81 0.46408562846115586 1105 82-83 0.5246314454271683 1105 84-85 0.515837568014522 1105 86-87 0.453516666290966 1105 88-89 0.4911819883448736 1105 90-91 0.4755750446821416 1105 92-93 0.3792647608059383 1105 94-95 0.4092986275693491 1105 96-97 0.35706967451731586 1105 98-99 0.4656851918758633 1105 100-101 0.5029123946282432 1106 1 0.24765180570938838 1106 2 0.23249808538267303 1106 3 0.24780683466988052 1106 4 0.11889838802208885 1106 5 0.1839603199835551 1106 6 0.19063292967236833 1106 7 0.2624013309090927 1106 8 0.31220082683069705 1106 9 0.2729165629902184 1106 10-11 0.31422436447313373 1106 12-13 0.2608422909269308 1106 14-15 0.33051431460460634 1106 16-17 0.3491432198768578 1106 18-19 0.3531725173966791 1106 20-21 0.29620202489716974 1106 22-23 0.31946107392072065 1106 24-25 0.4149795904832203 1106 26-27 0.3018028906095722 1106 28-29 0.41636753516706193 1106 30-31 0.3497236718655543 1106 32-33 0.5182024746817504 1106 34-35 0.4096182541011899 1106 36-37 0.427406839162046 1106 38-39 0.4589792472895198 1106 40-41 0.3486289987508542 1106 42-43 0.3650049437726679 1106 44-45 0.44716060035381844 1106 46-47 0.28633931838519544 1106 48-49 0.3561114024865404 1106 50-51 0.27005227658899145 1106 52-53 0.30590229910558264 1106 54-55 0.35788110173885457 1106 56-57 0.4223063190264398 1106 58-59 0.3927830773116199 1106 60-61 0.3488503663165048 1106 62-63 0.18521660349903613 1106 64-65 0.1568892565964859 1106 66-67 0.1615274106093736 1106 68-69 0.12192767541213101 1106 70-71 0.05924620409250991 1106 72-73 0.19398346099593766 1106 74-75 0.2980283857638568 1106 76-77 0.23141172288703515 1106 78-79 0.20265617806606784 1106 80-81 0.14383587046204482 1106 82-83 0.21825836481986016 1106 84-85 0.31439022508032366 1106 86-87 0.283090238742453 1106 88-89 0.29402292016235876 1106 90-91 0.31402457777715753 1106 92-93 0.3867926052966517 1106 94-95 0.4157150360451496 1106 96-97 0.6295195681810206 1106 98-99 0.6381978496671081 1106 100-101 0.803782123743769 1107 1 0.12283114680309382 1107 2 0.2264970509263371 1107 3 0.20045451749840737 1107 4 0.017018872740187874 1107 5 0.070563302124377 1107 6 0.1329217802010163 1107 7 0.1480425210436067 1107 8 0.1949850138516922 1107 9 0.24440336014530573 1107 10-11 0.1684404998640261 1107 12-13 0.30190233896457386 1107 14-15 0.27216333137207727 1107 16-17 0.2682530974997377 1107 18-19 0.2955740759929597 1107 20-21 0.314563542395625 1107 22-23 0.21505212236802862 1107 24-25 0.17836663246183804 1107 26-27 0.18758796601593275 1107 28-29 0.20397538829241313 1107 30-31 0.2940006877584267 1107 32-33 0.29435852005299523 1107 34-35 0.37105403198851405 1107 36-37 0.3813861102802534 1107 38-39 0.3118538556258912 1107 40-41 0.267030131093712 1107 42-43 0.25388802702990176 1107 44-45 0.22630301774020722 1107 46-47 0.3105528301071274 1107 48-49 0.10778109435926098 1107 50-51 0.16543465221442233 1107 52-53 0.06542043974330625 1107 54-55 0.13458171719170764 1107 56-57 0.25803132057911427 1107 58-59 0.2219616495325809 1107 60-61 0.2127172485622637 1107 62-63 0.03050211946898429 1107 64-65 -0.01864611497268953 1107 66-67 -0.016020251541576158 1107 68-69 -0.035084354122425054 1107 70-71 -0.06300716050930788 1107 72-73 0.012705875253054444 1107 74-75 0.1915956377564072 1107 76-77 0.21256114392717862 1107 78-79 0.2656515749745587 1107 80-81 0.25382174751844744 1107 82-83 0.23282970754203802 1107 84-85 0.2620265847276855 1107 86-87 0.3361341689903057 1107 88-89 0.48939181865771175 1107 90-91 0.4114318550809841 1107 92-93 0.2489821869472344 1107 94-95 0.49176151581299976 1107 96-97 0.4908311568715469 1107 98-99 0.5966543075259594 1107 100-101 0.8230130563471469 1108 1 0.044695337636365906 1108 2 0.12894326692245173 1108 3 0.10010087997501671 1108 4 0.009652007896448822 1108 5 -0.03423533456899008 1108 6 0.0010243346708733725 1108 7 0.05252488180165926 1108 8 0.019566302492819432 1108 9 0.09135727851909081 1108 10-11 0.029249470929180177 1108 12-13 0.047880819184143775 1108 14-15 0.026995189808324938 1108 16-17 0.06452591865389223 1108 18-19 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-1.0397219051683102 2316 92-93 -0.9787175851262511 2316 94-95 -1.3458573383113404 2316 96-97 -1.0012492713181445 2316 98-99 -1.0533508322573049 2316 100-101 -1.4123165759834357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 3.0 4 39.0 5 66.0 6 135.0 7 454.0 8 1086.0 9 2108.0 10 3765.0 11 6221.0 12 8674.0 13 11483.0 14 14028.0 15 17938.0 16 21778.0 17 25506.0 18 21989.0 19 16480.0 20 15637.0 21 16478.0 22 18007.0 23 21368.0 24 26306.0 25 33522.0 26 43651.0 27 55836.0 28 71462.0 29 89258.0 30 109514.0 31 132943.0 32 160459.0 33 196029.0 34 241595.0 35 302533.0 36 398868.0 37 549403.0 38 542868.0 39 110440.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.614693956009866 22.49482028865257 15.275282040301377 22.615203715036188 2 14.50659162407653 22.17494547154529 41.342469111694896 21.975993792683287 3 17.755302715327243 27.451335825913937 31.710814014211543 23.08254744454728 4 12.00934855637255 18.69415742647198 40.495664821776 28.800829195379468 5 11.58800228231341 39.18282595657035 36.81659859873743 12.412573162378813 6 28.056124048831926 41.029328170207954 18.153391905122096 12.761155875838027 7 24.638589614446925 34.17057183316545 24.014523326374597 17.17631522601303 8 23.699402450963845 38.099309261660295 20.866435457435088 17.334852829940775 9 24.378382422625133 16.997409331152443 22.28464875116866 36.33955949505376 10-11 23.074486443545133 28.139414359203325 29.74778540089065 19.0383137963609 12-13 23.7280843953413 25.597636098315245 30.08238558082017 20.591893925523287 14-15 21.01455798955825 27.344162965783585 27.714393206230987 23.926885838427182 16-17 20.03709601776667 30.540360357839557 28.549821560763927 20.872722063629844 18-19 19.888584207686606 29.231400439300547 31.046298092978187 19.83371726003466 20-21 20.635887850012498 28.387988396431336 31.11723886648049 19.85888488707567 22-23 20.33422401138462 28.171430509243695 31.013023486254106 20.481321993117586 24-25 20.292615051228925 28.493485657802193 31.054528864979947 20.159370425988936 26-27 20.235925459732858 28.58915394981022 30.95090257022766 20.224018020229266 28-29 20.187922651464888 28.599246612287267 31.006872522486283 20.20595821376156 30-31 20.516520752854138 28.319689414743337 30.914033807840273 20.249756024562245 32-33 20.426998900294926 28.61566448500188 30.658393274611417 20.29894334009178 34-35 20.495353748712162 28.514452824024662 30.867358035266427 20.122835391996748 36-37 20.59706081924668 28.34386632533669 30.876224899427392 20.182847955989235 38-39 20.35571105943081 28.676246396120135 30.83091756731665 20.1371249771324 40-41 20.454372989879385 28.534455267483573 30.85387346031737 20.15729828231967 42-43 20.509903941501044 28.558772714276152 30.492383455307554 20.43893988891525 44-45 20.48472801820106 28.80734977431168 30.37527526675021 20.332646940737046 46-47 20.57405818389098 28.73226181564668 30.104504456275393 20.58917554418695 48-49 20.627558943628223 28.880237217809952 29.94359278765146 20.548611050910363 50-51 20.681466683507374 29.042558491625076 29.56600183957823 20.709972985289316 52-53 20.689476150616404 29.14646218436475 29.492900861750034 20.67116080326881 54-55 20.70936114635954 28.997206134184957 29.65983098092843 20.633601738527073 56-57 20.67465643270963 29.198851189150016 29.4731879501087 20.653304428031653 58-59 20.62504420330249 29.103530408449924 29.656237095487498 20.615188292760088 60-61 20.663809867771548 29.263044549375095 29.588804279838797 20.48434130301456 62-63 20.612790059215328 29.172381749984684 29.650116476421335 20.564711714378657 64-65 20.40215630563294 29.01693634715289 29.611136908211883 20.96977043900229 66-67 20.452742589079467 29.05772416935928 29.553531230279965 20.93600201128129 68-69 20.501160483317214 29.174614625626738 29.521235076233328 20.80298981482272 70-71 20.60579703384812 28.863664113549902 29.56361047203164 20.966928380570337 72-73 20.658296232695445 29.031883167909346 29.4984152167221 20.811405382673108 74-75 20.53329568051297 29.297121770227424 29.323653590189814 20.845928959069788 76-77 20.444364525203515 29.221173451505443 29.409098805579852 20.925363217711187 78-79 20.364797804223628 29.24959847361423 29.370577259668533 21.015026462493612 80-81 20.423865612413806 29.19982876881585 29.32513471529774 21.051170903472602 82-83 20.535460654254894 29.181703880285433 29.438641781484776 20.8441936839749 84-85 20.61567771325268 29.15151793486162 29.23993158055131 20.99287277133439 86-87 20.379771128184885 29.24412871956238 29.327554460920073 21.048545691332667 88-89 20.4742133435403 29.110961372441587 29.24739894958881 21.167426334429305 90-91 20.47502091095734 29.00542924492434 29.50000760398449 21.01954224013383 92-93 20.507814906248655 29.098637363132102 29.237685976760236 21.15586175385901 94-95 20.117690265802988 28.97432758467304 29.404880214911337 21.503101934612634 96-97 20.234173091004205 28.866718725407374 29.469373417042977 21.42973476654544 98-99 20.114667054344434 28.967756239898733 29.3189320775169 21.59864462823993 100-101 20.084420280819014 28.94211719507025 29.292791200850132 21.680671323260604 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 227.0 1 162.5 2 172.0 3 257.5 4 234.5 5 245.0 6 425.0 7 910.5 8 1584.5 9 2048.0 10 2008.5 11 1846.0 12 2001.5 13 2295.5 14 2662.5 15 3123.5 16 3462.0 17 3845.5 18 4435.0 19 4986.0 20 5665.5 21 6553.5 22 7899.0 23 10159.5 24 13132.0 25 17213.5 26 23538.5 27 31299.0 28 40061.5 29 51304.0 30 62528.0 31 73327.5 32 85917.0 33 97775.5 34 107575.5 35 114873.0 36 125114.5 37 135379.0 38 136800.0 39 135147.0 40 133670.5 41 130473.5 42 127261.5 43 126935.5 44 127691.5 45 128640.5 46 126675.5 47 122604.5 48 120149.0 49 115809.0 50 108540.0 51 100041.5 52 90776.5 53 81709.5 54 72593.5 55 61536.0 56 50093.5 57 39689.0 58 31213.5 59 24187.5 60 17562.5 61 12059.0 62 8201.0 63 5184.5 64 2950.0 65 1681.5 66 911.0 67 471.5 68 237.5 69 129.5 70 69.5 71 35.5 72 24.5 73 20.5 74 13.5 75 9.5 76 8.0 77 5.5 78 2.5 79 0.5 80 0.5 81 0.5 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003832170401410725 2 0.006721505227871193 3 0.0019160852007053626 4 0.17445499541660253 5 0.0018856711499005156 6 6.995231685114816E-4 7 6.082810160969405E-5 8 6.995231685114816E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.889334826460467E-4 24-25 5.018318382799759E-4 26-27 0.002843713750253197 28-29 1.6727727942665867E-4 30-31 0.006858368456493004 32-33 0.021563562020636542 34-35 0.014142533624253868 36-37 9.428355749502578E-4 38-39 1.3686322862181162E-4 40-41 0.001840050073693245 42-43 0.011374855001012788 44-45 0.0071168878883342045 46-47 0.01043201942606253 48-49 0.010416812400660107 50-51 0.18928184518396549 52-53 0.11627291622693019 54-55 0.01744245813657977 56-57 0.006280501491200911 58-59 0.017837840797042783 60-61 0.12445429589343403 62-63 0.23999727490104789 64-65 0.24589760075718822 66-67 0.2570747644279695 68-69 0.25201082496896243 70-71 0.23861343558942735 72-73 0.1620612697136274 74-75 0.26984866576600525 76-77 0.20876204472446996 78-79 0.005702634525908818 80-81 4.562107620727054E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.0644917781696459E-4 90-91 0.006204466364188793 92-93 0.0186894342195785 94-95 0.0026308153946192677 96-97 4.562107620727054E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3287954.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.556951116082516 #Duplication Level Percentage of deduplicated Percentage of total 1 68.2056735759725 31.75448210516033 2 16.60065491749657 15.457517589776854 3 6.187638628901806 8.642327675092957 4 2.9689598585146766 5.529028759939163 5 1.625169886263253 3.7831477475043824 6 1.0025131529747826 2.800437351376603 7 0.6761341019978299 2.2035119634240528 8 0.4835778159678709 1.8011126991070492 9 0.35098985913563635 1.470691594261666 >10 1.6946187188727542 14.286069099685253 >50 0.12733837310338647 4.094554781119726 >100 0.07312246478764214 6.17880621748291 >500 0.0026244698274241786 0.7727736637883048 >1k 8.529526927065036E-4 0.6948792506700397 >5k 1.3122349118561594E-4 0.530659501610688 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9175 0.27904891613447147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8197 0.2493039744473311 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4684 0.14245941396990347 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.124215241454108E-5 0.0 0.0 0.0 0.0 7 9.124215241454108E-5 0.0 0.0 0.0 0.0 8 9.124215241454108E-5 0.0 0.0 0.0 0.0 9 9.124215241454108E-5 0.0 0.0 0.0 0.0 10-11 9.124215241454108E-5 0.0 0.0 0.0 0.0 12-13 9.124215241454108E-5 0.0 0.0 0.0 0.0 14-15 9.124215241454108E-5 0.0 0.0 0.0 0.0 16-17 2.1289835563392917E-4 0.0 0.0 0.0 0.0 18-19 2.1289835563392917E-4 0.0 0.0 0.0 0.0 20-21 2.1289835563392917E-4 0.0 0.0 1.5207025402423514E-5 0.0 22-23 2.2810538103635268E-4 0.0 0.0 7.603512701211757E-5 0.0 24-25 2.5851943184119976E-4 0.0 0.0 1.3686322862181162E-4 0.0 26-27 2.7372645724362324E-4 0.0 0.0 2.7372645724362324E-4 0.0 28-29 2.7372645724362324E-4 0.0 0.0 7.147301939139051E-4 0.0 30-31 2.7372645724362324E-4 0.0 0.0 0.0023570889373756444 0.0 32-33 2.7372645724362324E-4 0.0 0.0 0.006523813897639686 0.0 34-35 3.0414050804847026E-4 0.0 0.0 0.01272828026182848 0.0 36-37 3.0414050804847026E-4 0.0 0.0 0.02367733855157341 0.0 38-39 3.0414050804847026E-4 0.0 0.0 0.0421690814409204 0.0 40-41 3.0414050804847026E-4 0.0 0.0 0.06277460086120426 0.0 42-43 3.0414050804847026E-4 0.0 0.0 0.08658880264139948 0.0 44-45 3.193475334508938E-4 0.0 0.0 0.11400706944196909 0.0 46-47 3.345545588533173E-4 0.0 0.0 0.14750814640350807 0.0 48-49 3.345545588533173E-4 0.0 0.0 0.18528239750312808 0.0 50-51 3.345545588533173E-4 0.0 0.0 0.22763396324887758 0.0 52-53 3.345545588533173E-4 0.0 0.0 0.2729813129989045 0.0 54-55 3.345545588533173E-4 1.5207025402423514E-5 0.0 0.3239704691732305 0.0 56-57 3.345545588533173E-4 3.0414050804847028E-5 0.0 0.3838709422333767 0.0 58-59 3.345545588533173E-4 3.0414050804847028E-5 0.0 0.45391450123693944 0.0 60-61 3.4976158425574077E-4 3.0414050804847028E-5 0.0 0.5304514600873369 0.0 62-63 4.562107620727054E-4 3.0414050804847028E-5 0.0 0.6120979794729489 0.0 64-65 4.562107620727054E-4 3.0414050804847028E-5 0.0 0.6965577985580089 0.0 66-67 4.562107620727054E-4 3.0414050804847028E-5 0.0 0.7995853956594283 0.0 68-69 4.8662481287755244E-4 3.0414050804847028E-5 0.0 0.9192798926019038 0.0 70-71 5.018318382799759E-4 3.0414050804847028E-5 0.0 1.0559910509696913 0.0 72-73 5.778669652920934E-4 3.0414050804847028E-5 0.0 1.1962454462562433 6.0828101609694055E-5 74-75 6.082810160969405E-4 3.0414050804847028E-5 0.0 1.339617281750292 6.0828101609694055E-5 76-77 6.23488041499364E-4 3.0414050804847028E-5 0.0 1.5060429677544152 6.0828101609694055E-5 78-79 6.386950669017876E-4 3.0414050804847028E-5 0.0 1.6948838092017102 6.0828101609694055E-5 80-81 6.386950669017876E-4 3.0414050804847028E-5 0.0 1.908557723131163 6.0828101609694055E-5 82-83 6.539020923042111E-4 3.0414050804847028E-5 0.0 2.138153392656953 6.0828101609694055E-5 84-85 6.843161431090582E-4 3.0414050804847028E-5 0.0 2.370288635424948 6.0828101609694055E-5 86-87 6.995231685114816E-4 3.0414050804847028E-5 0.0 2.6260251816175044 7.603512701211757E-5 88-89 6.995231685114816E-4 3.0414050804847028E-5 0.0 2.9182890028266817 9.124215241454108E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4710 0.0 59.637875 1 GTATCAA 9360 0.0 42.9586 1 ATCAACG 11520 0.0 34.076633 3 TCAACGC 11575 0.0 33.995277 4 CAACGCA 11630 0.0 33.652447 5 TATCAAC 11955 0.0 32.99773 2 AACGCAG 12105 0.0 32.330452 6 ACGCAGA 13685 0.0 28.38976 7 CGCAGAG 13725 0.0 28.307022 8 GCAGAGT 15625 0.0 24.288046 9 GTGGTAT 2485 0.0 23.907776 1 GTACATG 8865 0.0 22.732265 1 TGGTATC 2495 0.0 22.668982 2 ACATGGG 8445 0.0 22.285614 3 GAGTACT 11090 0.0 22.093342 12-13 TACATGG 9000 0.0 21.915993 2 ACGTGCG 195 4.2802276E-7 21.894272 9 AGAGTAC 14375 0.0 21.16954 10-11 CAGAGTA 15260 0.0 21.15418 10-11 TACCGTG 500 0.0 20.87254 7 >>END_MODULE