FastQCFastQC Report
Sat 18 Jun 2016
SRR1930999_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930999_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059213
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1385604.529269455902547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.057.4958081
GTATCAA49150.046.86771
ATCAACG60350.037.5983773
TCAACGC61350.036.9818654
CAACGCA63350.036.0486345
TATCAAC62850.035.910522
AACGCAG68250.032.4920236
ACGCAGA78500.027.7157617
GTACATG55650.027.5649241
CGCAGAG80900.026.8287338
TACATGG57100.026.051712
ACATGGG56400.025.9270253
GTGGTAT14200.025.2906781
GCAGAGT86550.023.4070999
TGGTATC13650.022.5472162
GAGTACT53250.020.79929212-13
AGAGTAC76750.020.3053610-11
CATGGGG33250.020.1674444
CAGAGTA82950.019.39460610-11
GTATAGG9450.018.4585631
Produced by FastQC (version 0.11.5)