##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930999_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3059213 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.56035849743055 28.0 2.0 31.0 2.0 33.0 2 19.327613997456208 27.0 2.0 31.0 2.0 33.0 3 19.93641371163106 27.0 2.0 31.0 2.0 33.0 4 21.456199028965948 30.0 2.0 35.0 2.0 37.0 5 20.819652309270392 28.0 2.0 35.0 2.0 37.0 6 21.73847489534073 29.0 2.0 35.0 2.0 37.0 7 21.87047158860792 29.0 2.0 35.0 2.0 37.0 8 22.266692119836048 30.0 2.0 35.0 2.0 37.0 9 23.453988983441167 32.0 2.0 37.0 2.0 39.0 10-11 23.773840036636873 32.0 2.0 37.0 2.0 39.0 12-13 23.650611448107732 32.0 2.0 37.0 2.0 39.0 14-15 23.589051007563057 31.5 2.0 38.0 2.0 40.0 16-17 22.479838932431313 30.0 2.0 37.0 2.0 40.0 18-19 21.476778668239184 27.0 2.0 37.0 2.0 39.5 20-21 21.15158195915093 27.0 2.0 37.0 2.0 39.5 22-23 20.788568170964233 26.5 2.0 37.0 2.0 39.0 24-25 20.608270166215952 26.0 2.0 36.5 2.0 39.0 26-27 20.31498574960292 25.0 2.0 36.0 2.0 39.0 28-29 20.099820607456884 24.0 2.0 36.0 2.0 39.0 30-31 20.04116450864977 24.0 2.0 36.0 2.0 39.0 32-33 19.891309627672214 24.5 2.0 36.0 2.0 39.0 34-35 19.54951093630944 24.0 2.0 35.5 2.0 39.0 36-37 19.10992467670607 21.0 2.0 35.0 2.0 39.0 38-39 18.962249277837145 20.0 2.0 35.0 2.0 38.0 40-41 18.792508890358402 19.0 2.0 35.0 2.0 38.0 42-43 18.623704364488514 18.5 2.0 35.0 2.0 38.0 44-45 18.221000629900566 16.5 2.0 34.0 2.0 38.0 46-47 17.8703048790653 15.0 2.0 34.0 2.0 38.0 48-49 17.587629073228964 14.0 2.0 34.0 2.0 38.0 50-51 15.430224047818834 7.0 2.0 31.5 2.0 37.5 52-53 17.216301382087487 13.5 2.0 33.5 2.0 37.5 54-55 17.942395969159385 15.0 2.0 34.5 2.0 38.5 56-57 17.889630601072888 13.5 2.0 35.0 2.0 39.0 58-59 17.60861682400016 11.0 2.0 34.0 2.0 38.5 60-61 17.307786512413486 8.0 2.0 34.0 2.0 38.0 62-63 16.86609529967348 7.5 2.0 34.0 2.0 38.0 64-65 16.498332577692366 4.5 2.0 33.5 2.0 38.0 66-67 16.124150721116834 2.0 2.0 33.0 2.0 37.5 68-69 15.678745317831742 2.0 2.0 32.0 2.0 37.0 70-71 15.313804726901985 2.0 2.0 31.5 2.0 36.0 72-73 14.917479103285714 2.0 2.0 31.0 2.0 36.0 74-75 14.462926249332753 2.0 2.0 31.0 2.0 35.5 76-77 14.038229276614606 2.0 2.0 30.0 2.0 35.0 78-79 13.655893852438519 2.0 2.0 30.0 2.0 34.5 80-81 13.221008475055513 2.0 2.0 29.0 2.0 34.0 82-83 12.764118255250615 2.0 2.0 29.0 2.0 34.0 84-85 12.35343501743749 2.0 2.0 29.0 2.0 34.0 86-87 11.880687287874366 2.0 2.0 27.5 2.0 34.0 88-89 11.463176803968864 2.0 2.0 26.5 2.0 34.0 90-91 10.985976785532749 2.0 2.0 26.0 2.0 33.0 92-93 10.454547133527479 2.0 2.0 25.0 2.0 33.0 94-95 9.897730560114644 2.0 2.0 22.5 2.0 32.0 96-97 9.284784191228265 2.0 2.0 19.0 2.0 32.0 98-99 8.649247698672827 2.0 2.0 2.0 2.0 32.0 100-101 7.7990839474073885 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.865821670201424 1101 2 9.32478281665848 1101 3 8.693065727473698 1101 4 10.828430108356745 1101 5 11.519600398556808 1101 6 10.835258676547017 1101 7 10.467436192500543 1101 8 9.79365357074953 1101 9 9.855292048467117 1101 10-11 9.216520872929376 1101 12-13 9.167300297247682 1101 14-15 9.649166614659343 1101 16-17 10.760997242875934 1101 18-19 11.531614542181849 1101 20-21 11.424395877306726 1101 22-23 11.680422073520269 1101 24-25 11.2908006592873 1101 26-27 11.540481925283611 1101 28-29 11.305192658779067 1101 30-31 11.024318254149279 1101 32-33 10.837928928835929 1101 34-35 10.775471287743922 1101 36-37 11.042580639646491 1101 38-39 10.65413281052658 1101 40-41 10.553406633549795 1101 42-43 10.164978635094567 1101 44-45 10.214155716525212 1101 46-47 10.051552748499773 1101 48-49 10.243321632368595 1101 50-51 11.717387390040093 1101 52-53 11.067604147313578 1101 54-55 10.57239590505612 1101 56-57 10.564234912598476 1101 58-59 10.440427708760783 1101 60-61 10.003334953742439 1101 62-63 10.044885041910383 1101 64-65 9.91413557142744 1101 66-67 9.534358781912843 1101 68-69 9.65008898253174 1101 70-71 9.384392598678156 1101 72-73 9.314539648445477 1101 74-75 8.79860748126241 1101 76-77 8.723289866720501 1101 78-79 8.607328251185344 1101 80-81 8.233697772375505 1101 82-83 7.7588505079010215 1101 84-85 7.490222662079233 1101 86-87 7.356126000603117 1101 88-89 7.180965407200775 1101 90-91 6.760133489538729 1101 92-93 6.5531781367074124 1101 94-95 6.263356553025179 1101 96-97 6.006110240612257 1101 98-99 5.604160892285945 1101 100-101 4.903996651186694 1102 1 8.006587963933526 1102 2 9.120091309281495 1102 3 8.537532578806118 1102 4 10.412209037899515 1102 5 11.274028937386298 1102 6 10.321034245420538 1102 7 10.116935422210691 1102 8 9.511057754007545 1102 9 9.244867095224972 1102 10-11 8.549060842292473 1102 12-13 8.776174844272493 1102 14-15 9.191384339733013 1102 16-17 9.880295487532226 1102 18-19 10.905153147842576 1102 20-21 10.915601634772965 1102 22-23 11.227608632153462 1102 24-25 10.753648831937038 1102 26-27 10.97698301875673 1102 28-29 10.754756470551019 1102 30-31 10.702361008238473 1102 32-33 10.765777008679667 1102 34-35 10.61548530691 1102 36-37 10.579708009884591 1102 38-39 10.683206966892687 1102 40-41 10.18902590619091 1102 42-43 9.767762706090068 1102 44-45 9.723977867462718 1102 46-47 9.711499041118206 1102 48-49 9.417573588664606 1102 50-51 11.039362159908494 1102 52-53 10.428012340882884 1102 54-55 10.113397270942158 1102 56-57 9.997412131671965 1102 58-59 9.960317346042157 1102 60-61 9.826099939455773 1102 62-63 9.925066210152387 1102 64-65 9.629433385900594 1102 66-67 9.438650841321788 1102 68-69 9.192221926589374 1102 70-71 8.887445588723894 1102 72-73 8.797411863309941 1102 74-75 8.390792451275148 1102 76-77 8.125036530344582 1102 78-79 7.7192844489341965 1102 80-81 7.4206072122748825 1102 82-83 7.438146579804934 1102 84-85 7.080907723260228 1102 86-87 6.818382453077161 1102 88-89 6.5984533888230015 1102 90-91 6.22918175848835 1102 92-93 5.893666012421608 1102 94-95 5.534479036208182 1102 96-97 5.272855883370276 1102 98-99 4.97997291754228 1102 100-101 4.326400075619133 1103 1 7.862277237093231 1103 2 9.074234893812562 1103 3 8.556161460329385 1103 4 10.681807289873493 1103 5 11.227991316366875 1103 6 10.574039985885587 1103 7 10.252413567130755 1103 8 9.735296611451076 1103 9 9.682113975372555 1103 10-11 9.043392542486568 1103 12-13 8.951126161790047 1103 14-15 9.672551905735688 1103 16-17 10.309226512141311 1103 18-19 11.1267016047426 1103 20-21 11.582850448484155 1103 22-23 11.547056425407973 1103 24-25 11.275211183023586 1103 26-27 11.230800433296917 1103 28-29 10.954418783705908 1103 30-31 10.908966875071943 1103 32-33 11.028131370661793 1103 34-35 11.02688863031489 1103 36-37 11.053959863411336 1103 38-39 10.810590031200054 1103 40-41 10.472996452628664 1103 42-43 9.975617834629784 1103 44-45 9.947677440282519 1103 46-47 9.918660516923826 1103 48-49 9.986157829170335 1103 50-51 11.471211544813384 1103 52-53 11.100020098382313 1103 54-55 10.484998927255983 1103 56-57 10.164277613757971 1103 58-59 10.097703960499082 1103 60-61 9.989753487122343 1103 62-63 9.804967080678644 1103 64-65 9.63245402431469 1103 66-67 9.3674612031095 1103 68-69 9.289562748343338 1103 70-71 9.080507740642824 1103 72-73 8.809346494067976 1103 74-75 8.625654972790004 1103 76-77 8.195791116746877 1103 78-79 7.881099209314954 1103 80-81 7.481700637207711 1103 82-83 7.417772789206699 1103 84-85 7.246442216238645 1103 86-87 6.8734141561240545 1103 88-89 6.496367411808107 1103 90-91 6.24052121089891 1103 92-93 5.890849560436852 1103 94-95 5.603606734876561 1103 96-97 5.544291601889906 1103 98-99 5.0755576831742975 1103 100-101 4.380026722450043 1104 1 7.8222547225160355 1104 2 9.312840197641489 1104 3 8.66368697016421 1104 4 10.381045582520812 1104 5 11.21749209573353 1104 6 10.535375004365822 1104 7 10.096656655085251 1104 8 9.588612114540627 1104 9 9.237563979487543 1104 10-11 8.968950102922118 1104 12-13 8.702567028559908 1104 14-15 9.170017051358755 1104 16-17 10.143621747189936 1104 18-19 10.845925513536223 1104 20-21 10.93884379667556 1104 22-23 11.265557626199204 1104 24-25 11.08967084351005 1104 26-27 11.04561720872476 1104 28-29 10.90423658684728 1104 30-31 10.53602721322898 1104 32-33 10.489667072437111 1104 34-35 10.538964160848735 1104 36-37 10.634596499889003 1104 38-39 10.37812817963663 1104 40-41 10.122280010163458 1104 42-43 9.851572708700758 1104 44-45 9.834289724292084 1104 46-47 9.792824956877855 1104 48-49 9.582738706916263 1104 50-51 10.945558615871194 1104 52-53 10.3940892608144 1104 54-55 10.055625489246133 1104 56-57 9.697340626038013 1104 58-59 9.947730676915079 1104 60-61 9.540262091586627 1104 62-63 9.467060043053575 1104 64-65 9.367868660195992 1104 66-67 9.185518171110594 1104 68-69 8.89172615768887 1104 70-71 8.505103758242571 1104 72-73 8.342275200526108 1104 74-75 8.135801420933484 1104 76-77 7.812336311948345 1104 78-79 7.515213136218673 1104 80-81 7.345308267349944 1104 82-83 6.937800899342852 1104 84-85 6.594422707465036 1104 86-87 6.265060271966602 1104 88-89 6.043445582966042 1104 90-91 5.752304866655821 1104 92-93 5.50282308471674 1104 94-95 5.092734476979606 1104 96-97 4.602424930867004 1104 98-99 4.102175049919968 1104 100-101 3.710389681090464 1105 1 8.143621031474172 1105 2 9.120417203603612 1105 3 8.577469680072156 1105 4 10.68008092127187 1105 5 10.482576448064137 1105 6 10.549456071609892 1105 7 10.122232309062298 1105 8 9.719837180657443 1105 9 9.606131170793834 1105 10-11 9.092832619720927 1105 12-13 8.578758684001908 1105 14-15 9.434634460287207 1105 16-17 10.452156007227362 1105 18-19 10.762868282516628 1105 20-21 11.312632894642615 1105 22-23 11.39753371138029 1105 24-25 11.384785656711582 1105 26-27 11.624997687596025 1105 28-29 11.316782489715411 1105 30-31 11.071211036765785 1105 32-33 10.979272556519525 1105 34-35 10.84804074258182 1105 36-37 10.782630171674224 1105 38-39 10.623436684901147 1105 40-41 10.374727844866698 1105 42-43 10.136670148713204 1105 44-45 9.769882439894605 1105 46-47 9.749190573360739 1105 48-49 9.557342453277752 1105 50-51 11.02462029481313 1105 52-53 10.228974566208048 1105 54-55 10.12721280736082 1105 56-57 10.033575052128793 1105 58-59 9.991441845787183 1105 60-61 9.815944782740452 1105 62-63 9.826209972891458 1105 64-65 9.628712782508227 1105 66-67 9.381295700218175 1105 68-69 9.25626236911956 1105 70-71 9.104333703646576 1105 72-73 8.943550611302632 1105 74-75 8.556379685009576 1105 76-77 8.296682954006174 1105 78-79 8.048035558828783 1105 80-81 7.608031419762648 1105 82-83 7.455706460190525 1105 84-85 7.091583458409723 1105 86-87 6.889835117003907 1105 88-89 6.482797263309008 1105 90-91 6.130839731729681 1105 92-93 6.08054257776455 1105 94-95 5.672990901873579 1105 96-97 5.262905116727763 1105 98-99 4.7966230566867285 1105 100-101 4.173353662125689 1106 1 7.709140132509631 1106 2 8.973120666891969 1106 3 8.469896531342943 1106 4 10.248242691745666 1106 5 10.861801032312648 1106 6 10.063953639855892 1106 7 9.391660258544572 1106 8 8.925129971683486 1106 9 8.871797843997474 1106 10-11 8.291755195811294 1106 12-13 8.23538183077003 1106 14-15 8.65364293220437 1106 16-17 9.809515004717166 1106 18-19 10.97209924031393 1106 20-21 10.902224742383442 1106 22-23 11.155629295001255 1106 24-25 10.826276184637528 1106 26-27 10.970299303535779 1106 28-29 10.863530509012556 1106 30-31 10.680420030333401 1106 32-33 10.889619326601107 1106 34-35 10.682974310592488 1106 36-37 10.748233991881317 1106 38-39 10.46117750699089 1106 40-41 10.39839297861317 1106 42-43 10.226237286695635 1106 44-45 9.65490871852655 1106 46-47 9.43923891427696 1106 48-49 9.603887009286542 1106 50-51 11.273047204916674 1106 52-53 10.478412811422984 1106 54-55 9.99796253280795 1106 56-57 9.845901544519755 1106 58-59 9.41065848761335 1106 60-61 9.581799867851842 1106 62-63 9.530664102951079 1106 64-65 9.578422491874402 1106 66-67 9.204028040585293 1106 68-69 8.88723624737497 1106 70-71 8.595039696743536 1106 72-73 8.377405725830402 1106 74-75 7.787882518787425 1106 76-77 7.70887859253771 1106 78-79 7.426408784040582 1106 80-81 7.307607774877171 1106 82-83 7.142419032750924 1106 84-85 6.885404449937809 1106 86-87 6.659994308641808 1106 88-89 6.332119509935934 1106 90-91 5.682076347277219 1106 92-93 5.338613843845504 1106 94-95 5.169307186781015 1106 96-97 4.660375062993845 1106 98-99 4.18428347400709 1106 100-101 3.887814508925188 1107 1 7.372156134364918 1107 2 8.177897839124498 1107 3 8.15622422150992 1107 4 9.870569724831629 1107 5 10.585625243815429 1107 6 9.809135303430413 1107 7 9.913035876973762 1107 8 8.155093252450335 1107 9 5.43937572742357 1107 10-11 7.778657638606592 1107 12-13 7.892269489242512 1107 14-15 8.281701064548216 1107 16-17 9.158968081402385 1107 18-19 10.61068323072828 1107 20-21 10.854391467027842 1107 22-23 9.950399316646276 1107 24-25 10.031512154025286 1107 26-27 10.392322340023103 1107 28-29 10.603731305240139 1107 30-31 10.155782004668591 1107 32-33 10.551894083094613 1107 34-35 10.522342836411905 1107 36-37 10.783315547230728 1107 38-39 10.47519729672496 1107 40-41 10.109986303443101 1107 42-43 9.73169173552062 1107 44-45 9.276018025885854 1107 46-47 9.532853826150802 1107 48-49 9.82360717007813 1107 50-51 10.942248078447905 1107 52-53 10.106918338633868 1107 54-55 9.814151076407207 1107 56-57 9.49722817176156 1107 58-59 9.548056253537904 1107 60-61 9.36964357920002 1107 62-63 9.300887703755034 1107 64-65 9.329722256098275 1107 66-67 9.303407968692895 1107 68-69 8.771032629564209 1107 70-71 8.643536913812179 1107 72-73 8.22721478581803 1107 74-75 8.253207290741658 1107 76-77 8.051956823830226 1107 78-79 7.576931260076449 1107 80-81 7.3668388934609705 1107 82-83 7.150608484668053 1107 84-85 7.039033083209297 1107 86-87 6.532786033286213 1107 88-89 6.29600942954014 1107 90-91 5.959177750338442 1107 92-93 5.549773785094731 1107 94-95 5.286400569588682 1107 96-97 4.838804929258531 1107 98-99 4.52234083331445 1107 100-101 4.122212216872003 1108 1 6.691508959670124 1108 2 7.79428964837345 1108 3 7.657018444923189 1108 4 9.04753667322715 1108 5 10.73135350144845 1108 6 9.290357960843547 1108 7 8.544121678297657 1108 8 5.84668089760941 1108 9 6.979803245232041 1108 10-11 6.294548405687834 1108 12-13 4.952602123979908 1108 14-15 7.311945401340175 1108 16-17 8.401991856569015 1108 18-19 7.704726555128747 1108 20-21 7.99550985040813 1108 22-23 8.734772813519776 1108 24-25 8.608949795748643 1108 26-27 7.073648068967881 1108 28-29 8.963125416419963 1108 30-31 9.307306604407476 1108 32-33 9.850493169193701 1108 34-35 9.442466980345575 1108 36-37 9.06705343632576 1108 38-39 8.551424420571134 1108 40-41 8.812052315032922 1108 42-43 8.336173629288226 1108 44-45 7.737022916057413 1108 46-47 8.464366229091773 1108 48-49 8.998331453730984 1108 50-51 10.416909087632726 1108 52-53 9.835924385497059 1108 54-55 9.371257285894373 1108 56-57 9.278496066124692 1108 58-59 9.410438580205941 1108 60-61 9.405297167234558 1108 62-63 9.179294504185641 1108 64-65 9.1635363036028 1108 66-67 8.585970556017386 1108 68-69 8.615739333794723 1108 70-71 8.628650807854646 1108 72-73 8.258871775213118 1108 74-75 8.202483213231867 1108 76-77 7.903292172784626 1108 78-79 7.522147595768978 1108 80-81 7.47497467302532 1108 82-83 7.3996393259608 1108 84-85 7.120774820308178 1108 86-87 6.934089601851687 1108 88-89 6.595452843269266 1108 90-91 6.390508060240181 1108 92-93 6.159979584586242 1108 94-95 5.955047927521756 1108 96-97 5.661252763611129 1108 98-99 5.025379153019438 1108 100-101 4.486994678678273 1109 1 6.3718788943997815 1109 2 7.927858795919278 1109 3 7.855024062794307 1109 4 8.981765532340944 1109 5 9.833093223168738 1109 6 8.827343099812932 1109 7 7.337805740287436 1109 8 7.870578537194376 1109 9 7.200121812239267 1109 10-11 5.668381077983735 1109 12-13 6.25099115303146 1109 14-15 7.450402674456598 1109 16-17 6.315198304809218 1109 18-19 5.517377987629061 1109 20-21 7.530856080600682 1109 22-23 8.782850643574434 1109 24-25 8.322907724220222 1109 26-27 8.61278281074658 1109 28-29 9.1662465453734 1109 30-31 6.754201385810543 1109 32-33 5.644587443738267 1109 34-35 7.0965760193221605 1109 36-37 5.597842959356001 1109 38-39 6.157200827027708 1109 40-41 6.954434853037839 1109 42-43 5.7700231968674665 1109 44-45 6.425240492076718 1109 46-47 5.849720177701325 1109 48-49 6.587551476914644 1109 50-51 8.405991647230268 1109 52-53 8.949737700192546 1109 54-55 8.956071040018752 1109 56-57 8.006597070371246 1109 58-59 8.029337242599542 1109 60-61 7.954201246725731 1109 62-63 8.630485271254237 1109 64-65 8.514157044267776 1109 66-67 7.970257184878015 1109 68-69 7.697581828841676 1109 70-71 7.243655836663757 1109 72-73 6.667928508892688 1109 74-75 5.988726863294941 1109 76-77 5.765323054028967 1109 78-79 6.374697609217016 1109 80-81 6.444163131198129 1109 82-83 6.084688373422912 1109 84-85 5.851581958375977 1109 86-87 5.768778109270839 1109 88-89 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-13.50401375262503 2313 70-71 -13.144960303256466 2313 72-73 -12.7613442741696 2313 74-75 -12.304461231212576 2313 76-77 -11.89362140746229 2313 78-79 -11.506247465959419 2313 80-81 -11.073173475122827 2313 82-83 -10.622737217249076 2313 84-85 -10.237095550062193 2313 86-87 -9.76328694135819 2313 88-89 -9.337880490064068 2313 90-91 -8.872454902722781 2313 92-93 -8.355761156154497 2313 94-95 -7.810692813218985 2313 96-97 -7.206031187006156 2313 98-99 -6.586966525992908 2313 100-101 -5.728591741074813 2314 1 -18.34398486749037 2314 2 -17.13719183310803 2314 3 -17.748228468657057 2314 4 -19.233944808254332 2314 5 -18.554448967687353 2314 6 -19.49729636014411 2314 7 -19.63396474145543 2314 8 -20.054245028316515 2314 9 -21.229764656002526 2314 10-11 -21.556994804188708 2314 12-13 -21.44477441922997 2314 14-15 -21.38635706779563 2314 16-17 -20.274859995282835 2314 18-19 -19.272588259686067 2314 20-21 -18.94168150761656 2314 22-23 -18.571245704998745 2314 24-25 -18.40216131536247 2314 26-27 -18.10845069646422 2314 28-29 -17.906781990987444 2314 30-31 -17.8542674696666 2314 32-33 -17.704599423398893 2314 34-35 -17.36061943940751 2314 36-37 -16.914891008118683 2314 38-39 -16.778822493009113 2314 40-41 -16.60770077138683 2314 42-43 -16.431418963304367 2314 44-45 -16.031341281473452 2314 46-47 -15.700448585723041 2314 48-49 -15.408612990713461 2314 50-51 -13.324140295083323 2314 52-53 -15.063149688577017 2314 54-55 -15.765162467192049 2314 56-57 -15.701442205480244 2314 58-59 -15.43215401238665 2314 60-61 -15.127418882148156 2314 62-63 -14.692773397048924 2314 64-65 -14.327358758125595 2314 66-67 -13.94612820941471 2314 68-69 -13.50401375262503 2314 70-71 -13.144960303256466 2314 72-73 -12.7613442741696 2314 74-75 -12.304461231212576 2314 76-77 -11.89362140746229 2314 78-79 -11.506247465959419 2314 80-81 -11.073173475122827 2314 82-83 -10.622737217249076 2314 84-85 -10.237095550062193 2314 86-87 -9.76328694135819 2314 88-89 -9.337880490064068 2314 90-91 -8.872454902722781 2314 92-93 -8.355761156154497 2314 94-95 -7.810692813218985 2314 96-97 -7.206031187006156 2314 98-99 -6.586966525992908 2314 100-101 -5.728591741074813 2315 1 -18.34398486749037 2315 2 -17.13719183310803 2315 3 -17.748228468657057 2315 4 -19.233944808254332 2315 5 -18.554448967687353 2315 6 -19.49729636014411 2315 7 -19.63396474145543 2315 8 -20.054245028316515 2315 9 -21.229764656002526 2315 10-11 -21.556994804188708 2315 12-13 -21.44477441922997 2315 14-15 -21.38635706779563 2315 16-17 -20.274859995282835 2315 18-19 -19.272588259686067 2315 20-21 -18.94168150761656 2315 22-23 -18.571245704998745 2315 24-25 -18.40216131536247 2315 26-27 -18.10845069646422 2315 28-29 -17.906781990987444 2315 30-31 -17.8542674696666 2315 32-33 -17.704599423398893 2315 34-35 -17.36061943940751 2315 36-37 -16.914891008118683 2315 38-39 -16.778822493009113 2315 40-41 -16.60770077138683 2315 42-43 -16.431418963304367 2315 44-45 -16.031341281473452 2315 46-47 -15.700448585723041 2315 48-49 -15.408612990713461 2315 50-51 -13.324140295083323 2315 52-53 -15.063149688577017 2315 54-55 -15.765162467192049 2315 56-57 -15.701442205480244 2315 58-59 -15.43215401238665 2315 60-61 -15.127418882148156 2315 62-63 -14.692773397048924 2315 64-65 -14.327358758125595 2315 66-67 -13.94612820941471 2315 68-69 -13.50401375262503 2315 70-71 -13.144960303256466 2315 72-73 -12.7613442741696 2315 74-75 -12.304461231212576 2315 76-77 -11.89362140746229 2315 78-79 -11.506247465959419 2315 80-81 -11.073173475122827 2315 82-83 -10.622737217249076 2315 84-85 -10.237095550062193 2315 86-87 -9.76328694135819 2315 88-89 -9.337880490064068 2315 90-91 -8.872454902722781 2315 92-93 -8.355761156154497 2315 94-95 -7.810692813218985 2315 96-97 -7.206031187006156 2315 98-99 -6.586966525992908 2315 100-101 -5.728591741074813 2316 1 -18.34398486749037 2316 2 -17.13719183310803 2316 3 -17.748228468657057 2316 4 -19.233944808254332 2316 5 -18.554448967687353 2316 6 -19.49729636014411 2316 7 -19.63396474145543 2316 8 -20.054245028316515 2316 9 -21.229764656002526 2316 10-11 -21.556994804188708 2316 12-13 -21.44477441922997 2316 14-15 -21.38635706779563 2316 16-17 -20.274859995282835 2316 18-19 -19.272588259686067 2316 20-21 -18.94168150761656 2316 22-23 -18.571245704998745 2316 24-25 -18.40216131536247 2316 26-27 -18.10845069646422 2316 28-29 -17.906781990987444 2316 30-31 -17.8542674696666 2316 32-33 -17.704599423398893 2316 34-35 -17.36061943940751 2316 36-37 -16.914891008118683 2316 38-39 -16.778822493009113 2316 40-41 -16.60770077138683 2316 42-43 -16.431418963304367 2316 44-45 -16.031341281473452 2316 46-47 -15.700448585723041 2316 48-49 -15.408612990713461 2316 50-51 -13.324140295083323 2316 52-53 -15.063149688577017 2316 54-55 -15.765162467192049 2316 56-57 -15.701442205480244 2316 58-59 -15.43215401238665 2316 60-61 -15.127418882148156 2316 62-63 -14.692773397048924 2316 64-65 -14.327358758125595 2316 66-67 -13.94612820941471 2316 68-69 -13.50401375262503 2316 70-71 -13.144960303256466 2316 72-73 -12.7613442741696 2316 74-75 -12.304461231212576 2316 76-77 -11.89362140746229 2316 78-79 -11.506247465959419 2316 80-81 -11.073173475122827 2316 82-83 -10.622737217249076 2316 84-85 -10.237095550062193 2316 86-87 -9.76328694135819 2316 88-89 -9.337880490064068 2316 90-91 -8.872454902722781 2316 92-93 -8.355761156154497 2316 94-95 -7.810692813218985 2316 96-97 -7.206031187006156 2316 98-99 -6.586966525992908 2316 100-101 -5.728591741074813 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 828527.0 3 34502.0 4 117409.0 5 64329.0 6 40920.0 7 40572.0 8 39156.0 9 39191.0 10 41725.0 11 43686.0 12 44572.0 13 45080.0 14 45287.0 15 46970.0 16 48615.0 17 48758.0 18 48218.0 19 49394.0 20 51513.0 21 53985.0 22 57035.0 23 61255.0 24 64345.0 25 68725.0 26 72470.0 27 76652.0 28 81472.0 29 87478.0 30 93725.0 31 100211.0 32 106480.0 33 111303.0 34 111175.0 35 98949.0 36 67423.0 37 25469.0 38 2625.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24101204980027 20.70717157127048 14.250338094451958 22.801478284477295 2 14.897749603331963 20.718321298086558 41.685266170876176 22.698662927705303 3 18.386768393533586 26.64802859734301 30.442426505895536 24.522776503227874 4 12.227818173437447 17.579383019606727 39.83172415462828 30.36107465232754 5 12.497086363596466 38.24358683026593 35.91828554319448 13.34104126294313 6 28.986181847535725 39.26132966101063 18.062966763519935 13.689521727933709 7 25.854853676655637 33.44136385689293 23.36771572304635 17.336066743405095 8 24.476812583169778 36.85415831463915 20.700663470703926 17.968365631487142 9 25.258640332243125 17.150085175772837 21.903313324553704 35.68796116743033 10-11 23.775145227064773 27.572900620984274 29.00898373934062 19.642970412610328 12-13 24.484653340668128 25.020471338863427 29.435950292462955 21.05892502800549 14-15 21.878509253698883 26.711677774321185 26.852344298999554 24.557468672980377 16-17 21.012836439630238 29.886234978668526 27.763543043404226 21.337385538297006 18-19 20.870641902606387 28.529094827586203 30.010370415753663 20.589892854053744 20-21 21.47111634126871 27.805278882383146 30.151892064142928 20.57171271220522 22-23 21.36175498979078 27.619305846830343 29.863789455821838 21.155149707557037 24-25 21.15087032756186 28.009402916177734 29.971829191949027 20.867897564311374 26-27 20.979011309473602 28.12372528169978 29.878820813266245 21.018442595560373 28-29 20.984461164837352 28.076060194297824 30.04911661425073 20.89036202661409 30-31 21.231384434729485 27.915884111128364 29.857309474882094 20.99542197926006 32-33 21.024949671023073 28.255199543272635 29.708000284336926 21.011850501367366 34-35 21.202892951016832 28.131434448755694 29.78492247914365 20.88075012108382 36-37 21.26141447003586 27.951726779870295 29.791219615753008 20.995639134340834 38-39 21.1609598656646 28.125792232644482 29.77608949877741 20.937158402913507 40-41 21.218837193278087 28.100188483575067 29.844817225008917 20.836157098137928 42-43 21.19688682610832 28.128920893022098 29.478871881514596 21.195320399354987 44-45 21.169404188680776 28.45581123683788 29.3718453129326 21.002939261548747 46-47 21.4229996722401 28.322878661923035 29.087772741036787 21.166348924800083 48-49 21.339943182229767 28.496323292856957 28.86663590138235 21.297097623530924 50-51 21.523076215868343 28.691413073457756 28.51351475590696 21.27199595476694 52-53 21.07665606406908 28.89641246703635 28.623220320910157 21.403711147984417 54-55 20.969240482720977 28.875845471795934 28.761034123438456 21.393879922044633 56-57 21.053167926993833 28.903681540486115 28.765745102177952 21.277405430342096 58-59 21.036835174761475 28.795028743455532 28.811921913013038 21.356214168769956 60-61 21.008095908375477 28.973318204681302 28.80641347403153 21.21217241291169 62-63 20.949701169062163 28.845966382140908 28.86490066311051 21.33943178568642 64-65 20.734855115426694 28.732764214217323 28.87997943014463 21.652401240211354 66-67 20.725289211196028 28.771374380764676 28.6496023686118 21.853734039427504 68-69 20.81318395712883 28.771352392616123 28.74916780778895 21.666295842466095 70-71 20.972777134123536 28.584825070034924 28.797276886916507 21.64512090892503 72-73 20.898163271911617 28.72057333045345 28.6624820950585 21.718781302576428 74-75 20.769463113358846 29.095493735121003 28.435686681916756 21.699356469603394 76-77 20.87993952768091 28.91830034094529 28.511563018107 21.690197113266798 78-79 20.743154727134925 28.929190158456024 28.436418651853657 21.891236462555394 80-81 20.775743620787267 28.96967362628031 28.306862977904977 21.947719775027437 82-83 21.000308153012675 28.683355517792958 28.508202709274467 21.808133619919897 84-85 20.954030945985178 28.815157759125658 28.265435345280626 21.96537594960854 86-87 20.786591170075262 28.83579700347216 28.460912332909455 21.916699493543117 88-89 21.023893511253778 28.711968084339823 28.237280696156013 22.026857708250382 90-91 20.924875389726957 28.662054767107985 28.421649162810304 21.991420680354754 92-93 20.911674961519264 28.716737919554898 28.180555584562594 22.191031534363244 94-95 20.697702004235186 28.522295582142082 28.395900142032417 22.38410227159032 96-97 20.7692417244817 28.478525360623387 28.299119041321642 22.453113873573276 98-99 20.594582128933425 28.573912425368263 28.08663278662968 22.744872659068633 100-101 20.8848075485318 28.577875170795846 28.040093841037404 22.497223439634947 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 187278.0 1 169858.0 2 112721.5 3 57085.5 4 35354.0 5 27744.5 6 25735.0 7 26040.0 8 26849.5 9 26624.0 10 24524.5 11 20875.0 12 16747.0 13 12857.0 14 9767.0 15 7924.0 16 7354.0 17 7839.0 18 8907.5 19 10307.5 20 12116.0 21 13913.5 22 15689.0 23 17800.0 24 19975.0 25 22432.0 26 25650.0 27 29444.0 28 33484.0 29 38414.5 30 44385.5 31 50583.5 32 57174.5 33 63404.5 34 69051.5 35 74213.0 36 80106.0 37 85990.5 38 88445.0 39 88563.0 40 87613.0 41 86246.5 42 85324.0 43 85546.5 44 86351.5 45 86628.0 46 85653.5 47 83687.5 48 81621.0 49 78827.5 50 74846.0 51 70236.0 52 65564.5 53 59822.0 54 53560.5 55 47150.0 56 40167.5 57 33195.5 58 26933.0 59 21684.0 60 17009.0 61 12942.0 62 9545.0 63 6796.0 64 4583.5 65 2939.5 66 1897.0 67 1206.5 68 757.5 69 462.5 70 292.5 71 186.0 72 126.5 73 77.0 74 38.5 75 26.5 76 21.5 77 15.5 78 13.5 79 9.0 80 6.5 81 5.0 82 2.0 83 2.0 84 2.0 85 1.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.580709483125236 2 29.686393199819694 3 23.415760850911656 4 29.79295655451255 5 32.966354418603736 6 25.490869710608578 7 24.404806072672937 8 20.337910436442314 9 12.058460787137083 10-11 15.742986840079459 12-13 15.829937307405531 14-15 20.037898636021747 16-17 23.403470108161805 18-19 27.439638887517802 20-21 29.52850945651708 22-23 29.111572159244876 24-25 27.628641745442373 26-27 29.079897346147522 28-29 25.99072048922386 30-31 27.901473352787136 32-33 26.883711595106323 34-35 24.916555336290738 36-37 27.939195472822586 38-39 28.051495597070225 40-41 25.04483996374231 42-43 25.918708504442158 44-45 24.614876440443997 46-47 27.943641060625723 48-49 23.28742719124167 50-51 28.890208037165117 52-53 22.44119647765618 54-55 23.583352973460823 56-57 25.74238864701477 58-59 25.019065360927794 60-61 24.549124235546856 62-63 24.38367318653523 64-65 22.895479981289306 66-67 23.4014270990611 68-69 24.411392734013617 70-71 22.876945802727693 72-73 22.740799676256607 74-75 19.969890949077428 76-77 22.26930586395913 78-79 21.37608594105739 80-81 22.431014120298258 82-83 23.305863305366444 84-85 20.227408160203293 86-87 21.790261090025442 88-89 20.61190247295628 90-91 21.75559531160465 92-93 21.74278155852502 94-95 20.772352235689375 96-97 19.66724775293515 98-99 19.06444565971706 100-101 20.75371999269093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3059213.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.12074787866696 #Duplication Level Percentage of deduplicated Percentage of total 1 86.43976633133656 60.612210616105344 2 8.100977515471165 11.360932038662067 3 2.464076696602455 5.183487023884779 4 1.0146686724870222 2.845973046553767 5 0.5411825617244834 1.8974062983506808 6 0.35047058744155646 1.4745155820526608 7 0.2213826186427792 1.0866460350598577 8 0.16151029922270596 0.9060178377282738 9 0.11664329117616624 0.7361203331071703 >10 0.5547137233742317 6.731632028274513 >50 0.025197666613275008 1.1890904601182777 >100 0.009120872104850027 1.0665496823247764 >500 9.638793426508631E-5 0.04136381950681494 >1k 1.4458190136927432E-4 0.18556269025349625 >5k 0.0 0.0 >10k+ 4.8193967123091434E-5 4.682492508017563 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 138560 4.529269455902547 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.2688145611305914E-5 0.0 0.0 0.0 6 0.0 3.2688145611305914E-5 0.0 0.0 0.0 7 0.0 3.2688145611305914E-5 0.0 3.2688145611305914E-5 0.0 8 0.0 3.2688145611305914E-5 0.0 3.2688145611305914E-5 0.0 9 0.0 3.2688145611305914E-5 0.0 6.537629122261183E-5 0.0 10-11 0.0 3.2688145611305914E-5 0.0 8.172036402826478E-5 0.0 12-13 0.0 4.903221841695887E-5 0.0 9.806443683391774E-5 0.0 14-15 0.0 6.537629122261183E-5 0.0 9.806443683391774E-5 0.0 16-17 0.0 8.172036402826478E-5 0.0 9.806443683391774E-5 0.0 18-19 0.0 9.806443683391774E-5 0.0 9.806443683391774E-5 0.0 20-21 0.0 9.806443683391774E-5 0.0 1.144085096395707E-4 0.0 22-23 0.0 9.806443683391774E-5 0.0 1.3075258244522365E-4 0.0 24-25 0.0 9.806443683391774E-5 0.0 1.3075258244522365E-4 0.0 26-27 0.0 9.806443683391774E-5 0.0 1.7978480086218253E-4 0.0 28-29 0.0 9.806443683391774E-5 0.0 4.903221841695887E-4 0.0 30-31 0.0 9.806443683391774E-5 0.0 0.0013729021156748485 0.0 32-33 0.0 9.806443683391774E-5 0.0 0.003857201182134098 0.0 34-35 0.0 9.806443683391774E-5 0.0 0.0072404242529042605 0.0 36-37 0.0 1.144085096395707E-4 0.0 0.013516548210274995 0.0 38-39 0.0 1.3075258244522365E-4 0.0 0.02337202411208373 0.0 40-41 0.0 1.3075258244522365E-4 0.0 0.03350534925158856 0.0 42-43 0.0 1.3075258244522365E-4 0.0 0.043622330318287744 0.0 44-45 0.0 1.3075258244522365E-4 0.0 0.05651780376194793 0.0 46-47 0.0 1.3075258244522365E-4 0.0 0.07318875802371394 0.0 48-49 0.0 1.3075258244522365E-4 0.0 0.09196809767740918 0.0 50-51 0.0 1.3075258244522365E-4 0.0 0.11498055218776855 0.0 52-53 0.0 1.3075258244522365E-4 0.0 0.1419973045355129 0.0 54-55 3.2688145611305914E-5 1.3075258244522365E-4 0.0 0.171661796677773 0.0 56-57 3.2688145611305914E-5 1.6344072805652958E-4 0.0 0.20814176717999042 0.0 58-59 3.2688145611305914E-5 1.6344072805652958E-4 0.0 0.25235248411928163 0.0 60-61 3.2688145611305914E-5 1.6344072805652958E-4 0.0 0.2975765335725234 0.0 62-63 3.2688145611305914E-5 1.6344072805652958E-4 0.0 0.34481090398086045 0.0 64-65 3.2688145611305914E-5 1.6344072805652958E-4 0.0 0.39439882087321154 0.0 66-67 3.2688145611305914E-5 1.6344072805652958E-4 0.0 0.4539075899585939 0.0 68-69 6.537629122261183E-5 1.6344072805652958E-4 0.0 0.5216701158108311 0.0 70-71 6.537629122261183E-5 1.6344072805652958E-4 0.0 0.5967711303528064 0.0 72-73 8.172036402826478E-5 1.6344072805652958E-4 0.0 0.678736655473156 0.0 74-75 9.806443683391774E-5 1.7978480086218253E-4 0.0 0.7618462656899012 0.0 76-77 9.806443683391774E-5 1.9612887366783548E-4 0.0 0.8561025335601019 0.0 78-79 9.806443683391774E-5 1.9612887366783548E-4 0.0 0.9665067453622876 0.0 80-81 9.806443683391774E-5 1.9612887366783548E-4 0.0 1.0873057874688685 0.0 82-83 9.806443683391774E-5 1.9612887366783548E-4 0.0 1.2133349328732586 0.0 84-85 9.806443683391774E-5 1.9612887366783548E-4 0.0 1.3389391323847017 0.0 86-87 9.806443683391774E-5 1.9612887366783548E-4 0.0 1.4740228941234232 0.0 88-89 9.806443683391774E-5 1.9612887366783548E-4 0.0 1.621577183412858 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2320 0.0 57.495808 1 GTATCAA 4915 0.0 46.8677 1 ATCAACG 6035 0.0 37.598377 3 TCAACGC 6135 0.0 36.981865 4 CAACGCA 6335 0.0 36.048634 5 TATCAAC 6285 0.0 35.91052 2 AACGCAG 6825 0.0 32.492023 6 ACGCAGA 7850 0.0 27.715761 7 GTACATG 5565 0.0 27.564924 1 CGCAGAG 8090 0.0 26.828733 8 TACATGG 5710 0.0 26.05171 2 ACATGGG 5640 0.0 25.927025 3 GTGGTAT 1420 0.0 25.290678 1 GCAGAGT 8655 0.0 23.407099 9 TGGTATC 1365 0.0 22.547216 2 GAGTACT 5325 0.0 20.799292 12-13 AGAGTAC 7675 0.0 20.30536 10-11 CATGGGG 3325 0.0 20.167444 4 CAGAGTA 8295 0.0 19.394606 10-11 GTATAGG 945 0.0 18.458563 1 >>END_MODULE