FastQCFastQC Report
Sat 18 Jun 2016
SRR1930999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059213
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86260.2819679440431248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77300.2526793655753947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42220.13800935077093357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43400.061.3255161
GTATCAA88050.044.261631
ATCAACG109100.035.150773
TCAACGC112350.034.3031354
CAACGCA113050.034.036135
TATCAAC115500.033.2468872
AACGCAG117650.032.9059076
ACGCAGA135650.028.1547557
CGCAGAG136850.027.8038678
GCAGAGT151100.024.679359
GTACATG88750.023.562821
GTGGTAT21550.023.3776861
ACATGGG89200.022.002463
TACATGG93300.021.903242
GAGTACT104000.021.8057112-13
AGAGTAC141400.021.44011510-11
CAGAGTA145800.021.11848810-11
TGGTATC21750.020.758022
ACGTCCG1150.003750495620.627566
GTACTTT113250.019.81520714-15
Produced by FastQC (version 0.11.5)