##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930999_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3059213 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35696958662244 31.0 31.0 34.0 30.0 34.0 2 31.52647363880841 31.0 31.0 34.0 30.0 34.0 3 31.58766061728948 31.0 31.0 34.0 30.0 34.0 4 35.364485245061395 37.0 35.0 37.0 33.0 37.0 5 35.23795629791061 37.0 35.0 37.0 33.0 37.0 6 35.26660778442037 37.0 35.0 37.0 32.0 37.0 7 35.14771249991419 37.0 35.0 37.0 32.0 37.0 8 35.15457864489985 37.0 35.0 37.0 32.0 37.0 9 36.65120866052805 39.0 35.0 39.0 32.0 39.0 10-11 36.59929710680492 39.0 35.0 39.0 32.0 39.0 12-13 36.483218396365345 38.0 35.0 39.0 32.0 39.0 14-15 37.54435993832401 39.0 36.0 41.0 32.0 41.0 16-17 37.500312662112776 39.0 36.0 41.0 32.0 41.0 18-19 37.53690295510643 39.0 36.0 41.0 32.0 41.0 20-21 37.52896839808147 39.0 36.0 41.0 32.0 41.0 22-23 37.426154373690224 39.0 36.0 41.0 32.0 41.0 24-25 37.29971482861768 39.0 36.0 41.0 31.5 41.0 26-27 37.05011632076616 39.0 36.0 40.0 31.0 41.0 28-29 36.87305574995922 39.0 36.0 40.0 30.5 41.0 30-31 36.64163789837452 38.5 35.0 40.0 30.0 41.0 32-33 36.471630448746126 38.0 35.0 40.0 30.0 41.0 34-35 36.54531492249804 38.0 35.0 40.0 30.0 41.0 36-37 36.605040577429556 39.0 35.0 40.0 30.0 41.0 38-39 36.586509667682506 39.0 35.0 40.0 30.0 41.0 40-41 36.47199655597698 39.0 35.0 40.0 30.0 41.0 42-43 36.32688374428325 39.0 35.0 40.0 30.0 41.0 44-45 36.14265580722885 38.0 35.0 40.0 30.0 41.0 46-47 35.88530726693433 38.0 35.0 40.0 29.0 41.0 48-49 35.65356580270809 38.0 35.0 40.0 28.0 41.0 50-51 35.3854177528665 38.0 34.0 40.0 27.0 41.0 52-53 35.17594230934557 38.0 34.0 40.0 26.5 41.0 54-55 35.02147741919245 38.0 34.0 40.0 26.0 41.0 56-57 34.84527883478529 38.0 34.0 40.0 26.0 41.0 58-59 34.638476791253176 38.0 33.5 40.0 26.0 41.0 60-61 34.32587842036497 37.0 33.0 40.0 25.5 41.0 62-63 34.067421261612054 37.0 33.0 40.0 25.0 41.0 64-65 33.75409296443236 36.5 32.5 39.5 24.5 41.0 66-67 33.43014363498063 36.0 32.0 39.0 23.0 41.0 68-69 33.08015198680184 36.0 32.0 39.0 23.0 40.0 70-71 32.70790379747994 35.0 31.0 38.5 22.0 40.0 72-73 32.28798942734618 35.0 31.0 38.0 22.0 40.0 74-75 31.777774218401923 35.0 31.0 37.0 20.0 39.0 76-77 30.38842784075512 33.5 29.0 36.0 19.5 38.5 78-79 31.12429912529791 34.0 30.0 36.0 20.0 39.0 80-81 31.098846991039853 34.0 31.0 36.0 20.0 38.5 82-83 30.84215074269101 34.0 30.0 35.5 20.0 37.0 84-85 30.508525395256886 34.0 30.0 35.0 18.5 37.0 86-87 30.179028233732012 34.0 30.0 35.0 18.0 36.0 88-89 29.84184903110702 34.0 30.0 35.0 15.0 36.0 90-91 29.516463547977864 34.0 29.0 35.0 9.0 36.0 92-93 29.2064512997297 34.0 29.0 35.0 4.5 35.0 94-95 28.832148170133955 34.0 29.0 35.0 2.0 35.0 96-97 28.421222222839667 34.0 29.0 35.0 2.0 35.0 98-99 27.94470391568027 34.0 29.0 35.0 2.0 35.0 100-101 26.827582780277147 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2984976972700295 1101 2 0.3851437410197178 1101 3 0.2719621503795473 1101 4 0.19335836304805554 1101 5 0.2532253888808924 1101 6 0.24809060319174137 1101 7 0.257748430135301 1101 8 0.3756726101608834 1101 9 0.4346268828935749 1101 10-11 0.41345117692033995 1101 12-13 0.3030770192705319 1101 14-15 0.4418558001213242 1101 16-17 0.521663816682512 1101 18-19 0.4669808106876445 1101 20-21 0.5114924374788572 1101 22-23 0.35795408826147224 1101 24-25 0.437015455315354 1101 26-27 0.45309291868821333 1101 28-29 0.5231095412885267 1101 30-31 0.44858070057281196 1101 32-33 0.3968865525167402 1101 34-35 0.32840446853536776 1101 36-37 0.4099825560336825 1101 38-39 0.35464879110914893 1101 40-41 0.4393165208676777 1101 42-43 0.43230486211134433 1101 44-45 0.42996944053957975 1101 46-47 0.3202562821440651 1101 48-49 0.3262366492956872 1101 50-51 0.295532648847491 1101 52-53 0.11745247617470511 1101 54-55 0.27081529578314445 1101 56-57 0.2974652873048953 1101 58-59 0.2460038654564798 1101 60-61 0.2388169101243065 1101 62-63 0.18227075819584826 1101 64-65 0.20006429184568475 1101 66-67 0.24216620363662145 1101 68-69 0.20462672342939925 1101 70-71 0.15252548463578108 1101 72-73 0.2502513960956989 1101 74-75 0.11736790973625943 1101 76-77 0.1451515945440427 1101 78-79 0.11985050356332039 1101 80-81 0.14917258757627394 1101 82-83 0.16539823950991206 1101 84-85 0.1595426769717001 1101 86-87 0.07420386566988668 1101 88-89 0.0796153214209383 1101 90-91 0.128082375856458 1101 92-93 -8.271630202436597E-4 1101 94-95 0.013661538376990023 1101 96-97 0.21025908969791018 1101 98-99 0.30276238478251116 1101 100-101 0.6850975254194616 1102 1 0.2899750487831483 1102 2 0.4224000024225738 1102 3 0.3245732200615734 1102 4 0.1601763944907333 1102 5 0.2450340032435463 1102 6 0.2697959626839932 1102 7 0.40224334163658426 1102 8 0.29902642268620383 1102 9 0.39175498466136816 1102 10-11 0.4251007327071221 1102 12-13 0.414251408306761 1102 14-15 0.5452791407226414 1102 16-17 0.43668064732465695 1102 18-19 0.49907181734893413 1102 20-21 0.5317384498231519 1102 22-23 0.5292580344820337 1102 24-25 0.547931024778471 1102 26-27 0.4969610620985989 1102 28-29 0.5363716287248863 1102 30-31 0.4911891935233754 1102 32-33 0.6334552305989121 1102 34-35 0.6106565209128902 1102 36-37 0.6363564397685053 1102 38-39 0.6299825924759048 1102 40-41 0.5972600598773212 1102 42-43 0.5895416560896578 1102 44-45 0.7248589959332961 1102 46-47 0.7350301794176417 1102 48-49 0.7247792674513747 1102 50-51 0.583041560953653 1102 52-53 0.5974070885786062 1102 54-55 0.5903011723467912 1102 56-57 0.7042358129908735 1102 58-59 0.673220536909021 1102 60-61 0.5399544387986381 1102 62-63 0.3539220172458002 1102 64-65 0.40922408085941697 1102 66-67 0.5195402631569337 1102 68-69 0.6226327809320011 1102 70-71 0.5979396123302863 1102 72-73 0.6089085586994614 1102 74-75 0.48802390625985836 1102 76-77 0.36822726201029354 1102 78-79 0.5773374916153635 1102 80-81 0.6500671828820366 1102 82-83 0.5505880387104689 1102 84-85 0.6691334679955041 1102 86-87 0.6520445756249131 1102 88-89 0.6627927042697301 1102 90-91 0.5248468855295734 1102 92-93 0.5733830851648527 1102 94-95 0.7504518044463495 1102 96-97 0.7783895072485087 1102 98-99 0.9480422713189789 1102 100-101 0.9824309905009301 1103 1 0.39856979181465846 1103 2 0.4759141750211242 1103 3 0.4286148962815908 1103 4 0.29437944402693716 1103 5 0.3961672511337966 1103 6 0.37322502678637903 1103 7 0.40216642172944006 1103 8 0.3301910302309068 1103 9 0.5456755225935552 1103 10-11 0.47785854382860293 1103 12-13 0.45173702544637706 1103 14-15 0.4739525153405495 1103 16-17 0.7117164922127088 1103 18-19 0.6280740034380585 1103 20-21 0.6716702362351583 1103 22-23 0.6930138074372891 1103 24-25 0.6194886212004249 1103 26-27 0.6037657795711553 1103 28-29 0.5738868832737793 1103 30-31 0.6683285548556981 1103 32-33 0.7281627077844419 1103 34-35 0.6311889588196067 1103 36-37 0.7334975656096816 1103 38-39 0.7141618686891675 1103 40-41 0.8053775402431924 1103 42-43 0.7859418466988686 1103 44-45 0.7305383371269798 1103 46-47 0.6531927868334435 1103 48-49 0.5063430555032298 1103 50-51 0.4825263666305091 1103 52-53 0.5189261722173839 1103 54-55 0.5312999054928227 1103 56-57 0.5960585823399853 1103 58-59 0.6111726394745389 1103 60-61 0.6142034923562321 1103 62-63 0.5174499889375923 1103 64-65 0.4781430497522905 1103 66-67 0.5436101186890951 1103 68-69 0.38222489410569693 1103 70-71 0.5062087664965915 1103 72-73 0.4414118684743116 1103 74-75 0.47712410183506293 1103 76-77 0.507519598864544 1103 78-79 0.5965404724730838 1103 80-81 0.5991731474502764 1103 82-83 0.6335071703185307 1103 84-85 0.5294558448206317 1103 86-87 0.5471226525004411 1103 88-89 0.6237053991185313 1103 90-91 0.49176102368322105 1103 92-93 0.4485614115017853 1103 94-95 0.5538851477468718 1103 96-97 0.6805927659899602 1103 98-99 0.7070243610257627 1103 100-101 0.9432486448875288 1104 1 0.3261815386009346 1104 2 0.32769865377599317 1104 3 0.3161596700584788 1104 4 0.17598312377899816 1104 5 0.29231380101471416 1104 6 0.2892777879858741 1104 7 0.2660458686582743 1104 8 0.31077882446817995 1104 9 0.4722167516893023 1104 10-11 0.3751046644685232 1104 12-13 0.3791757979139092 1104 14-15 0.4377751381895436 1104 16-17 0.4471062106936401 1104 18-19 0.4638042321529099 1104 20-21 0.3844348517896563 1104 22-23 0.3717012414992311 1104 24-25 0.32618083526757147 1104 26-27 0.4251341770287027 1104 28-29 0.3931486747873052 1104 30-31 0.39161906610285513 1104 32-33 0.4585342065795146 1104 34-35 0.4829568949020313 1104 36-37 0.4601329018333047 1104 38-39 0.5514830889263109 1104 40-41 0.4090765231583404 1104 42-43 0.4764507778809417 1104 44-45 0.5289897568811597 1104 46-47 0.43119870414737704 1104 48-49 0.45901767478395783 1104 50-51 0.39183633454185696 1104 52-53 0.5208314745161147 1104 54-55 0.5689436871642926 1104 56-57 0.7076741645448621 1104 58-59 0.7031541373225352 1104 60-61 0.6373098836590927 1104 62-63 0.5058062221545256 1104 64-65 0.41216073965906475 1104 66-67 0.38333943507556256 1104 68-69 0.39245085046860595 1104 70-71 0.5374074195922063 1104 72-73 0.4112593326273739 1104 74-75 0.5190048754862211 1104 76-77 0.5189196714041664 1104 78-79 0.7790670472424495 1104 80-81 0.6838382348431473 1104 82-83 0.7196038192280163 1104 84-85 0.6921335906664581 1104 86-87 0.6606763121738339 1104 88-89 0.7802472609714997 1104 90-91 0.4747187625314986 1104 92-93 0.38395669245633 1104 94-95 0.649856221229836 1104 96-97 0.7496044529744772 1104 98-99 0.8388611890675826 1104 100-101 1.015825484567113 1105 1 0.36570639806062033 1105 2 0.366094748416554 1105 3 0.30426095516792984 1105 4 0.31415555594707456 1105 5 0.3251036602020321 1105 6 0.3415545441141745 1105 7 0.3155360400323275 1105 8 0.2758970668795442 1105 9 0.4452400664969929 1105 10-11 0.41312306360643447 1105 12-13 0.3774610924932844 1105 14-15 0.5126099039449628 1105 16-17 0.5726805179118486 1105 18-19 0.5870391991583261 1105 20-21 0.4264206617756514 1105 22-23 0.5857712733611322 1105 24-25 0.5455957911652334 1105 26-27 0.5227109759517887 1105 28-29 0.43062837038764457 1105 30-31 0.39313577787253706 1105 32-33 0.5840232860738723 1105 34-35 0.5350109332991337 1105 36-37 0.5341060434417741 1105 38-39 0.6026071099381625 1105 40-41 0.44432210664361804 1105 42-43 0.48871130858031364 1105 44-45 0.5675665584173473 1105 46-47 0.5491459843678896 1105 48-49 0.4906381653780514 1105 50-51 0.42411261628944175 1105 52-53 0.3273589131194825 1105 54-55 0.41642078573115526 1105 56-57 0.4871105439585861 1105 58-59 0.5398581199296544 1105 60-61 0.4833371561287052 1105 62-63 0.43366803947090915 1105 64-65 0.3963954198249482 1105 66-67 0.5366156671739688 1105 68-69 0.4216933569114403 1105 70-71 0.39480299502707794 1105 72-73 0.42727732050132516 1105 74-75 0.5954747974365198 1105 76-77 0.6196823087760812 1105 78-79 0.8074751159761959 1105 80-81 0.6660120222124384 1105 82-83 0.7904879081165141 1105 84-85 0.8019863477129725 1105 86-87 0.8374792152230768 1105 88-89 0.8616696373093866 1105 90-91 0.7815145981119365 1105 92-93 0.6645961094343775 1105 94-95 0.8458306886922422 1105 96-97 0.6866388035663569 1105 98-99 0.8331868618892031 1105 100-101 0.8818802670987296 1106 1 0.2593561862626892 1106 2 0.2753367863832956 1106 3 0.30975171842490923 1106 4 0.21205395177387487 1106 5 0.19781428147595648 1106 6 0.19163004615371193 1106 7 0.31494280972169264 1106 8 0.2972782639300675 1106 9 0.3811637407988471 1106 10-11 0.3070726537382171 1106 12-13 0.33231401059808263 1106 14-15 0.31558703761299256 1106 16-17 0.35808945460779995 1106 18-19 0.3434291720952558 1106 20-21 0.339269392949177 1106 22-23 0.2771666883281867 1106 24-25 0.28940913443477 1106 26-27 0.38500064884164686 1106 28-29 0.4205665959916587 1106 30-31 0.4769942262566005 1106 32-33 0.39372809271020515 1106 34-35 0.5108554775413694 1106 36-37 0.4599775526981347 1106 38-39 0.6065826845381039 1106 40-41 0.4648869011211829 1106 42-43 0.3725098946330192 1106 44-45 0.4642501068170972 1106 46-47 0.4510354474209137 1106 48-49 0.4623393636052242 1106 50-51 0.3859417757654384 1106 52-53 0.5024513295769708 1106 54-55 0.5665660045890206 1106 56-57 0.5742918174916909 1106 58-59 0.5544012345358666 1106 60-61 0.5206369776693407 1106 62-63 0.36456082657476685 1106 64-65 0.3483817117923138 1106 66-67 0.3252736919621739 1106 68-69 0.27786805098109824 1106 70-71 0.3340085566838056 1106 72-73 0.2869340803852296 1106 74-75 0.6213647409570733 1106 76-77 0.6009998683932736 1106 78-79 0.505674490387868 1106 80-81 0.499252432472872 1106 82-83 0.5167879159608795 1106 84-85 0.5291401169316714 1106 86-87 0.4213216316805557 1106 88-89 0.43755717849231957 1106 90-91 0.39344063552957564 1106 92-93 0.4345121256722173 1106 94-95 0.6008827066558418 1106 96-97 0.48979013122559323 1106 98-99 0.5916421587985212 1106 100-101 0.817931706540211 1107 1 0.15120490302212275 1107 2 0.21510294284588127 1107 3 0.19346248682317935 1107 4 0.057957438168429576 1107 5 0.11654263645431229 1107 6 0.1792015520287933 1107 7 0.28234965882496965 1107 8 0.2545073146412591 1107 9 0.18835220709445366 1107 10-11 0.20069307349703536 1107 12-13 0.2818633303259759 1107 14-15 0.29655835795930585 1107 16-17 0.2857294708415665 1107 18-19 0.36081427954732703 1107 20-21 0.3575359552559121 1107 22-23 0.3508840275365941 1107 24-25 0.2340508320228949 1107 26-27 0.24907667698017377 1107 28-29 0.29585145993723216 1107 30-31 0.35168462514342735 1107 32-33 0.3543982187151471 1107 34-35 0.31024806412145267 1107 36-37 0.3757304902367764 1107 38-39 0.35160635878052204 1107 40-41 0.32704521899378136 1107 42-43 0.3076972593516345 1107 44-45 0.31773795081833356 1107 46-47 0.43466939531825943 1107 48-49 0.3854661995668849 1107 50-51 0.37179839682294613 1107 52-53 0.34840689930486235 1107 54-55 0.41363407045172806 1107 56-57 0.611404429581988 1107 58-59 0.5458588914622737 1107 60-61 0.546057606148004 1107 62-63 0.21344243603673618 1107 64-65 0.22077909119862227 1107 66-67 0.3136878076084315 1107 68-69 0.30077080299098924 1107 70-71 0.41249997597879684 1107 72-73 0.25693282420093055 1107 74-75 0.27487509964600676 1107 76-77 0.3097817561483751 1107 78-79 0.3521491541169972 1107 80-81 0.32098461398183886 1107 82-83 0.5034302319781965 1107 84-85 0.4333889187251181 1107 86-87 0.382336725217975 1107 88-89 0.4840231455609647 1107 90-91 0.5495840531238834 1107 92-93 0.4722852974409264 1107 94-95 0.5477079424319733 1107 96-97 0.5563633571068571 1107 98-99 0.7023457186315447 1107 100-101 0.8125480871833766 1108 1 0.1258916800898504 1108 2 0.19400190984008603 1108 3 0.21734816904219656 1108 4 0.10873219251461563 1108 5 0.21711983703151105 1108 6 0.06913776220309842 1108 7 0.15163262453651072 1108 8 0.24511005405351938 1108 9 0.2284939877124259 1108 10-11 0.24925436670118017 1108 12-13 0.2548603068943862 1108 14-15 0.2708108030450873 1108 16-17 0.250359902138662 1108 18-19 0.27520965049031076 1108 20-21 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-1.6456859800790617 2316 78-79 -1.3797054900002372 2316 80-81 -1.184223775206732 2316 82-83 -0.947805911454477 2316 84-85 -0.8681980849675845 2316 86-87 -0.8325879729850101 2316 88-89 -0.7976615198974422 2316 90-91 -0.78275014172063 2316 92-93 -0.7771052102072211 2316 94-95 -1.3127585247644724 2316 96-97 -1.168279258741375 2316 98-99 -1.1432015912014784 2316 100-101 -1.5930744350782895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 17.0 4 31.0 5 63.0 6 135.0 7 359.0 8 972.0 9 1826.0 10 3191.0 11 5610.0 12 7805.0 13 10279.0 14 12822.0 15 16612.0 16 20181.0 17 23720.0 18 20430.0 19 15618.0 20 14559.0 21 15424.0 22 16856.0 23 19814.0 24 24163.0 25 31342.0 26 40507.0 27 52315.0 28 66892.0 29 83535.0 30 103355.0 31 124604.0 32 150537.0 33 183651.0 34 228050.0 35 286218.0 36 377117.0 37 518608.0 38 489782.0 39 92196.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.25889457723115 21.91646973946114 15.135679548413691 22.68895613489402 2 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20.17373422510953 20-21 20.966814013931035 28.004293914807498 30.72226419016917 20.306627881092293 22-23 20.92687821294719 27.705584899603803 30.57088860846014 20.79664827898887 24-25 20.755167834596605 28.16125196641265 30.505109608351894 20.578470590638855 26-27 20.632663264920854 28.257040311161834 30.49860327274338 20.611693151173927 28-29 20.601486038945065 28.136789719817866 30.647980121642654 20.61374411959441 30-31 20.917040046498947 27.97002870878465 30.469314421669345 20.643616823047054 32-33 20.781718999071945 28.274211744388623 30.28755699407708 20.656512262462353 34-35 20.925573448708533 28.126432354923903 30.42812250921448 20.51987168715309 36-37 20.956861927243832 27.974631739189988 30.481760290431055 20.586746043135133 38-39 20.723969372424982 28.179389148288248 30.524819528217144 20.571821951069623 40-41 20.79843785957865 28.132616529634515 30.5064240360677 20.562521574719135 42-43 20.810754659893295 28.18385653522747 30.12822011209146 20.877168692787777 44-45 20.717067134060695 28.46252786702873 30.061052064820355 20.759352934090217 46-47 21.011171195439854 28.40810383252001 29.789113486805004 20.79161148523513 48-49 20.999346324994658 28.58186196521939 29.473338020265395 20.945453689520555 50-51 21.025122313627893 28.714301106856865 29.181858643141577 21.07871793637366 52-53 21.09759687356469 28.841542424057476 29.070749768837324 20.990110933540507 54-55 20.98881960285184 28.862716545202684 29.172869007541546 20.975594844403933 56-57 20.966775983722744 28.906662717653397 29.11949426477742 21.007067033846443 58-59 20.886872818887834 28.788882400905496 29.363581140151556 20.96066364005511 60-61 20.97722676652682 29.000501722646543 29.228338787480695 20.793932723345943 62-63 20.945894252436695 28.83217986702304 29.342479151188595 20.87944672935167 64-65 20.706484876485565 28.702844485512163 29.35690248929345 21.233768148708823 66-67 20.76775145044642 28.781307137774263 29.19688139887772 21.254060012901597 68-69 20.791993880163467 28.75377799798179 29.27874502062856 21.175483101226177 70-71 20.867707330946118 28.598264991617217 29.290348348295215 21.24367932914145 72-73 20.960314309685078 28.756405901491743 29.117518263111585 21.165761525711595 74-75 20.860380737642924 29.06715688371292 28.971053889622826 21.10140848902133 76-77 20.82411953303824 28.95596948615371 29.060057244172192 21.15985373663586 78-79 20.70048634896662 28.98775505534048 28.998886080252888 21.312872515440006 80-81 20.764366771573854 29.023666878921762 28.946294012968043 21.265672336536337 82-83 20.915967603432648 28.874991705383053 29.10042550159142 21.10861518959288 84-85 20.889065259594542 28.957284111959513 28.81548293629767 21.338167692148275 86-87 20.699261542102494 29.02929609674122 28.999419131652488 21.272023229503798 88-89 20.880881011764473 28.831348616145995 28.864330987411783 21.423439384677746 90-91 20.806047283343247 28.786723488665427 29.082046199992057 21.325183027999266 92-93 20.828416953475926 28.928616616530217 28.807746416478103 21.435220013515753 94-95 20.49060784894758 28.78572898413549 28.97305388291777 21.750609283999157 96-97 20.63211049119839 28.607971595299702 29.025301286736582 21.734616626765323 98-99 20.496317190074702 28.794137577213487 28.89112330524223 21.81842192746958 100-101 20.469872480275157 28.750564279113615 28.873814932141045 21.905748308470187 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 134.0 2 164.5 3 236.5 4 223.0 5 223.5 6 383.0 7 849.0 8 1562.5 9 2098.0 10 2040.0 11 1820.0 12 1963.5 13 2255.5 14 2581.0 15 2959.5 16 3321.0 17 3752.5 18 4227.5 19 4633.0 20 5191.0 21 6025.0 22 7113.0 23 8748.5 24 10978.0 25 14025.0 26 18821.5 27 24528.0 28 30698.0 29 39215.0 30 49485.0 31 59010.0 32 70373.0 33 81690.0 34 90046.5 35 96836.0 36 108065.0 37 121035.5 38 124675.5 39 123312.0 40 120784.5 41 117098.0 42 115675.5 43 117793.5 44 120730.0 45 124216.0 46 123515.5 47 119458.0 48 117133.5 49 113523.0 50 108453.5 51 101629.5 52 93636.0 53 85054.5 54 76343.5 55 65873.5 56 53903.0 57 42815.0 58 33701.0 59 26190.5 60 19135.0 61 13628.5 62 9551.0 63 6079.0 64 3474.0 65 1991.5 66 1088.0 67 593.0 68 320.0 69 168.5 70 97.0 71 56.0 72 39.0 73 30.5 74 19.0 75 14.5 76 8.5 77 3.5 78 3.0 79 3.0 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00424945892946977 2 0.006668381704706406 3 0.001536342843731378 4 0.16693835963693932 5 0.0024516109208479434 6 4.903221841695887E-4 7 3.2688145611305914E-5 8 5.556984753922005E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.7784923769610026E-4 24-25 8.335477130883008E-4 26-27 0.0031870941971023268 28-29 2.1247294647348843E-4 30-31 0.007158703888875994 32-33 0.021950089777991923 34-35 0.014350095923363297 36-37 0.0011767732420070128 38-39 2.941933105017532E-4 40-41 0.002516987212070555 42-43 0.012143646094600146 44-45 0.008711390805413027 46-47 0.011898485002515353 48-49 0.01155525947359664 50-51 0.18932973938068387 52-53 0.11324808047036934 54-55 0.01817460895988609 56-57 0.007224080180098607 58-59 0.018534178561610454 60-61 0.12225366458628413 62-63 0.23471722956198213 64-65 0.24002905322381934 66-67 0.2501460342905185 68-69 0.2450793717207661 70-71 0.23287034933494336 72-73 0.16018825756820462 74-75 0.27194902741325955 76-77 0.2124892905462941 78-79 0.006390532467010306 80-81 3.2688145611305914E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.806443683391774E-5 90-91 0.006096339156508553 92-93 0.01830536154233131 94-95 0.0029255890322118796 96-97 3.2688145611305914E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3059213.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.91164533412298 #Duplication Level Percentage of deduplicated Percentage of total 1 67.10811954577312 28.797198249881422 2 16.372310533484864 14.051255658260564 3 6.383464385628958 8.217748791573452 4 3.074238735533288 5.276825691665087 5 1.7884642720387391 3.837297226723841 6 1.1450451566231366 2.9481462991540406 7 0.7538335812818117 2.264376749664181 8 0.5564242459692624 1.9101663918671838 9 0.39160725174122635 1.5124060347291108 >10 2.1579103396136974 16.706515851249147 >50 0.1704645924668443 5.0640922443491005 >100 0.09316141324122643 7.1852447444665275 >500 0.004041002615579103 1.1194834200711437 >1k 7.624533236117376E-4 0.5741049675668907 >5k 1.5249066472234753E-4 0.5351376787783704 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8626 0.2819679440431248 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7730 0.2526793655753947 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4222 0.13800935077093357 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.2688145611305914E-5 0.0 24-25 0.0 0.0 0.0 3.2688145611305914E-5 0.0 26-27 0.0 0.0 0.0 9.806443683391774E-5 0.0 28-29 0.0 0.0 0.0 5.066662569752417E-4 0.0 30-31 0.0 0.0 0.0 0.002173761683151843 0.0 32-33 0.0 0.0 0.0 0.007338488689738178 0.0 34-35 0.0 0.0 0.0 0.014055902612861545 0.0 36-37 0.0 0.0 0.0 0.026559118309186055 0.0 38-39 0.0 0.0 0.0 0.04654791935049962 0.0 40-41 0.0 0.0 0.0 0.06941327720560811 0.0 42-43 0.0 0.0 0.0 0.09309583870099924 0.0 44-45 0.0 0.0 0.0 0.12272764269764806 0.0 46-47 0.0 0.0 0.0 0.15986137611209156 0.0 48-49 0.0 0.0 0.0 0.20157144991211792 0.0 50-51 0.0 0.0 0.0 0.24980280876159977 0.0 52-53 0.0 0.0 0.0 0.29845911350402865 0.0 54-55 0.0 0.0 0.0 0.35275412336440776 0.0 56-57 0.0 0.0 0.0 0.4196994455763623 0.0 58-59 0.0 0.0 0.0 0.5001449719257861 0.0 60-61 0.0 0.0 0.0 0.5850360860783477 0.0 62-63 0.0 0.0 0.0 0.6738170895586544 0.0 64-65 0.0 0.0 0.0 0.7691193780884169 0.0 66-67 0.0 0.0 0.0 0.879409181380963 0.0 68-69 0.0 0.0 0.0 1.0099166027341018 0.0 70-71 0.0 0.0 0.0 1.1565392798736145 0.0 72-73 0.0 0.0 0.0 1.3134914110263 0.0 74-75 0.0 0.0 0.0 1.4740555822690347 0.0 76-77 0.0 0.0 0.0 1.655017156373224 0.0 78-79 0.0 0.0 0.0 1.8709713903543166 0.0 80-81 0.0 0.0 0.0 2.1091535633510974 0.0 82-83 0.0 0.0 0.0 2.3587275550934175 0.0 84-85 0.0 0.0 0.0 2.611668425833703 0.0 86-87 0.0 0.0 0.0 2.8844183128144394 0.0 88-89 0.0 0.0 0.0 3.190657204973959 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4340 0.0 61.325516 1 GTATCAA 8805 0.0 44.26163 1 ATCAACG 10910 0.0 35.15077 3 TCAACGC 11235 0.0 34.303135 4 CAACGCA 11305 0.0 34.03613 5 TATCAAC 11550 0.0 33.246887 2 AACGCAG 11765 0.0 32.905907 6 ACGCAGA 13565 0.0 28.154755 7 CGCAGAG 13685 0.0 27.803867 8 GCAGAGT 15110 0.0 24.67935 9 GTACATG 8875 0.0 23.56282 1 GTGGTAT 2155 0.0 23.377686 1 ACATGGG 8920 0.0 22.00246 3 TACATGG 9330 0.0 21.90324 2 GAGTACT 10400 0.0 21.80571 12-13 AGAGTAC 14140 0.0 21.440115 10-11 CAGAGTA 14580 0.0 21.118488 10-11 TGGTATC 2175 0.0 20.75802 2 ACGTCCG 115 0.0037504956 20.62756 6 GTACTTT 11325 0.0 19.815207 14-15 >>END_MODULE