FastQCFastQC Report
Sat 18 Jun 2016
SRR1930998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806006
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1248054.447780938458435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.061.6100351
GTATCAA46900.049.0797351
ATCAACG59000.038.349533
TCAACGC59950.037.571694
TATCAAC61050.036.9413452
CAACGCA60900.036.3119745
AACGCAG67850.032.2060366
ACGCAGA75500.028.649757
CGCAGAG75300.027.7369068
GTACATG51250.026.808321
TACATGG53350.025.2677782
GCAGAGT82200.024.6761959
ACATGGG55350.024.126483
GAGTACT50250.023.46496412-13
GTACTAG5350.022.9977931
CAGAGTA78300.021.43676210-11
GTACTTT56300.021.34060914-15
AGAGTAC72700.021.32412710-11
GTGTAGC9750.018.9289531
AGTACTT56800.018.85649712-13
Produced by FastQC (version 0.11.5)