##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930998_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2806006 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.633258089968447 28.0 2.0 31.0 2.0 33.0 2 19.40847667467568 27.0 2.0 31.0 2.0 33.0 3 20.01018957193962 27.0 2.0 31.0 2.0 33.0 4 21.54616205382312 30.0 2.0 35.0 2.0 37.0 5 20.927265658020687 28.0 2.0 35.0 2.0 37.0 6 21.82515575519083 30.0 2.0 35.0 2.0 37.0 7 21.96418076083943 29.0 2.0 35.0 2.0 37.0 8 22.350922271727146 30.0 2.0 35.0 2.0 37.0 9 23.536746535823514 32.0 2.0 37.0 2.0 39.0 10-11 23.84523874859854 32.0 2.0 37.0 2.0 39.0 12-13 23.716623022188834 32.0 2.0 37.0 2.0 39.0 14-15 23.66782162974705 32.0 2.0 38.0 2.0 40.0 16-17 22.57074788863602 30.0 2.0 37.5 2.0 40.0 18-19 21.586194042350584 27.0 2.0 37.0 2.0 39.5 20-21 21.26149997540989 27.0 2.0 37.0 2.0 39.5 22-23 20.89970477611238 27.0 2.0 37.0 2.0 39.0 24-25 20.727620860397305 26.5 2.0 37.0 2.0 39.0 26-27 20.42656038511678 25.0 2.0 36.0 2.0 39.0 28-29 20.214102357585837 25.0 2.0 36.0 2.0 39.0 30-31 20.15881095763872 25.0 2.0 36.0 2.0 39.0 32-33 19.999974518942583 24.5 2.0 36.0 2.0 39.0 34-35 19.656354975719935 24.0 2.0 36.0 2.0 39.0 36-37 19.227580767824445 22.5 2.0 35.0 2.0 39.0 38-39 19.06102321235236 21.5 2.0 35.0 2.0 38.0 40-41 18.889729743984866 20.5 2.0 35.0 2.0 38.0 42-43 18.72911444237824 20.0 2.0 35.0 2.0 38.0 44-45 18.33321186768667 18.0 2.0 34.5 2.0 38.0 46-47 17.977620325829665 15.0 2.0 34.0 2.0 38.0 48-49 17.696306779101683 14.5 2.0 34.0 2.0 38.0 50-51 15.549538026647127 7.0 2.0 31.5 2.0 37.5 52-53 17.334586597462728 14.5 2.0 33.5 2.0 37.5 54-55 18.051904913959557 15.0 2.0 35.0 2.0 38.5 56-57 18.001838734485958 14.5 2.0 35.0 2.0 39.0 58-59 17.716324733446758 12.0 2.0 34.5 2.0 38.5 60-61 17.414694943631623 8.5 2.0 34.0 2.0 38.0 62-63 16.974529099367572 8.0 2.0 34.0 2.0 38.0 64-65 16.602236595360097 6.5 2.0 33.5 2.0 38.0 66-67 16.22893732230081 2.0 2.0 33.0 2.0 38.0 68-69 15.77976044955 2.0 2.0 32.0 2.0 37.0 70-71 15.425504792220686 2.0 2.0 32.0 2.0 36.5 72-73 15.020746570035845 2.0 2.0 31.0 2.0 36.0 74-75 14.565929830513548 2.0 2.0 31.0 2.0 35.5 76-77 14.139877641031417 2.0 2.0 30.5 2.0 35.0 78-79 13.752941191144995 2.0 2.0 30.0 2.0 34.5 80-81 13.316581468464431 2.0 2.0 29.5 2.0 34.0 82-83 12.855439902836986 2.0 2.0 29.0 2.0 34.0 84-85 12.44748211514872 2.0 2.0 29.0 2.0 34.0 86-87 11.96456885694471 2.0 2.0 27.5 2.0 34.0 88-89 11.540181489276929 2.0 2.0 26.5 2.0 34.0 90-91 11.054659540998843 2.0 2.0 26.0 2.0 33.5 92-93 10.519979287285913 2.0 2.0 25.0 2.0 33.0 94-95 9.971608756360464 2.0 2.0 23.5 2.0 32.0 96-97 9.352629680763334 2.0 2.0 19.0 2.0 32.0 98-99 8.711225670935843 2.0 2.0 4.5 2.0 32.0 100-101 7.855873259002298 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.970090866125307 1101 2 9.454978485428633 1101 3 8.755535319525809 1101 4 10.605682515147834 1101 5 11.288831798132552 1101 6 10.873561522425828 1101 7 10.409273783364323 1101 8 9.898960107290044 1101 9 9.647395265048363 1101 10-11 9.355321510454985 1101 12-13 9.339604204131692 1101 14-15 9.803362886437256 1101 16-17 10.658749528535363 1101 18-19 11.53682905012612 1101 20-21 11.57083520529519 1101 22-23 11.706131484612154 1101 24-25 11.441893004439212 1101 26-27 11.696472080916624 1101 28-29 11.743160528014634 1101 30-31 11.289811995506913 1101 32-33 11.41043155852173 1101 34-35 11.13197966026167 1101 36-37 11.658621319141915 1101 38-39 11.506532305194106 1101 40-41 11.221012460855711 1101 42-43 10.750844390271048 1101 44-45 10.622780005183746 1101 46-47 10.546650416418316 1101 48-49 10.610411740574786 1101 50-51 11.917215722461448 1101 52-53 11.220325839224525 1101 54-55 11.012414171336527 1101 56-57 11.166199657758153 1101 58-59 10.966020204968729 1101 60-61 10.630535951931854 1101 62-63 10.626274494347356 1101 64-65 10.478637442552095 1101 66-67 10.054346186293063 1101 68-69 10.071381829493816 1101 70-71 9.667139681019522 1101 72-73 9.645338616083256 1101 74-75 9.398397697005095 1101 76-77 9.325857656095566 1101 78-79 8.819023874071917 1101 80-81 8.707719271744574 1101 82-83 8.511701265584199 1101 84-85 8.310711186292359 1101 86-87 8.044978519868243 1101 88-89 7.64803215767644 1101 90-91 7.226141238200979 1101 92-93 7.1080999416724 1101 94-95 6.74463702932403 1101 96-97 6.15332394703869 1101 98-99 5.939682936260107 1101 100-101 5.1857556628674635 1102 1 7.834628370414631 1102 2 9.151373028317451 1102 3 8.584543231664579 1102 4 10.5033946847708 1102 5 11.176594707893031 1102 6 10.320079677031451 1102 7 10.128233274321385 1102 8 9.742964098465158 1102 9 9.51927753032702 1102 10-11 8.839806076790467 1102 12-13 8.977925282599433 1102 14-15 9.44492011965103 1102 16-17 10.127771196185986 1102 18-19 11.127003117673492 1102 20-21 11.17604249719981 1102 22-23 11.28554811889255 1102 24-25 11.250968218213313 1102 26-27 11.421402512242405 1102 28-29 11.14617106665516 1102 30-31 11.132032516831917 1102 32-33 11.116722084623085 1102 34-35 10.798640517177645 1102 36-37 10.847505337729164 1102 38-39 10.895777813885989 1102 40-41 10.389170997332538 1102 42-43 10.056920289348511 1102 44-45 10.035601111340501 1102 46-47 10.0580877759336 1102 48-49 9.64532370678231 1102 50-51 11.220093971842646 1102 52-53 10.753656131709622 1102 54-55 10.255258845303207 1102 56-57 10.173592318287746 1102 58-59 10.212040154910923 1102 60-61 9.933030695961659 1102 62-63 10.060803795763157 1102 64-65 9.946042454364427 1102 66-67 9.712571285207162 1102 68-69 9.579199652523371 1102 70-71 9.006788398770462 1102 72-73 8.903650885980047 1102 74-75 8.67376529352854 1102 76-77 8.395156918578616 1102 78-79 8.006143953460306 1102 80-81 7.635144731407104 1102 82-83 7.718276593081081 1102 84-85 7.5449918551009745 1102 86-87 7.164281441258117 1102 88-89 6.888663254824369 1102 90-91 6.573594181692625 1102 92-93 6.403648634305437 1102 94-95 6.000246714450375 1102 96-97 5.800836236515574 1102 98-99 5.480919434152282 1102 100-101 4.68091972528248 1103 1 7.844092236406954 1103 2 9.089943834061803 1103 3 8.60781994378275 1103 4 10.663019190391086 1103 5 11.177355689338103 1103 6 10.397528635666294 1103 7 10.037990795558844 1103 8 9.852987650936967 1103 9 9.47412043978392 1103 10-11 9.010125998866727 1103 12-13 8.870650078992458 1103 14-15 9.417814059063161 1103 16-17 10.332243943898302 1103 18-19 11.108798534249583 1103 20-21 11.465426289592152 1103 22-23 11.453946550809572 1103 24-25 11.289615650561512 1103 26-27 11.342537943158959 1103 28-29 11.092136760035846 1103 30-31 11.062805124382397 1103 32-33 11.169494394314668 1103 34-35 10.973754766483143 1103 36-37 11.113192292830536 1103 38-39 10.757161008158448 1103 40-41 10.467856130450247 1103 42-43 10.311201230342423 1103 44-45 10.167946065574721 1103 46-47 10.041689922919762 1103 48-49 9.967906515759875 1103 50-51 11.561359740048372 1103 52-53 10.831097583819798 1103 54-55 10.579824757994519 1103 56-57 10.157423324198085 1103 58-59 10.091235851810673 1103 60-61 9.99968305018795 1103 62-63 9.731325313065994 1103 64-65 9.579121846972484 1103 66-67 9.47433677176808 1103 68-69 9.315406563570455 1103 70-71 8.851292842032544 1103 72-73 8.815439612741349 1103 74-75 8.43387616189532 1103 76-77 8.269007849808043 1103 78-79 7.92949987442681 1103 80-81 7.4768610712496955 1103 82-83 7.145761883295334 1103 84-85 6.838489703430739 1103 86-87 6.7542019201383585 1103 88-89 6.299950305528842 1103 90-91 6.198793645066191 1103 92-93 6.045892112336542 1103 94-95 5.797178827843336 1103 96-97 5.480387502475946 1103 98-99 4.895533496695444 1103 100-101 4.431584683263957 1104 1 7.888531625162393 1104 2 9.358158129433967 1104 3 8.688243963243625 1104 4 10.643029936669567 1104 5 11.291834359621351 1104 6 10.631187179134049 1104 7 10.241094400735399 1104 8 9.800578027256588 1104 9 9.811222730481337 1104 10-11 9.22833341615037 1104 12-13 9.149421828742906 1104 14-15 9.453740902067565 1104 16-17 10.44152613011931 1104 18-19 11.204686613052484 1104 20-21 11.281841112402013 1104 22-23 11.415371467361307 1104 24-25 11.290378001433382 1104 26-27 11.277201451783611 1104 28-29 11.216663494636865 1104 30-31 10.862082572364649 1104 32-33 10.987063854190719 1104 34-35 10.821480823386686 1104 36-37 10.875246819024294 1104 38-39 10.410375191835158 1104 40-41 10.419008357246103 1104 42-43 10.041329962293318 1104 44-45 10.240817591111242 1104 46-47 10.010190041081792 1104 48-49 9.815365150230033 1104 50-51 11.574796291739263 1104 52-53 10.568299518620218 1104 54-55 10.471203279781598 1104 56-57 10.179475945690406 1104 58-59 10.29301516040405 1104 60-61 10.13332777869157 1104 62-63 10.052461796681449 1104 64-65 9.577377281544226 1104 66-67 9.607827066608806 1104 68-69 9.534727055453722 1104 70-71 9.101091496278672 1104 72-73 8.829814817761294 1104 74-75 8.694687458891185 1104 76-77 8.327996041834867 1104 78-79 8.047953668368685 1104 80-81 7.613973355750952 1104 82-83 7.297251724299793 1104 84-85 6.90933830670847 1104 86-87 6.549959374046351 1104 88-89 6.425904684402177 1104 90-91 5.883906404567062 1104 92-93 5.3940849386665715 1104 94-95 5.181780903967903 1104 96-97 4.7518497840966365 1104 98-99 4.375831946950404 1104 100-101 3.79352769036426 1105 1 7.814748502901587 1105 2 8.899034209992834 1105 3 8.440159396014291 1105 4 10.56805467218101 1105 5 10.305700067966924 1105 6 10.438727392337459 1105 7 9.99157592253087 1105 8 9.635875458103701 1105 9 9.76712026384116 1105 10-11 9.026886892655952 1105 12-13 8.74028880985868 1105 14-15 9.537039910919361 1105 16-17 10.519161426743874 1105 18-19 10.79032982273706 1105 20-21 11.151278732264132 1105 22-23 11.532490917708873 1105 24-25 11.380956334392373 1105 26-27 11.653195969081189 1105 28-29 11.38378006541383 1105 30-31 11.053552881112207 1105 32-33 11.000482019673886 1105 34-35 11.013594658571584 1105 36-37 10.909563180168522 1105 38-39 10.595680743071359 1105 40-41 10.358063742906992 1105 42-43 10.121841661988661 1105 44-45 10.057349033142607 1105 46-47 9.797978703636435 1105 48-49 9.630360917261253 1105 50-51 10.971725114513214 1105 52-53 10.21077854094841 1105 54-55 10.054795140963257 1105 56-57 10.01458067567307 1105 58-59 9.79002184676698 1105 60-61 9.444655779060156 1105 62-63 9.442958061878379 1105 64-65 9.315961915003687 1105 66-67 8.919243815343236 1105 68-69 8.788541277076995 1105 70-71 8.704947759632683 1105 72-73 8.540497967090067 1105 74-75 7.9610047760528495 1105 76-77 7.647006794151482 1105 78-79 7.406196842346581 1105 80-81 7.281854913253094 1105 82-83 7.058327128735483 1105 84-85 6.5121446210308775 1105 86-87 6.514057060599329 1105 88-89 6.1804429452567184 1105 90-91 5.738340885697239 1105 92-93 5.65533496026335 1105 94-95 5.382967344645868 1105 96-97 4.82160150751964 1105 98-99 4.372320195422791 1105 100-101 3.813371675229237 1106 1 7.5804532130056295 1106 2 8.63686512470542 1106 3 8.262534534248058 1106 4 10.081502504449848 1106 5 10.709569485015379 1106 6 10.082388499561723 1106 7 9.328984349712705 1106 8 8.94832259074041 1106 9 8.774973973019883 1106 10-11 8.290783392238431 1106 12-13 8.27164045399418 1106 14-15 8.637574678122412 1106 16-17 9.614341976970692 1106 18-19 10.834941301217736 1106 20-21 10.737414618878596 1106 22-23 10.797701935786467 1106 24-25 10.706398118228613 1106 26-27 10.857247741858732 1106 28-29 10.704923286881254 1106 30-31 10.300668365551036 1106 32-33 10.632255925652355 1106 34-35 10.52520369962419 1106 36-37 10.48584835925178 1106 38-39 10.060590806489625 1106 40-41 10.03918225156212 1106 42-43 9.544353567019243 1106 44-45 9.181412654528781 1106 46-47 9.069519022758957 1106 48-49 9.012149831477323 1106 50-51 11.00316996770331 1106 52-53 10.362293244507509 1106 54-55 9.739315103047712 1106 56-57 9.545611391780408 1106 58-59 9.26456990698048 1106 60-61 8.938205735051039 1106 62-63 8.90757416695861 1106 64-65 8.764737220246413 1106 66-67 8.532210932633177 1106 68-69 8.135253457446666 1106 70-71 7.737240183293791 1106 72-73 7.585613406391337 1106 74-75 7.431814205288443 1106 76-77 7.173962604483469 1106 78-79 6.787484729683236 1106 80-81 6.544817291099582 1106 82-83 6.341027595909194 1106 84-85 5.930096165863759 1106 86-87 5.7193943509918 1106 88-89 5.408792264808049 1106 90-91 5.227376296110473 1106 92-93 4.958234709749911 1106 94-95 4.855882102370034 1106 96-97 4.34200684277884 1106 98-99 3.9338289182556245 1106 100-101 3.5909608783256752 1107 1 7.088313736108738 1107 2 7.993627759993402 1107 3 7.958574064818073 1107 4 9.565090673499654 1107 5 10.286480797542115 1107 6 9.326286418812693 1107 7 9.501700852904069 1107 8 7.8278353530667 1107 9 5.484740561900704 1107 10-11 7.436131512919911 1107 12-13 7.450238451209611 1107 14-15 7.866337959095425 1107 16-17 8.758352846448545 1107 18-19 10.023875617278321 1107 20-21 10.360733426531432 1107 22-23 9.712491940796554 1107 24-25 10.022366944948363 1107 26-27 10.00299282933878 1107 28-29 10.178901756009381 1107 30-31 10.153409535160794 1107 32-33 10.288240434570039 1107 34-35 10.31341800543241 1107 36-37 10.262024501770451 1107 38-39 10.146956760759405 1107 40-41 9.6614306107809 1107 42-43 9.582259877496398 1107 44-45 8.95914864436929 1107 46-47 9.078772278645811 1107 48-49 9.199731490986085 1107 50-51 10.482560067735372 1107 52-53 9.624767446130917 1107 54-55 9.374922913246678 1107 56-57 9.165094379364145 1107 58-59 9.211292407987408 1107 60-61 8.976184272052006 1107 62-63 8.865056187354398 1107 64-65 9.060378243483036 1107 66-67 9.083155079538578 1107 68-69 8.79338980326235 1107 70-71 8.367579141142079 1107 72-73 7.98357530115093 1107 74-75 7.934282521622311 1107 76-77 7.540709803166022 1107 78-79 7.2049612934600376 1107 80-81 6.956110616966905 1107 82-83 6.86917015298266 1107 84-85 6.748940992479916 1107 86-87 6.432441832507616 1107 88-89 6.217900799728177 1107 90-91 6.027035994201368 1107 92-93 5.680604771219482 1107 94-95 5.540600488139601 1107 96-97 5.111672327922282 1107 98-99 4.826463018462727 1107 100-101 4.493875034367117 1108 1 6.598533530300166 1108 2 7.849157461102092 1108 3 7.731527398326314 1108 4 9.084903297151968 1108 5 10.734561794389126 1108 6 9.48702584702355 1108 7 8.610974683786388 1108 8 5.7861115902288915 1108 9 6.905271100532026 1108 10-11 6.042402875633137 1108 12-13 4.779300250961651 1108 14-15 7.064556188565859 1108 16-17 8.234197382067475 1108 18-19 7.8312366675653315 1108 20-21 7.735744105785827 1108 22-23 8.665293336263975 1108 24-25 8.564910739982022 1108 26-27 6.946271693429377 1108 28-29 8.90960930454365 1108 30-31 9.263653748686682 1108 32-33 9.569072366987012 1108 34-35 9.15576076139984 1108 36-37 8.864867907911073 1108 38-39 8.659038613292193 1108 40-41 8.76422221833327 1108 42-43 8.230753312193514 1108 44-45 7.569701967652353 1108 46-47 8.448188542191648 1108 48-49 9.014433813917321 1108 50-51 10.443909407505183 1108 52-53 9.792258517601535 1108 54-55 9.22795484606727 1108 56-57 9.197641383219437 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-15.468452729504207 2313 60-61 -15.164157780261878 2313 62-63 -14.73839787032501 2313 64-65 -14.376241474826823 2313 66-67 -14.009400252453375 2313 68-69 -13.572136689024703 2313 70-71 -13.202340375960537 2313 72-73 -12.810857965113323 2313 74-75 -12.357300308692913 2313 76-77 -11.946044053306144 2313 78-79 -11.549565869517828 2313 80-81 -11.135941696916396 2313 82-83 -10.678113549230618 2313 84-85 -10.26514351456234 2313 86-87 -9.794587007197281 2313 88-89 -9.37582824118396 2313 90-91 -8.890133690573949 2313 92-93 -8.364834531262073 2313 94-95 -7.818219096032097 2313 96-97 -7.209923916209174 2313 98-99 -6.60412048254331 2313 100-101 -5.772221545109745 2314 1 -18.348641327606888 2314 2 -17.1297793493292 2314 3 -17.718823654833166 2314 4 -19.239402954937635 2314 5 -18.57904569474494 2314 6 -19.476865828001525 2314 7 -19.627407128316587 2314 8 -20.038361830031892 2314 9 -21.20338821073511 2314 10-11 -21.52413005649659 2314 12-13 -21.413778986751492 2314 14-15 -21.37024822467386 2314 16-17 -20.26931314952731 2314 18-19 -19.27424644844938 2314 20-21 -18.955161945701967 2314 22-23 -18.584288743308075 2314 24-25 -18.394467394421188 2314 26-27 -18.12510911566457 2314 28-29 -17.92706739221329 2314 30-31 -17.87543016973525 2314 32-33 -17.71528069219052 2314 34-35 -17.359117205835197 2314 36-37 -16.948572413051817 2314 38-39 -16.782285358623557 2314 40-41 -16.621936257093008 2314 42-43 -16.45731088038422 2314 44-45 -16.078074695670953 2314 46-47 -15.707445025177126 2314 48-49 -15.436418743755699 2314 50-51 -13.362342682096957 2314 52-53 -15.101255357356674 2314 54-55 -15.800798079375724 2314 56-57 -15.740673561615065 2314 58-59 -15.468452729504207 2314 60-61 -15.164157780261878 2314 62-63 -14.73839787032501 2314 64-65 -14.376241474826823 2314 66-67 -14.009400252453375 2314 68-69 -13.572136689024703 2314 70-71 -13.202340375960537 2314 72-73 -12.810857965113323 2314 74-75 -12.357300308692913 2314 76-77 -11.946044053306144 2314 78-79 -11.549565869517828 2314 80-81 -11.135941696916396 2314 82-83 -10.678113549230618 2314 84-85 -10.26514351456234 2314 86-87 -9.794587007197281 2314 88-89 -9.37582824118396 2314 90-91 -8.890133690573949 2314 92-93 -8.364834531262073 2314 94-95 -7.818219096032097 2314 96-97 -7.209923916209174 2314 98-99 -6.60412048254331 2314 100-101 -5.772221545109745 2315 1 -18.348641327606888 2315 2 -17.1297793493292 2315 3 -17.718823654833166 2315 4 -19.239402954937635 2315 5 -18.57904569474494 2315 6 -19.476865828001525 2315 7 -19.627407128316587 2315 8 -20.038361830031892 2315 9 -21.20338821073511 2315 10-11 -21.52413005649659 2315 12-13 -21.413778986751492 2315 14-15 -21.37024822467386 2315 16-17 -20.26931314952731 2315 18-19 -19.27424644844938 2315 20-21 -18.955161945701967 2315 22-23 -18.584288743308075 2315 24-25 -18.394467394421188 2315 26-27 -18.12510911566457 2315 28-29 -17.92706739221329 2315 30-31 -17.87543016973525 2315 32-33 -17.71528069219052 2315 34-35 -17.359117205835197 2315 36-37 -16.948572413051817 2315 38-39 -16.782285358623557 2315 40-41 -16.621936257093008 2315 42-43 -16.45731088038422 2315 44-45 -16.078074695670953 2315 46-47 -15.707445025177126 2315 48-49 -15.436418743755699 2315 50-51 -13.362342682096957 2315 52-53 -15.101255357356674 2315 54-55 -15.800798079375724 2315 56-57 -15.740673561615065 2315 58-59 -15.468452729504207 2315 60-61 -15.164157780261878 2315 62-63 -14.73839787032501 2315 64-65 -14.376241474826823 2315 66-67 -14.009400252453375 2315 68-69 -13.572136689024703 2315 70-71 -13.202340375960537 2315 72-73 -12.810857965113323 2315 74-75 -12.357300308692913 2315 76-77 -11.946044053306144 2315 78-79 -11.549565869517828 2315 80-81 -11.135941696916396 2315 82-83 -10.678113549230618 2315 84-85 -10.26514351456234 2315 86-87 -9.794587007197281 2315 88-89 -9.37582824118396 2315 90-91 -8.890133690573949 2315 92-93 -8.364834531262073 2315 94-95 -7.818219096032097 2315 96-97 -7.209923916209174 2315 98-99 -6.60412048254331 2315 100-101 -5.772221545109745 2316 1 -18.348641327606888 2316 2 -17.1297793493292 2316 3 -17.718823654833166 2316 4 -19.239402954937635 2316 5 -18.57904569474494 2316 6 -19.476865828001525 2316 7 -19.627407128316587 2316 8 -20.038361830031892 2316 9 -21.20338821073511 2316 10-11 -21.52413005649659 2316 12-13 -21.413778986751492 2316 14-15 -21.37024822467386 2316 16-17 -20.26931314952731 2316 18-19 -19.27424644844938 2316 20-21 -18.955161945701967 2316 22-23 -18.584288743308075 2316 24-25 -18.394467394421188 2316 26-27 -18.12510911566457 2316 28-29 -17.92706739221329 2316 30-31 -17.87543016973525 2316 32-33 -17.71528069219052 2316 34-35 -17.359117205835197 2316 36-37 -16.948572413051817 2316 38-39 -16.782285358623557 2316 40-41 -16.621936257093008 2316 42-43 -16.45731088038422 2316 44-45 -16.078074695670953 2316 46-47 -15.707445025177126 2316 48-49 -15.436418743755699 2316 50-51 -13.362342682096957 2316 52-53 -15.101255357356674 2316 54-55 -15.800798079375724 2316 56-57 -15.740673561615065 2316 58-59 -15.468452729504207 2316 60-61 -15.164157780261878 2316 62-63 -14.73839787032501 2316 64-65 -14.376241474826823 2316 66-67 -14.009400252453375 2316 68-69 -13.572136689024703 2316 70-71 -13.202340375960537 2316 72-73 -12.810857965113323 2316 74-75 -12.357300308692913 2316 76-77 -11.946044053306144 2316 78-79 -11.549565869517828 2316 80-81 -11.135941696916396 2316 82-83 -10.678113549230618 2316 84-85 -10.26514351456234 2316 86-87 -9.794587007197281 2316 88-89 -9.37582824118396 2316 90-91 -8.890133690573949 2316 92-93 -8.364834531262073 2316 94-95 -7.818219096032097 2316 96-97 -7.209923916209174 2316 98-99 -6.60412048254331 2316 100-101 -5.772221545109745 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 754603.0 3 31375.0 4 105774.0 5 58438.0 6 37546.0 7 36641.0 8 35152.0 9 35687.0 10 38338.0 11 39952.0 12 40894.0 13 41135.0 14 42036.0 15 42734.0 16 44742.0 17 44657.0 18 44196.0 19 45466.0 20 46956.0 21 50061.0 22 52774.0 23 55769.0 24 59231.0 25 62922.0 26 66737.0 27 70840.0 28 75776.0 29 81128.0 30 86979.0 31 93021.0 32 98347.0 33 102405.0 34 102007.0 35 91770.0 36 63276.0 37 24013.0 38 2616.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32713259610959 20.55613449905174 14.033315024340865 23.0834178804978 2 14.945083451049504 20.66374256167545 41.862191584896195 22.52898240237885 3 18.728225339051814 26.244720064912485 29.98270545960357 25.044349136432132 4 12.174929129432979 17.511764589936256 39.54180895953567 30.771497321095094 5 12.657801405311753 38.02322371281123 35.69016926993811 13.62880561193891 6 29.465602442677092 38.70335701328597 18.22458963510891 13.606450908928036 7 26.23806321073564 33.2096366030852 22.961140972001736 17.59115921417742 8 24.389038576215576 36.87079101959296 20.63747919901199 18.102691205179475 9 25.278447483079127 17.128712751112516 21.646866810888753 35.94597295491961 10-11 23.925447403580915 27.436532224179473 28.866560712055506 19.771459660184103 12-13 24.637475712525884 24.921710725488005 29.206531363138645 21.234282198847467 14-15 21.916351273362274 26.55137171920407 26.883398589802482 24.64887841763118 16-17 21.142700021885652 29.577810915376713 27.723859627647148 21.55562943509049 18-19 20.775380898407935 28.448834715584038 30.044884938786044 20.730899447221987 20-21 21.55938130409851 27.635657529274177 30.14201732159228 20.66294384503503 22-23 21.299052069599654 27.65556159545426 29.790532308619472 21.254854026326615 24-25 21.13918077333683 27.871454538546107 30.032762692938924 20.956601995178136 26-27 21.06041238815416 28.10713186865415 29.773519207342243 21.058936535849455 28-29 20.927788533895686 28.12074881131637 29.98308580135664 20.96837685343131 30-31 21.229904156248377 27.973227446838727 29.855989477640353 20.94087891927254 32-33 21.10294189038303 28.1445943977863 29.6652507403272 21.087212971503472 34-35 21.213333626548454 28.161538830785794 29.766773382782645 20.85835415988311 36-37 21.274357491177483 28.021996017155733 29.621166844828394 21.082479646838387 38-39 21.269117732301797 28.19329798688013 29.709594231103647 20.82799004971443 40-41 21.334209418659434 28.044907742198788 29.736575133278116 20.88430770586367 42-43 21.224460846074255 28.0489914690971 29.467749487335915 21.258798197492727 44-45 21.22758630759698 28.407692573998716 29.346149108798357 21.018572009605943 46-47 21.393671268848934 28.28455847516309 28.96035727407133 21.361412981916644 48-49 21.43072937297015 28.345094102746398 28.874079859949575 21.350096664333883 50-51 21.621577091640027 28.543708079576536 28.50833440677577 21.326380422007666 52-53 21.07029285905016 28.875203415688922 28.614455761982686 21.440047963278232 54-55 21.063984600693786 28.76751867454046 28.69868379435893 21.46981293040682 56-57 21.07529961068659 28.72648186989005 28.836993714474495 21.361224804948865 58-59 21.01471391529655 28.72782867521725 28.814559395863586 21.442898013622617 60-61 21.067666055383523 28.919732554015958 28.769692467239327 21.24290892336119 62-63 21.01787671903495 28.71282316542158 28.76346882122412 21.505831294319353 64-65 20.82884019123857 28.650200999492835 28.8388784196055 21.682080389663096 66-67 20.811039822880627 28.702496247225266 28.576611664609157 21.90985226528495 68-69 20.859307397946 28.671750145392128 28.62815599565186 21.840786461010012 70-71 21.016351139965085 28.45842602863286 28.685016670761726 21.840206160640328 72-73 20.96411896351028 28.630858574063485 28.59872342068823 21.806299041738 74-75 20.95276668813908 28.836939122047017 28.319069833747573 21.89122435606633 76-77 20.895987261685033 28.71429260236709 28.45851626875048 21.9312038671974 78-79 20.77447863807608 28.65716042578088 28.50414669251019 22.064214243632847 80-81 20.84632948164174 28.78168737844914 28.26066596305609 22.111317176853028 82-83 21.07479570994946 28.503703744868 28.474621168179493 21.946879377003047 84-85 21.075534947069265 28.555496492477104 28.240764762695214 22.12820379775842 86-87 20.909656024661587 28.596301443505862 28.41569571022773 22.078346821604825 88-89 21.10717011025603 28.518693397841428 28.233173480371203 22.140963011531344 90-91 21.037243950817853 28.47009684276292 28.395359665853075 22.097299540566144 92-93 20.98278045114932 28.481124267029305 28.209712058858706 22.32638322296267 94-95 20.811914458892243 28.340161586417967 28.368571843081202 22.479352111608584 96-97 20.86317154783581 28.335288632193244 28.277381704418975 22.52415811555197 98-99 20.73792276047174 28.40781740190482 28.00776048930781 22.846499348315632 100-101 20.975186527010546 28.479401320037308 27.853257945333237 22.692154207618913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 169329.0 1 153879.5 2 102367.0 3 51648.5 4 31850.5 5 24948.5 6 23037.0 7 23486.0 8 24350.0 9 24295.0 10 22466.0 11 19143.0 12 15355.5 13 11718.0 14 8910.5 15 7194.5 16 6568.5 17 6845.0 18 7888.0 19 9233.0 20 10845.0 21 12675.5 22 14448.0 23 16281.0 24 18347.5 25 20782.0 26 23455.5 27 26432.0 28 29900.5 29 33976.0 30 38310.5 31 43201.0 32 49098.0 33 54723.0 34 59749.0 35 64955.0 36 71022.5 37 76788.5 38 79835.0 39 80969.0 40 81077.0 41 80684.0 42 80444.5 43 81184.0 44 82407.5 45 82826.0 46 82278.5 47 80844.5 48 78860.5 49 75826.0 50 71474.0 51 66783.5 52 62114.0 53 56741.5 54 50851.5 55 44141.0 56 37017.5 57 30341.0 58 24340.5 59 19307.0 60 14779.0 61 10997.0 62 8160.0 63 5818.0 64 3938.0 65 2616.0 66 1734.5 67 1058.5 68 646.5 69 391.0 70 217.0 71 137.0 72 110.0 73 79.5 74 48.5 75 27.0 76 18.0 77 14.0 78 7.5 79 7.0 80 4.0 81 1.5 82 3.0 83 1.5 84 1.0 85 1.0 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.335982175376675 2 29.38500487882064 3 23.138083097470215 4 29.486715281435604 5 32.61504073761781 6 25.20753697604353 7 24.12232903279608 8 20.09707035551599 9 11.915690843141462 10-11 15.564916824839292 12-13 15.655793323321475 14-15 19.815976872465704 16-17 23.14114795192883 18-19 27.13228339497492 20-21 29.21230389386195 22-23 28.801791585620272 24-25 27.310650796897797 26-27 28.76547662406994 28-29 25.71849454348993 30-31 27.602310900261795 32-33 26.589608147666112 34-35 24.644191780060343 36-37 27.638839688867378 38-39 27.759331234501992 40-41 24.771882169888446 42-43 25.636331497509275 44-45 24.338704193790033 46-47 27.63785964819747 48-49 23.029619323693534 50-51 28.620893896876915 52-53 22.19057977780518 54-55 23.324273005831063 56-57 25.458783765964867 58-59 24.743264982327194 60-61 24.277941672255867 62-63 24.1091608499768 64-65 22.6337719876579 66-67 23.138528570501986 68-69 24.136797996868147 70-71 22.618964464081685 72-73 22.480992556680206 74-75 19.75332554527681 76-77 22.02281463403856 78-79 21.149616216073667 80-81 22.1847886283921 82-83 23.044765406773898 84-85 19.98429440279173 86-87 21.5439845816438 88-89 20.372764705421158 90-91 21.511767971985805 92-93 21.486037449670455 94-95 20.533723021262247 96-97 19.439801625513272 98-99 18.84636027150334 100-101 20.519058049056202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2806006.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.95238193977333 #Duplication Level Percentage of deduplicated Percentage of total 1 87.62091461680939 64.79775343654556 2 7.823350750501949 11.57110845499865 3 2.177743552793015 4.831479689490839 4 0.9091124639728171 2.6892412864770474 5 0.4518743595468131 1.6708592612998192 6 0.2553718944600758 1.1331215925477007 7 0.1591194468163092 0.8237083473503604 8 0.11868840098092659 0.7021831968929961 9 0.07490270045181067 0.4985309800919462 >10 0.386512474268409 4.932082012588758 >50 0.015406986546410956 0.7574253925871712 >100 0.006560892349154463 0.776884258809063 >500 2.943070019940457E-4 0.1337803794646871 >1k 9.810233395277859E-5 0.15460194605643487 >5k 0.0 0.0 >10k+ 4.9051166976389294E-5 4.527239764799004 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 124805 4.447780938458435 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.5637842542033054E-5 0.0 5 0.0 0.0 0.0 3.5637842542033054E-5 0.0 6 0.0 0.0 0.0 7.127568508406611E-5 0.0 7 0.0 0.0 0.0 7.127568508406611E-5 0.0 8 0.0 0.0 0.0 7.127568508406611E-5 0.0 9 0.0 0.0 0.0 7.127568508406611E-5 0.0 10-11 0.0 3.5637842542033054E-5 0.0 1.0691352762609915E-4 0.0 12-13 0.0 3.5637842542033054E-5 0.0 1.0691352762609915E-4 0.0 14-15 0.0 3.5637842542033054E-5 0.0 1.6037029143914873E-4 0.0 16-17 0.0 3.5637842542033054E-5 0.0 2.138270552521983E-4 0.0 18-19 0.0 3.5637842542033054E-5 0.0 2.138270552521983E-4 0.0 20-21 0.0 3.5637842542033054E-5 0.0 3.207405828782975E-4 0.0 22-23 0.0 3.5637842542033054E-5 0.0 4.276541105043966E-4 1.7818921271016527E-5 24-25 0.0 7.127568508406611E-5 0.0 4.989297955884628E-4 3.5637842542033054E-5 26-27 0.0 7.127568508406611E-5 0.0 5.702054806725289E-4 3.5637842542033054E-5 28-29 0.0 7.127568508406611E-5 0.0 8.553082210087932E-4 3.5637842542033054E-5 30-31 0.0 7.127568508406611E-5 0.0 0.0017462542845596198 3.5637842542033054E-5 32-33 0.0 7.127568508406611E-5 0.0 0.005220943932407842 3.5637842542033054E-5 34-35 0.0 7.127568508406611E-5 0.0 0.010406250022273652 3.5637842542033054E-5 36-37 0.0 7.127568508406611E-5 0.0 0.019957191823538507 3.5637842542033054E-5 38-39 0.0 7.127568508406611E-5 0.0 0.03485381000610832 3.5637842542033054E-5 40-41 0.0 7.127568508406611E-5 0.0 0.05103339052019133 3.5637842542033054E-5 42-43 0.0 7.127568508406611E-5 0.0 0.0670704196641062 3.5637842542033054E-5 44-45 0.0 7.127568508406611E-5 0.0 0.08635049247934609 3.5637842542033054E-5 46-47 0.0 7.127568508406611E-5 0.0 0.1114751714714794 3.5637842542033054E-5 48-49 0.0 7.127568508406611E-5 0.0 0.1418920700811046 3.5637842542033054E-5 50-51 0.0 7.127568508406611E-5 0.0 0.17774373967838986 3.5637842542033054E-5 52-53 0.0 8.909460635508264E-5 0.0 0.219956764169428 5.345676381304958E-5 54-55 0.0 1.0691352762609915E-4 0.0 0.2635774834408765 7.127568508406611E-5 56-57 0.0 1.0691352762609915E-4 0.0 0.31849539879814937 7.127568508406611E-5 58-59 0.0 1.0691352762609915E-4 0.0 0.3800063150256984 7.127568508406611E-5 60-61 0.0 1.0691352762609915E-4 0.0 0.45113944873959644 7.127568508406611E-5 62-63 0.0 1.0691352762609915E-4 0.0 0.5212569039410464 1.0691352762609915E-4 64-65 0.0 1.0691352762609915E-4 0.0 0.5948134109478027 1.0691352762609915E-4 66-67 0.0 1.0691352762609915E-4 0.0 0.6827497874202693 1.0691352762609915E-4 68-69 0.0 1.0691352762609915E-4 0.0 0.7818764464509342 1.0691352762609915E-4 70-71 0.0 1.0691352762609915E-4 0.0 0.8939930990881702 1.0691352762609915E-4 72-73 0.0 1.0691352762609915E-4 0.0 1.0110634118387487 1.0691352762609915E-4 74-75 0.0 1.0691352762609915E-4 0.0 1.1282406381169534 1.0691352762609915E-4 76-77 0.0 1.0691352762609915E-4 0.0 1.2555390116770955 1.0691352762609915E-4 78-79 0.0 1.0691352762609915E-4 0.0 1.4055208720152415 1.0691352762609915E-4 80-81 0.0 1.0691352762609915E-4 0.0 1.5677621501878471 1.0691352762609915E-4 82-83 0.0 1.0691352762609915E-4 0.0 1.7342265127016834 1.0691352762609915E-4 84-85 0.0 1.0691352762609915E-4 0.0 1.8950244582513367 1.0691352762609915E-4 86-87 0.0 1.0691352762609915E-4 0.0 2.067208694493169 1.0691352762609915E-4 88-89 0.0 1.0691352762609915E-4 3.5637842542033054E-5 2.2612745660558105 1.0691352762609915E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2255 0.0 61.610035 1 GTATCAA 4690 0.0 49.079735 1 ATCAACG 5900 0.0 38.34953 3 TCAACGC 5995 0.0 37.57169 4 TATCAAC 6105 0.0 36.941345 2 CAACGCA 6090 0.0 36.311974 5 AACGCAG 6785 0.0 32.206036 6 ACGCAGA 7550 0.0 28.64975 7 CGCAGAG 7530 0.0 27.736906 8 GTACATG 5125 0.0 26.80832 1 TACATGG 5335 0.0 25.267778 2 GCAGAGT 8220 0.0 24.676195 9 ACATGGG 5535 0.0 24.12648 3 GAGTACT 5025 0.0 23.464964 12-13 GTACTAG 535 0.0 22.997793 1 CAGAGTA 7830 0.0 21.436762 10-11 GTACTTT 5630 0.0 21.340609 14-15 AGAGTAC 7270 0.0 21.324127 10-11 GTGTAGC 975 0.0 18.928953 1 AGTACTT 5680 0.0 18.856497 12-13 >>END_MODULE