FastQCFastQC Report
Sat 18 Jun 2016
SRR1930998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2806006
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81150.28920109222859824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72930.25990678565904707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41250.14700610048588636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44150.057.695631
GTATCAA77900.039.2877581
CAACGCA98200.031.1589265
ATCAACG98300.031.0338823
TCAACGC99100.030.8302024
TATCAAC100400.030.3396032
AACGCAG103800.029.523086
GTGGTAT20500.028.0504841
ACGCAGA118150.025.4956747
CGCAGAG118950.025.3242028
GTACATG78000.024.0664121
CGCGAAA500.001619482623.83730572-73
ACATGGG76350.022.8408153
TACATGG80450.022.5643542
TGGTATC24600.022.4082782
GCAGAGT136500.021.6508129
GAGTACT93050.021.18231612-13
CAGAGTA133100.019.8872610-11
GTACTTT100800.019.64783514-15
AGAGTAC124350.019.55090510-11
Produced by FastQC (version 0.11.5)