##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930998_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2806006 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.330820390262886 31.0 31.0 34.0 30.0 34.0 2 31.480752357621473 31.0 31.0 34.0 30.0 34.0 3 31.542961775562848 31.0 31.0 34.0 30.0 34.0 4 35.32232468497929 37.0 35.0 37.0 33.0 37.0 5 35.19789907790646 37.0 35.0 37.0 33.0 37.0 6 35.227372642824 37.0 35.0 37.0 32.0 37.0 7 35.107282022917985 37.0 35.0 37.0 32.0 37.0 8 35.111677950795546 37.0 35.0 37.0 32.0 37.0 9 36.60344347089778 39.0 35.0 39.0 32.0 39.0 10-11 36.545799082396826 38.0 35.0 39.0 32.0 39.0 12-13 36.42891907572543 38.0 35.0 39.0 32.0 39.0 14-15 37.47955884627474 39.0 36.0 41.0 32.0 41.0 16-17 37.43502847107241 39.0 36.0 41.0 32.0 41.0 18-19 37.472776786649774 39.0 36.0 41.0 32.0 41.0 20-21 37.46326789750272 39.0 36.0 41.0 32.0 41.0 22-23 37.36259651618707 39.0 36.0 41.0 32.0 41.0 24-25 37.23750252137736 39.0 36.0 40.5 31.0 41.0 26-27 36.97893696592239 39.0 36.0 40.0 31.0 41.0 28-29 36.79509434406056 39.0 36.0 40.0 30.0 41.0 30-31 36.56775537899777 38.0 35.0 40.0 30.0 41.0 32-33 36.3969348248008 38.0 35.0 40.0 30.0 41.0 34-35 36.47145480088068 38.0 35.0 40.0 30.0 41.0 36-37 36.527059101085314 38.0 35.0 40.0 30.0 41.0 38-39 36.51306804048174 39.0 35.0 40.0 30.0 41.0 40-41 36.39216131398151 39.0 35.0 40.0 30.0 41.0 42-43 36.246588923901086 38.0 35.0 40.0 30.0 41.0 44-45 36.067567745756776 38.0 35.0 40.0 29.5 41.0 46-47 35.80421549348077 38.0 35.0 40.0 28.5 41.0 48-49 35.56786835095862 38.0 34.5 40.0 27.5 41.0 50-51 35.29828179269752 38.0 34.0 40.0 27.0 41.0 52-53 35.0833608338685 38.0 34.0 40.0 26.0 41.0 54-55 34.923328923744286 38.0 34.0 40.0 26.0 41.0 56-57 34.745437643397764 38.0 33.5 40.0 26.0 41.0 58-59 34.53950330113335 38.0 33.0 40.0 26.0 41.0 60-61 34.229914155564884 37.0 33.0 40.0 25.0 41.0 62-63 33.97602018669953 37.0 33.0 40.0 25.0 41.0 64-65 33.65971954443433 36.5 32.0 39.5 23.5 41.0 66-67 33.33765323381347 36.0 32.0 39.0 23.0 41.0 68-69 32.98394390461033 36.0 31.5 39.0 22.0 40.0 70-71 32.61631069213679 35.0 31.0 38.5 22.0 40.0 72-73 32.19187058046205 35.0 31.0 38.0 21.5 40.0 74-75 31.669791333304346 35.0 31.0 37.0 20.0 39.0 76-77 30.283166358161743 33.5 28.5 36.0 19.0 38.5 78-79 31.025808391001302 34.0 30.0 36.0 20.0 39.0 80-81 30.998221493467938 34.0 30.0 36.0 20.0 38.5 82-83 30.72999612260273 34.0 30.0 35.5 19.5 37.0 84-85 30.393240427853684 34.0 30.0 35.0 18.0 37.0 86-87 30.055544785007587 34.0 30.0 35.0 17.5 36.0 88-89 29.71163835715248 34.0 29.5 35.0 12.0 36.0 90-91 29.37542952509724 34.0 29.0 35.0 7.0 36.0 92-93 29.05450202173481 34.0 29.0 35.0 3.5 35.0 94-95 28.678332476837184 34.0 29.0 35.0 2.0 35.0 96-97 28.25091785263467 34.0 29.0 35.0 2.0 35.0 98-99 27.757414809519297 33.0 28.5 35.0 2.0 35.0 100-101 26.624567267496936 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27809246184036596 1101 2 0.37783102344858577 1101 3 0.3601215136587257 1101 4 0.15804113066950265 1101 5 0.24503180166718153 1101 6 0.30192436788702537 1101 7 0.38277683457526024 1101 8 0.3757349297375612 1101 9 0.551064396130549 1101 10-11 0.5343441560177169 1101 12-13 0.41717879829958804 1101 14-15 0.4930340303426206 1101 16-17 0.5696409232016322 1101 18-19 0.49527664359543877 1101 20-21 0.47172125115364594 1101 22-23 0.43565971701082873 1101 24-25 0.4259115562141531 1101 26-27 0.446372176016709 1101 28-29 0.4351980149344996 1101 30-31 0.4303292775994052 1101 32-33 0.3930734708022712 1101 34-35 0.3941987678156025 1101 36-37 0.38422160978087305 1101 38-39 0.49266867080954313 1101 40-41 0.5463471814962872 1101 42-43 0.426841715035728 1101 44-45 0.5727698528424483 1101 46-47 0.5509875979642018 1101 48-49 0.4336603562006829 1101 50-51 0.4196733272632329 1101 52-53 0.24393058423973457 1101 54-55 0.32596696011925275 1101 56-57 0.4265622672446838 1101 58-59 0.3237699240889711 1101 60-61 0.35007297717670127 1101 62-63 0.107683953932451 1101 64-65 0.2871622349104328 1101 66-67 0.2698964980671903 1101 68-69 0.28251554981861915 1101 70-71 0.21028859998957472 1101 72-73 0.14229135489447486 1101 74-75 0.2367164367568968 1101 76-77 0.17385385895496341 1101 78-79 0.29769685655098144 1101 80-81 0.2354616917047032 1101 82-83 0.2026590328803799 1101 84-85 0.23723417368216815 1101 86-87 0.27816005242374686 1101 88-89 0.2617092823114291 1101 90-91 0.19782770814267892 1101 92-93 -0.04218316082452134 1101 94-95 0.1832204273757263 1101 96-97 0.32576618530954704 1101 98-99 0.5218669997642991 1101 100-101 0.8604908258065471 1102 1 0.3711833332634136 1102 2 0.460696980880666 1102 3 0.46109448620406823 1102 4 0.19206463766246884 1102 5 0.2984485036867994 1102 6 0.37147734341883165 1102 7 0.4911294266140729 1102 8 0.39568487193671587 1102 9 0.49022827164465355 1102 10-11 0.532336701736746 1102 12-13 0.6040877488756706 1102 14-15 0.6910814473027429 1102 16-17 0.6356804829065936 1102 18-19 0.6997544656756531 1102 20-21 0.6004617417042724 1102 22-23 0.6277222872840866 1102 24-25 0.726464545590467 1102 26-27 0.7551435521908942 1102 28-29 0.6991708361790998 1102 30-31 0.6904531390118862 1102 32-33 0.7523310860256984 1102 34-35 0.7911490566220181 1102 36-37 0.793456987256441 1102 38-39 0.7363930275588899 1102 40-41 0.8566152483375973 1102 42-43 0.841142391636815 1102 44-45 0.9005504860508751 1102 46-47 0.8575903919845587 1102 48-49 0.8652004172747638 1102 50-51 0.5888040698927526 1102 52-53 0.6378276636409836 1102 54-55 0.7972626507181388 1102 56-57 0.6984068680332243 1102 58-59 0.7577112045679186 1102 60-61 0.6418892140207078 1102 62-63 0.3957518508818154 1102 64-65 0.37296557756904036 1102 66-67 0.3287756018525769 1102 68-69 0.37242496468031305 1102 70-71 0.41246823303088576 1102 72-73 0.4633262494932353 1102 74-75 0.5509497202499851 1102 76-77 0.5197172793801705 1102 78-79 0.6988021678003378 1102 80-81 0.5941662063012707 1102 82-83 0.538883026211284 1102 84-85 0.7372070409103308 1102 86-87 0.7658308343716875 1102 88-89 0.7178448611257657 1102 90-91 0.7434887020204357 1102 92-93 0.7428171259546623 1102 94-95 0.8814125936562291 1102 96-97 0.992157506877021 1102 98-99 0.8730002349004806 1102 100-101 1.0463340579661633 1103 1 0.34411120216706337 1103 2 0.3816582003726481 1103 3 0.3815324108481697 1103 4 0.20954030751470043 1103 5 0.23047650242330064 1103 6 0.3661957526601398 1103 7 0.35020553556798006 1103 8 0.3778869619933971 1103 9 0.5304640846132855 1103 10-11 0.4221042877224832 1103 12-13 0.42685431757718106 1103 14-15 0.46449261629081917 1103 16-17 0.43340978921963824 1103 18-19 0.47297370784094994 1103 20-21 0.5276482121844168 1103 22-23 0.5243393668496452 1103 24-25 0.5108892275240038 1103 26-27 0.5316035712394509 1103 28-29 0.4809057801924368 1103 30-31 0.5269007392660185 1103 32-33 0.7058963249313521 1103 34-35 0.6177226343758022 1103 36-37 0.6001365931698999 1103 38-39 0.6901822011085414 1103 40-41 0.605402254002918 1103 42-43 0.6492274591775313 1103 44-45 0.678896564462228 1103 46-47 0.7030090098472144 1103 48-49 0.6064497912306095 1103 50-51 0.3973160485045639 1103 52-53 0.4953219376639595 1103 54-55 0.6417741200873124 1103 56-57 0.5181635004981615 1103 58-59 0.5194454039340073 1103 60-61 0.4753573845545347 1103 62-63 0.3944438539708983 1103 64-65 0.43053263485522564 1103 66-67 0.48351083771959935 1103 68-69 0.5573431555718287 1103 70-71 0.47936866288451796 1103 72-73 0.3570158444892044 1103 74-75 0.2767960658936168 1103 76-77 0.41692617335080584 1103 78-79 0.5056146692265777 1103 80-81 0.5510353369496279 1103 82-83 0.4853306384758618 1103 84-85 0.6175066773543278 1103 86-87 0.6365134717200718 1103 88-89 0.5982399805635836 1103 90-91 0.5247085182847329 1103 92-93 0.3885370256915124 1103 94-95 0.6086639205095317 1103 96-97 0.5916576323861165 1103 98-99 0.49558088199894 1103 100-101 0.8634816626641673 1104 1 0.2750310906597271 1104 2 0.3580742341963834 1104 3 0.3531950388423404 1104 4 0.27056694158981287 1104 5 0.153501050732757 1104 6 0.24362762725083797 1104 7 0.32758720599113644 1104 8 0.2544824139896278 1104 9 0.33734017437880226 1104 10-11 0.37707397122459696 1104 12-13 0.35607226127562086 1104 14-15 0.49222212987475444 1104 16-17 0.5149283065624175 1104 18-19 0.5221739095982088 1104 20-21 0.458270167748438 1104 22-23 0.4221628927341854 1104 24-25 0.44761886691228625 1104 26-27 0.45171568996114075 1104 28-29 0.658047540536316 1104 30-31 0.5815763697868661 1104 32-33 0.5624764217419269 1104 34-35 0.46998975817081856 1104 36-37 0.5506530376801848 1104 38-39 0.6757007678324669 1104 40-41 0.5427124158202403 1104 42-43 0.5938203997896423 1104 44-45 0.7102326067042739 1104 46-47 0.6273683188403325 1104 48-49 0.5216380215866039 1104 50-51 0.6010620201198122 1104 52-53 0.47551498268192915 1104 54-55 0.6078891875690076 1104 56-57 0.5677953757942973 1104 58-59 0.5613708807266207 1104 60-61 0.49014786151764156 1104 62-63 0.4711523535247082 1104 64-65 0.42124833229927816 1104 66-67 0.3365703525850847 1104 68-69 0.32799341123684655 1104 70-71 0.5184530188191303 1104 72-73 0.38448763550818654 1104 74-75 0.5276986239546808 1104 76-77 0.5847261373731136 1104 78-79 0.6259235483097818 1104 80-81 0.562407639063494 1104 82-83 0.47394487412035247 1104 84-85 0.559349558297555 1104 86-87 0.5360356503222796 1104 88-89 0.6469307334732477 1104 90-91 0.6346415950712441 1104 92-93 0.5892924396877781 1104 94-95 0.5437712128118335 1104 96-97 0.6665046390067175 1104 98-99 0.7713654837811319 1104 100-101 0.9054916752798725 1105 1 0.285817846938631 1105 2 0.34524977857920547 1105 3 0.36194294395473037 1105 4 0.173602239366204 1105 5 0.22148817329303938 1105 6 0.21949233350584052 1105 7 0.29438241668931653 1105 8 0.2838174059556309 1105 9 0.40463216859662765 1105 10-11 0.41576624303302623 1105 12-13 0.4266068247615138 1105 14-15 0.3869165210471479 1105 16-17 0.4821852276103442 1105 18-19 0.5093457681306646 1105 20-21 0.4281549273330896 1105 22-23 0.46406548261997926 1105 24-25 0.40705308888110636 1105 26-27 0.42783253141773514 1105 28-29 0.5248117094634424 1105 30-31 0.3714330247624744 1105 32-33 0.5334589312301077 1105 34-35 0.48830676433885145 1105 36-37 0.4291624567556127 1105 38-39 0.5324231253945086 1105 40-41 0.4988607021877769 1105 42-43 0.5011892209510265 1105 44-45 0.5065576987065583 1105 46-47 0.49911305066592604 1105 48-49 0.4155219864097788 1105 50-51 0.33233980077716296 1105 52-53 0.2560925669525673 1105 54-55 0.37869512178809117 1105 56-57 0.4209744793263823 1105 58-59 0.39471371664293287 1105 60-61 0.5654066485273219 1105 62-63 0.44739675995882067 1105 64-65 0.37431950952533555 1105 66-67 0.463493243443601 1105 68-69 0.18881051818986094 1105 70-71 0.2518887496829407 1105 72-73 0.3875688912313322 1105 74-75 0.46965103041182843 1105 76-77 0.5159291643777273 1105 78-79 0.47985606269324066 1105 80-81 0.28621039999578457 1105 82-83 0.40112853889225875 1105 84-85 0.4735931238970501 1105 86-87 0.5092517275408461 1105 88-89 0.5749480915248171 1105 90-91 0.5497803615783816 1105 92-93 0.4873370960686181 1105 94-95 0.5231522203159926 1105 96-97 0.44829008796056513 1105 98-99 0.47513985214731846 1105 100-101 0.6589669714437107 1106 1 0.2406044591393126 1106 2 0.29022371164020555 1106 3 0.2563330227197689 1106 4 0.12534161511379693 1106 5 0.21833283239164558 1106 6 0.2603127085989385 1106 7 0.2963811077001779 1106 8 0.31225451805475046 1106 9 0.33982231055423995 1106 10-11 0.25243892804058277 1106 12-13 0.3211994583122575 1106 14-15 0.30964440928655534 1106 16-17 0.3503326694393252 1106 18-19 0.32722317452665806 1106 20-21 0.2704378721072871 1106 22-23 0.2236119630190032 1106 24-25 0.25148458135442553 1106 26-27 0.33578576890899114 1106 28-29 0.30234144382892936 1106 30-31 0.2714757925974496 1106 32-33 0.2708558483349819 1106 34-35 0.38332651201991297 1106 36-37 0.3156459025566889 1106 38-39 0.3622229403305255 1106 40-41 0.3543152373837799 1106 42-43 0.4645888688780886 1106 44-45 0.41444605096005205 1106 46-47 0.293193147260304 1106 48-49 0.2064829649920057 1106 50-51 0.15539132529814026 1106 52-53 0.19835768239186535 1106 54-55 0.0958827134737561 1106 56-57 0.27685375893097586 1106 58-59 0.13708152002373453 1106 60-61 0.1739180119072259 1106 62-63 0.1810953313551522 1106 64-65 -0.007791122626144897 1106 66-67 -0.15440481338458056 1106 68-69 0.02299713941805237 1106 70-71 0.06088500326573154 1106 72-73 0.2133573315030617 1106 74-75 0.2616169966940767 1106 76-77 0.26170372485076854 1106 78-79 0.09784935976606945 1106 80-81 -0.018303468518514165 1106 82-83 0.1424202629212381 1106 84-85 0.17170592265125606 1106 86-87 0.1998283598277375 1106 88-89 0.3112223247505739 1106 90-91 0.348652486880237 1106 92-93 0.27431412105225306 1106 94-95 0.4881602322323104 1106 96-97 0.4816459524576189 1106 98-99 0.6427606515334503 1106 100-101 0.8721018876006994 1107 1 0.1270487350924121 1107 2 0.1727981194720165 1107 3 0.2182159492179494 1107 4 0.14261882475424414 1107 5 0.16437191642982896 1107 6 0.2279255315642459 1107 7 0.31267817996956637 1107 8 0.2465294977152226 1107 9 0.2963774424999315 1107 10-11 0.20557094696281553 1107 12-13 0.313243935561907 1107 14-15 0.268359415703209 1107 16-17 0.2033161942008519 1107 18-19 0.3443987981753409 1107 20-21 0.3003819618566155 1107 22-23 0.24241262763697335 1107 24-25 0.20448900380568347 1107 26-27 0.23844763446540185 1107 28-29 0.3545703744476967 1107 30-31 0.4011130256718829 1107 32-33 0.35547430028056937 1107 34-35 0.2622371261708665 1107 36-37 0.33361311551040274 1107 38-39 0.369358295738607 1107 40-41 0.282501643883144 1107 42-43 0.34730717159273183 1107 44-45 0.41219206990550816 1107 46-47 0.32978430985950524 1107 48-49 0.3356818154404593 1107 50-51 0.3442836673665326 1107 52-53 0.17013274019541313 1107 54-55 0.3256348391885453 1107 56-57 0.3951526557294329 1107 58-59 0.45320250246521 1107 60-61 0.39045558622128596 1107 62-63 0.23736481688796118 1107 64-65 0.1536589359141729 1107 66-67 0.1705733512088372 1107 68-69 0.2523255064243486 1107 70-71 0.3329006970735051 1107 72-73 0.2990463435156627 1107 74-75 0.38235630158689915 1107 76-77 0.31924794713074434 1107 78-79 0.4605951867028324 1107 80-81 0.32110303262773243 1107 82-83 0.46227871679584 1107 84-85 0.48620699923950284 1107 86-87 0.4815903922977469 1107 88-89 0.5323813918919882 1107 90-91 0.6258850120802641 1107 92-93 0.5077094999836333 1107 94-95 0.661012034568742 1107 96-97 0.7274616142937091 1107 98-99 0.8190679404511556 1107 100-101 0.9368145086443143 1108 1 0.10062237351270298 1108 2 0.1487824103728883 1108 3 0.1430743608041496 1108 4 0.1606561695182478 1108 5 0.09441792299412555 1108 6 0.17076343494575497 1108 7 0.1623391683000861 1108 8 0.17227554874558137 1108 9 0.1776967861781884 1108 10-11 0.1719264184746052 1108 12-13 0.21625730161579781 1108 14-15 0.2522988261545578 1108 16-17 0.2786518591433662 1108 18-19 0.31390792919011545 1108 20-21 0.291779359952983 1108 22-23 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-0.9304350632805445 2316 86-87 -0.8492360518745308 2316 88-89 -1.0359844189148788 2316 90-91 -0.9870671574587071 2316 92-93 -0.882325456129859 2316 94-95 -1.3462953969770552 2316 96-97 -1.2168933743337078 2316 98-99 -1.4001067088941923 2316 100-101 -1.7795051484285587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 25.0 5 67.0 6 113.0 7 366.0 8 941.0 9 1776.0 10 3157.0 11 5201.0 12 7295.0 13 9868.0 14 12254.0 15 15820.0 16 19031.0 17 22023.0 18 19179.0 19 14803.0 20 13865.0 21 14497.0 22 16029.0 23 18809.0 24 23133.0 25 29655.0 26 37813.0 27 49102.0 28 62615.0 29 78890.0 30 96299.0 31 116232.0 32 139896.0 33 170280.0 34 212410.0 35 262798.0 36 345595.0 37 469161.0 38 438190.0 39 78796.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.450603089792736 21.67751767357453 14.881370719530814 22.99050851710192 2 15.005643604336827 21.621615842151577 40.990779558711836 22.38196099479976 3 18.560120486522067 26.48076930615829 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27.77610675417445 30.459055922349197 20.938895893724798 24-25 20.74330641650655 28.01870783907067 30.595695386600696 20.64229035782208 26-27 20.688599525219296 28.172953180891735 30.461911518011696 20.676535775877277 28-29 20.570206699928725 28.140573770491805 30.582483962936564 20.70673556664291 30-31 20.926941213746193 27.93426067134292 30.46602601959007 20.672772095320816 32-33 20.83623918182238 28.178653674130178 30.26132942278754 20.723777721259903 34-35 20.90388351694648 28.106373370373184 30.477429822282147 20.51231329039819 36-37 20.944740627727995 28.035284686097693 30.424007471195857 20.595967214978458 38-39 20.81144718868396 28.250134800475696 30.428146980807906 20.510271030032442 40-41 20.876205763430253 28.026100246600926 30.47573362395381 20.62196036601501 42-43 20.825764605602533 28.11252251460743 30.164646448265543 20.897066431524497 44-45 20.769428436911152 28.397831347029022 30.08109638722972 20.751643828830098 46-47 20.97510225183025 28.2857672901425 29.7098883325008 21.02924212552645 48-49 21.07193252020263 28.48744644382737 29.462711431269 20.977909604701004 50-51 21.114958107530075 28.568500775865825 29.215579300056916 21.100961816547187 52-53 20.992014391364037 28.737380440646326 29.09982260545913 21.170782562530505 54-55 21.04098619369462 28.72319967948598 29.186023777991288 21.04979034882811 56-57 21.009010595165456 28.76119820030451 29.117084989421944 21.11270621510809 58-59 20.933281411096686 28.646442539638972 29.339123197387966 21.081152851876375 60-61 21.019450662925347 28.826726678079968 29.25397616052078 20.899846498473906 62-63 20.964916479545508 28.654738779338967 29.28759885033903 21.09274589077649 64-65 20.755351241149288 28.591829221830395 29.258196883091927 21.394622653928394 66-67 20.795223883583144 28.62513377495369 29.13309900052179 21.44654334094138 68-69 20.82566354087457 28.642269447601677 29.15801889193805 21.374048119585705 70-71 20.901810923265092 28.423379399426175 29.159861773144495 21.514947904164238 72-73 20.942763743112558 28.646559884860697 29.100517013174482 21.310159358852264 74-75 20.91344306981022 28.797713408478625 28.90545350719929 21.383390014511857 76-77 20.794593074385403 28.741970188697774 28.99978125878871 21.46365547812811 78-79 20.74610654770019 28.630076813209627 29.084986363227312 21.538830275862868 80-81 20.750657963902416 28.817856703747857 28.930062490979168 21.50142284137056 82-83 20.870625365733357 28.718737593576066 29.108865055883705 21.301771984806877 84-85 20.969217457125893 28.68653880283934 28.81836318240232 21.525880557632448 86-87 20.71217951779148 28.719325617978008 28.998138279105607 21.570356585124905 88-89 20.8834164170828 28.595682686125894 28.880589621397547 21.64031127539376 90-91 20.753021387610797 28.600449777784114 29.08396089570644 21.56256793889865 92-93 20.72967905670331 28.68054664442448 28.910275603461795 21.679498695410413 94-95 20.500630630726953 28.56883711266283 28.987540989215304 21.942991267394913 96-97 20.55580432753963 28.48674031465726 28.982338060278213 21.9751172975249 98-99 20.469361077631337 28.60610419222197 28.876898338777607 22.047636391369085 100-101 20.47028766153743 28.558420758900727 28.73201268992297 22.239278889638868 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 221.0 1 164.5 2 153.0 3 234.5 4 214.5 5 212.0 6 391.5 7 808.5 8 1373.0 9 1797.0 10 1889.5 11 1737.5 12 1787.5 13 2107.5 14 2409.0 15 2691.0 16 3076.0 17 3499.5 18 3874.5 19 4300.5 20 4886.5 21 5772.5 22 6959.5 23 8633.0 24 10875.0 25 13575.0 26 17190.5 27 21701.0 28 26865.5 29 33703.0 30 41286.0 31 49180.0 32 59102.0 33 68539.0 34 76687.0 35 84419.0 36 94579.0 37 106193.0 38 111167.5 39 111831.0 40 111848.5 41 110733.0 42 109872.5 43 111953.5 44 114484.5 45 117365.5 46 117727.0 47 115068.0 48 113839.0 49 109741.0 50 102275.5 51 95007.0 52 88062.5 53 80216.5 54 72010.5 55 61353.0 56 49640.0 57 39003.5 58 30046.5 59 22766.5 60 16295.0 61 11244.5 62 7671.5 63 5019.0 64 3069.0 65 1753.5 66 895.0 67 459.0 68 253.5 69 134.5 70 80.5 71 51.0 72 27.0 73 16.0 74 15.5 75 10.5 76 8.0 77 6.0 78 2.0 79 2.5 80 2.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0043478167901280325 2 0.006236622444855785 3 0.0017462542845596196 4 0.1691728385470309 5 0.0021739083950640162 6 3.920162679623636E-4 7 3.5637842542033054E-5 8 8.909460635508264E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.8510274033626443E-4 24-25 5.880244019435453E-4 26-27 0.0028153895608206115 28-29 2.138270552521983E-4 30-31 0.007020654980780512 32-33 0.02140052444649085 34-35 0.013506742323430528 36-37 0.001051316354989975 38-39 2.494648977942314E-4 40-41 0.0019779002610828345 42-43 0.011671393432515826 44-45 0.007448309091284908 46-47 0.011065550109301263 48-49 0.010940817660404147 50-51 0.187597603141262 52-53 0.11454002593009423 54-55 0.01826439430279194 56-57 0.007056292823322545 58-59 0.018834599783464467 60-61 0.1221665242340893 62-63 0.23421190118624122 64-65 0.2396823100164433 66-67 0.2493045275027922 68-69 0.24502798639774825 70-71 0.2334278686503165 72-73 0.16143942671540973 74-75 0.27102579253216136 76-77 0.2100316250214718 78-79 0.006183165681042734 80-81 3.5637842542033054E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.127568508406611E-5 90-91 0.006272260287397817 92-93 0.018745505177109385 94-95 0.0030292166160728095 96-97 1.7818921271016527E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2806006.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.0043372345338 #Duplication Level Percentage of deduplicated Percentage of total 1 69.3040362378883 33.9619836351583 2 16.268368011462325 15.944411845784037 3 6.05960267174634 8.90840438500619 4 2.7946374020674236 5.477974147966139 5 1.569581861635971 3.8458159432408245 6 0.9551966762719438 2.808526802960169 7 0.6130573616393883 2.102972878572309 8 0.42756034758123906 1.6761849168788423 9 0.32029521493497615 1.4126269254552954 >10 1.5161687486615136 13.262829102726464 >50 0.111979896050559 3.7907227761673727 >100 0.056666243868584494 4.987961504951411 >500 0.0019726104402987025 0.6338814083804168 >1k 7.305964593439482E-4 0.634060420338796 >5k 1.4611929186878964E-4 0.5516433064133354 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8115 0.28920109222859824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7293 0.25990678565904707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4125 0.14700610048588636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.5637842542033054E-5 0.0 6 0.0 0.0 0.0 3.5637842542033054E-5 0.0 7 0.0 0.0 0.0 3.5637842542033054E-5 0.0 8 0.0 0.0 0.0 3.5637842542033054E-5 0.0 9 0.0 0.0 0.0 3.5637842542033054E-5 0.0 10-11 0.0 0.0 0.0 3.5637842542033054E-5 0.0 12-13 0.0 0.0 0.0 3.5637842542033054E-5 1.7818921271016527E-5 14-15 0.0 0.0 0.0 7.127568508406611E-5 3.5637842542033054E-5 16-17 0.0 0.0 0.0 7.127568508406611E-5 1.0691352762609915E-4 18-19 0.0 0.0 0.0 8.909460635508264E-5 1.0691352762609915E-4 20-21 0.0 0.0 0.0 1.2473244889711567E-4 1.0691352762609915E-4 22-23 0.0 0.0 0.0 1.7818921271016528E-4 1.4255137016813221E-4 24-25 0.0 0.0 0.0 2.494648977942314E-4 1.4255137016813221E-4 26-27 0.0 0.0 0.0 3.38559504149314E-4 1.4255137016813221E-4 28-29 0.0 0.0 0.0 8.909460635508264E-4 1.4255137016813221E-4 30-31 0.0 0.0 0.0 0.0030648544586148424 1.4255137016813221E-4 32-33 0.0 0.0 0.0 0.010032052675582305 1.4255137016813221E-4 34-35 0.0 0.0 0.0 0.020224475642603757 1.4255137016813221E-4 36-37 0.0 0.0 0.0 0.039255083560049406 1.4255137016813221E-4 38-39 0.0 0.0 0.0 0.07033128225670224 1.4255137016813221E-4 40-41 0.0 0.0 0.0 0.10661060596449187 1.4255137016813221E-4 42-43 0.0 1.7818921271016527E-5 0.0 0.14203462145127274 1.4255137016813221E-4 44-45 0.0 3.5637842542033054E-5 0.0 0.1855484271950951 1.4255137016813221E-4 46-47 0.0 3.5637842542033054E-5 0.0 0.24148202106481598 1.7818921271016528E-4 48-49 0.0 3.5637842542033054E-5 0.0 0.30627161880623205 1.7818921271016528E-4 50-51 0.0 3.5637842542033054E-5 0.0 0.37799277692207356 1.7818921271016528E-4 52-53 0.0 3.5637842542033054E-5 0.0 0.4514423704012037 1.7818921271016528E-4 54-55 0.0 3.5637842542033054E-5 0.0 0.5312889566166288 1.7818921271016528E-4 56-57 0.0 3.5637842542033054E-5 0.0 0.6311461914194054 1.7818921271016528E-4 58-59 0.0 3.5637842542033054E-5 0.0 0.7436192224820617 1.7818921271016528E-4 60-61 0.0 3.5637842542033054E-5 0.0 0.8697950040021297 1.7818921271016528E-4 62-63 0.0 3.5637842542033054E-5 0.0 0.9988752696893735 1.7818921271016528E-4 64-65 0.0 3.5637842542033054E-5 0.0 1.1329448333325018 1.7818921271016528E-4 66-67 0.0 3.5637842542033054E-5 0.0 1.292727100369707 1.7818921271016528E-4 68-69 0.0 3.5637842542033054E-5 0.0 1.473874254010861 1.7818921271016528E-4 70-71 0.0 3.5637842542033054E-5 0.0 1.682034892298876 1.7818921271016528E-4 72-73 0.0 3.5637842542033054E-5 0.0 1.899924661600866 1.7818921271016528E-4 74-75 0.0 3.5637842542033054E-5 0.0 2.1207545529125738 1.7818921271016528E-4 76-77 0.0 3.5637842542033054E-5 0.0 2.363537355230174 1.7818921271016528E-4 78-79 0.0 3.5637842542033054E-5 0.0 2.648194622534663 1.7818921271016528E-4 80-81 0.0 3.5637842542033054E-5 0.0 2.96506849949715 1.7818921271016528E-4 82-83 0.0 3.5637842542033054E-5 0.0 3.288695747621352 1.7818921271016528E-4 84-85 0.0 3.5637842542033054E-5 0.0 3.6103629144057425 1.7818921271016528E-4 86-87 0.0 3.5637842542033054E-5 0.0 3.956816200678117 1.7818921271016528E-4 88-89 0.0 3.5637842542033054E-5 0.0 4.349473949806237 1.7818921271016528E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4415 0.0 57.69563 1 GTATCAA 7790 0.0 39.287758 1 CAACGCA 9820 0.0 31.158926 5 ATCAACG 9830 0.0 31.033882 3 TCAACGC 9910 0.0 30.830202 4 TATCAAC 10040 0.0 30.339603 2 AACGCAG 10380 0.0 29.52308 6 GTGGTAT 2050 0.0 28.050484 1 ACGCAGA 11815 0.0 25.495674 7 CGCAGAG 11895 0.0 25.324202 8 GTACATG 7800 0.0 24.066412 1 CGCGAAA 50 0.0016194826 23.837305 72-73 ACATGGG 7635 0.0 22.840815 3 TACATGG 8045 0.0 22.564354 2 TGGTATC 2460 0.0 22.408278 2 GCAGAGT 13650 0.0 21.650812 9 GAGTACT 9305 0.0 21.182316 12-13 CAGAGTA 13310 0.0 19.88726 10-11 GTACTTT 10080 0.0 19.647835 14-15 AGAGTAC 12435 0.0 19.550905 10-11 >>END_MODULE