FastQCFastQC Report
Sat 18 Jun 2016
SRR1930997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668996
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN765024.583713801590897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.054.192381
GTATCAA25550.043.237641
ATCAACG28450.036.5693
TCAACGC29050.036.3302764
CAACGCA29950.035.3926055
AACGCAG32150.033.0440566
TATCAAC31850.032.901372
GTGGTAT6300.029.3613741
ACGCAGA37000.028.599597
CGCAGAG37900.027.9066728
GTACATG28850.024.7561681
TAACGCA1451.4538149E-524.3780334
TACATGG28850.023.8590032
ACATGGG29350.023.402323
GCAGAGT41600.023.267719
GAGTACT26400.021.56300412-13
TGGTATC7600.020.9527662
GTACTAG3051.717126E-920.2160281
GCCGATA1300.004811921619.7569522
AGAGTAC37450.019.54537810-11
Produced by FastQC (version 0.11.5)