##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930997_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1668996 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.16112501168367 30.0 2.0 31.0 2.0 33.0 2 19.802275739426577 27.0 2.0 31.0 2.0 33.0 3 20.505402649257398 27.0 2.0 31.0 2.0 33.0 4 21.95832045133721 30.0 2.0 35.0 2.0 37.0 5 21.27885267550072 30.0 2.0 35.0 2.0 37.0 6 22.327607136266355 30.0 2.0 35.0 2.0 37.0 7 22.556950406112417 30.0 2.0 35.0 2.0 37.0 8 23.034393731321106 32.0 2.0 35.0 2.0 37.0 9 24.400565369839114 32.0 2.0 37.0 2.0 39.0 10-11 24.80440156836805 32.5 2.0 37.0 2.0 39.0 12-13 24.732938545089382 33.0 2.0 37.0 2.0 39.0 14-15 24.710167070502266 32.5 2.0 38.0 2.0 40.0 16-17 23.4867830719786 31.5 2.0 38.0 2.0 40.0 18-19 22.37624745655472 30.0 2.0 38.0 2.0 40.0 20-21 22.02120466436109 28.0 2.0 38.0 2.0 40.0 22-23 21.63684784145678 27.0 2.0 37.0 2.0 40.0 24-25 21.475917857202774 27.0 2.0 37.0 2.0 40.0 26-27 21.20052983949632 27.0 2.0 37.0 2.0 39.5 28-29 21.014594103281254 27.0 2.0 37.0 2.0 39.0 30-31 21.03406479104803 27.0 2.0 37.0 2.0 39.0 32-33 20.92215559533995 26.5 2.0 37.0 2.0 39.0 34-35 20.604215348628756 25.0 2.0 36.5 2.0 39.0 36-37 20.125214200633195 25.0 2.0 36.0 2.0 39.0 38-39 20.034645679198753 24.0 2.0 36.0 2.0 39.0 40-41 19.92578951657164 24.0 2.0 36.0 2.0 39.0 42-43 19.79518614783978 24.0 2.0 35.5 2.0 39.0 44-45 19.418604058967187 24.0 2.0 35.0 2.0 39.0 46-47 19.081708703915407 22.5 2.0 35.0 2.0 39.0 48-49 18.863147065661032 20.5 2.0 35.0 2.0 39.0 50-51 16.587190742218674 11.5 2.0 33.0 2.0 37.5 52-53 18.502704620023056 20.5 2.0 34.5 2.0 38.0 54-55 19.288814952222772 23.0 2.0 36.0 2.0 39.0 56-57 19.27043234375637 23.0 2.0 36.0 2.0 39.0 58-59 18.993992795668774 22.0 2.0 36.0 2.0 39.0 60-61 18.707813559768866 20.5 2.0 35.5 2.0 39.0 62-63 18.26335503500308 18.0 2.0 35.0 2.0 39.0 64-65 17.921672370694715 14.5 2.0 34.0 2.0 38.5 66-67 17.57281383538367 12.5 2.0 34.0 2.0 38.0 68-69 17.14106984079051 8.0 2.0 34.0 2.0 38.0 70-71 16.793558223027496 8.0 2.0 33.5 2.0 37.5 72-73 16.398879326253628 6.5 2.0 33.0 2.0 37.0 74-75 15.953222476267168 2.0 2.0 32.0 2.0 36.0 76-77 15.547666381465264 2.0 2.0 32.0 2.0 36.0 78-79 15.18096328571189 2.0 2.0 31.0 2.0 35.5 80-81 14.744392137548562 2.0 2.0 31.0 2.0 35.0 82-83 14.283474915458156 2.0 2.0 31.0 2.0 34.5 84-85 13.88799973157515 2.0 2.0 30.0 2.0 34.0 86-87 13.40312049879089 2.0 2.0 29.5 2.0 34.0 88-89 12.984247116230357 2.0 2.0 29.0 2.0 34.0 90-91 12.505571912694819 2.0 2.0 29.0 2.0 34.0 92-93 11.961680555255974 2.0 2.0 29.0 2.0 34.0 94-95 11.389207643397587 2.0 2.0 26.5 2.0 34.0 96-97 10.744720778240332 2.0 2.0 25.5 2.0 34.0 98-99 10.060348257275631 2.0 2.0 24.5 2.0 33.0 100-101 9.115472415751746 2.0 2.0 17.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.528688115238687 1101 2 9.13119195534701 1101 3 8.486076142617168 1101 4 10.440266184533733 1101 5 11.273368342969714 1101 6 10.370765207533882 1101 7 10.101457904911474 1101 8 9.529137922352081 1101 9 9.491660968908342 1101 10-11 8.802852368541139 1101 12-13 8.715717620956127 1101 14-15 9.352368569492146 1101 16-17 10.515941832630574 1101 18-19 11.305812770791174 1101 20-21 11.409350361379925 1101 22-23 11.582503529416304 1101 24-25 11.326101205208733 1101 26-27 11.369576618049802 1101 28-29 11.258794940321842 1101 30-31 11.022262307694305 1101 32-33 11.20422976054899 1101 34-35 10.873643540386954 1101 36-37 11.149781884564671 1101 38-39 10.845096544538428 1101 40-41 10.655766939170764 1101 42-43 10.517176349392795 1101 44-45 10.334181733532962 1101 46-47 10.236788760784698 1101 48-49 10.49307246290699 1101 50-51 12.242482672493512 1101 52-53 11.245060675030018 1101 54-55 10.918904466633837 1101 56-57 10.914641544166631 1101 58-59 10.884441737408277 1101 60-61 10.225207870991497 1101 62-63 10.405964052556921 1101 64-65 10.372563610108788 1101 66-67 9.753476041857631 1101 68-69 10.019527274368993 1101 70-71 9.850395994045524 1101 72-73 9.65497735568345 1101 74-75 9.247819463542065 1101 76-77 9.172390808953201 1101 78-79 9.064639038177084 1101 80-81 8.891109196046864 1101 82-83 8.489651537670419 1101 84-85 8.350569025138443 1101 86-87 8.098941480113774 1101 88-89 7.8730925910242355 1101 90-91 7.455883973588017 1101 92-93 7.093721729690321 1101 94-95 6.925206210166012 1101 96-97 6.5002358582776925 1101 98-99 6.360303017879675 1101 100-101 5.517119128230872 1102 1 7.768331421439466 1102 2 9.119900512027574 1102 3 8.620514631135467 1102 4 10.42269620240151 1102 5 11.232148370764033 1102 6 10.19766283178112 1102 7 9.951294116942446 1102 8 9.631646029029337 1102 9 9.123659182130496 1102 10-11 8.358308658660917 1102 12-13 8.468889978841112 1102 14-15 9.0435000300176 1102 16-17 9.867664087941272 1102 18-19 10.839534066536 1102 20-21 11.042783787360992 1102 22-23 11.164492643306847 1102 24-25 11.001325379651195 1102 26-27 11.240850028148412 1102 28-29 10.963215561061709 1102 30-31 11.097364881977942 1102 32-33 11.044999729445824 1102 34-35 10.716473267076122 1102 36-37 10.93699401480092 1102 38-39 10.674020838099409 1102 40-41 10.438541077215898 1102 42-43 10.017966336819175 1102 44-45 9.979618005123196 1102 46-47 10.13638177249802 1102 48-49 9.831050591422642 1102 50-51 11.83815800834157 1102 52-53 10.635070436131205 1102 54-55 10.202642306617957 1102 56-57 10.267207555681424 1102 58-59 10.466244728606743 1102 60-61 10.424739669019424 1102 62-63 10.332209833775895 1102 64-65 10.040640673175428 1102 66-67 10.0746162708299 1102 68-69 9.561380063727743 1102 70-71 9.235057829794314 1102 72-73 9.062805262506295 1102 74-75 8.927738065884729 1102 76-77 8.754193800151663 1102 78-79 8.454892826807885 1102 80-81 8.136679475975393 1102 82-83 8.191578114336423 1102 84-85 8.018071175889547 1102 86-87 7.782156522136143 1102 88-89 7.4693387031280025 1102 90-91 7.171050077750552 1102 92-93 7.038778310988716 1102 94-95 6.650125143393465 1102 96-97 6.200863712158972 1102 98-99 6.006847753502731 1102 100-101 5.312578561756119 1103 1 7.89256155545031 1103 2 9.32228734278226 1103 3 8.435939524008386 1103 4 10.571654192829712 1103 5 11.30360030496001 1103 6 10.671846119675127 1103 7 10.062803701404729 1103 8 9.674876694806908 1103 9 9.484054588678823 1103 10-11 9.019857422232924 1103 12-13 8.741935489913956 1103 14-15 9.636660672808215 1103 16-17 10.48519614165815 1103 18-19 11.188811081833432 1103 20-21 11.635612075944326 1103 22-23 11.807557061641266 1103 24-25 11.803551590123906 1103 26-27 11.666961005614443 1103 28-29 11.202217977927432 1103 30-31 11.076931604634861 1103 32-33 11.276246863110593 1103 34-35 11.319046567884467 1103 36-37 11.70754985874849 1103 38-39 11.068994446713468 1103 40-41 10.728494625000344 1103 42-43 10.419946288650674 1103 44-45 10.518311948046904 1103 46-47 10.249814790991685 1103 48-49 10.374810386200071 1103 50-51 12.280069526859148 1103 52-53 11.30580782636639 1103 54-55 10.884692752711157 1103 56-57 10.408114133551486 1103 58-59 10.360659138078379 1103 60-61 10.406384924337797 1103 62-63 10.377291835582216 1103 64-65 9.86131412479169 1103 66-67 9.95008508369584 1103 68-69 9.981779120896885 1103 70-71 9.719467428043142 1103 72-73 9.338277486838702 1103 74-75 9.264787435783354 1103 76-77 9.02054105508176 1103 78-79 8.455123188718758 1103 80-81 8.278950816280782 1103 82-83 8.185687707520803 1103 84-85 7.928762149078937 1103 86-87 7.876932511883455 1103 88-89 7.270371076186279 1103 90-91 7.169794165714334 1103 92-93 6.832307216308914 1103 94-95 6.430341592148315 1103 96-97 6.161953475227348 1103 98-99 5.809829058666498 1103 100-101 5.049679173991256 1104 1 7.562896074471208 1104 2 9.381955232330974 1104 3 8.290048244391144 1104 4 10.578755731048403 1104 5 11.065439377130009 1104 6 10.225237440115581 1104 7 10.021616140510101 1104 8 9.189518280598254 1104 9 9.06395276197609 1104 10-11 8.60276664773491 1104 12-13 8.427512546451382 1104 14-15 9.057926471391927 1104 16-17 9.97777346082492 1104 18-19 10.738517937139637 1104 20-21 11.072320956081935 1104 22-23 11.159325931491413 1104 24-25 11.198354590902273 1104 26-27 11.211844768317267 1104 28-29 10.798357666800115 1104 30-31 10.701071595599323 1104 32-33 10.831759289696848 1104 34-35 10.695642755540614 1104 36-37 10.753218787928379 1104 38-39 10.429435868123338 1104 40-41 10.132007768714388 1104 42-43 9.885810257537944 1104 44-45 10.234582958394224 1104 46-47 9.883694010070819 1104 48-49 9.86830537719175 1104 50-51 11.587719218779963 1104 52-53 10.507472971234368 1104 54-55 10.269303047509194 1104 56-57 9.989648467234993 1104 58-59 9.838173201675506 1104 60-61 9.770133366251805 1104 62-63 9.643342672730252 1104 64-65 9.233162062402933 1104 66-67 9.312733680193755 1104 68-69 9.021389544968429 1104 70-71 8.699479191932557 1104 72-73 8.549075805194452 1104 74-75 8.487173593448048 1104 76-77 8.148458213894946 1104 78-79 8.02719010885222 1104 80-81 7.765755562931917 1104 82-83 7.345239114833056 1104 84-85 6.978904622094797 1104 86-87 6.846355244426087 1104 88-89 6.471003563844061 1104 90-91 6.192401210984558 1104 92-93 5.917181399164255 1104 94-95 5.826329486704671 1104 96-97 5.393459879028805 1104 98-99 4.9413961529790065 1104 100-101 4.42687971530707 1105 1 8.089744625575783 1105 2 9.44584811514909 1105 3 8.726187117814817 1105 4 10.73349410015593 1105 5 10.817513329984827 1105 6 10.71547003093843 1105 7 9.994574349295906 1105 8 9.68854971522364 1105 9 9.659829247762808 1105 10-11 9.052675568130795 1105 12-13 8.638928543759533 1105 14-15 9.430820931211489 1105 16-17 10.616022232466463 1105 18-19 10.922740794336544 1105 20-21 11.43471592400137 1105 22-23 11.836389952074864 1105 24-25 11.781766413953786 1105 26-27 11.875625896190865 1105 28-29 11.880113435625482 1105 30-31 11.327037796147426 1105 32-33 11.307022190838993 1105 34-35 11.408891324482738 1105 36-37 11.29439514723066 1105 38-39 11.376003288651656 1105 40-41 11.018688491459422 1105 42-43 10.58458372337363 1105 44-45 10.99592078129439 1105 46-47 10.613965782690727 1105 48-49 10.534976829816845 1105 50-51 12.094082798019219 1105 52-53 10.660925381465557 1105 54-55 10.402282816756177 1105 56-57 10.326327973783535 1105 58-59 10.171758163811251 1105 60-61 10.375980298463716 1105 62-63 10.447477098928747 1105 64-65 10.178752640663824 1105 66-67 9.84907261794071 1105 68-69 9.544097622675277 1105 70-71 9.614220270455611 1105 72-73 9.380713501610185 1105 74-75 9.25308110575866 1105 76-77 8.819276727648775 1105 78-79 8.669461788230393 1105 80-81 8.417017130311907 1105 82-83 8.259334426800969 1105 84-85 8.278255848831657 1105 86-87 7.950810982981681 1105 88-89 7.429793421305831 1105 90-91 7.237643889481662 1105 92-93 7.176939349035335 1105 94-95 6.740963303453592 1105 96-97 6.427764052474641 1105 98-99 5.72036527780401 1105 100-101 4.959834417392981 1106 1 7.543248787317932 1106 2 8.878958483042066 1106 3 8.55021206312123 1106 4 10.414114966703572 1106 5 10.748071330336877 1106 6 10.2054177262637 1106 7 9.433273521976773 1106 8 9.008394058523361 1106 9 8.757812500208303 1106 10-11 8.22218483460803 1106 12-13 8.016484054610245 1106 14-15 8.511097919669265 1106 16-17 9.862133958269268 1106 18-19 11.131311971443193 1106 20-21 11.13080501797376 1106 22-23 11.20388687163128 1106 24-25 11.131585359310495 1106 26-27 11.203966605824487 1106 28-29 11.03107088778431 1106 30-31 10.809671009615876 1106 32-33 10.728288567482945 1106 34-35 10.708781937302415 1106 36-37 10.786297981238757 1106 38-39 10.562146950481814 1106 40-41 10.565193759646611 1106 42-43 10.329004348515081 1106 44-45 10.145664915878346 1106 46-47 9.893723496372925 1106 48-49 9.889482450054729 1106 50-51 11.594991214545484 1106 52-53 10.801943688101712 1106 54-55 10.27238868342955 1106 56-57 10.211810911898048 1106 58-59 10.14107946036367 1106 60-61 10.012236490783984 1106 62-63 9.936519521037528 1106 64-65 9.779882346198562 1106 66-67 9.812157587890667 1106 68-69 9.432899895916908 1106 70-71 9.05176899175719 1106 72-73 9.000750215468411 1106 74-75 8.738474145213488 1106 76-77 8.22508116348754 1106 78-79 7.985244975295046 1106 80-81 7.837051329522305 1106 82-83 7.531887674468635 1106 84-85 7.304716230555199 1106 86-87 6.90288179546439 1106 88-89 6.568658560743053 1106 90-91 6.296590377775976 1106 92-93 6.02705062220153 1106 94-95 5.964166587583646 1106 96-97 5.365385069901153 1106 98-99 4.918635828763348 1106 100-101 4.7866600517128965 1107 1 7.423789365983179 1107 2 8.282298764955904 1107 3 8.216967546230432 1107 4 10.028786136886325 1107 5 10.795107473404496 1107 6 9.854014165246806 1107 7 9.503780716108619 1107 8 8.349839728983014 1107 9 5.770869823385212 1107 10-11 7.755825368342599 1107 12-13 7.697725760721859 1107 14-15 8.457180461491358 1107 16-17 9.400980049823346 1107 18-19 10.638190674305175 1107 20-21 10.909573708610413 1107 22-23 10.283243056912827 1107 24-25 10.648689055119402 1107 26-27 10.66388622694399 1107 28-29 10.913041019337136 1107 30-31 10.898453492923611 1107 32-33 11.014111634680422 1107 34-35 10.847797302546972 1107 36-37 11.208236818056829 1107 38-39 11.107790112138186 1107 40-41 10.55343668306594 1107 42-43 10.414858501467442 1107 44-45 9.866318918365206 1107 46-47 10.330631822130737 1107 48-49 10.451554694393987 1107 50-51 11.923120949245547 1107 52-53 10.762568616264883 1107 54-55 10.799264800165702 1107 56-57 10.316728599953805 1107 58-59 10.410014995299878 1107 60-61 10.19596179197621 1107 62-63 10.406485196094739 1107 64-65 10.299541473916609 1107 66-67 10.574239889385922 1107 68-69 10.088690466745408 1107 70-71 9.753165499703652 1107 72-73 9.537074834341379 1107 74-75 9.212232804816633 1107 76-77 9.025130534980596 1107 78-79 8.562343524974786 1107 80-81 8.54786172731548 1107 82-83 8.396838355220545 1107 84-85 8.212776192102902 1107 86-87 7.741623135861245 1107 88-89 7.921124653759989 1107 90-91 7.48319437660569 1107 92-93 7.152151846823534 1107 94-95 6.8798170896577755 1107 96-97 6.445701909498654 1107 98-99 6.342534504875905 1107 100-101 5.608827982707744 1108 1 6.972829654262043 1108 2 8.032035650241735 1108 3 7.99281944821972 1108 4 9.442726473621882 1108 5 11.040244498163556 1108 6 9.25724980322147 1108 7 8.615957959922696 1108 8 6.060474755223979 1108 9 7.075600568259443 1108 10-11 5.933366909060325 1108 12-13 4.734994790229166 1108 14-15 6.916306824496083 1108 16-17 8.410270627175866 1108 18-19 8.049363499330362 1108 20-21 8.8156436785365 1108 22-23 9.463299730732626 1108 24-25 9.050572905195931 1108 26-27 7.509091930732584 1108 28-29 9.338059254693086 1108 30-31 9.923167367689981 1108 32-33 10.341728552475821 1108 34-35 9.835229488815525 1108 36-37 9.615651685544439 1108 38-39 9.101297011448256 1108 40-41 9.188053509844803 1108 42-43 8.693861690892088 1108 44-45 8.441963597487035 1108 46-47 9.167908190647733 1108 48-49 9.547437093333581 1108 50-51 10.925093508399886 1108 52-53 10.133989006277162 1108 54-55 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2313 50-51 -14.559184986827514 2313 52-53 -16.444337776429183 2313 54-55 -17.205872186135668 2313 56-57 -17.209241719680897 2313 58-59 -16.940156237576833 2313 60-61 -16.641081587019222 2313 62-63 -16.21851480390526 2313 64-65 -15.859419713298003 2313 66-67 -15.520794357189423 2313 68-69 -15.080830081199796 2313 70-71 -14.736560527693609 2313 72-73 -14.339066718170034 2313 74-75 -13.880232948608025 2313 76-77 -13.47829412255365 2313 78-79 -13.120590264979555 2313 80-81 -12.702994437068083 2313 82-83 -12.24471415619498 2313 84-85 -11.840020611550065 2313 86-87 -11.353411110714097 2313 88-89 -10.926192697838182 2313 90-91 -10.470858134809836 2313 92-93 -9.928612993359112 2313 94-95 -9.355913503949532 2313 96-97 -8.705839186391755 2313 98-99 -8.038744033936881 2313 100-101 -7.103786172543398 2314 1 -19.029977757304493 2314 2 -17.707998038697063 2314 3 -18.410886335048108 2314 4 -19.85991249325066 2314 5 -19.15009800604756 2314 6 -20.209341999136758 2314 7 -20.434575448274945 2314 8 -20.938402280149408 2314 9 -22.273079948304286 2314 10-11 -22.668261389648265 2314 12-13 -22.599648668501885 2314 14-15 -22.604748762252935 2314 16-17 -21.373277941044236 2314 18-19 -20.230816175009895 2314 20-21 -19.893508483170404 2314 22-23 -19.538103975050642 2314 24-25 -19.37699587638745 2314 26-27 -19.101239343832265 2314 28-29 -18.91601149207839 2314 30-31 -18.928601301596938 2314 32-33 -18.831208000022777 2314 34-35 -18.52748808558088 2314 36-37 -18.029491492445455 2314 38-39 -17.946148244026194 2314 40-41 -17.834405782687483 2314 42-43 -17.69187665834991 2314 44-45 -17.332881994876804 2314 46-47 -16.99042982170488 2314 48-49 -16.797874529350306 2314 50-51 -14.559184986827514 2314 52-53 -16.444337776429183 2314 54-55 -17.205872186135668 2314 56-57 -17.209241719680897 2314 58-59 -16.940156237576833 2314 60-61 -16.641081587019222 2314 62-63 -16.21851480390526 2314 64-65 -15.859419713298003 2314 66-67 -15.520794357189423 2314 68-69 -15.080830081199796 2314 70-71 -14.736560527693609 2314 72-73 -14.339066718170034 2314 74-75 -13.880232948608025 2314 76-77 -13.47829412255365 2314 78-79 -13.120590264979555 2314 80-81 -12.702994437068083 2314 82-83 -12.24471415619498 2314 84-85 -11.840020611550065 2314 86-87 -11.353411110714097 2314 88-89 -10.926192697838182 2314 90-91 -10.470858134809836 2314 92-93 -9.928612993359112 2314 94-95 -9.355913503949532 2314 96-97 -8.705839186391755 2314 98-99 -8.038744033936881 2314 100-101 -7.103786172543398 2315 1 -19.029977757304493 2315 2 -17.707998038697063 2315 3 -18.410886335048108 2315 4 -19.85991249325066 2315 5 -19.15009800604756 2315 6 -20.209341999136758 2315 7 -20.434575448274945 2315 8 -20.938402280149408 2315 9 -22.273079948304286 2315 10-11 -22.668261389648265 2315 12-13 -22.599648668501885 2315 14-15 -22.604748762252935 2315 16-17 -21.373277941044236 2315 18-19 -20.230816175009895 2315 20-21 -19.893508483170404 2315 22-23 -19.538103975050642 2315 24-25 -19.37699587638745 2315 26-27 -19.101239343832265 2315 28-29 -18.91601149207839 2315 30-31 -18.928601301596938 2315 32-33 -18.831208000022777 2315 34-35 -18.52748808558088 2315 36-37 -18.029491492445455 2315 38-39 -17.946148244026194 2315 40-41 -17.834405782687483 2315 42-43 -17.69187665834991 2315 44-45 -17.332881994876804 2315 46-47 -16.99042982170488 2315 48-49 -16.797874529350306 2315 50-51 -14.559184986827514 2315 52-53 -16.444337776429183 2315 54-55 -17.205872186135668 2315 56-57 -17.209241719680897 2315 58-59 -16.940156237576833 2315 60-61 -16.641081587019222 2315 62-63 -16.21851480390526 2315 64-65 -15.859419713298003 2315 66-67 -15.520794357189423 2315 68-69 -15.080830081199796 2315 70-71 -14.736560527693609 2315 72-73 -14.339066718170034 2315 74-75 -13.880232948608025 2315 76-77 -13.47829412255365 2315 78-79 -13.120590264979555 2315 80-81 -12.702994437068083 2315 82-83 -12.24471415619498 2315 84-85 -11.840020611550065 2315 86-87 -11.353411110714097 2315 88-89 -10.926192697838182 2315 90-91 -10.470858134809836 2315 92-93 -9.928612993359112 2315 94-95 -9.355913503949532 2315 96-97 -8.705839186391755 2315 98-99 -8.038744033936881 2315 100-101 -7.103786172543398 2316 1 -19.029977757304493 2316 2 -17.707998038697063 2316 3 -18.410886335048108 2316 4 -19.85991249325066 2316 5 -19.15009800604756 2316 6 -20.209341999136758 2316 7 -20.434575448274945 2316 8 -20.938402280149408 2316 9 -22.273079948304286 2316 10-11 -22.668261389648265 2316 12-13 -22.599648668501885 2316 14-15 -22.604748762252935 2316 16-17 -21.373277941044236 2316 18-19 -20.230816175009895 2316 20-21 -19.893508483170404 2316 22-23 -19.538103975050642 2316 24-25 -19.37699587638745 2316 26-27 -19.101239343832265 2316 28-29 -18.91601149207839 2316 30-31 -18.928601301596938 2316 32-33 -18.831208000022777 2316 34-35 -18.52748808558088 2316 36-37 -18.029491492445455 2316 38-39 -17.946148244026194 2316 40-41 -17.834405782687483 2316 42-43 -17.69187665834991 2316 44-45 -17.332881994876804 2316 46-47 -16.99042982170488 2316 48-49 -16.797874529350306 2316 50-51 -14.559184986827514 2316 52-53 -16.444337776429183 2316 54-55 -17.205872186135668 2316 56-57 -17.209241719680897 2316 58-59 -16.940156237576833 2316 60-61 -16.641081587019222 2316 62-63 -16.21851480390526 2316 64-65 -15.859419713298003 2316 66-67 -15.520794357189423 2316 68-69 -15.080830081199796 2316 70-71 -14.736560527693609 2316 72-73 -14.339066718170034 2316 74-75 -13.880232948608025 2316 76-77 -13.47829412255365 2316 78-79 -13.120590264979555 2316 80-81 -12.702994437068083 2316 82-83 -12.24471415619498 2316 84-85 -11.840020611550065 2316 86-87 -11.353411110714097 2316 88-89 -10.926192697838182 2316 90-91 -10.470858134809836 2316 92-93 -9.928612993359112 2316 94-95 -9.355913503949532 2316 96-97 -8.705839186391755 2316 98-99 -8.038744033936881 2316 100-101 -7.103786172543398 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 427151.0 3 16822.0 4 66622.0 5 35425.0 6 18514.0 7 19077.0 8 18611.0 9 17595.0 10 18540.0 11 19640.0 12 20491.0 13 21242.0 14 21997.0 15 22928.0 16 23892.0 17 24075.0 18 23397.0 19 24195.0 20 25737.0 21 27102.0 22 29043.0 23 31115.0 24 33184.0 25 35516.0 26 38595.0 27 41178.0 28 44537.0 29 48529.0 30 52722.0 31 57354.0 32 62434.0 33 67636.0 34 72812.0 35 71369.0 36 58065.0 37 27884.0 38 3941.0 39 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.37067795318809 21.364598283076283 14.671208019566897 22.593515744168727 2 14.437781623177482 21.31966693508318 42.564805455137325 21.67774598660202 3 18.20420708412596 27.072570629018816 31.02040655902652 23.7028157278287 4 11.882331129738573 17.901389277517325 40.85897279639094 29.357306796353168 5 12.048475687931012 38.70047728939212 36.47120743869688 12.77983958397999 6 28.293749590910082 39.80281259924218 18.885691590242608 13.017746219605126 7 24.915181074468627 33.85334369743616 24.074956037514657 17.15651919058056 8 23.506887974202108 37.75631142692901 21.433583944754194 17.303216654114692 9 24.839192916560737 17.42723701583605 22.06638127338981 35.66718879421341 10-11 23.201937042548558 27.87332141416953 29.981764393104015 18.942977150177896 12-13 23.94310377084979 25.375185486162984 30.164084737363016 20.517626005624212 14-15 21.17689090799687 27.213242373187313 27.683524881160114 23.926341837655695 16-17 20.65498162594744 30.367508560213878 28.297927274827963 20.67958253901072 18-19 20.388720635214458 29.16010726361973 30.509369380063262 19.941802721102555 20-21 21.084944095364065 28.403932690425034 30.492415659967115 20.018707554243782 22-23 20.702807581963814 28.2518595813566 30.312930133828626 20.732402702850955 24-25 20.679825172599838 28.42139110663149 30.429110833699742 20.46967288706894 26-27 20.636250330282977 28.632114465247433 30.119088784312257 20.61254642015733 28-29 20.668173236850983 28.727224927835614 30.225330320192967 20.379271515120436 30-31 20.89256004882494 28.483626087901616 30.165411207999266 20.45840265527418 32-33 20.68770281546497 28.684117810674657 30.027670468007955 20.600508905852415 34-35 20.826372575831822 28.646467838292818 30.071194213354868 20.45596537252049 36-37 20.877263325750413 28.544735947593818 29.979776559623613 20.598224167032157 38-39 20.88069473016623 28.601235993698133 30.050698948446087 20.46737032768955 40-41 20.865815233070105 28.647930627421324 29.98748002066841 20.498774118840156 42-43 20.76318149772356 28.666022103266215 29.82639885572378 20.744397543286443 44-45 20.903823287258824 28.840156933895628 29.69102740912935 20.564992369716197 46-47 20.958421447118344 28.78003955082046 29.346867722247506 20.91467127981369 48-49 20.912623027952325 28.80234414824297 29.361657542293834 20.92337528151087 50-51 21.13844745928296 29.06576737921398 29.00456007141699 20.791225090086066 52-53 20.774951228271103 29.177393211751802 29.109724257747665 20.937931302229426 54-55 20.61510116868119 29.11966915538247 29.28948864632589 20.97574102961045 56-57 20.677230595683792 29.270685036154948 29.20495614492146 20.847128223239807 58-59 20.756123711075166 29.078955772539437 29.229876824801025 20.935043691584372 60-61 20.712378782696295 29.290754298207837 29.220469515122804 20.776397403973064 62-63 20.655145965431522 29.058084309741567 29.24852075247484 21.03824897235207 64-65 20.498293552666407 29.038143060486547 29.245290384944223 21.21827300190282 66-67 20.483597139439546 29.081473613951697 29.059303602194518 21.375625644414235 68-69 20.49070125503678 29.07012550367787 29.108527517791806 21.330645723493546 70-71 20.63582646276544 28.861888899384596 29.13477294532873 21.367511692521234 72-73 20.57787616171212 29.069582128482995 28.972168005851085 21.380373703953808 74-75 20.459275826747234 29.210513950625405 28.84077773914351 21.48943248348385 76-77 20.482602468397523 29.03697948579312 28.914357466038815 21.566060579770543 78-79 20.32426596011479 29.16724259068171 28.855726726766974 21.652764722436533 80-81 20.479264196396418 29.205988998025607 28.652475444238863 21.662271361339112 82-83 20.579095507296504 29.032865980563326 28.834714478486994 21.55332403365317 84-85 20.607191258570204 29.146862674171718 28.601258830465255 21.644687236792823 86-87 20.450980807239095 29.22826741695421 28.696189352665296 21.624562423141395 88-89 20.597457688169065 29.103388813858277 28.605216721353493 21.693936776619164 90-91 20.57853061441179 29.064869645569978 28.679474942996748 21.67712479702148 92-93 20.54688569683562 28.98531884550712 28.525177033831422 21.942618423825838 94-95 20.409478592347078 28.98492104274694 28.624724506999033 21.98087585790695 96-97 20.364469180744095 28.839282269389845 28.613254289718128 22.18299426014794 98-99 20.234742936365556 28.83561226098779 28.338733142331822 22.590911660314823 100-101 20.436449088977845 28.987855696247717 28.28807432333644 22.287620891438 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 103959.0 1 94960.5 2 63242.0 3 31818.0 4 19744.0 5 15462.5 6 14426.5 7 14716.5 8 15138.5 9 14722.5 10 13421.0 11 11188.0 12 8635.5 13 6524.5 14 5056.0 15 4176.0 16 3960.0 17 4262.0 18 4864.0 19 5726.0 20 6680.5 21 7746.5 22 8844.0 23 9908.5 24 11049.0 25 12454.0 26 14283.5 27 16443.5 28 19126.0 29 22740.0 30 26939.5 31 31089.5 32 35245.0 33 38944.0 34 42014.0 35 44594.5 36 46978.5 37 49151.0 38 50137.0 39 50032.5 40 49540.5 41 49097.0 42 48400.0 43 48080.0 44 47780.5 45 47216.5 46 46428.0 47 45032.5 48 43347.5 49 41136.5 50 38251.0 51 35091.5 52 32224.5 53 28935.0 54 25246.0 55 21458.0 56 17631.0 57 14135.0 58 11079.0 59 8519.5 60 6433.5 61 4848.0 62 3556.5 63 2457.5 64 1641.0 65 1066.0 66 682.0 67 440.0 68 280.5 69 180.5 70 115.0 71 74.5 72 50.0 73 34.5 74 26.0 75 17.5 76 8.5 77 9.0 78 5.0 79 3.0 80 3.0 81 1.5 82 1.5 83 1.0 84 1.0 85 1.5 86 1.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.932469580514272 2 30.05735184506134 3 23.778127688742217 4 30.1936014226517 5 33.403974605091925 6 25.853447222162306 7 24.768183986061082 8 20.681116072177524 9 12.327890540181043 10-11 16.051027084546636 12-13 16.094825871362183 14-15 20.340132630635424 16-17 23.76791196623599 18-19 27.819149956021466 20-21 29.92307950408509 22-23 29.546296096575425 24-25 27.981523023422465 26-27 29.47730252199526 28-29 26.385773243315143 30-31 28.333561015125262 32-33 27.29835781511759 34-35 25.292151688799734 36-37 28.346772550683163 38-39 28.48308204453456 40-41 25.43954569094234 42-43 26.316599919951877 44-45 24.97004186948321 46-47 28.374663570194297 48-49 23.65760612967317 50-51 29.22502510491337 52-53 22.83459636811592 54-55 23.966594287823337 56-57 26.117528142667805 58-59 25.422799095983457 60-61 24.939274869442468 62-63 24.772917370682734 64-65 23.263387090202734 66-67 23.7872050022888 68-69 24.79655433566048 70-71 23.25044517781948 72-73 23.116562292539946 74-75 20.303134339447187 76-77 22.65056357235128 78-79 21.757002413426996 80-81 22.798137323276986 82-83 23.69541329038536 84-85 20.575273997061707 86-87 22.165331732370838 88-89 20.98716833353705 90-91 22.126446078960047 92-93 22.10682350347155 94-95 21.120032642379012 96-97 20.024343976857946 98-99 19.443066370440672 100-101 21.112722858532916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1668996.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.69741355152105 #Duplication Level Percentage of deduplicated Percentage of total 1 90.57531876224772 73.09193955717947 2 6.552687095184481 10.575698007876346 3 1.4549745318818665 3.5223804451860516 4 0.5714925102701773 1.8447186977147756 5 0.27998035091520795 1.129684508205226 6 0.14575577291509084 0.7057268330670406 7 0.09868695776725289 0.557464757015985 8 0.06485285516771189 0.41867661387725963 9 0.04891408327837935 0.3552516006128027 >10 0.20013906373105095 2.5705319729778715 >50 0.005193647615336526 0.29185493187567524 >100 0.0017075250833049787 0.18864188580798913 >500 1.4842197119187148E-4 0.09120690487729925 >1k 7.421098559593574E-5 0.07479804608781797 >5k 0.0 0.0 >10k+ 7.421098559593574E-5 4.58142523763831 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 76502 4.583713801590897 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.9958130516789735E-5 0.0 12-13 0.0 0.0 0.0 5.991626103357947E-5 0.0 14-15 0.0 0.0 0.0 5.991626103357947E-5 0.0 16-17 0.0 0.0 0.0 5.991626103357947E-5 2.9958130516789735E-5 18-19 0.0 0.0 0.0 5.991626103357947E-5 5.991626103357947E-5 20-21 0.0 0.0 0.0 5.991626103357947E-5 5.991626103357947E-5 22-23 0.0 0.0 0.0 1.7974878310073842E-4 5.991626103357947E-5 24-25 0.0 0.0 0.0 2.696231746511076E-4 5.991626103357947E-5 26-27 0.0 0.0 0.0 3.295394356846871E-4 5.991626103357947E-5 28-29 0.0 0.0 0.0 5.991626103357947E-4 5.991626103357947E-5 30-31 0.0 0.0 0.0 0.0013181577427387485 5.991626103357947E-5 32-33 0.0 0.0 0.0 0.004403845185968092 5.991626103357947E-5 34-35 0.0 0.0 0.0 0.01012584811467493 5.991626103357947E-5 36-37 0.0 0.0 0.0 0.01881370596454395 5.991626103357947E-5 38-39 0.0 0.0 0.0 0.03304381796001908 5.991626103357947E-5 40-41 0.0 5.991626103357947E-5 0.0 0.046255353517923356 5.991626103357947E-5 42-43 0.0 5.991626103357947E-5 0.0 0.05997617729461305 5.991626103357947E-5 44-45 0.0 5.991626103357947E-5 0.0 0.07654302347039776 5.991626103357947E-5 46-47 0.0 5.991626103357947E-5 0.0 0.09721413352698269 5.991626103357947E-5 48-49 0.0 5.991626103357947E-5 0.0 0.12088105663524658 5.991626103357947E-5 50-51 0.0 5.991626103357947E-5 0.0 0.14733408588157193 5.991626103357947E-5 52-53 0.0 5.991626103357947E-5 0.0 0.17516518913166956 5.991626103357947E-5 54-55 2.9958130516789735E-5 5.991626103357947E-5 0.0 0.20769971887290323 5.991626103357947E-5 56-57 5.991626103357947E-5 5.991626103357947E-5 0.0 0.24565667023767584 5.991626103357947E-5 58-59 5.991626103357947E-5 5.991626103357947E-5 0.0 0.28547102569448934 5.991626103357947E-5 60-61 5.991626103357947E-5 5.991626103357947E-5 0.0 0.3282811942029819 5.991626103357947E-5 62-63 5.991626103357947E-5 5.991626103357947E-5 0.0 0.37555512415847614 5.991626103357947E-5 64-65 5.991626103357947E-5 5.991626103357947E-5 0.0 0.42127123132709726 5.991626103357947E-5 66-67 5.991626103357947E-5 5.991626103357947E-5 0.0 0.4749262430826676 5.991626103357947E-5 68-69 5.991626103357947E-5 5.991626103357947E-5 0.0 0.5355914573791669 5.991626103357947E-5 70-71 5.991626103357947E-5 5.991626103357947E-5 0.0 0.605393901483287 5.991626103357947E-5 72-73 5.991626103357947E-5 5.991626103357947E-5 0.0 0.6759452988503267 5.991626103357947E-5 74-75 5.991626103357947E-5 5.991626103357947E-5 0.0 0.7473654820023534 5.991626103357947E-5 76-77 5.991626103357947E-5 5.991626103357947E-5 0.0 0.8240283379948184 5.991626103357947E-5 78-79 5.991626103357947E-5 5.991626103357947E-5 0.0 0.9143820596334563 5.991626103357947E-5 80-81 5.991626103357947E-5 5.991626103357947E-5 0.0 1.0134835553829968 5.991626103357947E-5 82-83 5.991626103357947E-5 5.991626103357947E-5 0.0 1.118756426018996 5.991626103357947E-5 84-85 5.991626103357947E-5 5.991626103357947E-5 0.0 1.2233702177836256 5.991626103357947E-5 86-87 5.991626103357947E-5 5.991626103357947E-5 0.0 1.3266958099360333 5.991626103357947E-5 88-89 5.991626103357947E-5 5.991626103357947E-5 0.0 1.4476367828323136 5.991626103357947E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1280 0.0 54.19238 1 GTATCAA 2555 0.0 43.23764 1 ATCAACG 2845 0.0 36.569 3 TCAACGC 2905 0.0 36.330276 4 CAACGCA 2995 0.0 35.392605 5 AACGCAG 3215 0.0 33.044056 6 TATCAAC 3185 0.0 32.90137 2 GTGGTAT 630 0.0 29.361374 1 ACGCAGA 3700 0.0 28.59959 7 CGCAGAG 3790 0.0 27.906672 8 GTACATG 2885 0.0 24.756168 1 TAACGCA 145 1.4538149E-5 24.378033 4 TACATGG 2885 0.0 23.859003 2 ACATGGG 2935 0.0 23.40232 3 GCAGAGT 4160 0.0 23.26771 9 GAGTACT 2640 0.0 21.563004 12-13 TGGTATC 760 0.0 20.952766 2 GTACTAG 305 1.717126E-9 20.216028 1 GCCGATA 130 0.0048119216 19.756952 2 AGAGTAC 3745 0.0 19.545378 10-11 >>END_MODULE