FastQCFastQC Report
Sat 18 Jun 2016
SRR1930997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1668996
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36250.21719644624672557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34240.2051532777789761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.1121033243938272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23350.055.7697331
GTATCAA41550.037.9753651
CAACGCA49900.030.9925675
ATCAACG49950.030.9193693
TCAACGC50500.030.5807884
TATCAAC52900.029.1968842
AACGCAG53150.029.1866976
ACGCAGA60250.025.5110597
CGCAGAG60350.025.3901798
GTGGTAT11800.022.9576661
GCAGAGT69950.021.2952339
GTACATG43750.020.7486671
TACATGG43300.020.4142722
GAGTACT49150.020.31744412-13
ACATGGG41250.020.1602953
TATACCG1456.200583E-419.6301575
TAACGCA1951.02975955E-519.4944974
GCGTGCG2953.0668161E-919.2974419
TGGTATC11950.019.0889552
CAGAGTA69750.019.07783910-11
Produced by FastQC (version 0.11.5)