##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930997_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1668996 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.376018276856264 31.0 31.0 34.0 30.0 34.0 2 31.54578321338098 31.0 31.0 34.0 30.0 34.0 3 31.611641370021257 31.0 31.0 34.0 30.0 34.0 4 35.378038653178315 37.0 35.0 37.0 33.0 37.0 5 35.25638168096269 37.0 35.0 37.0 33.0 37.0 6 35.28075921092681 37.0 35.0 37.0 32.0 37.0 7 35.167380269335574 37.0 35.0 37.0 32.0 37.0 8 35.17006931113077 37.0 35.0 37.0 32.0 37.0 9 36.678784730460706 39.0 35.0 39.0 32.0 39.0 10-11 36.61904851779153 39.0 35.0 39.0 32.0 39.0 12-13 36.51291345215927 38.0 35.0 39.0 32.0 39.0 14-15 37.58848583220092 39.0 36.0 41.0 32.0 41.0 16-17 37.541459655984795 39.0 36.0 41.0 32.0 41.0 18-19 37.5647287351198 39.0 36.0 41.0 32.0 41.0 20-21 37.553015705250345 39.0 36.0 41.0 32.0 41.0 22-23 37.45927162198112 39.0 36.0 41.0 32.0 41.0 24-25 37.339726997548226 39.0 36.0 41.0 31.5 41.0 26-27 37.099101495749544 39.0 36.0 40.0 31.0 41.0 28-29 36.91623437084331 39.0 36.0 40.0 31.0 41.0 30-31 36.70300228400787 38.5 35.5 40.0 30.0 41.0 32-33 36.549743079072684 38.0 35.0 40.0 30.0 41.0 34-35 36.63779661545025 38.5 35.0 40.0 30.0 41.0 36-37 36.71154484492473 39.0 35.0 40.0 30.0 41.0 38-39 36.69856188990267 39.0 35.0 40.0 30.0 41.0 40-41 36.59731629075205 39.0 35.0 40.0 30.0 41.0 42-43 36.47396069253611 39.0 35.0 40.0 30.0 41.0 44-45 36.32706968740489 39.0 35.0 40.0 30.0 41.0 46-47 36.11096731208463 38.5 35.0 40.0 29.5 41.0 48-49 35.91855013433226 38.5 35.0 40.0 29.0 41.0 50-51 35.672915033948556 38.0 35.0 40.0 28.0 41.0 52-53 35.47515452403721 38.0 34.0 40.0 27.0 41.0 54-55 35.34175096884594 38.0 34.0 40.0 27.0 41.0 56-57 35.18724670400648 38.0 34.0 40.0 27.0 41.0 58-59 35.00459857303433 38.0 34.0 40.0 26.5 41.0 60-61 34.71026593233297 38.0 33.5 40.0 26.0 41.0 62-63 34.46540644794835 37.5 33.0 40.0 26.0 41.0 64-65 34.1759090495124 37.0 33.0 40.0 26.0 41.0 66-67 33.869961641609684 36.5 32.5 39.5 25.0 41.0 68-69 33.53810764076127 36.0 32.0 39.0 24.5 40.5 70-71 33.175004014389486 36.0 32.0 39.0 23.5 40.0 72-73 32.75239155755916 35.0 31.0 38.5 22.5 40.0 74-75 32.235570366855285 35.0 31.0 38.0 22.0 40.0 76-77 30.83050888078821 33.5 29.5 36.0 20.5 39.0 78-79 31.572345889384998 34.0 30.5 36.0 21.5 39.0 80-81 31.52829725176094 34.5 31.0 36.0 22.0 39.0 82-83 31.243463735083846 34.0 31.0 36.0 21.0 37.5 84-85 30.892311605300435 34.0 31.0 35.5 20.0 37.0 86-87 30.538795479437937 34.0 30.0 35.0 19.5 37.0 88-89 30.181422843434017 34.0 30.0 35.0 18.0 36.0 90-91 29.837371090164385 34.0 30.0 35.0 16.0 36.0 92-93 29.513503926911746 34.0 29.0 35.0 9.0 36.0 94-95 29.14160009970066 34.0 29.0 35.0 4.5 35.0 96-97 28.721621861286664 34.0 29.0 35.0 2.0 35.0 98-99 28.242510467370803 34.0 29.0 35.0 2.0 35.0 100-101 27.1214871096156 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29365571079065944 1101 2 0.35473674523066023 1101 3 0.293119711819287 1101 4 -0.04183291807384393 1101 5 0.19608303748171352 1101 6 0.21597619477095265 1101 7 0.33180459495692816 1101 8 0.39272968267003705 1101 9 0.4573621829368477 1101 10-11 0.3893541555196336 1101 12-13 0.4212442516854722 1101 14-15 0.4553499855550385 1101 16-17 0.60575958367113 1101 18-19 0.43066332179454037 1101 20-21 0.43251969203535623 1101 22-23 0.4391649748684969 1101 24-25 0.45812343002175737 1101 26-27 0.38792202311273627 1101 28-29 0.5647586180879003 1101 30-31 0.3103942727275637 1101 32-33 0.30334645945898586 1101 34-35 0.27609392019216017 1101 36-37 0.5965533736511546 1101 38-39 0.7152292065547385 1101 40-41 0.6783886175493521 1101 42-43 0.6811820561847242 1101 44-45 0.706711735155892 1101 46-47 0.7045061565971338 1101 48-49 0.5266767417694282 1101 50-51 0.4983074003170529 1101 52-53 0.30436718387242934 1101 54-55 0.3995753799417159 1101 56-57 0.5301523097450698 1101 58-59 0.6197665206958689 1101 60-61 0.332953873180422 1101 62-63 0.07407871019604784 1101 64-65 0.05500308883322447 1101 66-67 0.04605980950725552 1101 68-69 0.24064386295727758 1101 70-71 0.23352171046275316 1101 72-73 0.20531216609980873 1101 74-75 0.2820977130941529 1101 76-77 0.17966245964562688 1101 78-79 0.29736244695990166 1101 80-81 0.2449152030621775 1101 82-83 0.13462080847345703 1101 84-85 0.1606396543132007 1101 86-87 0.21014004982424694 1101 88-89 0.47945041344955897 1101 90-91 0.41747318055879745 1101 92-93 0.36256332987987605 1101 94-95 0.36799949550441013 1101 96-97 0.562781557954505 1101 98-99 0.525388784644953 1101 100-101 0.6064003815312304 1102 1 0.3788419989251324 1102 2 0.35378545147114693 1102 3 0.46624690393099044 1102 4 0.1801328684392942 1102 5 0.287580952907561 1102 6 0.27525420416808544 1102 7 0.3341621490565885 1102 8 0.3622551938870302 1102 9 0.5049847757500245 1102 10-11 0.35151334419013125 1102 12-13 0.3696510414412799 1102 14-15 0.46863500970962946 1102 16-17 0.5163462423965655 1102 18-19 0.45964055692127204 1102 20-21 0.46554612972975207 1102 22-23 0.4387596410057526 1102 24-25 0.5450303190430006 1102 26-27 0.44132269150000525 1102 28-29 0.5920028441483893 1102 30-31 0.4716633813239497 1102 32-33 0.5999102209572271 1102 34-35 0.574920106414119 1102 36-37 0.5552754966079476 1102 38-39 0.6384639362218678 1102 40-41 0.7612628482422323 1102 42-43 0.7168431639869794 1102 44-45 0.7522828422963812 1102 46-47 0.7539155272542786 1102 48-49 0.773041145184159 1102 50-51 0.6898524668248953 1102 52-53 0.6979422285418764 1102 54-55 0.8075521849182294 1102 56-57 0.9040852063104765 1102 58-59 0.9169755075266508 1102 60-61 0.8272709269822869 1102 62-63 0.48997606973544805 1102 64-65 0.5780202617536148 1102 66-67 0.4957198253897275 1102 68-69 0.7298115222783039 1102 70-71 0.8181248141620472 1102 72-73 0.6213832401518289 1102 74-75 0.5200948674849997 1102 76-77 0.5842228792075375 1102 78-79 0.6265307680504932 1102 80-81 0.6808765888076636 1102 82-83 0.5908364791769216 1102 84-85 0.693517193853932 1102 86-87 0.7709401292365925 1102 88-89 0.7657815629433067 1102 90-91 0.7053222640079433 1102 92-93 0.7075065831390894 1102 94-95 0.9182857108442306 1102 96-97 0.928549872427407 1102 98-99 0.9479286562616558 1102 100-101 0.9958641827356516 1103 1 0.2389697636197603 1103 2 0.3420721637740911 1103 3 0.30814723846572534 1103 4 0.22402859432902034 1103 5 0.2057616776296527 1103 6 0.22911218587417892 1103 7 0.28170821440480864 1103 8 0.20303326146360945 1103 9 0.307839645635994 1103 10-11 0.29457930999387116 1103 12-13 0.31128647736338877 1103 14-15 0.3634303076537435 1103 16-17 0.46122292315926927 1103 18-19 0.41543252358597016 1103 20-21 0.557743472865603 1103 22-23 0.39688969314139655 1103 24-25 0.29120244264922235 1103 26-27 0.3750735953627782 1103 28-29 0.35157271098162113 1103 30-31 0.3080053115527761 1103 32-33 0.48414298566592606 1103 34-35 0.5460659582115781 1103 36-37 0.3915581657955727 1103 38-39 0.4394621946154871 1103 40-41 0.5553198274695603 1103 42-43 0.5975786480213188 1103 44-45 0.7388785926642569 1103 46-47 0.5424380176107348 1103 48-49 0.49494244337024185 1103 50-51 0.2789650705405151 1103 52-53 0.37580395653053245 1103 54-55 0.2803161972529651 1103 56-57 0.32321023901835844 1103 58-59 0.42314533806469257 1103 60-61 0.24153092715110347 1103 62-63 0.2358728141413522 1103 64-65 0.2811957743176592 1103 66-67 0.2510126980273668 1103 68-69 0.1855973342354602 1103 70-71 0.14380749432264395 1103 72-73 0.26440105855770923 1103 74-75 0.2545044657338238 1103 76-77 0.32346286778440003 1103 78-79 0.4808877031046137 1103 80-81 0.4456526348265868 1103 82-83 0.4096900716972982 1103 84-85 0.19661195665501907 1103 86-87 0.16981371224706976 1103 88-89 0.2491638525789206 1103 90-91 0.373760255505303 1103 92-93 0.2919373887516201 1103 94-95 0.2198920543430276 1103 96-97 0.4709867567807997 1103 98-99 0.3069063698897949 1103 100-101 0.6344402366325994 1104 1 0.18464869448700583 1104 2 0.376170773607587 1104 3 0.3265904860340214 1104 4 0.1258838052761746 1104 5 0.2544389374666878 1104 6 0.22005419513833147 1104 7 0.2963810979933328 1104 8 0.29051944960963993 1104 9 0.38194874703540904 1104 10-11 0.3567816407272275 1104 12-13 0.35744817675212914 1104 14-15 0.4388678644660544 1104 16-17 0.3184211103364234 1104 18-19 0.3712223439094018 1104 20-21 0.3679415539024049 1104 22-23 0.3496980580900413 1104 24-25 0.328913056412631 1104 26-27 0.43571154652732247 1104 28-29 0.3890972389291889 1104 30-31 0.44322812465321704 1104 32-33 0.2648428928611537 1104 34-35 0.2773065008337312 1104 36-37 0.2767330116199105 1104 38-39 0.33295494033040285 1104 40-41 0.30867328437930297 1104 42-43 0.5515775199398476 1104 44-45 0.46858775448203005 1104 46-47 0.34394075412681957 1104 48-49 0.4032431294723864 1104 50-51 0.336329170062065 1104 52-53 0.35198276084581437 1104 54-55 0.2844769332138597 1104 56-57 0.27530964082219356 1104 58-59 0.410289257583095 1104 60-61 0.38911909168498227 1104 62-63 0.29864547918960227 1104 64-65 0.17730239638723333 1104 66-67 0.3722224327309078 1104 68-69 0.15115955153471106 1104 70-71 0.3529373339146318 1104 72-73 0.37390592740633366 1104 74-75 0.413254547380248 1104 76-77 0.3909861642316912 1104 78-79 0.4462376396925549 1104 80-81 0.3249152587250421 1104 82-83 0.38405513464669383 1104 84-85 0.44180859527160266 1104 86-87 0.4772417681367713 1104 88-89 0.5689230704605777 1104 90-91 0.34858917222050323 1104 92-93 0.25708133820703694 1104 94-95 0.5160962779127196 1104 96-97 0.5227823998552772 1104 98-99 0.6752145946787422 1104 100-101 0.8199589499937652 1105 1 0.28916895902325734 1105 2 0.3591315228927314 1105 3 0.3480664891258627 1105 4 0.1915574024610862 1105 5 0.27423760289373433 1105 6 0.25746237230302427 1105 7 0.2562091142957712 1105 8 0.2780689758335342 1105 9 0.39534717841220157 1105 10-11 0.334904968457046 1105 12-13 0.33634767432784685 1105 14-15 0.3971736081566988 1105 16-17 0.47217959668529375 1105 18-19 0.47873546531824474 1105 20-21 0.43493151487601267 1105 22-23 0.4922633137885981 1105 24-25 0.38874705234783846 1105 26-27 0.23045288180296808 1105 28-29 0.42503668991106736 1105 30-31 0.38058859988876037 1105 32-33 0.507486086485045 1105 34-35 0.6084597456459804 1105 36-37 0.5335440498192838 1105 38-39 0.5406287685926543 1105 40-41 0.571642161911015 1105 42-43 0.4575609702082204 1105 44-45 0.4603193074838785 1105 46-47 0.31478362559377615 1105 48-49 0.4195660066689797 1105 50-51 0.3849236523137023 1105 52-53 0.35693555215190287 1105 54-55 0.4121868554853876 1105 56-57 0.44670010476061606 1105 58-59 0.7000239172913112 1105 60-61 0.658413373399334 1105 62-63 0.34471010381731304 1105 64-65 0.42108999712883843 1105 66-67 0.4709510682125 1105 68-69 0.4799859759710685 1105 70-71 0.3438232925207032 1105 72-73 0.30376855125567204 1105 74-75 0.5986500206968941 1105 76-77 0.38422647655094977 1105 78-79 0.596023724242535 1105 80-81 0.4846842894975154 1105 82-83 0.39090975322044486 1105 84-85 0.5201145889581937 1105 86-87 0.5087608516110933 1105 88-89 0.684024075579412 1105 90-91 0.7041957367086482 1105 92-93 0.4410504833019857 1105 94-95 0.528542190951967 1105 96-97 0.6435616912721756 1105 98-99 0.5908249158627186 1105 100-101 0.6195996990627002 1106 1 0.17740543650458562 1106 2 0.1234587288675364 1106 3 0.14432343584047658 1106 4 6.579129345922752E-4 1106 5 0.03786699409749161 1106 6 0.2454423557063521 1106 7 0.1351728279276756 1106 8 0.11492491165972041 1106 9 0.1721281777332493 1106 10-11 0.11131820053389418 1106 12-13 0.16284326112091918 1106 14-15 0.17245524871293583 1106 16-17 0.25147210052013236 1106 18-19 0.2264534224183521 1106 20-21 0.07721343204350717 1106 22-23 0.0936350541763531 1106 24-25 0.24258943422225343 1106 26-27 0.2544583385887478 1106 28-29 0.2630713669045548 1106 30-31 0.14524271008062328 1106 32-33 0.15184258810698736 1106 34-35 0.21226755111282358 1106 36-37 0.19584885028197618 1106 38-39 0.2311730641140599 1106 40-41 0.1153341678455817 1106 42-43 0.07067611593205925 1106 44-45 0.1529947670357643 1106 46-47 0.05320995898578218 1106 48-49 0.08449232748774449 1106 50-51 0.010266273079665211 1106 52-53 0.04247884181418726 1106 54-55 0.07330034154166754 1106 56-57 0.09296773872848263 1106 58-59 0.08971260389075297 1106 60-61 0.17462027862225682 1106 62-63 -0.0498790027283178 1106 64-65 -0.027290569672778986 1106 66-67 -0.12494188322965272 1106 68-69 -0.08297451891161955 1106 70-71 -0.14165461629816178 1106 72-73 -0.08246878120591106 1106 74-75 0.194071475672132 1106 76-77 0.2907287271607544 1106 78-79 0.10124790509600956 1106 80-81 0.05249176806014333 1106 82-83 0.13835109905996035 1106 84-85 0.2056421620715767 1106 86-87 0.11228261588800592 1106 88-89 -0.012250473669961792 1106 90-91 -0.04393085066279312 1106 92-93 0.02207243617748489 1106 94-95 0.1670144166667562 1106 96-97 0.08043774428095318 1106 98-99 0.12940590009587893 1106 100-101 0.3713568092294217 1107 1 0.10311570682004145 1107 2 0.13280404719611383 1107 3 0.0410103213090629 1107 4 0.02206920620648134 1107 5 -0.03758779833325576 1107 6 -0.010299366078754701 1107 7 0.022667211593812908 1107 8 0.13315056811772052 1107 9 0.06461848629201938 1107 10-11 0.10683231712375374 1107 12-13 0.06859138291437716 1107 14-15 0.17162455375039798 1107 16-17 0.009931213609590372 1107 18-19 0.002940967218407309 1107 20-21 0.16481321992894493 1107 22-23 0.09525987262509972 1107 24-25 0.061508381381692345 1107 26-27 -0.04255494730615794 1107 28-29 0.07406475269183233 1107 30-31 0.09424378632692054 1107 32-33 0.3109650816547358 1107 34-35 0.002009643837190822 1107 36-37 0.08423205143554924 1107 38-39 0.14744332209970423 1107 40-41 -0.024696055867359235 1107 42-43 0.0492210675009801 1107 44-45 0.1328086182212047 1107 46-47 0.08312934500293778 1107 48-49 0.1435866087537434 1107 50-51 -0.034077948104624056 1107 52-53 -0.06370905198224364 1107 54-55 0.15824869739258673 1107 56-57 0.16684081480760682 1107 58-59 0.15406272875622307 1107 60-61 0.2008936190609063 1107 62-63 -0.1036064198416824 1107 64-65 0.007827190481030755 1107 66-67 -0.003312336611472233 1107 68-69 0.016192304489941023 1107 70-71 0.20875498423682615 1107 72-73 0.0724453803146261 1107 74-75 0.17404728625278665 1107 76-77 0.06707063911753863 1107 78-79 0.11887078525650452 1107 80-81 0.17962336268099932 1107 82-83 0.2736792758151303 1107 84-85 0.21748152447401026 1107 86-87 0.2618697500426279 1107 88-89 0.5208565083473182 1107 90-91 0.4911405420835173 1107 92-93 0.4356102824309964 1107 94-95 0.4466549451768351 1107 96-97 0.44917226670971644 1107 98-99 0.6386382386850542 1107 100-101 0.8600359781164997 1108 1 0.0921815438260829 1108 2 0.13711803887386864 1108 3 0.08773289264167161 1108 4 0.025772361531977594 1108 5 0.15248037433666184 1108 6 0.18312295835546877 1108 7 0.0884821044635018 1108 8 0.1795984874283576 1108 9 0.23630836858583848 1108 10-11 0.21486173854059132 1108 12-13 0.21607647414884212 1108 14-15 0.2121060825205916 1108 16-17 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-0.6890016585085021 2316 86-87 -0.5897585864462158 2316 88-89 -0.8701321874985446 2316 90-91 -1.0315768169393884 2316 92-93 -1.0475378966535338 2316 94-95 -1.2054476460545587 2316 96-97 -1.0882994265003667 2316 98-99 -1.3701902314489587 2316 100-101 -1.6003786003443565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 25.0 5 30.0 6 64.0 7 191.0 8 488.0 9 900.0 10 1689.0 11 2660.0 12 3852.0 13 5075.0 14 6195.0 15 7825.0 16 9289.0 17 10806.0 18 9558.0 19 7716.0 20 7522.0 21 7915.0 22 9037.0 23 10388.0 24 12919.0 25 16505.0 26 21129.0 27 27505.0 28 35043.0 29 44141.0 30 54846.0 31 66637.0 32 80524.0 33 98147.0 34 122248.0 35 153437.0 36 201655.0 37 281368.0 38 290620.0 39 61022.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.49013519578312 22.440055293475453 15.395649743276014 22.67415976746541 2 14.570756263043952 22.252628563998424 41.300946684507004 21.875668488450618 3 17.934008887014127 27.229423015161608 31.72158336489006 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30.737495824576804 20.43586175492101 24-25 20.36824221696686 28.71368372236266 30.637609849830703 20.280464210839774 26-27 20.3166362083945 28.84846759938884 30.541328380119236 20.293567812097425 28-29 20.358317752585613 28.857062654827597 30.522767164761277 20.261852427825513 30-31 20.650224551122456 28.63393822821824 30.487001800603398 20.228835420055905 32-33 20.42013382195627 28.79925807621199 30.413901182703228 20.36670691912851 34-35 20.589460223255056 28.800237780776673 30.466351143720743 20.14395085224753 36-37 20.60276426734333 28.71626489871793 30.51158568644574 20.169385147493003 38-39 20.47825331487906 28.804816086375588 30.5095596738147 20.207370924930647 40-41 20.536083956727413 28.75829486115563 30.434583317105613 20.27103786501134 42-43 20.51838571041637 28.791296239923874 30.24497901481068 20.445339034849077 44-45 20.496962891368163 28.942353958427837 30.191981697652942 20.368701452551058 46-47 20.633272751132996 28.962726067184523 29.86642553632625 20.537575645356235 48-49 20.584505098982 29.009592743586985 29.82852787324647 20.57737428418454 50-51 20.677932116738365 29.118464924340024 29.683168221729417 20.520434737192193 52-53 20.735489641102912 29.154933421550737 29.54489355276114 20.56468338458521 54-55 20.61690685421431 29.119893617531297 29.6521954867753 20.611004041479095 56-57 20.641989364092577 29.188405362894166 29.561351209647217 20.60825406336604 58-59 20.630862215116156 29.155811059550203 29.642361992335882 20.57096473299776 60-61 20.67980851140413 29.280807947496612 29.6137233493707 20.425660191728557 62-63 20.64502528624765 29.21009792283708 29.61669586023842 20.528180930676854 64-65 20.485443540903496 29.04934198474291 29.618074262537007 20.847140211816587 66-67 20.505738620220292 29.183588393132275 29.490867523680404 20.81980546296702 68-69 20.54502420197454 29.1247795964005 29.5741311420563 20.756065059568662 70-71 20.548801233344875 29.05008841994504 29.54544430834703 20.855666038363054 72-73 20.60130987241241 29.21530979019657 29.41556838002683 20.767811957364184 74-75 20.500668886374037 29.322608719947052 29.375422654173576 20.801299739505332 76-77 20.45574434650453 29.193746590459078 29.388929203744407 20.96157985929198 78-79 20.352905330161164 29.205318866228115 29.465132352980827 20.976643450629894 80-81 20.540828300615548 29.295735069387547 29.249180120617464 20.914256509379445 82-83 20.50879690544495 29.251028762201948 29.421071111015245 20.81910322133786 84-85 20.577251233675813 29.291412322138576 29.20333541841922 20.928001025766388 86-87 20.415806868320836 29.329788687330588 29.26489937663122 20.98950506771736 88-89 20.511667507492536 29.182130073565272 29.192196017481187 21.114006401461 90-91 20.52418296580626 29.16243733904088 29.315741727195487 20.997637967957374 92-93 20.510799896805683 29.17924260043693 29.25199399651165 21.057963506245745 94-95 20.311010009589864 29.128053305387606 29.333931314278832 21.227005370743694 96-97 20.29526143120218 29.010952995379558 29.37209836695186 21.321687206466404 98-99 20.226291734671623 29.123047628634218 29.192520533302655 21.4581401033915 100-101 20.26802341048151 29.02805039676548 29.218733897504844 21.48519229524816 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 179.0 1 122.0 2 96.5 3 147.0 4 142.0 5 137.5 6 248.0 7 510.5 8 804.0 9 1018.5 10 982.0 11 856.5 12 902.0 13 1020.5 14 1161.5 15 1306.5 16 1455.5 17 1605.5 18 1817.0 19 2103.0 20 2471.5 21 2947.5 22 3578.0 23 4474.0 24 5765.5 25 7747.5 26 10518.0 27 13684.5 28 17944.0 29 24011.5 30 31179.5 31 38065.0 32 44945.5 33 51101.0 34 55924.0 35 59564.0 36 64032.5 37 68748.0 38 70079.5 39 69546.5 40 68680.0 41 67642.0 42 67225.5 43 67565.0 44 67575.0 45 67792.5 46 66814.5 47 64795.0 48 62825.5 49 60075.0 50 56416.5 51 51589.5 52 46130.0 53 40734.5 54 35634.0 55 29599.5 56 23334.0 57 17814.0 58 13249.0 59 9799.0 60 6998.0 61 5061.0 62 3649.0 63 2241.5 64 1204.5 65 707.5 66 394.0 67 212.0 68 128.0 69 80.0 70 55.5 71 39.5 72 23.5 73 18.5 74 15.5 75 8.0 76 7.0 77 5.0 78 2.0 79 1.0 80 0.5 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004973049665787096 2 0.007130035062995956 3 0.0026363154854774965 4 0.17076134394570147 5 0.0017974878310073842 6 5.392463493022153E-4 7 5.991626103357947E-5 8 7.189951324029537E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.0970691361752814E-4 24-25 7.789113934365331E-4 26-27 0.0027561480075446554 28-29 1.4979065258394867E-4 30-31 0.006650704974727322 32-33 0.021629770233122188 34-35 0.013900572559790436 36-37 0.0011084508291212202 38-39 3.5949756620147683E-4 40-41 0.002456566702376758 42-43 0.011983252206715894 44-45 0.008328360283667546 46-47 0.01132417333534652 48-49 0.010934717638628253 50-51 0.1949675134032676 52-53 0.11399068661638494 54-55 0.01797487831007384 56-57 0.007249867585063115 58-59 0.018663915311960003 60-61 0.12318783268503938 62-63 0.2372983518234915 64-65 0.24355960110150052 66-67 0.25302637034480613 68-69 0.24880227394193877 70-71 0.2355008639924841 72-73 0.1588080498695024 74-75 0.2793595670690643 76-77 0.21764581820447743 78-79 0.006381081800076214 80-81 2.9958130516789735E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.1983252206715894E-4 90-91 0.0056321285371564705 92-93 0.01794492017955705 94-95 0.0027861061380614455 96-97 2.9958130516789735E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1668996.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.50989623211518 #Duplication Level Percentage of deduplicated Percentage of total 1 78.10558677154977 50.38583297781144 2 13.766570511335505 17.76160070316701 3 3.796675917237498 7.347695083438851 4 1.5926244484648178 4.109601516287803 5 0.7855889166978032 2.5339129748637528 6 0.4778634019670226 1.8496151084410903 7 0.3117173191071986 1.407619633655096 8 0.20455111363096662 1.0556456891597807 9 0.1439549878003798 0.8357869192588118 >10 0.7593337892005905 8.641706807218764 >50 0.039700349994850614 1.753061203556354 >100 0.015180682119013324 1.6317149474159878 >500 3.724519397508564E-4 0.15040799326470877 >1k 2.7933895481314225E-4 0.5357984424605441 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3625 0.21719644624672557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3424 0.2051532777789761 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1871 0.1121033243938272 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 2.9958130516789735E-5 0.0 0.0 0.0 0.0 12-13 5.991626103357947E-5 0.0 0.0 0.0 0.0 14-15 5.991626103357947E-5 0.0 0.0 0.0 0.0 16-17 5.991626103357947E-5 0.0 0.0 0.0 0.0 18-19 5.991626103357947E-5 0.0 0.0 0.0 0.0 20-21 5.991626103357947E-5 0.0 0.0 0.0 0.0 22-23 5.991626103357947E-5 0.0 0.0 1.1983252206715894E-4 0.0 24-25 5.991626103357947E-5 0.0 0.0 2.696231746511076E-4 0.0 26-27 5.991626103357947E-5 0.0 0.0 5.092882187854254E-4 0.0 28-29 5.991626103357947E-5 0.0 0.0 0.0011983252206715894 0.0 30-31 5.991626103357947E-5 0.0 0.0 0.0032654362263300814 0.0 32-33 5.991626103357947E-5 0.0 0.0 0.009436811112788766 0.0 34-35 5.991626103357947E-5 0.0 0.0 0.01938291044436296 0.0 36-37 5.991626103357947E-5 0.0 0.0 0.03541051027084546 0.0 38-39 5.991626103357947E-5 0.0 0.0 0.06210320456130512 0.0 40-41 5.991626103357947E-5 0.0 0.0 0.09110267490155757 0.0 42-43 5.991626103357947E-5 0.0 0.0 0.12124055420144805 0.0 44-45 5.991626103357947E-5 0.0 0.0 0.15632152503660884 0.0 46-47 5.991626103357947E-5 0.0 0.0 0.200749432593008 0.0 48-49 5.991626103357947E-5 0.0 0.0 0.24880227394193874 0.0 50-51 5.991626103357947E-5 0.0 0.0 0.3008395466496025 5.991626103357947E-5 52-53 5.991626103357947E-5 0.0 0.0 0.3515886197450443 5.991626103357947E-5 54-55 5.991626103357947E-5 0.0 0.0 0.40922806285934776 5.991626103357947E-5 56-57 5.991626103357947E-5 0.0 0.0 0.47621444269488966 5.991626103357947E-5 58-59 5.991626103357947E-5 0.0 0.0 0.5500013181577428 5.991626103357947E-5 60-61 5.991626103357947E-5 0.0 0.0 0.6273532111520939 5.991626103357947E-5 62-63 5.991626103357947E-5 0.0 0.0 0.7122845111671927 1.1983252206715894E-4 64-65 5.991626103357947E-5 0.0 0.0 0.7975453506179764 1.1983252206715894E-4 66-67 5.991626103357947E-5 0.0 0.0 0.8969464276726846 1.1983252206715894E-4 68-69 5.991626103357947E-5 0.0 0.0 1.0069526829303366 1.1983252206715894E-4 70-71 5.991626103357947E-5 0.0 0.0 1.1296911436576242 1.1983252206715894E-4 72-73 5.991626103357947E-5 0.0 0.0 1.2594997231868739 1.1983252206715894E-4 74-75 5.991626103357947E-5 0.0 0.0 1.3889488051499224 1.1983252206715894E-4 76-77 5.991626103357947E-5 0.0 0.0 1.5335567011544664 1.1983252206715894E-4 78-79 5.991626103357947E-5 0.0 0.0 1.7002437393498846 1.1983252206715894E-4 80-81 5.991626103357947E-5 0.0 0.0 1.884546158289175 1.1983252206715894E-4 82-83 5.991626103357947E-5 0.0 0.0 2.0820900709168866 1.1983252206715894E-4 84-85 5.991626103357947E-5 0.0 0.0 2.2806825181126857 1.1983252206715894E-4 86-87 5.991626103357947E-5 0.0 0.0 2.4846075125404736 1.1983252206715894E-4 88-89 5.991626103357947E-5 0.0 0.0 2.720048460271924 1.1983252206715894E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2335 0.0 55.769733 1 GTATCAA 4155 0.0 37.975365 1 CAACGCA 4990 0.0 30.992567 5 ATCAACG 4995 0.0 30.919369 3 TCAACGC 5050 0.0 30.580788 4 TATCAAC 5290 0.0 29.196884 2 AACGCAG 5315 0.0 29.186697 6 ACGCAGA 6025 0.0 25.511059 7 CGCAGAG 6035 0.0 25.390179 8 GTGGTAT 1180 0.0 22.957666 1 GCAGAGT 6995 0.0 21.295233 9 GTACATG 4375 0.0 20.748667 1 TACATGG 4330 0.0 20.414272 2 GAGTACT 4915 0.0 20.317444 12-13 ACATGGG 4125 0.0 20.160295 3 TATACCG 145 6.200583E-4 19.630157 5 TAACGCA 195 1.02975955E-5 19.494497 4 GCGTGCG 295 3.0668161E-9 19.297441 9 TGGTATC 1195 0.0 19.088955 2 CAGAGTA 6975 0.0 19.077839 10-11 >>END_MODULE