FastQCFastQC Report
Sat 18 Jun 2016
SRR1930996_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930996_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265193
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1012014.467654632519172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.046.0514761
GTATCAA29450.044.903321
ATCAACG34800.036.7779853
TCAACGC35250.036.7287864
CAACGCA36100.035.9915965
TATCAAC38550.033.4990352
AACGCAG38750.033.457046
CGCAGAG44950.028.2375628
ACGCAGA44750.028.0372187
GTACATG33200.024.2384931
GCAGAGT50500.023.6510759
TACATGG33500.022.6398222
ACATGGG33400.021.573623
GTGGTAT9800.020.3977641
AGAGTAC45200.020.2228410-11
GTGTAGC6850.019.4548031
CAGAGTA49400.018.31799110-11
GAGTACT32850.017.54984512-13
GAGTACA26000.016.5596051
AGTACTT37900.016.47902712-13
Produced by FastQC (version 0.11.5)