FastQCFastQC Report
Sat 18 Jun 2016
SRR1930996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265193
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46390.20479491151526602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42350.18695978664952612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.11018928629922484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.052.2836231
GTATCAA51900.042.8599321
CAACGCA59900.036.272445
TCAACGC60550.035.8680534
ATCAACG60750.035.671743
TATCAAC63650.034.3489272
AACGCAG63400.034.270026
ACGCAGA74050.029.1484137
CGCAGAG73800.028.9900368
ACGTGCG1004.999347E-528.462949
GCAGAGT86800.023.9377299
GTACATG51850.021.908981
TACATGG52400.020.8617292
ACATGGG50400.020.7442573
CAGAGTA83200.020.64019610-11
AGAGTAC79700.019.93953510-11
GAGTACT61050.019.89220212-13
GTGGTAT14650.018.168681
GTACTTT68500.017.69411714-15
GAGTACA42100.017.1606791
Produced by FastQC (version 0.11.5)