##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930996_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2265193 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.343295692684904 31.0 31.0 34.0 30.0 34.0 2 31.503912911615036 31.0 31.0 34.0 30.0 34.0 3 31.572092091049196 31.0 31.0 34.0 30.0 34.0 4 35.34414462696998 37.0 35.0 37.0 33.0 37.0 5 35.2163140182757 37.0 35.0 37.0 33.0 37.0 6 35.240514605157266 37.0 35.0 37.0 32.0 37.0 7 35.12056941726378 37.0 35.0 37.0 32.0 37.0 8 35.1261137571942 37.0 35.0 37.0 32.0 37.0 9 36.62918259062252 39.0 35.0 39.0 32.0 39.0 10-11 36.573403679068406 38.5 35.0 39.0 32.0 39.0 12-13 36.46074197651149 38.0 35.0 39.0 32.0 39.0 14-15 37.527510017910174 39.0 36.0 41.0 32.0 41.0 16-17 37.48088705907179 39.0 36.0 41.0 32.0 41.0 18-19 37.49181041085683 39.0 36.0 41.0 32.0 41.0 20-21 37.473569360315 39.0 36.0 41.0 32.0 41.0 22-23 37.37588717605961 39.0 36.0 41.0 32.0 41.0 24-25 37.25501932947877 39.0 36.0 40.5 31.0 41.0 26-27 37.00501612886849 39.0 36.0 40.0 31.0 41.0 28-29 36.82218866118693 39.0 36.0 40.0 30.5 41.0 30-31 36.599833877289925 38.5 35.0 40.0 30.0 41.0 32-33 36.45053357484329 38.0 35.0 40.0 30.0 41.0 34-35 36.54658697956421 38.0 35.0 40.0 30.0 41.0 36-37 36.62915345403239 39.0 35.0 40.0 30.0 41.0 38-39 36.62049480993451 39.0 35.0 40.0 30.0 41.0 40-41 36.513328224129246 39.0 35.0 40.0 30.0 41.0 42-43 36.38818105123934 39.0 35.0 40.0 30.0 41.0 44-45 36.23773934494765 38.5 35.0 40.0 30.0 41.0 46-47 36.0222731572983 38.0 35.0 40.0 29.5 41.0 48-49 35.82672182900089 38.0 35.0 40.0 28.5 41.0 50-51 35.57511854398279 38.0 34.5 40.0 27.5 41.0 52-53 35.3703459263736 38.0 34.0 40.0 27.0 41.0 54-55 35.22201993384228 38.0 34.0 40.0 27.0 41.0 56-57 35.05632058725239 38.0 34.0 40.0 26.5 41.0 58-59 34.856599636322386 38.0 34.0 40.0 26.0 41.0 60-61 34.55507234924353 37.5 33.0 40.0 26.0 41.0 62-63 34.30196786763865 37.0 33.0 40.0 26.0 41.0 64-65 33.992873675664725 37.0 32.5 40.0 25.0 41.0 66-67 33.673017486810174 36.0 32.0 39.0 24.5 41.0 68-69 33.327026880270246 36.0 32.0 39.0 23.5 40.0 70-71 32.94894607214485 35.5 31.5 39.0 23.0 40.0 72-73 32.51789096999681 35.0 31.0 38.0 22.0 40.0 74-75 31.99822597897839 35.0 31.0 37.5 21.0 39.5 76-77 30.608924934873098 33.5 29.5 36.0 20.0 38.5 78-79 31.34791803612319 34.0 30.0 36.0 21.0 39.0 80-81 31.308572823596045 34.0 31.0 36.0 21.0 38.5 82-83 31.030489454982423 34.0 30.5 36.0 20.0 37.0 84-85 30.68832501248238 34.0 30.0 35.0 20.0 37.0 86-87 30.33294160806607 34.0 30.0 35.0 18.5 36.5 88-89 29.979334431988796 34.0 30.0 35.0 17.5 36.0 90-91 29.6367494955176 34.0 29.0 35.0 13.0 36.0 92-93 29.313222317038772 34.0 29.0 35.0 7.0 35.5 94-95 28.92561627199095 34.0 29.0 35.0 2.0 35.0 96-97 28.487368405252887 34.0 29.0 35.0 2.0 35.0 98-99 27.992409476808376 33.5 29.0 35.0 2.0 35.0 100-101 26.847992422720715 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3058881469523129 1101 2 0.3792367255724933 1101 3 0.32770975208229913 1101 4 0.14649755609755744 1101 5 0.31117152706153917 1101 6 0.29632328246896833 1101 7 0.3201156878034723 1101 8 0.39710115719252315 1101 9 0.5481364146880239 1101 10-11 0.4161190098851364 1101 12-13 0.4247303688387447 1101 14-15 0.41902263328451994 1101 16-17 0.5729280252085687 1101 18-19 0.47285404354504124 1101 20-21 0.5303513878672561 1101 22-23 0.4551732838715239 1101 24-25 0.4566300848506728 1101 26-27 0.4489929990735462 1101 28-29 0.6288380232428921 1101 30-31 0.5217298119297595 1101 32-33 0.48588645704125355 1101 34-35 0.3168053379974225 1101 36-37 0.2978908862974734 1101 38-39 0.4094703202862675 1101 40-41 0.6076214754741187 1101 42-43 0.3563020857094088 1101 44-45 0.31023246965633433 1101 46-47 0.45688363462558357 1101 48-49 0.4805715838513791 1101 50-51 0.4411808587904247 1101 52-53 0.28097930269347415 1101 54-55 0.3168467634263976 1101 56-57 0.3869746341282436 1101 58-59 0.33855842037246475 1101 60-61 0.3153562919301294 1101 62-63 0.31599193129156333 1101 64-65 0.2761926046862371 1101 66-67 0.27281781709956476 1101 68-69 0.18361976664078838 1101 70-71 0.18440256888548845 1101 72-73 0.11805858420732562 1101 74-75 0.32750636205057404 1101 76-77 0.24819369465252095 1101 78-79 0.42133751992488655 1101 80-81 0.4996977800201279 1101 82-83 0.3855959046336679 1101 84-85 0.3470730441756693 1101 86-87 0.4156487261835409 1101 88-89 0.48152035682099736 1101 90-91 0.4150201730961349 1101 92-93 0.36840531669447074 1101 94-95 0.3595733096537117 1101 96-97 0.44889761484967394 1101 98-99 0.6017200128471956 1101 100-101 0.7650604427503396 1102 1 0.3162301493659285 1102 2 0.4179940466827574 1102 3 0.4075815426957803 1102 4 0.26044005009120497 1102 5 0.2314414532138187 1102 6 0.3170969674370241 1102 7 0.41386243551441027 1102 8 0.3856984933601879 1102 9 0.5302258512531637 1102 10-11 0.4315423333647246 1102 12-13 0.45843183361437667 1102 14-15 0.6388081759600865 1102 16-17 0.6000662322700379 1102 18-19 0.6271365470602888 1102 20-21 0.5439772334624067 1102 22-23 0.562758452326257 1102 24-25 0.5802480695939778 1102 26-27 0.5065244701600093 1102 28-29 0.54658251234288 1102 30-31 0.6224826822478491 1102 32-33 0.627802512000784 1102 34-35 0.6551131512412951 1102 36-37 0.6505707724811955 1102 38-39 0.46502661394997347 1102 40-41 0.614534596453737 1102 42-43 0.631126877286114 1102 44-45 0.651018665557622 1102 46-47 0.6842726139327198 1102 48-49 0.6745458534010922 1102 50-51 0.682208416058387 1102 52-53 0.6552571561273339 1102 54-55 0.7786529878924711 1102 56-57 0.5876848787003794 1102 58-59 0.7498060814922454 1102 60-61 0.6003592259100472 1102 62-63 0.4691617367631622 1102 64-65 0.599834393417531 1102 66-67 0.44565137740115546 1102 68-69 0.47827881603129896 1102 70-71 0.577549234666435 1102 72-73 0.38063705433055617 1102 74-75 0.423732046000044 1102 76-77 0.4865612946569513 1102 78-79 0.521467611487207 1102 80-81 0.509862082895431 1102 82-83 0.4360155191197741 1102 84-85 0.6446970740247622 1102 86-87 0.5097602838500848 1102 88-89 0.5885253722659094 1102 90-91 0.54102021738262 1102 92-93 0.6426211690433163 1102 94-95 0.684898538559505 1102 96-97 0.6923736613947717 1102 98-99 0.6464172593347186 1102 100-101 0.9552094667868332 1103 1 0.2998238186179947 1103 2 0.37845802591159483 1103 3 0.3595009474160591 1103 4 0.29937022083608866 1103 5 0.35286508715578435 1103 6 0.3384050757115489 1103 7 0.28370217297207034 1103 8 0.3227721900862548 1103 9 0.37047936265435766 1103 10-11 0.45663837873640745 1103 12-13 0.41998949492332116 1103 14-15 0.43165603026873356 1103 16-17 0.513439391171346 1103 18-19 0.515883899902505 1103 20-21 0.5823416876219554 1103 22-23 0.4303609795235346 1103 24-25 0.5283569965173953 1103 26-27 0.5671441084972244 1103 28-29 0.6177849175638173 1103 30-31 0.5397132329226366 1103 32-33 0.583800867079475 1103 34-35 0.5388451067697844 1103 36-37 0.632349090747077 1103 38-39 0.6337389865583702 1103 40-41 0.6214923423544434 1103 42-43 0.6955328034010009 1103 44-45 0.6464289759302275 1103 46-47 0.5398977829879641 1103 48-49 0.4013824439571607 1103 50-51 0.42438056249743994 1103 52-53 0.5210778685014077 1103 54-55 0.3844205481970846 1103 56-57 0.3332326323638952 1103 58-59 0.4338650054468971 1103 60-61 0.2945990325658201 1103 62-63 0.2053272448745176 1103 64-65 0.25376758058451543 1103 66-67 0.19115020125310167 1103 68-69 0.06606366267023844 1103 70-71 0.2606559515017324 1103 72-73 0.29666928276643745 1103 74-75 0.3285564154652043 1103 76-77 0.23433306402490928 1103 78-79 0.3167287867262978 1103 80-81 0.3215847494565409 1103 82-83 0.4049967674567121 1103 84-85 0.4324558614157539 1103 86-87 0.3387232870482997 1103 88-89 0.37657792178398 1103 90-91 0.4019497811779331 1103 92-93 0.20898721974412737 1103 94-95 0.3682363433812341 1103 96-97 0.2971005011007115 1103 98-99 0.33972703305541785 1103 100-101 0.6042865075997916 1104 1 0.36283860390276956 1104 2 0.4613194576552253 1104 3 0.38706521143775774 1104 4 0.2699824045824073 1104 5 0.31450245539246424 1104 6 0.26707845322413704 1104 7 0.32111376380154866 1104 8 0.3749871600785255 1104 9 0.43104887260047775 1104 10-11 0.42457259333871633 1104 12-13 0.5186697627781385 1104 14-15 0.6139781407400235 1104 16-17 0.6551971449776843 1104 18-19 0.6245736202646199 1104 20-21 0.4896407096565767 1104 22-23 0.43225962345785973 1104 24-25 0.4907270064475995 1104 26-27 0.5318814069619506 1104 28-29 0.46675288615775656 1104 30-31 0.45340970610965314 1104 32-33 0.5655220991768957 1104 34-35 0.5182904269825102 1104 36-37 0.5357035366101286 1104 38-39 0.5848130331289738 1104 40-41 0.515407670760311 1104 42-43 0.5900021819095045 1104 44-45 0.6147238116476785 1104 46-47 0.5732556884976319 1104 48-49 0.5511523919321846 1104 50-51 0.40824435085391997 1104 52-53 0.43425611847028733 1104 54-55 0.5261180476976861 1104 56-57 0.6524087370623519 1104 58-59 0.5742246060386478 1104 60-61 0.5869111259849618 1104 62-63 0.5934330087326387 1104 64-65 0.5440377225313497 1104 66-67 0.5888351331168806 1104 68-69 0.5069903775114071 1104 70-71 0.5580377300206578 1104 72-73 0.6307269343756801 1104 74-75 0.6445097290539472 1104 76-77 0.5558055272643543 1104 78-79 0.7446720557594269 1104 80-81 0.6355563658787169 1104 82-83 0.594674788712549 1104 84-85 0.6235521206547467 1104 86-87 0.6449657055005176 1104 88-89 0.7800172303178705 1104 90-91 0.6070836651596245 1104 92-93 0.7408740516632051 1104 94-95 0.8400266526070581 1104 96-97 0.9486270520791109 1104 98-99 0.842142090769272 1104 100-101 1.013972179162078 1105 1 0.31503485609902526 1105 2 0.27112681746258716 1105 3 0.3038246281971766 1105 4 0.09616680576680636 1105 5 0.23315764904766212 1105 6 0.2895876757333582 1105 7 0.26740533509311604 1105 8 0.22793608802744814 1105 9 0.4573911239427346 1105 10-11 0.31235250611863563 1105 12-13 0.3208558649642441 1105 14-15 0.5590330732949624 1105 16-17 0.6418676441481779 1105 18-19 0.40921857990957733 1105 20-21 0.34741990493576935 1105 22-23 0.3869878656861019 1105 24-25 0.45712373534432515 1105 26-27 0.3578638579444018 1105 28-29 0.4353373797422435 1105 30-31 0.323413713613661 1105 32-33 0.393322714477506 1105 34-35 0.4368060579981403 1105 36-37 0.4305631689697549 1105 38-39 0.5139959748119196 1105 40-41 0.4889766068292545 1105 42-43 0.4656656950730138 1105 44-45 0.5289263883502571 1105 46-47 0.3225706503125991 1105 48-49 0.2772861305659191 1105 50-51 0.34783726544682736 1105 52-53 0.09341641513058363 1105 54-55 0.3763264729061149 1105 56-57 0.2985684457220614 1105 58-59 0.39895073076477416 1105 60-61 0.343481770055611 1105 62-63 0.28840420370384123 1105 64-65 0.31060638910001614 1105 66-67 0.4364978307795795 1105 68-69 0.2529967860178104 1105 70-71 0.19013827462119792 1105 72-73 0.2655795317282781 1105 74-75 0.523946858491076 1105 76-77 0.5951327915916167 1105 78-79 0.6052540538414206 1105 80-81 0.5308922612146105 1105 82-83 0.5105961296338926 1105 84-85 0.5155122626148874 1105 86-87 0.5589513694861807 1105 88-89 0.5858309111315521 1105 90-91 0.738352696428656 1105 92-93 0.6483658966550543 1105 94-95 0.5939488440292493 1105 96-97 0.6388896048416655 1105 98-99 0.9618918230190019 1105 100-101 1.0556473333372303 1106 1 0.23211522537145868 1106 2 0.25465539180535757 1106 3 0.26480362550185177 1106 4 0.06574219454888208 1106 5 0.1234427631323598 1106 6 0.10545666822030597 1106 7 0.26099992534742 1106 8 0.19546806516277115 1106 9 0.2747132477366705 1106 10-11 0.30692236102251513 1106 12-13 0.2059981615887878 1106 14-15 0.27032088270384946 1106 16-17 0.31010188127594773 1106 18-19 0.27422233426615605 1106 20-21 0.22378829195134387 1106 22-23 0.17373694011784835 1106 24-25 0.31773727967394194 1106 26-27 0.2596442476162295 1106 28-29 0.32842545738147777 1106 30-31 0.27466567217250315 1106 32-33 0.24947356582669045 1106 34-35 0.3141952827777601 1106 36-37 0.26860413268921945 1106 38-39 0.38767120777733766 1106 40-41 0.25289572106151326 1106 42-43 0.19311850519806484 1106 44-45 0.2741001955282414 1106 46-47 0.3709944715965463 1106 48-49 0.3130223967823511 1106 50-51 0.30433100022867166 1106 52-53 0.18781352622915648 1106 54-55 0.3157771649664056 1106 56-57 0.40887361197712124 1106 58-59 0.3129599061518249 1106 60-61 0.23871438825273827 1106 62-63 0.12393777806830997 1106 64-65 0.3265278991760141 1106 66-67 0.27414860183326795 1106 68-69 0.17276336486580846 1106 70-71 -0.003983227642265774 1106 72-73 0.05908471187228059 1106 74-75 0.36374347219165415 1106 76-77 0.31924313929278014 1106 78-79 0.33207101051224086 1106 80-81 0.3171109513998971 1106 82-83 0.28592848784725433 1106 84-85 0.3913459250873821 1106 86-87 0.18716676972663748 1106 88-89 0.2839428821036485 1106 90-91 0.3109975429774714 1106 92-93 0.36036638276827304 1106 94-95 0.46808762067324494 1106 96-97 0.5111286336568917 1106 98-99 0.5364112436762127 1106 100-101 0.6511091191956773 1107 1 0.1602019083083306 1107 2 0.11213931042205516 1107 3 0.1299678640338655 1107 4 -0.07148843471361488 1107 5 0.01865493015389319 1107 6 0.19244367684950703 1107 7 0.21798812743233498 1107 8 0.1865462053782494 1107 9 0.10385432285000462 1107 10-11 0.19381674711063113 1107 12-13 0.28805792777524886 1107 14-15 0.24483221447935222 1107 16-17 0.1883345463317383 1107 18-19 0.3666271994143102 1107 20-21 0.3003560862895185 1107 22-23 0.34202613000356763 1107 24-25 0.2533115999663238 1107 26-27 0.3038300427258349 1107 28-29 0.18399050677151507 1107 30-31 0.3313368301531767 1107 32-33 0.37170582960462184 1107 34-35 0.18580669013879003 1107 36-37 0.3002411734033501 1107 38-39 0.32660700850828306 1107 40-41 0.3613346636943504 1107 42-43 0.32547326250278985 1107 44-45 0.23813428339079934 1107 46-47 0.15099751577342602 1107 48-49 0.11074531787763675 1107 50-51 0.2503135515602395 1107 52-53 0.1167498977432686 1107 54-55 0.19159740632120048 1107 56-57 0.24208013343424284 1107 58-59 0.31740691239411234 1107 60-61 0.400348419382901 1107 62-63 0.1100518343821264 1107 64-65 0.047404725898353206 1107 66-67 0.11893103831635443 1107 68-69 0.20813064653692237 1107 70-71 0.12324883722637736 1107 72-73 0.23670612004624303 1107 74-75 0.42335381755006196 1107 76-77 0.5079041857631772 1107 78-79 0.5750950024313362 1107 80-81 0.5815573868590249 1107 82-83 0.5066902066310313 1107 84-85 0.5828447152000358 1107 86-87 0.6520672372499376 1107 88-89 0.7706153546152521 1107 90-91 0.6419831990235316 1107 92-93 0.5624847666562722 1107 94-95 0.7132296505511491 1107 96-97 0.7296471745967494 1107 98-99 0.6932395399922413 1107 100-101 0.8558884769089481 1108 1 0.03250789186065006 1108 2 0.22157017512071064 1108 3 0.12594873345886626 1108 4 0.12036119246119625 1108 5 0.08755738952122982 1108 6 0.23191699524388554 1108 7 0.10326271415519273 1108 8 0.09320541646791725 1108 9 0.28835656925381414 1108 10-11 0.20343433717530246 1108 12-13 0.1531013267399075 1108 14-15 0.18534265275051354 1108 16-17 0.1279977178202074 1108 18-19 0.10646538466477296 1108 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-1.6278843041460895 2316 80-81 -1.388067142934041 2316 82-83 -0.9749211062399006 2316 84-85 -0.9519159445280749 2316 86-87 -0.8422464230994322 2316 88-89 -0.841854485560976 2316 90-91 -0.9564950734053994 2316 92-93 -0.9134356618552282 2316 94-95 -1.243610158916482 2316 96-97 -1.156817478248854 2316 98-99 -1.1818890184579445 2316 100-101 -1.5850971159096865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 8.0 5 47.0 6 105.0 7 295.0 8 666.0 9 1327.0 10 2316.0 11 3528.0 12 5086.0 13 6836.0 14 8419.0 15 10716.0 16 12726.0 17 14636.0 18 13094.0 19 10763.0 20 10862.0 21 11661.0 22 12782.0 23 14869.0 24 18330.0 25 23451.0 26 30543.0 27 39388.0 28 50232.0 29 62543.0 30 77212.0 31 93576.0 32 112839.0 33 137390.0 34 169491.0 35 211448.0 36 277811.0 37 382452.0 38 368984.0 39 68729.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.80379700172575 21.974497406961184 15.188404235922162 23.033301355390904 2 14.91507569258862 22.064690905189675 40.8732914587396 22.1469419434821 3 18.263089210947804 26.6628052559921 31.284887345109457 23.789218187950635 4 12.485583335397068 18.37916889255251 39.77159757870791 29.363650193342504 5 12.099975939730127 38.78568480163522 36.55479009070059 12.559549167934062 6 29.189473874737715 40.08356057766635 17.894889728556148 12.832075819039787 7 25.337101667320034 33.54064467824361 23.54767278005573 17.574580874380626 8 23.82786356934107 38.21983241949867 20.692262866527162 17.2600411446331 9 24.596800360940545 16.945664232584154 21.584606697972315 36.87292870850298 10-11 23.387786382882165 27.79092112680906 29.597720812310474 19.2235716779983 12-13 23.98729379792362 25.03256896873688 29.98225758246648 20.997879650873017 14-15 21.027214899569262 27.014651731662596 27.26165055251363 24.69648281625451 16-17 20.59601543886106 30.234244940718074 28.135991061247317 21.033748559173546 18-19 20.569549702828855 29.084916826071773 30.169702978951463 20.17583049214791 20-21 21.051958928003046 28.259159374057752 30.31752702749832 20.371354670440887 22-23 20.838417541356463 28.08197921228474 30.198164917287073 20.88143832907172 24-25 20.773381394214375 28.262292331854056 30.18213764233125 20.782188631600324 26-27 20.801584892749336 28.46412193520261 30.01281379165724 20.721479380390814 28-29 20.7627091520198 28.58252194022705 29.98732776543762 20.66744114231553 30-31 21.056249496422254 28.288873166839036 29.961557012727134 20.69332032401158 32-33 20.927044092085342 28.38826362921239 29.864483363937044 20.820208914765225 34-35 21.029408388318238 28.378260239908442 29.966876598329968 20.625454773443348 36-37 20.999165184438613 28.289232910009886 30.017270219334435 20.69433168621707 38-39 20.909544814823317 28.393404874120826 30.031635343291597 20.665414967764264 40-41 21.006245716348015 28.355817102821995 29.950124770848763 20.687812409981227 42-43 20.95262567888741 28.4263808109694 29.727177881416512 20.893815628726678 44-45 20.91425745830864 28.56081050504248 29.697385107996332 20.827546928652556 46-47 21.08240812506995 28.53624631640434 29.44125905294201 20.940086505583704 48-49 21.11727364321029 28.585566429515634 29.327020294559418 20.970139632714655 50-51 21.13453145778886 28.79824627170182 29.0817541794692 20.98546809104012 52-53 21.196565897639882 28.762971802351277 29.03299301688323 21.00746928312561 54-55 21.12044832769038 28.774375522127617 29.16209667021669 20.943079479965313 56-57 21.110437339254087 28.828271987104788 29.086547111038215 20.97474356260291 58-59 21.143409457896734 28.74253836952639 29.172673576323778 20.941378596253102 60-61 21.112472998603685 28.870067534984127 29.18491254575375 20.832546920658437 62-63 21.13751000920216 28.780015713811274 29.166156849148518 20.91631742783805 64-65 20.90646480716657 28.722959121017233 29.154263422664716 21.216312649151476 66-67 20.95136320322773 28.709390591660426 29.11467141360552 21.22457479150632 68-69 21.011672807337824 28.82211969329837 29.10054104291831 21.06566645644549 70-71 21.04289403237801 28.658710486355982 29.09940608535175 21.19898939591426 72-73 21.078868246488668 28.787890512467502 29.0212975948038 21.11194364624003 74-75 21.055797048675405 28.901646199211644 28.900008123967762 21.14254862814519 76-77 20.95452551464991 28.830770176906796 28.974009618169 21.240694690274296 78-79 20.91394569145691 28.859901159263913 28.966278126211336 21.259875023067842 80-81 21.00303197256568 28.9466632409085 28.842124641090027 21.208180145435797 82-83 21.037721730554527 28.91577009111365 28.939233875435775 21.107274302896045 84-85 21.114558450427843 28.93603326515666 28.71062200880896 21.23878627560654 86-87 20.97814623301414 29.0196023032033 28.791321534191567 21.21092992959099 88-89 21.058135936439797 28.85339470269836 28.728173474113223 21.360295886748624 90-91 21.030157365040626 28.853951255865205 28.897923756275794 21.217967622818374 92-93 21.000232475631453 28.887183527920367 28.867512512951187 21.245071483496993 94-95 20.86750813363374 28.843623427602328 28.8380166890973 21.45085174966663 96-97 20.907732806873412 28.68225797978362 28.919014847741455 21.49099436560152 98-99 20.784476201365624 28.773839580115247 28.78266884985076 21.659015368668367 100-101 20.761895344017045 28.80527178037368 28.778298361331682 21.65453451427759 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 160.0 1 124.0 2 128.5 3 176.5 4 142.5 5 133.5 6 277.0 7 606.0 8 1021.5 9 1288.5 10 1189.5 11 1029.5 12 1082.5 13 1191.0 14 1385.0 15 1629.0 16 1811.5 17 2022.5 18 2318.5 19 2635.0 20 3027.0 21 3485.0 22 4147.0 23 5037.0 24 6375.0 25 8382.5 26 11149.5 27 14834.0 28 19742.0 29 27003.5 30 35851.5 31 45177.5 32 54690.5 33 62697.5 34 69205.5 35 74603.5 36 81073.5 37 87760.0 38 90551.5 39 90917.0 40 90583.5 41 89955.5 42 89982.0 43 90966.0 44 92075.0 45 93172.5 46 93266.0 47 92672.0 48 91314.0 49 88053.5 50 82961.0 51 76211.0 52 69451.0 53 62774.5 54 55995.0 55 47550.5 56 38044.5 57 29630.0 58 22524.0 59 16917.5 60 12216.5 61 8540.5 62 5887.0 63 3654.0 64 2087.5 65 1220.5 66 638.5 67 357.0 68 193.0 69 107.5 70 72.5 71 45.0 72 32.5 73 21.0 74 11.0 75 8.0 76 7.5 77 4.0 78 2.5 79 1.5 80 2.5 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003973171380981665 2 0.007769757367253034 3 0.0019865856904908324 4 0.17309783316476784 5 0.0021190247365235546 6 4.4146348677574055E-4 7 4.414634867757406E-5 8 5.739025328084627E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.973171380981665E-4 24-25 4.635366611145275E-4 26-27 0.0024501223516053597 28-29 1.9865856904908324E-4 30-31 0.0066440254759748945 32-33 0.0216096376776725 34-35 0.014524148714921864 36-37 9.712196709066292E-4 38-39 2.2073174338787027E-4 40-41 0.0017879271214417492 42-43 0.01209609953765529 44-45 0.008189147679689986 46-47 0.011146953041087449 48-49 0.011080733518071088 50-51 0.19466332449376278 52-53 0.11447148212094951 54-55 0.018585612793258675 56-57 0.00735036705481608 58-59 0.019424393418132582 60-61 0.12405123978398308 62-63 0.23827991698720596 64-65 0.24437211310471119 66-67 0.2548127245669574 68-69 0.25022150430448975 70-71 0.23646991669142542 72-73 0.16305453884061977 74-75 0.284501144052626 76-77 0.2203564994241109 78-79 0.005959757071472497 80-81 4.414634867757406E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 8.829269735514812E-5 90-91 0.006224635163537941 92-93 0.019402320243793795 94-95 0.0027150004436708045 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2265193.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.6263712439622 #Duplication Level Percentage of deduplicated Percentage of total 1 74.86827570382121 44.641236015113584 2 14.905234983673335 17.77490149229998 3 4.599758425172207 8.228007104755827 4 1.9929004465680003 4.753176875172864 5 1.0741162410196385 3.202282687310307 6 0.6201653726837477 2.218692644657479 7 0.41066836555023417 1.7140665095714607 8 0.2655521689044209 1.2667129766187482 9 0.20991003454277907 1.1264556282732605 >10 0.9846448675832669 10.228622946970898 >50 0.04647114899341478 1.8531569890797035 >100 0.021411604543271224 2.205045811029992 >500 5.937579629745257E-4 0.23698306658219737 >1k 2.968789814868373E-4 0.550659252563771 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4639 0.20479491151526602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4235 0.18695978664952612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2496 0.11018928629922484 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2073174338787025E-5 0.0 12-13 0.0 0.0 0.0 4.414634867757405E-5 0.0 14-15 0.0 0.0 0.0 4.414634867757405E-5 0.0 16-17 0.0 0.0 0.0 4.414634867757405E-5 4.414634867757405E-5 18-19 0.0 0.0 0.0 4.414634867757405E-5 4.414634867757405E-5 20-21 0.0 0.0 0.0 4.414634867757405E-5 4.414634867757405E-5 22-23 0.0 0.0 0.0 6.621952301636108E-5 4.414634867757405E-5 24-25 0.0 0.0 0.0 1.3243904603272216E-4 4.414634867757405E-5 26-27 0.0 0.0 0.0 3.0902444074301834E-4 4.414634867757405E-5 28-29 0.0 0.0 0.0 7.063415788411848E-4 4.414634867757405E-5 30-31 0.0 0.0 0.0 0.002582561397638082 4.414634867757405E-5 32-33 0.0 0.0 0.0 0.007659391495559098 4.414634867757405E-5 34-35 0.0 0.0 0.0 0.014590368237938224 4.414634867757405E-5 36-37 0.0 0.0 0.0 0.02419219907531058 4.414634867757405E-5 38-39 0.0 0.0 0.0 0.04176244584898506 4.414634867757405E-5 40-41 0.0 0.0 0.0 0.06264366877347757 4.414634867757405E-5 42-43 0.0 0.0 0.0 0.0831937940828883 4.414634867757405E-5 44-45 0.0 0.0 0.0 0.10756257855290918 4.414634867757405E-5 46-47 0.0 0.0 0.0 0.13663294915709168 4.414634867757405E-5 48-49 0.0 0.0 0.0 0.1668952711755687 4.414634867757405E-5 50-51 0.0 0.0 0.0 0.20152808171312556 4.414634867757405E-5 52-53 0.0 0.0 0.0 0.23750735588534838 4.414634867757405E-5 54-55 0.0 0.0 0.0 0.2809252898097425 4.414634867757405E-5 56-57 0.0 0.0 0.0 0.32950834652941274 4.414634867757405E-5 58-59 0.0 0.0 0.0 0.3860156728367075 4.414634867757405E-5 60-61 0.0 0.0 0.0 0.4482399512977482 4.414634867757405E-5 62-63 0.0 0.0 0.0 0.5127819130643614 4.414634867757405E-5 64-65 0.0 0.0 0.0 0.5747413134333366 4.414634867757405E-5 66-67 0.0 0.0 0.0 0.6482670571558362 4.414634867757405E-5 68-69 0.0 0.0 0.0 0.7363390227675963 6.621952301636108E-5 70-71 0.0 0.0 0.0 0.8292891599082286 8.82926973551481E-5 72-73 0.0 0.0 0.0 0.9248439316208377 8.82926973551481E-5 74-75 0.0 0.0 0.0 1.0308834611443705 8.82926973551481E-5 76-77 0.0 0.0 0.0 1.14826860227804 8.82926973551481E-5 78-79 0.0 4.414634867757405E-5 0.0 1.2803103311726638 8.82926973551481E-5 80-81 0.0 4.414634867757405E-5 0.0 1.431312916824306 8.82926973551481E-5 82-83 0.0 4.414634867757405E-5 0.0 1.5938818458294723 8.82926973551481E-5 84-85 0.0 4.414634867757405E-5 0.0 1.758481506873807 8.82926973551481E-5 86-87 0.0 4.414634867757405E-5 0.0 1.9387089753500033 8.82926973551481E-5 88-89 0.0 4.414634867757405E-5 0.0 2.1420029110102314 8.82926973551481E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2800 0.0 52.283623 1 GTATCAA 5190 0.0 42.859932 1 CAACGCA 5990 0.0 36.27244 5 TCAACGC 6055 0.0 35.868053 4 ATCAACG 6075 0.0 35.67174 3 TATCAAC 6365 0.0 34.348927 2 AACGCAG 6340 0.0 34.27002 6 ACGCAGA 7405 0.0 29.148413 7 CGCAGAG 7380 0.0 28.990036 8 ACGTGCG 100 4.999347E-5 28.46294 9 GCAGAGT 8680 0.0 23.937729 9 GTACATG 5185 0.0 21.90898 1 TACATGG 5240 0.0 20.861729 2 ACATGGG 5040 0.0 20.744257 3 CAGAGTA 8320 0.0 20.640196 10-11 AGAGTAC 7970 0.0 19.939535 10-11 GAGTACT 6105 0.0 19.892202 12-13 GTGGTAT 1465 0.0 18.16868 1 GTACTTT 6850 0.0 17.694117 14-15 GAGTACA 4210 0.0 17.160679 1 >>END_MODULE