FastQCFastQC Report
Sat 18 Jun 2016
SRR1930995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604755
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1561674.332250041958469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.050.2847141
GTATCAA42050.040.9776421
CAACGCA48450.035.6814965
ATCAACG47900.034.9464343
TCAACGC49000.034.77464
TATCAAC51150.032.9766432
AACGCAG54000.032.2636876
GTACATG56100.028.2541241
ACGCAGA61900.027.5190227
ACATGGG56500.026.3352493
CGCAGAG62850.026.1506298
TACATGG59200.025.729582
GCAGAGT68950.022.3812189
CATGGGG37400.019.6218434
AGAGTAC62000.018.7352310-11
CAGAGTA67650.017.5437710-11
GAGTACA41000.017.0851861
GTATATG14100.017.0436171
GAGTACT41800.016.82706312-13
CATGGGA23750.016.719454
Produced by FastQC (version 0.11.5)