##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930995_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3604755 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.291513015447652 28.0 2.0 31.0 2.0 33.0 2 20.046043905896518 27.0 2.0 31.0 2.0 33.0 3 20.695500526388063 27.0 2.0 31.0 2.0 33.0 4 22.27359584770671 32.0 2.0 35.0 2.0 37.0 5 21.664660705096463 30.0 2.0 35.0 2.0 37.0 6 22.59538859090285 31.0 2.0 35.0 2.0 37.0 7 22.75790726415526 30.0 2.0 35.0 2.0 37.0 8 23.1632047115546 32.0 2.0 35.0 2.0 37.0 9 24.43703413962946 32.0 2.0 37.0 2.0 39.0 10-11 24.762644895422852 32.0 2.0 37.0 2.0 39.0 12-13 24.650191067076683 32.0 2.0 37.0 2.0 39.0 14-15 24.644889042389842 32.0 2.0 38.0 2.0 40.0 16-17 23.534477793913872 31.5 2.0 38.0 2.0 40.0 18-19 22.496382000995908 29.5 2.0 38.0 2.0 40.0 20-21 22.147349126362265 28.5 2.0 37.5 2.0 40.0 22-23 21.79218601541575 27.0 2.0 37.0 2.0 40.0 24-25 21.624502636101482 27.0 2.0 37.0 2.0 39.5 26-27 21.331438613719932 27.0 2.0 37.0 2.0 39.0 28-29 21.13443687573774 27.0 2.0 37.0 2.0 39.0 30-31 21.101489282905497 27.0 2.0 37.0 2.0 39.0 32-33 20.98308359375325 26.5 2.0 37.0 2.0 39.0 34-35 20.65271883942182 25.0 2.0 36.0 2.0 39.0 36-37 20.227668177171545 25.0 2.0 36.0 2.0 39.0 38-39 20.082524748561276 24.0 2.0 36.0 2.0 39.0 40-41 19.930408169209834 24.0 2.0 35.0 2.0 39.0 42-43 19.784571350896247 24.0 2.0 35.0 2.0 39.0 44-45 19.403100626810975 23.5 2.0 35.0 2.0 38.0 46-47 19.086284227360807 23.0 2.0 35.0 2.0 38.0 48-49 18.834888224026322 21.0 2.0 35.0 2.0 38.0 50-51 16.534230065566177 11.5 2.0 33.0 2.0 37.5 52-53 18.45803723137911 21.0 2.0 34.0 2.0 38.0 54-55 19.239977474197275 23.0 2.0 35.5 2.0 39.0 56-57 19.204371864384683 23.0 2.0 36.0 2.0 39.0 58-59 18.9119246661701 22.0 2.0 35.0 2.0 39.0 60-61 18.609890547346488 20.5 2.0 35.0 2.0 39.0 62-63 18.14995762541421 18.5 2.0 34.0 2.0 38.0 64-65 17.780545141070615 15.5 2.0 34.0 2.0 38.0 66-67 17.401312849278245 13.0 2.0 34.0 2.0 38.0 68-69 16.940056813847264 8.0 2.0 33.5 2.0 37.5 70-71 16.557226635374665 7.5 2.0 33.0 2.0 37.0 72-73 16.141927676083398 4.5 2.0 32.0 2.0 36.0 74-75 15.670286052727578 2.0 2.0 32.0 2.0 36.0 76-77 15.232430914167537 2.0 2.0 31.0 2.0 35.0 78-79 14.835028178059257 2.0 2.0 31.0 2.0 35.0 80-81 14.385890858047219 2.0 2.0 30.5 2.0 34.5 82-83 13.914349380193661 2.0 2.0 30.0 2.0 34.0 84-85 13.50083722749535 2.0 2.0 29.5 2.0 34.0 86-87 13.006075170157196 2.0 2.0 29.0 2.0 34.0 88-89 12.569362550298148 2.0 2.0 29.0 2.0 34.0 90-91 12.064274409772647 2.0 2.0 28.5 2.0 34.0 92-93 11.499754907060257 2.0 2.0 26.5 2.0 34.0 94-95 10.913776525727823 2.0 2.0 25.5 2.0 33.0 96-97 10.2559383370021 2.0 2.0 25.0 2.0 33.0 98-99 9.561918909884305 2.0 2.0 21.0 2.0 32.0 100-101 8.613269833872206 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.065004353479711 1101 2 9.663084108689446 1101 3 8.995919613735886 1101 4 11.25877457900896 1101 5 11.916144263401549 1101 6 11.08178299829876 1101 7 10.822293092210714 1101 8 10.205312060179303 1101 9 10.001858047422996 1101 10-11 9.602787359621718 1101 12-13 9.513818743316232 1101 14-15 10.133143640437325 1101 16-17 11.176661139776389 1101 18-19 12.04385751179997 1101 20-21 12.06072656199348 1101 22-23 12.27862158998072 1101 24-25 12.16560091883888 1101 26-27 12.295600615227034 1101 28-29 12.169667400273415 1101 30-31 11.815803280759269 1101 32-33 11.700687441987807 1101 34-35 11.610072654831448 1101 36-37 12.08836867805962 1101 38-39 11.90165876308269 1101 40-41 11.610813344020794 1101 42-43 11.187327677949174 1101 44-45 11.085599467059374 1101 46-47 11.022021359154447 1101 48-49 11.168550425235956 1101 50-51 12.702240890070225 1101 52-53 12.047893153061342 1101 54-55 11.710564249465037 1101 56-57 11.717632101483243 1101 58-59 11.647306512124612 1101 60-61 11.340204678193096 1101 62-63 11.426078467948013 1101 64-65 11.313588566327002 1101 66-67 10.810367729678749 1101 68-69 10.884091937566026 1101 70-71 10.62883167185121 1101 72-73 10.499850836358661 1101 74-75 10.155111853911217 1101 76-77 10.127078549773193 1101 78-79 9.84893714835927 1101 80-81 9.3406628882019 1101 82-83 9.03118673738163 1101 84-85 8.930091473053906 1101 86-87 8.737995359543925 1101 88-89 8.617871102784806 1101 90-91 8.384166003633089 1101 92-93 7.985398805943966 1101 94-95 7.6928426978940365 1101 96-97 7.519221827195064 1101 98-99 7.202630546252543 1101 100-101 6.557461210050471 1102 1 7.836064132086193 1102 2 8.872253918997448 1102 3 8.487964777437611 1102 4 10.333068593424215 1102 5 11.147918835025187 1102 6 10.395745871583667 1102 7 10.106065226162237 1102 8 9.643792586622219 1102 9 9.523741129494123 1102 10-11 8.65575861616838 1102 12-13 8.851162387662232 1102 14-15 9.314131958070448 1102 16-17 10.198158344373926 1102 18-19 11.157616743893982 1102 20-21 11.255814953791013 1102 22-23 11.568177303945685 1102 24-25 11.307368178755343 1102 26-27 11.581678522547055 1102 28-29 11.339968973828729 1102 30-31 11.220513146065446 1102 32-33 11.171549928871567 1102 34-35 10.995018000311251 1102 36-37 11.046143405474336 1102 38-39 11.184700053608825 1102 40-41 10.706832685679128 1102 42-43 10.42169669463911 1102 44-45 10.379290844939792 1102 46-47 10.18793492652787 1102 48-49 9.809707612539377 1102 50-51 11.712118895185146 1102 52-53 11.059987908073328 1102 54-55 10.75562518144914 1102 56-57 10.427194662400499 1102 58-59 10.488946193551502 1102 60-61 10.28725731397516 1102 62-63 10.340058556801296 1102 64-65 9.933833420148499 1102 66-67 9.699542671928043 1102 68-69 9.579602536268506 1102 70-71 9.228157973639469 1102 72-73 9.105931466749567 1102 74-75 8.888783738269725 1102 76-77 8.500148753290011 1102 78-79 8.161384331584628 1102 80-81 7.814905975954824 1102 82-83 7.852003108798025 1102 84-85 7.595314781231565 1102 86-87 7.438317153209775 1102 88-89 7.391263165425906 1102 90-91 7.1950719680093 1102 92-93 6.737432108577295 1102 94-95 6.5232683675501875 1102 96-97 6.326637547823697 1102 98-99 6.127292797093194 1102 100-101 5.4898046809316146 1103 1 8.18173411574989 1103 2 9.480206315674916 1103 3 8.903504521098782 1103 4 10.947163450701982 1103 5 11.585125114785 1103 6 10.609950149845968 1103 7 10.245756076659575 1103 8 9.880798168743212 1103 9 9.913122441275124 1103 10-11 9.424796839299102 1103 12-13 9.183943030465326 1103 14-15 9.81162601260035 1103 16-17 10.866957930427045 1103 18-19 11.604866230640681 1103 20-21 12.097118628900201 1103 22-23 12.192560352245824 1103 24-25 12.144880507116202 1103 26-27 11.893955396203534 1103 28-29 11.523055654559741 1103 30-31 11.401261840901078 1103 32-33 11.534491856270481 1103 34-35 11.597289905267406 1103 36-37 11.747335886435415 1103 38-39 11.37856503574164 1103 40-41 11.29116127110374 1103 42-43 10.77475707747594 1103 44-45 10.826223197255441 1103 46-47 10.845252424628232 1103 48-49 10.882160342467714 1103 50-51 12.5163664402039 1103 52-53 11.686971714863393 1103 54-55 11.295290537383622 1103 56-57 10.920068423329521 1103 58-59 10.983383462091215 1103 60-61 10.889132960989528 1103 62-63 10.720292920355469 1103 64-65 10.327604699267571 1103 66-67 10.308151786629171 1103 68-69 10.219694767812648 1103 70-71 9.824004330866323 1103 72-73 9.713870344122146 1103 74-75 9.416665063249836 1103 76-77 9.136740303140988 1103 78-79 8.93996284356028 1103 80-81 8.366485681827404 1103 82-83 8.138064769493782 1103 84-85 7.9609926601693175 1103 86-87 7.831972273038135 1103 88-89 7.7420043470857305 1103 90-91 7.364010731748563 1103 92-93 6.898123811715845 1103 94-95 6.583902699028034 1103 96-97 6.404576482831207 1103 98-99 6.053764962231929 1103 100-101 5.324434906952334 1104 1 7.779429483441859 1104 2 9.346184212540333 1104 3 8.69107079168164 1104 4 10.628135511977813 1104 5 11.37562225885452 1104 6 10.65216499725219 1104 7 10.132908036298026 1104 8 9.732266394224354 1104 9 9.679052189163539 1104 10-11 8.987017282172811 1104 12-13 8.750242054296375 1104 14-15 9.472825161557836 1104 16-17 10.320700451177533 1104 18-19 11.181353835427679 1104 20-21 11.460425046415633 1104 22-23 11.729865360306864 1104 24-25 11.381713997047886 1104 26-27 11.454632310755077 1104 28-29 11.276546386193065 1104 30-31 11.090378954187912 1104 32-33 11.12570621111502 1104 34-35 11.055586164995635 1104 36-37 11.190365674999267 1104 38-39 10.606008298766952 1104 40-41 10.367509646598219 1104 42-43 9.985236060841913 1104 44-45 10.154594099661931 1104 46-47 9.979100110550196 1104 48-49 10.124068124236452 1104 50-51 11.7570062112589 1104 52-53 11.039398664118469 1104 54-55 10.779644611037579 1104 56-57 10.547333200214581 1104 58-59 10.496746706194632 1104 60-61 10.282903986377285 1104 62-63 10.117702556600257 1104 64-65 9.93950235438886 1104 66-67 9.763254249562141 1104 68-69 9.530578450319485 1104 70-71 9.386922131192971 1104 72-73 9.278255241978911 1104 74-75 8.921045665204774 1104 76-77 8.429026910376399 1104 78-79 8.070614864301845 1104 80-81 7.8991934936563855 1104 82-83 7.618290023390022 1104 84-85 7.1968382241593645 1104 86-87 6.9277494319883015 1104 88-89 6.528329421741558 1104 90-91 6.446486614702399 1104 92-93 5.951521220971493 1104 94-95 5.485528934532308 1104 96-97 5.07090652877549 1104 98-99 4.788504130822687 1104 100-101 4.104397715016173 1105 1 7.997439170697579 1105 2 9.016629361160085 1105 3 8.473101885220672 1105 4 10.564223123660664 1105 5 10.556399888044847 1105 6 10.3497871135251 1105 7 9.914808712047908 1105 8 9.474534597802084 1105 9 9.366393507457236 1105 10-11 8.914937227453866 1105 12-13 8.413964428336211 1105 14-15 9.170297075299594 1105 16-17 10.153256728674297 1105 18-19 10.636476387772454 1105 20-21 11.020219180118499 1105 22-23 11.56917590646265 1105 24-25 11.295606964016248 1105 26-27 11.622103085864747 1105 28-29 11.335141927923527 1105 30-31 10.959892524098308 1105 32-33 10.893024522580152 1105 34-35 10.95905398612219 1105 36-37 11.074868407716284 1105 38-39 10.87283340487777 1105 40-41 10.650741552846 1105 42-43 10.126582093556998 1105 44-45 10.244828875908613 1105 46-47 10.195724462095182 1105 48-49 10.076383060182337 1105 50-51 11.645855530663777 1105 52-53 10.616833519827136 1105 54-55 10.372128185664007 1105 56-57 10.225840025546805 1105 58-59 10.181368833773103 1105 60-61 9.829027670893826 1105 62-63 9.921068668002082 1105 64-65 9.586537711741677 1105 66-67 9.456243304158338 1105 68-69 9.2500861251843 1105 70-71 9.09290229153821 1105 72-73 8.8926409297773 1105 74-75 8.650511323737897 1105 76-77 8.392413024556168 1105 78-79 8.27887410579521 1105 80-81 7.787292607945977 1105 82-83 7.531953648859206 1105 84-85 7.23067477634909 1105 86-87 7.076539372940941 1105 88-89 6.887889651341469 1105 90-91 6.540594355139502 1105 92-93 6.272945552812494 1105 94-95 5.811160148556375 1105 96-97 5.551622663757499 1105 98-99 5.010354480649227 1105 100-101 4.41753506634797 1106 1 7.932389924871661 1106 2 9.108871871884919 1106 3 8.743306895506073 1106 4 10.599855771797625 1106 5 11.027672401677933 1106 6 10.321493068192627 1106 7 9.50498195796331 1106 8 9.194187533376365 1106 9 8.88018612050345 1106 10-11 8.678385517871877 1106 12-13 8.466699887354931 1106 14-15 8.973470769170646 1106 16-17 10.137060914795871 1106 18-19 11.342938001542002 1106 20-21 11.427979166173817 1106 22-23 11.502593870656025 1106 24-25 11.249424953670125 1106 26-27 11.414487193985583 1106 28-29 11.436124034731922 1106 30-31 11.022569429294698 1106 32-33 11.410687178152859 1106 34-35 11.295119729073413 1106 36-37 11.483426930645116 1106 38-39 11.078170602028326 1106 40-41 10.84140900143763 1106 42-43 10.707426336820884 1106 44-45 10.214669269736735 1106 46-47 10.05949827272233 1106 48-49 10.19892013636889 1106 50-51 11.945035974230422 1106 52-53 11.189418414347163 1106 54-55 10.466246410800874 1106 56-57 10.474769938863925 1106 58-59 10.10521847061477 1106 60-61 10.200545261808582 1106 62-63 10.243150611128229 1106 64-65 10.147264465187103 1106 66-67 9.688116380638906 1106 68-69 9.384177001590086 1106 70-71 8.999371575416387 1106 72-73 8.77280333900103 1106 74-75 8.484613632067736 1106 76-77 8.374958999932984 1106 78-79 8.06388312483093 1106 80-81 7.8477226773285 1106 82-83 7.752830972129784 1106 84-85 7.444244922954695 1106 86-87 7.172541934703959 1106 88-89 6.834981015651389 1106 90-91 6.553616128926693 1106 92-93 6.278701030548405 1106 94-95 6.172313505187667 1106 96-97 5.568808134707494 1106 98-99 5.1607663095546705 1106 100-101 4.4631964148063314 1107 1 7.360973892391666 1107 2 8.278706747695605 1107 3 8.471886118918206 1107 4 9.985299187746655 1107 5 10.824621594807507 1107 6 9.990920575300812 1107 7 9.863743886670399 1107 8 8.451432397381264 1107 9 5.94796181429933 1107 10-11 7.904530595348337 1107 12-13 7.952232019450026 1107 14-15 8.457137118057389 1107 16-17 9.431028961036706 1107 18-19 10.715737273686884 1107 20-21 10.978366874070726 1107 22-23 10.314803742546513 1107 24-25 10.511096787914983 1107 26-27 10.589445606631006 1107 28-29 10.709004853705803 1107 30-31 10.429030439106633 1107 32-33 10.839078457818154 1107 34-35 10.758655878112283 1107 36-37 11.212521461180426 1107 38-39 10.825700543063185 1107 40-41 10.407621564845225 1107 42-43 10.287686708610103 1107 44-45 9.715871509061959 1107 46-47 10.027212492448147 1107 48-49 10.147477661799698 1107 50-51 11.51041536199865 1107 52-53 10.688797959454991 1107 54-55 10.46114636195118 1107 56-57 10.077160045658179 1107 58-59 10.065966891504576 1107 60-61 9.949295847180995 1107 62-63 9.87318242189972 1107 64-65 10.016355991120761 1107 66-67 9.949191912417522 1107 68-69 9.840620576473373 1107 70-71 9.576300374197643 1107 72-73 9.179514245027299 1107 74-75 9.276639683731473 1107 76-77 9.13121075737911 1107 78-79 8.578341059344702 1107 80-81 8.168606459442922 1107 82-83 8.16882743943052 1107 84-85 7.976550835787496 1107 86-87 7.567541804473544 1107 88-89 7.416376186016686 1107 90-91 7.136498616976061 1107 92-93 6.6093740201230595 1107 94-95 6.349126438768561 1107 96-97 5.858356752112336 1107 98-99 5.431234078590769 1107 100-101 5.065454796196459 1108 1 6.717975352484157 1108 2 7.993127435780153 1108 3 7.9235217524115775 1108 4 9.173031816823034 1108 5 10.97141150644105 1108 6 9.537341433126436 1108 7 8.923052694635775 1108 8 5.789388802659094 1108 9 7.047239789517132 1108 10-11 6.1065601518452475 1108 12-13 4.890039010015297 1108 14-15 7.195989113685627 1108 16-17 8.578518522492814 1108 18-19 8.328990253600505 1108 20-21 8.154763134073608 1108 22-23 9.233223735171553 1108 24-25 8.889044309721921 1108 26-27 7.268279965682861 1108 28-29 9.288448198764929 1108 30-31 9.587578511747296 1108 32-33 10.009393471176615 1108 34-35 9.70059259182933 1108 36-37 9.350033845893517 1108 38-39 9.226171680961578 1108 40-41 9.577120430932286 1108 42-43 8.56632542644244 1108 44-45 8.207682566495631 1108 46-47 9.122135332093915 1108 48-49 9.749774726769026 1108 50-51 11.268055971725612 1108 52-53 10.453438526081552 1108 54-55 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-16.618810180214513 2313 58-59 -16.331877051000557 2313 60-61 -16.037870477254323 2313 62-63 -15.587033183849769 2313 64-65 -15.216960230645153 2313 66-67 -14.85278447361944 2313 68-69 -14.409636097037922 2313 70-71 -14.054731453317757 2313 72-73 -13.650054543473729 2313 74-75 -13.174977359389677 2313 76-77 -12.750591090670172 2313 78-79 -12.380719515603964 2313 80-81 -11.950361713041678 2313 82-83 -11.498010342910343 2313 84-85 -11.09018427714885 2313 86-87 -10.61777647068046 2313 88-89 -10.176667961827254 2313 90-91 -9.691530130457204 2313 92-93 -9.136390866940594 2313 94-95 -8.574256518110289 2313 96-97 -7.943876307435918 2313 98-99 -7.2712813218199095 2313 100-101 -6.358056497438039 2314 1 -18.711151364281847 2314 2 -17.47668339604156 2314 3 -18.11897630407974 2314 4 -19.62173366646279 2314 5 -18.944628918539514 2314 6 -19.91821700170124 2314 7 -20.07876124679983 2314 8 -20.52623700713619 2314 9 -21.784841060444 2314 10-11 -22.15309260793708 2314 12-13 -22.074718699235806 2314 14-15 -22.076102101655138 2314 16-17 -20.88416611083188 2314 18-19 -19.82597217189833 2314 20-21 -19.48685386514899 2314 22-23 -19.122567920697843 2314 24-25 -18.94834879730062 2314 26-27 -18.65708930096653 2314 28-29 -18.468613216109393 2314 30-31 -18.444942866848194 2314 32-33 -18.316209016514495 2314 34-35 -18.009824614700857 2314 36-37 -17.575594555246678 2314 38-39 -17.455870298825932 2314 40-41 -17.3111926035326 2314 42-43 -17.165470375578806 2314 44-45 -16.794908778412374 2314 46-47 -16.494604756011814 2314 48-49 -16.23804595216334 2314 50-51 -14.063506609253917 2314 52-53 -15.898984381407434 2314 54-55 -16.65101996248414 2314 56-57 -16.618810180214513 2314 58-59 -16.331877051000557 2314 60-61 -16.037870477254323 2314 62-63 -15.587033183849769 2314 64-65 -15.216960230645153 2314 66-67 -14.85278447361944 2314 68-69 -14.409636097037922 2314 70-71 -14.054731453317757 2314 72-73 -13.650054543473729 2314 74-75 -13.174977359389677 2314 76-77 -12.750591090670172 2314 78-79 -12.380719515603964 2314 80-81 -11.950361713041678 2314 82-83 -11.498010342910343 2314 84-85 -11.09018427714885 2314 86-87 -10.61777647068046 2314 88-89 -10.176667961827254 2314 90-91 -9.691530130457204 2314 92-93 -9.136390866940594 2314 94-95 -8.574256518110289 2314 96-97 -7.943876307435918 2314 98-99 -7.2712813218199095 2314 100-101 -6.358056497438039 2315 1 -18.711151364281847 2315 2 -17.47668339604156 2315 3 -18.11897630407974 2315 4 -19.62173366646279 2315 5 -18.944628918539514 2315 6 -19.91821700170124 2315 7 -20.07876124679983 2315 8 -20.52623700713619 2315 9 -21.784841060444 2315 10-11 -22.15309260793708 2315 12-13 -22.074718699235806 2315 14-15 -22.076102101655138 2315 16-17 -20.88416611083188 2315 18-19 -19.82597217189833 2315 20-21 -19.48685386514899 2315 22-23 -19.122567920697843 2315 24-25 -18.94834879730062 2315 26-27 -18.65708930096653 2315 28-29 -18.468613216109393 2315 30-31 -18.444942866848194 2315 32-33 -18.316209016514495 2315 34-35 -18.009824614700857 2315 36-37 -17.575594555246678 2315 38-39 -17.455870298825932 2315 40-41 -17.3111926035326 2315 42-43 -17.165470375578806 2315 44-45 -16.794908778412374 2315 46-47 -16.494604756011814 2315 48-49 -16.23804595216334 2315 50-51 -14.063506609253917 2315 52-53 -15.898984381407434 2315 54-55 -16.65101996248414 2315 56-57 -16.618810180214513 2315 58-59 -16.331877051000557 2315 60-61 -16.037870477254323 2315 62-63 -15.587033183849769 2315 64-65 -15.216960230645153 2315 66-67 -14.85278447361944 2315 68-69 -14.409636097037922 2315 70-71 -14.054731453317757 2315 72-73 -13.650054543473729 2315 74-75 -13.174977359389677 2315 76-77 -12.750591090670172 2315 78-79 -12.380719515603964 2315 80-81 -11.950361713041678 2315 82-83 -11.498010342910343 2315 84-85 -11.09018427714885 2315 86-87 -10.61777647068046 2315 88-89 -10.176667961827254 2315 90-91 -9.691530130457204 2315 92-93 -9.136390866940594 2315 94-95 -8.574256518110289 2315 96-97 -7.943876307435918 2315 98-99 -7.2712813218199095 2315 100-101 -6.358056497438039 2316 1 -18.711151364281847 2316 2 -17.47668339604156 2316 3 -18.11897630407974 2316 4 -19.62173366646279 2316 5 -18.944628918539514 2316 6 -19.91821700170124 2316 7 -20.07876124679983 2316 8 -20.52623700713619 2316 9 -21.784841060444 2316 10-11 -22.15309260793708 2316 12-13 -22.074718699235806 2316 14-15 -22.076102101655138 2316 16-17 -20.88416611083188 2316 18-19 -19.82597217189833 2316 20-21 -19.48685386514899 2316 22-23 -19.122567920697843 2316 24-25 -18.94834879730062 2316 26-27 -18.65708930096653 2316 28-29 -18.468613216109393 2316 30-31 -18.444942866848194 2316 32-33 -18.316209016514495 2316 34-35 -18.009824614700857 2316 36-37 -17.575594555246678 2316 38-39 -17.455870298825932 2316 40-41 -17.3111926035326 2316 42-43 -17.165470375578806 2316 44-45 -16.794908778412374 2316 46-47 -16.494604756011814 2316 48-49 -16.23804595216334 2316 50-51 -14.063506609253917 2316 52-53 -15.898984381407434 2316 54-55 -16.65101996248414 2316 56-57 -16.618810180214513 2316 58-59 -16.331877051000557 2316 60-61 -16.037870477254323 2316 62-63 -15.587033183849769 2316 64-65 -15.216960230645153 2316 66-67 -14.85278447361944 2316 68-69 -14.409636097037922 2316 70-71 -14.054731453317757 2316 72-73 -13.650054543473729 2316 74-75 -13.174977359389677 2316 76-77 -12.750591090670172 2316 78-79 -12.380719515603964 2316 80-81 -11.950361713041678 2316 82-83 -11.498010342910343 2316 84-85 -11.09018427714885 2316 86-87 -10.61777647068046 2316 88-89 -10.176667961827254 2316 90-91 -9.691530130457204 2316 92-93 -9.136390866940594 2316 94-95 -8.574256518110289 2316 96-97 -7.943876307435918 2316 98-99 -7.2712813218199095 2316 100-101 -6.358056497438039 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 900970.0 3 36214.0 4 132073.0 5 72187.0 6 43505.0 7 43214.0 8 41894.0 9 41404.0 10 44601.0 11 46608.0 12 47387.0 13 48309.0 14 49037.0 15 50795.0 16 52975.0 17 54310.0 18 54608.0 19 57402.0 20 60194.0 21 63989.0 22 67815.0 23 72842.0 24 77291.0 25 83098.0 26 88322.0 27 95407.0 28 102132.0 29 109463.0 30 118465.0 31 128349.0 32 138878.0 33 146699.0 34 149990.0 35 139036.0 36 100571.0 37 40270.0 38 4431.0 39 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.7090226786055 20.792479098792217 13.982536979059923 23.515961243542357 2 14.858116542203417 20.71302574236572 41.05157765298758 23.37728006244328 3 18.328134898493346 26.055203886248695 30.388503727267093 25.228157487990867 4 12.36187542808103 17.144017796542173 39.332017224410784 31.162089550966016 5 12.705436335715842 38.15242479281955 35.93176907089411 13.210369800570493 6 30.151342987694317 38.69630961355449 17.818259475054194 13.334087923696995 7 26.347632798145522 32.9686376115516 23.298047533512847 17.385682056790035 8 24.732874836756366 36.792340356231016 20.788468892403333 17.68631591460928 9 25.486874627453794 16.673970112358212 21.276707040782668 36.562448219405326 10-11 24.025441430605966 27.011903779656315 29.258493114096723 19.704161675641004 12-13 24.6440876302632 24.44998257465978 29.237351760024715 21.668578035052303 14-15 21.752468086676235 26.287536065766453 26.453976950511294 25.50601889704602 16-17 21.602587937166913 29.413068619032718 27.330128728117693 21.65421471568268 18-19 21.579054242232477 28.319415636914773 28.989889776949024 21.111640343903726 20-21 22.00821938164544 27.75315994600181 28.930582989411686 21.308037682941066 22-23 21.879458614474412 27.63035733315342 28.67531665027289 21.81486740209927 24-25 21.794606589438846 27.790732661350987 28.76959530615315 21.645065443057018 26-27 21.58664486726222 28.04294485232578 28.55630587143963 21.81410440897237 28-29 21.68950743192947 28.11172384548631 28.639366798982664 21.55940192360156 30-31 21.874338904167548 27.83814450833702 28.517406924305966 21.77010966318947 32-33 21.71122494615436 28.055631770519877 28.412764848553262 21.820378434772504 34-35 21.870294497449148 27.950853246290208 28.555447288740087 21.62340496752056 36-37 22.025410005694837 27.71667953166375 28.39996571012518 21.85794475251623 38-39 21.93434890085219 27.79071053489812 28.555401681689062 21.71953888256063 40-41 21.999016129041063 27.78685635029009 28.588148813849468 21.625978706819378 42-43 22.052725268325382 27.74699759041996 28.24982887916358 21.950448262091083 44-45 21.876723964625107 27.995374386707788 28.34423328739113 21.783668361275975 46-47 22.132275751648304 27.900407072026827 27.983709566570248 21.983607609754625 48-49 21.971452851237075 27.943825763377482 28.09989100515942 21.98483038022602 50-51 22.188407815175015 28.195691626417357 27.7930450880498 21.822855470357826 52-53 21.73718982683247 28.154693813518694 28.04748421920198 22.060632140446856 54-55 21.620541394875367 28.20150283288016 28.13053125990615 22.04742451233832 56-57 21.69938147523277 28.20112166106546 28.140913396623272 21.9585834670785 58-59 21.6055119931231 28.066097082407904 28.260146265048675 22.068244659420323 60-61 21.471797270710027 28.36129674790358 28.216316656467104 21.950589324919285 62-63 21.582615079232664 28.23893443768753 28.019205950814513 22.159244532265294 64-65 21.215179749330147 28.208744311189214 28.15545363401153 22.420622305469102 66-67 21.28802183459963 28.141824392281478 28.069706488864938 22.500447284253955 68-69 21.407430443091243 28.025947853919398 28.172166990696162 22.394454712293193 70-71 21.388937523799882 27.915260655486087 28.10417261571455 22.59162920499948 72-73 21.330075898813362 28.05964109315497 28.078001714129197 22.53228129390247 74-75 21.279927208320654 28.310824989482107 27.811837069053162 22.597410733144073 76-77 21.291654094205505 28.134698991116007 27.974743895231697 22.59890301944679 78-79 21.229742615325307 28.202012954575554 27.87427255222798 22.69397187787116 80-81 21.323763621160115 28.14234201461552 27.679204522664474 22.854689841559896 82-83 21.45995538496692 27.84852938430721 27.98783355694196 22.70368167378391 84-85 21.46121719021376 27.996273057500055 27.71630672750487 22.82620302478132 86-87 21.23485404541477 28.124136097959575 27.83169079523782 22.809319061387843 88-89 21.355900343022082 27.89981214432783 27.673896461968305 23.070391050681785 90-91 21.2186695826359 28.01917960953924 27.77226736569847 22.989883442126384 92-93 21.2031371401709 27.87580055849041 27.79838276231235 23.122679539026336 94-95 21.06010832540716 27.635237128221668 27.930800330732442 23.37385421563873 96-97 21.086156121959725 27.562542226048297 27.897626351233544 23.453675300758434 98-99 20.880744351363532 27.68587701884284 27.592405539473745 23.84097309031988 100-101 21.201077956852387 27.537051530859237 27.49913492495636 23.762735587332024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 209262.0 1 188816.5 2 124473.5 3 62955.0 4 38465.5 5 29751.0 6 27692.5 7 28461.0 8 29871.5 9 29728.0 10 27548.0 11 23540.5 12 18654.5 13 13998.5 14 10163.5 15 7636.5 16 6548.5 17 6609.0 18 7617.0 19 9122.0 20 10814.5 21 12645.0 22 14379.5 23 16112.5 24 17954.0 25 19773.0 26 21731.0 27 24257.0 28 27359.0 29 31842.0 30 38282.5 31 46768.0 32 57730.5 33 68578.0 34 78015.0 35 87440.5 36 97632.0 37 106484.0 38 109815.5 39 108683.5 40 106396.5 41 104353.0 42 103265.5 43 103779.0 44 105126.5 45 107002.5 46 108332.5 47 108208.0 48 107180.0 49 104055.0 50 99453.5 51 94187.5 52 88615.0 53 82794.0 54 75372.5 55 66096.5 56 56017.0 57 46123.5 58 37681.0 59 30096.5 60 22781.5 61 17011.0 62 12538.5 63 8823.0 64 6023.5 65 3936.0 66 2494.5 67 1603.0 68 996.0 69 619.5 70 396.0 71 256.5 72 169.5 73 117.0 74 79.0 75 57.5 76 44.0 77 34.5 78 21.0 79 12.0 80 8.0 81 6.0 82 3.5 83 1.5 84 1.5 85 2.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.49313892344972 2 28.34952722168358 3 22.141338315641423 4 28.432667407355005 5 31.407183012437738 6 24.267862864466515 7 23.134776149835425 8 19.25867361304721 9 11.434840925388826 10-11 14.936327156769321 12-13 15.106616122316218 14-15 19.163785056127256 16-17 22.220026048927043 18-19 26.091121310602244 20-21 28.12750103682497 22-23 27.631309201318814 24-25 26.2418666455834 26-27 27.658980984838085 28-29 24.701248767253254 30-31 26.434750766695657 32-33 25.53464798578544 34-35 23.672121961131896 36-37 26.5411380246369 38-39 26.63405696087529 40-41 23.786457054640326 42-43 24.596581459766337 44-45 23.414628733436807 46-47 26.536477513728396 48-49 22.02844576122372 50-51 27.68226966881244 52-53 21.22170577473365 54-55 22.332793768231127 56-57 24.413476089221042 58-59 23.709766683172642 60-61 23.23260526720956 62-63 23.099974894271593 64-65 21.681348663085288 66-67 22.163309295638676 68-69 23.105259580748204 70-71 21.649834732180135 72-73 21.508535254129615 74-75 18.96223182990245 76-77 21.071653968161495 78-79 20.197988490202523 80-81 21.22478504086963 82-83 21.996571195545883 84-85 19.07531857227468 86-87 20.574366357769115 88-89 19.422443411549466 90-91 20.55575205527144 92-93 20.540272501182464 94-95 19.661766194928642 96-97 18.536044752001178 98-99 17.92350659060047 100-101 19.62983614697809 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3604755.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.48361609704179 #Duplication Level Percentage of deduplicated Percentage of total 1 84.54236410043936 57.89766806990817 2 9.154027198693079 12.538017688343514 3 2.852215307526682 5.859900544402899 4 1.2752195786347496 3.493265922506143 5 0.6586150719504662 2.255217087159064 6 0.3826797696936476 1.5724376661482478 7 0.2439308352112389 1.1693685970986074 8 0.174341907010117 0.955165138344561 9 0.11875844305111614 0.7319706859975534 >10 0.5640889772334566 6.479492362257912 >50 0.023271886047133313 1.0623304980809678 >100 0.009780010434905784 1.209774462036671 >500 5.821645328004024E-4 0.2530810171403248 >1k 8.316636087030968E-5 0.07503864898814955 >5k 0.0 0.0 >10k+ 4.158318043515484E-5 4.44727161158723 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 156167 4.332250041958469 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.7741136360168722E-5 2 0.0 0.0 0.0 0.0 2.7741136360168722E-5 3 0.0 0.0 0.0 0.0 2.7741136360168722E-5 4 0.0 0.0 0.0 0.0 2.7741136360168722E-5 5 0.0 0.0 0.0 0.0 2.7741136360168722E-5 6 0.0 0.0 0.0 0.0 8.322340908050616E-5 7 0.0 0.0 0.0 2.7741136360168722E-5 8.322340908050616E-5 8 0.0 0.0 0.0 2.7741136360168722E-5 8.322340908050616E-5 9 0.0 0.0 0.0 2.7741136360168722E-5 8.322340908050616E-5 10-11 0.0 0.0 0.0 2.7741136360168722E-5 8.322340908050616E-5 12-13 0.0 0.0 0.0 2.7741136360168722E-5 8.322340908050616E-5 14-15 1.3870568180084361E-5 0.0 0.0 2.7741136360168722E-5 9.709397726059053E-5 16-17 2.7741136360168722E-5 0.0 0.0 2.7741136360168722E-5 1.1096454544067489E-4 18-19 2.7741136360168722E-5 0.0 0.0 2.7741136360168722E-5 1.1096454544067489E-4 20-21 2.7741136360168722E-5 1.3870568180084361E-5 0.0 2.7741136360168722E-5 1.1096454544067489E-4 22-23 2.7741136360168722E-5 2.7741136360168722E-5 0.0 2.7741136360168722E-5 1.1096454544067489E-4 24-25 2.7741136360168722E-5 2.7741136360168722E-5 0.0 1.387056818008436E-4 1.1096454544067489E-4 26-27 2.7741136360168722E-5 4.161170454025308E-5 0.0 2.774113636016872E-4 1.1096454544067489E-4 28-29 2.7741136360168722E-5 5.5482272720337444E-5 0.0 6.241755681037962E-4 1.1096454544067489E-4 30-31 2.7741136360168722E-5 5.5482272720337444E-5 0.0 0.0022886437497139193 1.1096454544067489E-4 32-33 2.7741136360168722E-5 5.5482272720337444E-5 0.0 0.006588519885540071 1.1096454544067489E-4 34-35 2.7741136360168722E-5 6.93528409004218E-5 0.0 0.013149298634719975 1.1096454544067489E-4 36-37 2.7741136360168722E-5 8.322340908050616E-5 0.0 0.025147340110492945 1.1096454544067489E-4 38-39 2.7741136360168722E-5 8.322340908050616E-5 0.0 0.04394195999450726 1.1096454544067489E-4 40-41 2.7741136360168722E-5 8.322340908050616E-5 0.0 0.06476168283281387 1.1096454544067489E-4 42-43 2.7741136360168722E-5 8.322340908050616E-5 0.0 0.08666330998916708 1.387056818008436E-4 44-45 2.7741136360168722E-5 8.322340908050616E-5 0.0 0.1155695740764629 1.387056818008436E-4 46-47 2.7741136360168722E-5 8.322340908050616E-5 0.0 0.15231270918550638 1.387056818008436E-4 48-49 2.7741136360168722E-5 8.322340908050616E-5 0.0 0.19257896861229126 1.387056818008436E-4 50-51 2.7741136360168722E-5 8.322340908050616E-5 0.0 0.23954471247005693 1.387056818008436E-4 52-53 2.7741136360168722E-5 8.322340908050616E-5 0.0 0.2938063751905469 1.387056818008436E-4 54-55 5.5482272720337444E-5 9.709397726059053E-5 0.0 0.3550033220010791 1.387056818008436E-4 56-57 5.5482272720337444E-5 1.1096454544067489E-4 0.0 0.429460531991772 1.5257624998092796E-4 58-59 5.5482272720337444E-5 1.1096454544067489E-4 0.0 0.5104369090271045 1.6644681816101233E-4 60-61 5.5482272720337444E-5 1.1096454544067489E-4 0.0 0.5967395842435894 1.6644681816101233E-4 62-63 5.5482272720337444E-5 1.1096454544067489E-4 0.0 0.6878276054822033 1.6644681816101233E-4 64-65 5.5482272720337444E-5 1.1096454544067489E-4 0.0 0.7868357211516455 1.9418795452118105E-4 66-67 5.5482272720337444E-5 1.1096454544067489E-4 0.0 0.8992844173875895 1.9418795452118105E-4 68-69 5.5482272720337444E-5 1.1096454544067489E-4 0.0 1.02599205771266 1.9418795452118105E-4 70-71 5.5482272720337444E-5 1.1096454544067489E-4 0.0 1.1612717091730227 1.9418795452118105E-4 72-73 5.5482272720337444E-5 1.1096454544067489E-4 0.0 1.3029873042689446 1.9418795452118105E-4 74-75 5.5482272720337444E-5 1.1096454544067489E-4 0.0 1.449502115955176 1.9418795452118105E-4 76-77 5.5482272720337444E-5 1.387056818008436E-4 0.0 1.6145202655936395 1.9418795452118105E-4 78-79 5.5482272720337444E-5 1.387056818008436E-4 0.0 1.7970569428435499 1.9418795452118105E-4 80-81 5.5482272720337444E-5 1.387056818008436E-4 0.0 1.9923822839554977 2.0805852270126541E-4 82-83 5.5482272720337444E-5 1.387056818008436E-4 0.0 2.2004130655204026 2.2192909088134978E-4 84-85 5.5482272720337444E-5 1.387056818008436E-4 0.0 2.413187581402897 2.3579965906143414E-4 86-87 5.5482272720337444E-5 1.387056818008436E-4 0.0 2.6390143019428507 2.496702272415185E-4 88-89 5.5482272720337444E-5 1.387056818008436E-4 2.7741136360168722E-5 2.8834553249804773 2.496702272415185E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2115 0.0 50.284714 1 GTATCAA 4205 0.0 40.977642 1 CAACGCA 4845 0.0 35.681496 5 ATCAACG 4790 0.0 34.946434 3 TCAACGC 4900 0.0 34.7746 4 TATCAAC 5115 0.0 32.976643 2 AACGCAG 5400 0.0 32.263687 6 GTACATG 5610 0.0 28.254124 1 ACGCAGA 6190 0.0 27.519022 7 ACATGGG 5650 0.0 26.335249 3 CGCAGAG 6285 0.0 26.150629 8 TACATGG 5920 0.0 25.72958 2 GCAGAGT 6895 0.0 22.381218 9 CATGGGG 3740 0.0 19.621843 4 AGAGTAC 6200 0.0 18.73523 10-11 CAGAGTA 6765 0.0 17.54377 10-11 GAGTACA 4100 0.0 17.085186 1 GTATATG 1410 0.0 17.043617 1 GAGTACT 4180 0.0 16.827063 12-13 CATGGGA 2375 0.0 16.71945 4 >>END_MODULE