FastQCFastQC Report
Sat 18 Jun 2016
SRR1930995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604755
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47690.13229747930164462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42260.11723404225807302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.054.0388761
GTATCAA71250.038.7650641
CAACGCA84600.031.9622335
ATCAACG86350.031.3229733
TCAACGC89300.030.3397714
TATCAAC90900.029.6521632
AACGCAG93000.029.4825766
ACGCAGA103350.025.7952867
CGCAGAG104900.025.2784758
GTACATG89850.022.8038651
ACATGGG84750.022.3231983
TACATGG94850.020.9997392
GCAGAGT123550.020.7325889
AGAGTAC111100.019.64021510-11
GAGTACT74700.018.95510512-13
GTGGTAT19900.018.3944551
GTTCGCG1300.00762458218.2448887
CAGAGTA119450.018.14815710-11
CTAACGC2653.981022E-717.9377173
CGTTCGC1350.00947589917.5693956
Produced by FastQC (version 0.11.5)