FastQCFastQC Report
Sat 18 Jun 2016
SRR1930994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315736
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1044344.509754134322738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.057.5166551
GTATCAA32850.045.8683621
ATCAACG39050.037.68313
TCAACGC40800.036.6807984
CAACGCA40300.036.0023735
TATCAAC41750.034.735522
AACGCAG44150.033.7986876
ACGCAGA51900.028.446297
CGCAGAG53400.027.147698
GTACATG41700.026.1785071
TACATGG41700.025.6834952
ACATGGG41250.025.655483
GCAGAGT58100.023.5400569
GTCCTAC11950.023.1593911
GTGTAGC8050.021.6463341
CAACGCG1454.1346104E-420.84625
GAGTACA31450.020.5329421
TAGGACG15250.020.486344
GAGTACT37600.020.05341712-13
CATGGGG25350.019.8776264
Produced by FastQC (version 0.11.5)