##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930994_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315736 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.27686014295239 30.0 2.0 31.0 2.0 33.0 2 19.959329560882587 28.0 2.0 31.0 2.0 33.0 3 20.635367762128325 27.0 2.0 31.0 2.0 33.0 4 22.14205721204835 32.0 2.0 35.0 2.0 37.0 5 21.49450628223597 30.0 2.0 35.0 2.0 37.0 6 22.503171345956535 31.0 2.0 35.0 2.0 37.0 7 22.70971734256409 30.0 2.0 35.0 2.0 37.0 8 23.158489568759133 32.0 2.0 35.0 2.0 37.0 9 24.494704491358256 32.0 2.0 37.0 2.0 39.0 10-11 24.872020385743454 32.5 2.0 37.0 2.0 39.0 12-13 24.79344838962645 33.0 2.0 37.0 2.0 39.0 14-15 24.77172462664138 32.5 2.0 38.0 2.0 40.0 16-17 23.59675088179309 32.0 2.0 38.0 2.0 40.0 18-19 22.52016227238338 30.5 2.0 38.0 2.0 40.0 20-21 22.172070348260768 29.5 2.0 38.0 2.0 40.0 22-23 21.79646319787748 27.0 2.0 37.0 2.0 40.0 24-25 21.64070472627277 27.0 2.0 37.0 2.0 40.0 26-27 21.367437177640284 27.0 2.0 37.0 2.0 39.5 28-29 21.17478589960168 27.0 2.0 37.0 2.0 39.0 30-31 21.168126461738296 27.0 2.0 37.0 2.0 39.0 32-33 21.054033577229873 26.5 2.0 37.0 2.0 39.0 34-35 20.741747116251595 25.0 2.0 37.0 2.0 39.0 36-37 20.277645854276997 25.0 2.0 36.0 2.0 39.0 38-39 20.15120268458926 24.5 2.0 36.0 2.0 39.0 40-41 20.02159766916436 24.0 2.0 36.0 2.0 39.0 42-43 19.894080542859808 24.0 2.0 36.0 2.0 39.0 44-45 19.515093041693873 24.0 2.0 35.0 2.0 39.0 46-47 19.17447822204258 23.0 2.0 35.0 2.0 39.0 48-49 18.94574014481789 21.0 2.0 35.0 2.0 39.0 50-51 16.651122796380932 11.5 2.0 33.0 2.0 37.5 52-53 18.57577288602846 21.0 2.0 34.5 2.0 38.0 54-55 19.36228222906238 23.0 2.0 36.0 2.0 39.0 56-57 19.345096116310323 23.0 2.0 36.0 2.0 39.0 58-59 19.06143899822778 22.5 2.0 36.0 2.0 39.0 60-61 18.773017951959982 21.5 2.0 35.5 2.0 39.0 62-63 18.340228765282397 18.5 2.0 35.0 2.0 39.0 64-65 17.99036828895867 17.5 2.0 34.0 2.0 38.5 66-67 17.62942364760059 13.0 2.0 34.0 2.0 38.0 68-69 17.177356356683145 9.5 2.0 34.0 2.0 38.0 70-71 16.835739479802534 8.0 2.0 33.5 2.0 37.5 72-73 16.438525807777744 7.0 2.0 33.0 2.0 37.0 74-75 15.989810366984837 2.0 2.0 32.0 2.0 36.0 76-77 15.575198554584805 2.0 2.0 32.0 2.0 36.0 78-79 15.19978335181558 2.0 2.0 31.0 2.0 35.0 80-81 14.77106911150494 2.0 2.0 31.0 2.0 35.0 82-83 14.318397908915351 2.0 2.0 31.0 2.0 34.5 84-85 13.913480854467004 2.0 2.0 30.0 2.0 34.0 86-87 13.431575749567308 2.0 2.0 29.5 2.0 34.0 88-89 13.002485602849376 2.0 2.0 29.0 2.0 34.0 90-91 12.513177883834771 2.0 2.0 29.0 2.0 34.0 92-93 11.969006613879992 2.0 2.0 29.0 2.0 34.0 94-95 11.404795926651397 2.0 2.0 27.0 2.0 34.0 96-97 10.746842040716214 2.0 2.0 25.5 2.0 34.0 98-99 10.062252778382337 2.0 2.0 24.5 2.0 33.0 100-101 9.111953391923777 2.0 2.0 17.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.821138710592443 1101 2 9.222869132104929 1101 3 8.815685617472056 1101 4 10.785595864745378 1101 5 11.721575901897715 1101 6 10.89387289382968 1101 7 10.454413893911958 1101 8 9.916973075031397 1101 9 9.806810125272513 1101 10-11 9.276955149765943 1101 12-13 9.09179298426871 1101 14-15 9.766064599621739 1101 16-17 10.84718187536972 1101 18-19 11.812214974387974 1101 20-21 11.838693952549338 1101 22-23 11.858449237260071 1101 24-25 11.887043473254739 1101 26-27 11.985605065448897 1101 28-29 11.895733310022894 1101 30-31 11.549954256582911 1101 32-33 11.374887151099731 1101 34-35 11.343488442222949 1101 36-37 11.606995502897892 1101 38-39 11.296861089521474 1101 40-41 11.13698165973059 1101 42-43 10.78665545858841 1101 44-45 10.840312867664263 1101 46-47 10.513969021406108 1101 48-49 10.57993433443265 1101 50-51 12.47232183019273 1101 52-53 11.526844328618665 1101 54-55 11.310314542525706 1101 56-57 11.193585497241106 1101 58-59 11.062140234118388 1101 60-61 10.817216078994413 1101 62-63 10.806660197592688 1101 64-65 10.787207323789517 1101 66-67 10.498489366516175 1101 68-69 10.42986367024568 1101 70-71 10.111371923156394 1101 72-73 10.186417196267566 1101 74-75 10.040023358007769 1101 76-77 9.832610780372248 1101 78-79 9.367735678825666 1101 80-81 9.236820037995772 1101 82-83 9.074121141639365 1101 84-85 8.773494517536305 1101 86-87 8.537850018576862 1101 88-89 8.323913163673588 1101 90-91 7.7852727444691805 1101 92-93 7.6139689954381655 1101 94-95 7.257917994991711 1101 96-97 7.399023672469699 1101 98-99 7.05976968716822 1101 100-101 6.3445222980707285 1102 1 7.898738479042148 1102 2 9.192289052012296 1102 3 8.627422029413832 1102 4 10.585656918879149 1102 5 11.380647270657747 1102 6 10.368000404107214 1102 7 10.224701807203704 1102 8 9.579856057632565 1102 9 9.243924278535111 1102 10-11 8.434926761372704 1102 12-13 8.630406521742021 1102 14-15 9.10734777890546 1102 16-17 10.006473213350077 1102 18-19 11.182814722735362 1102 20-21 11.358634300241246 1102 22-23 11.376471058420204 1102 24-25 11.055520505283784 1102 26-27 11.295296644230167 1102 28-29 11.006201354356879 1102 30-31 11.030497343065061 1102 32-33 11.029373945030105 1102 34-35 10.907865596662688 1102 36-37 11.081775627073316 1102 38-39 10.665626901199001 1102 40-41 10.460869658839684 1102 42-43 10.015240392057724 1102 44-45 10.09902362210443 1102 46-47 10.195190570170876 1102 48-49 9.655256758786987 1102 50-51 11.664588271001797 1102 52-53 10.884519617758556 1102 54-55 10.653202527580962 1102 56-57 10.404352034381215 1102 58-59 10.449822509260123 1102 60-61 10.317600068935423 1102 62-63 10.476739633788362 1102 64-65 10.139813447597412 1102 66-67 9.968895189714289 1102 68-69 9.668007524956767 1102 70-71 9.320341437183636 1102 72-73 9.320591660589589 1102 74-75 8.822925923473292 1102 76-77 8.741696692184247 1102 78-79 8.481484671263457 1102 80-81 8.062078197181618 1102 82-83 8.012784330070533 1102 84-85 7.963260176044807 1102 86-87 7.653307975280729 1102 88-89 7.309340847702792 1102 90-91 6.936106875224295 1102 92-93 6.726550985785137 1102 94-95 6.304987227568645 1102 96-97 6.071461039947806 1102 98-99 5.878795681606103 1102 100-101 5.4943007900442975 1103 1 7.957259011933097 1103 2 9.400593380132563 1103 3 8.744133406982083 1103 4 10.916776756344962 1103 5 11.399261530197968 1103 6 10.536832268368066 1103 7 10.224942948582896 1103 8 9.57333474076965 1103 9 9.635727046586155 1103 10-11 9.055220785861327 1103 12-13 8.91853643359767 1103 14-15 9.42494864967433 1103 16-17 10.386081017371076 1103 18-19 11.316227065744105 1103 20-21 11.611642880184515 1103 22-23 11.811122007148786 1103 24-25 11.74269530128483 1103 26-27 11.727021697559504 1103 28-29 11.280602919500925 1103 30-31 11.136498841011505 1103 32-33 11.19704446330908 1103 34-35 11.259885986933735 1103 36-37 11.356694998705784 1103 38-39 11.057044595516434 1103 40-41 10.803819860869957 1103 42-43 10.542893078474187 1103 44-45 10.578649887965426 1103 46-47 10.479959902167256 1103 48-49 10.364183714648313 1103 50-51 11.966094354659308 1103 52-53 11.190462142813288 1103 54-55 10.758326694392 1103 56-57 10.434466970272254 1103 58-59 10.257867059167463 1103 60-61 10.315430583994509 1103 62-63 10.221746362297814 1103 64-65 9.9257446505569 1103 66-67 9.814853096719112 1103 68-69 9.601732669282857 1103 70-71 9.41298487518073 1103 72-73 9.35868324881531 1103 74-75 9.239488643856042 1103 76-77 8.833158397428283 1103 78-79 8.36368212020466 1103 80-81 7.9227980514468275 1103 82-83 7.684386870279722 1103 84-85 7.5790275498611965 1103 86-87 7.546566953772249 1103 88-89 7.299139587397846 1103 90-91 7.054416383159383 1103 92-93 6.80187336800997 1103 94-95 6.47927425930726 1103 96-97 6.0874296565697605 1103 98-99 5.792728421010668 1103 100-101 5.061652994702982 1104 1 7.788078191974055 1104 2 9.471548071708796 1104 3 8.695504362019882 1104 4 10.609741858835516 1104 5 11.48089961330178 1104 6 10.657527898865432 1104 7 10.267323021393512 1104 8 9.684628349673108 1104 9 9.385991301004388 1104 10-11 8.767334423051178 1104 12-13 8.742366504071288 1104 14-15 9.338122247429428 1104 16-17 10.256647420273808 1104 18-19 11.330417952125952 1104 20-21 11.34479352337959 1104 22-23 11.61097252553061 1104 24-25 11.540608704081965 1104 26-27 11.516688942995 1104 28-29 11.216042786689922 1104 30-31 11.203880428546103 1104 32-33 11.349586602930277 1104 34-35 11.15401907144841 1104 36-37 11.228449291433549 1104 38-39 10.743742116389331 1104 40-41 10.406907907014514 1104 42-43 10.074057961805817 1104 44-45 10.175160916021579 1104 46-47 9.951886354095109 1104 48-49 10.015943131470927 1104 50-51 11.678825739567113 1104 52-53 10.763597604146277 1104 54-55 10.401272148748607 1104 56-57 10.120594435593734 1104 58-59 10.027444217476024 1104 60-61 9.785457658603754 1104 62-63 9.982648566524155 1104 64-65 9.850916074033911 1104 66-67 9.669836711733403 1104 68-69 9.422380521058027 1104 70-71 9.119529175231104 1104 72-73 8.8445836786507 1104 74-75 8.563427601345097 1104 76-77 8.136636549915115 1104 78-79 8.03206411801307 1104 80-81 7.765168083801333 1104 82-83 7.3785758697482695 1104 84-85 7.032236341146076 1104 86-87 6.94432455638831 1104 88-89 6.7331746562874955 1104 90-91 6.2750589550395315 1104 92-93 6.024220480426726 1104 94-95 5.577971856349134 1104 96-97 5.266210797130954 1104 98-99 5.070032575855276 1104 100-101 4.418063306597633 1105 1 7.894919699102374 1105 2 9.230271601657723 1105 3 8.621056192874118 1105 4 10.732467734819114 1105 5 10.770495452882393 1105 6 10.504727681335169 1105 7 10.172418300568413 1105 8 9.75478815245383 1105 9 9.538966671891622 1105 10-11 8.708821468249756 1105 12-13 8.61130230798523 1105 14-15 9.371722868186534 1105 16-17 10.422202203227343 1105 18-19 10.993159568104428 1105 20-21 11.321436908253926 1105 22-23 11.69613310799885 1105 24-25 11.61075847380631 1105 26-27 11.914671550436704 1105 28-29 11.441290088775563 1105 30-31 11.255967805923877 1105 32-33 10.968434107445578 1105 34-35 10.845676210962822 1105 36-37 11.217860080642268 1105 38-39 11.00553312578452 1105 40-41 10.672196711943158 1105 42-43 10.481423432549171 1105 44-45 10.306839254193736 1105 46-47 10.169647805766132 1105 48-49 10.076922262543626 1105 50-51 11.858307680150677 1105 52-53 10.82512353679114 1105 54-55 10.45761524254527 1105 56-57 10.452466598810812 1105 58-59 10.345008732154135 1105 60-61 10.28380347969123 1105 62-63 10.302972511807607 1105 64-65 10.01762924986867 1105 66-67 9.628880925372208 1105 68-69 9.26610452970278 1105 70-71 9.084809868688751 1105 72-73 8.96788569014246 1105 74-75 8.836462350920858 1105 76-77 8.720354327861811 1105 78-79 8.456626502452243 1105 80-81 7.990331805425852 1105 82-83 7.8574357948472535 1105 84-85 7.561193187270115 1105 86-87 7.244826158191266 1105 88-89 6.883530124073644 1105 90-91 6.646584307807741 1105 92-93 6.443624258399611 1105 94-95 6.135471302405389 1105 96-97 5.771243310185405 1105 98-99 5.403839503459633 1105 100-101 4.9101189965224545 1106 1 7.64032143533025 1106 2 9.05403575365862 1106 3 8.555108429170495 1106 4 10.579346385692833 1106 5 10.956001312962854 1106 6 10.229137749962256 1106 7 9.418070880622963 1106 8 9.051863966077114 1106 9 8.925005606241278 1106 10-11 8.470996112347951 1106 12-13 8.284361631180193 1106 14-15 8.938842398881892 1106 16-17 10.129882209205746 1106 18-19 11.403880588418541 1106 20-21 11.418027225732036 1106 22-23 11.560299556702905 1106 24-25 11.298869173902215 1106 26-27 11.643126257806333 1106 28-29 11.324036417662459 1106 30-31 10.97823038236648 1106 32-33 11.171380712833614 1106 34-35 11.102567192292764 1106 36-37 11.376194597201199 1106 38-39 10.825216270424509 1106 40-41 10.89271508930494 1106 42-43 10.554814806211812 1106 44-45 10.245379527456574 1106 46-47 10.134466896799772 1106 48-49 10.306094876475793 1106 50-51 11.825452369958743 1106 52-53 11.020066555137362 1106 54-55 10.341999404759267 1106 56-57 10.155311820447647 1106 58-59 10.061934089301438 1106 60-61 10.017214532263814 1106 62-63 9.901163546694079 1106 64-65 9.675147465315366 1106 66-67 9.467679266365366 1106 68-69 9.295417636105899 1106 70-71 8.842120583730665 1106 72-73 8.83422105364182 1106 74-75 8.644084814770203 1106 76-77 8.312665603379001 1106 78-79 8.043319075531992 1106 80-81 7.9214244831276535 1106 82-83 7.7681677755669 1106 84-85 7.540852916498276 1106 86-87 7.596672612873725 1106 88-89 7.0071206532868615 1106 90-91 6.8212313952045225 1106 92-93 6.627875650246564 1106 94-95 6.540193493490094 1106 96-97 6.10000266700888 1106 98-99 5.629043454187627 1106 100-101 5.111622564881758 1107 1 7.207454534590923 1107 2 8.185477747272596 1107 3 8.058242963859332 1107 4 9.663423817255126 1107 5 10.728255302188987 1107 6 9.49852063819052 1107 7 9.467913676771776 1107 8 7.973635414921635 1107 9 5.287468507355776 1107 10-11 7.588849331662612 1107 12-13 7.3987867870587145 1107 14-15 7.867669548048159 1107 16-17 9.10110864392027 1107 18-19 10.409470765522645 1107 20-21 10.444066871279151 1107 22-23 10.092798873651152 1107 24-25 10.329276028071686 1107 26-27 10.34605665177427 1107 28-29 10.336642331463363 1107 30-31 10.31933549893385 1107 32-33 10.62465116462647 1107 34-35 10.69015424570945 1107 36-37 10.753181502997148 1107 38-39 10.460542973058438 1107 40-41 9.847432224320823 1107 42-43 9.863955302821022 1107 44-45 9.401654754494817 1107 46-47 9.5627769655943 1107 48-49 9.851725669672192 1107 50-51 11.128229597217196 1107 52-53 10.287254135421435 1107 54-55 9.910279133822204 1107 56-57 9.669259614904124 1107 58-59 9.787538783803992 1107 60-61 9.762257289264582 1107 62-63 9.753008112660567 1107 64-65 9.835695782561302 1107 66-67 9.663611295579592 1107 68-69 9.466406328031209 1107 70-71 9.122346989899743 1107 72-73 8.88242248662323 1107 74-75 8.934431152593326 1107 76-77 8.688592575971297 1107 78-79 8.31621876275546 1107 80-81 8.106543741924748 1107 82-83 8.002616338711467 1107 84-85 8.058808817861527 1107 86-87 7.3991899134531955 1107 88-89 7.311265370544216 1107 90-91 7.116431356056479 1107 92-93 6.806986011020227 1107 94-95 6.5179640125686475 1107 96-97 5.966217010687899 1107 98-99 5.658878339169867 1107 100-101 5.170089185060126 1108 1 6.7269100990687285 1108 2 8.084693119029748 1108 3 7.762341293748062 1108 4 9.13291569633888 1108 5 10.983512970340527 1108 6 9.438001540117153 1108 7 8.653652027044547 1108 8 6.121954395339284 1108 9 6.872255449374194 1108 10-11 6.009349930677409 1108 12-13 4.836904600458848 1108 14-15 7.453365707485592 1108 16-17 8.600703454545865 1108 18-19 8.4304412006308 1108 20-21 8.527040131894761 1108 22-23 9.318925277983926 1108 24-25 8.988919248337186 1108 26-27 7.550981915479284 1108 28-29 9.647280798129735 1108 30-31 9.667631826774016 1108 32-33 10.277143825076628 1108 34-35 10.0350808797151 1108 36-37 9.752045431980168 1108 38-39 9.170029321766183 1108 40-41 9.323894114637433 1108 42-43 8.806562244049385 1108 44-45 8.22789579857211 1108 46-47 9.014067285584083 1108 48-49 9.649714507622832 1108 50-51 11.110521329960712 1108 52-53 10.170719491320142 1108 54-55 9.551795046930064 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6.434664390827471 1109 18-19 5.621885434284003 1109 20-21 7.9134685923970665 1109 22-23 9.111351895324127 1109 24-25 8.536769408009906 1109 26-27 9.052848684668138 1109 28-29 9.385609768676648 1109 30-31 6.877354852349942 1109 32-33 6.138609974657687 1109 34-35 7.615194437189054 1109 36-37 6.384890715597475 1109 38-39 6.427945262124329 1109 40-41 7.458590210042296 1109 42-43 6.05619505931006 1109 44-45 6.584387598441953 1109 46-47 6.336154845983668 1109 48-49 7.18389221779826 1109 50-51 8.910469723395867 1109 52-53 9.250563829338041 1109 54-55 9.228769619057847 1109 56-57 8.051211170512314 1109 58-59 7.913583238819307 1109 60-61 8.03427361580588 1109 62-63 8.80146076523279 1109 64-65 8.751272410062676 1109 66-67 8.370988534083061 1109 68-69 7.992181629360978 1109 70-71 7.386722355090942 1109 72-73 6.978413791392093 1109 74-75 6.079191145500523 1109 76-77 6.029096361527754 1109 78-79 6.660456904387809 1109 80-81 6.684333148386402 1109 82-83 6.333685677438124 1109 84-85 6.093053610635762 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50-51 -14.434951670445297 2313 52-53 -16.318115263044454 2313 54-55 -17.093556150796285 2313 56-57 -17.070344745208917 2313 58-59 -16.77665176928442 2313 60-61 -16.49733092228164 2313 62-63 -16.08934150381097 2313 64-65 -15.735761625593724 2313 66-67 -15.37050255181645 2313 68-69 -14.935087414399153 2313 70-71 -14.597475375865297 2313 72-73 -14.218304198883434 2313 74-75 -13.761975791290403 2313 76-77 -13.35177884106544 2313 78-79 -12.970418298205383 2313 80-81 -12.558373496670326 2313 82-83 -12.117286769887643 2313 84-85 -11.720763245117885 2313 86-87 -11.235650619449508 2313 88-89 -10.809647023480814 2313 90-91 -10.323617089643962 2313 92-93 -9.793942531571618 2313 94-95 -9.241018627722028 2313 96-97 -8.593532686526473 2313 98-99 -7.920451495307322 2313 100-101 -6.977468344209152 2314 1 -18.965335130326316 2314 2 -17.65505515543229 2314 3 -18.312770358708292 2314 4 -19.790754624408176 2314 5 -19.10743303047149 2314 6 -20.140155179330677 2314 7 -20.351626089074006 2314 8 -20.834196639983492 2314 9 -22.164691363455688 2314 10-11 -22.567791766439928 2314 12-13 -22.500082813264253 2314 14-15 -22.458733358693866 2314 16-17 -21.256178396854864 2314 18-19 -20.172685068147114 2314 20-21 -19.83187176416695 2314 22-23 -19.439296402893053 2314 24-25 -19.292241937208896 2314 26-27 -19.02758081290074 2314 28-29 -18.8135393399133 2314 30-31 -18.813082748946652 2314 32-33 -18.70316474171184 2314 34-35 -18.42086715114158 2314 36-37 -17.975522574515974 2314 38-39 -17.82266251745428 2314 40-41 -17.690113193523345 2314 42-43 -17.574680143283135 2314 44-45 -17.20775178567474 2314 46-47 -16.903512901180026 2314 48-49 -16.680167749786833 2314 50-51 -14.434951670445297 2314 52-53 -16.318115263044454 2314 54-55 -17.093556150796285 2314 56-57 -17.070344745208917 2314 58-59 -16.77665176928442 2314 60-61 -16.49733092228164 2314 62-63 -16.08934150381097 2314 64-65 -15.735761625593724 2314 66-67 -15.37050255181645 2314 68-69 -14.935087414399153 2314 70-71 -14.597475375865297 2314 72-73 -14.218304198883434 2314 74-75 -13.761975791290403 2314 76-77 -13.35177884106544 2314 78-79 -12.970418298205383 2314 80-81 -12.558373496670326 2314 82-83 -12.117286769887643 2314 84-85 -11.720763245117885 2314 86-87 -11.235650619449508 2314 88-89 -10.809647023480814 2314 90-91 -10.323617089643962 2314 92-93 -9.793942531571618 2314 94-95 -9.241018627722028 2314 96-97 -8.593532686526473 2314 98-99 -7.920451495307322 2314 100-101 -6.977468344209152 2315 1 -18.965335130326316 2315 2 -17.65505515543229 2315 3 -18.312770358708292 2315 4 -19.790754624408176 2315 5 -19.10743303047149 2315 6 -20.140155179330677 2315 7 -20.351626089074006 2315 8 -20.834196639983492 2315 9 -22.164691363455688 2315 10-11 -22.567791766439928 2315 12-13 -22.500082813264253 2315 14-15 -22.458733358693866 2315 16-17 -21.256178396854864 2315 18-19 -20.172685068147114 2315 20-21 -19.83187176416695 2315 22-23 -19.439296402893053 2315 24-25 -19.292241937208896 2315 26-27 -19.02758081290074 2315 28-29 -18.8135393399133 2315 30-31 -18.813082748946652 2315 32-33 -18.70316474171184 2315 34-35 -18.42086715114158 2315 36-37 -17.975522574515974 2315 38-39 -17.82266251745428 2315 40-41 -17.690113193523345 2315 42-43 -17.574680143283135 2315 44-45 -17.20775178567474 2315 46-47 -16.903512901180026 2315 48-49 -16.680167749786833 2315 50-51 -14.434951670445297 2315 52-53 -16.318115263044454 2315 54-55 -17.093556150796285 2315 56-57 -17.070344745208917 2315 58-59 -16.77665176928442 2315 60-61 -16.49733092228164 2315 62-63 -16.08934150381097 2315 64-65 -15.735761625593724 2315 66-67 -15.37050255181645 2315 68-69 -14.935087414399153 2315 70-71 -14.597475375865297 2315 72-73 -14.218304198883434 2315 74-75 -13.761975791290403 2315 76-77 -13.35177884106544 2315 78-79 -12.970418298205383 2315 80-81 -12.558373496670326 2315 82-83 -12.117286769887643 2315 84-85 -11.720763245117885 2315 86-87 -11.235650619449508 2315 88-89 -10.809647023480814 2315 90-91 -10.323617089643962 2315 92-93 -9.793942531571618 2315 94-95 -9.241018627722028 2315 96-97 -8.593532686526473 2315 98-99 -7.920451495307322 2315 100-101 -6.977468344209152 2316 1 -18.965335130326316 2316 2 -17.65505515543229 2316 3 -18.312770358708292 2316 4 -19.790754624408176 2316 5 -19.10743303047149 2316 6 -20.140155179330677 2316 7 -20.351626089074006 2316 8 -20.834196639983492 2316 9 -22.164691363455688 2316 10-11 -22.567791766439928 2316 12-13 -22.500082813264253 2316 14-15 -22.458733358693866 2316 16-17 -21.256178396854864 2316 18-19 -20.172685068147114 2316 20-21 -19.83187176416695 2316 22-23 -19.439296402893053 2316 24-25 -19.292241937208896 2316 26-27 -19.02758081290074 2316 28-29 -18.8135393399133 2316 30-31 -18.813082748946652 2316 32-33 -18.70316474171184 2316 34-35 -18.42086715114158 2316 36-37 -17.975522574515974 2316 38-39 -17.82266251745428 2316 40-41 -17.690113193523345 2316 42-43 -17.574680143283135 2316 44-45 -17.20775178567474 2316 46-47 -16.903512901180026 2316 48-49 -16.680167749786833 2316 50-51 -14.434951670445297 2316 52-53 -16.318115263044454 2316 54-55 -17.093556150796285 2316 56-57 -17.070344745208917 2316 58-59 -16.77665176928442 2316 60-61 -16.49733092228164 2316 62-63 -16.08934150381097 2316 64-65 -15.735761625593724 2316 66-67 -15.37050255181645 2316 68-69 -14.935087414399153 2316 70-71 -14.597475375865297 2316 72-73 -14.218304198883434 2316 74-75 -13.761975791290403 2316 76-77 -13.35177884106544 2316 78-79 -12.970418298205383 2316 80-81 -12.558373496670326 2316 82-83 -12.117286769887643 2316 84-85 -11.720763245117885 2316 86-87 -11.235650619449508 2316 88-89 -10.809647023480814 2316 90-91 -10.323617089643962 2316 92-93 -9.793942531571618 2316 94-95 -9.241018627722028 2316 96-97 -8.593532686526473 2316 98-99 -7.920451495307322 2316 100-101 -6.977468344209152 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 585491.0 3 23042.0 4 90236.0 5 47377.0 6 26369.0 7 26349.0 8 25890.0 9 25034.0 10 26427.0 11 27778.0 12 29384.0 13 29926.0 14 30705.0 15 31952.0 16 33142.0 17 33538.0 18 32638.0 19 33895.0 20 35550.0 21 38156.0 22 40706.0 23 43746.0 24 46397.0 25 49859.0 26 53713.0 27 58041.0 28 63310.0 29 67940.0 30 73719.0 31 80830.0 32 88612.0 33 95685.0 34 100730.0 35 98602.0 36 78334.0 37 37297.0 38 5285.0 39 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.08523685054442 21.40683626630326 14.405724140575805 23.10220274257652 2 14.33715791211008 20.956103942148406 42.63296880635243 22.073769339389084 3 18.369281194467902 26.61933920207378 30.552282971495092 24.45909663196323 4 11.837894832233422 17.477261900534057 40.53866058602882 30.146182681203705 5 12.137051318942385 38.71072348448678 36.26811178566233 12.884113410908505 6 28.932962574056127 39.55507732201274 18.36753543408449 13.144424669846638 7 25.6161367119759 33.41688308214341 23.51435968622175 17.45262051965895 8 23.887068815008483 37.87325403084354 20.868172538319296 17.371504615828677 9 25.106466014714517 17.025786201141585 22.0542768302692 35.8134709538747 10-11 23.596148928553163 27.520866363217923 29.718150667416765 19.164834040812153 12-13 24.387570137328645 25.043243140862327 29.76057198172091 20.808614740088117 14-15 21.321106183385172 27.335983051709896 27.202904836557174 24.14000592834776 16-17 20.52418394224891 30.131404176705384 28.412901398827717 20.931510482217988 18-19 20.530418249260666 28.7167662667552 30.534753067928655 20.21806241605548 20-21 21.456994557631866 28.21810091328225 30.09556087313308 20.229343655952803 22-23 20.859854839864084 28.34052704556786 29.916016745700453 20.883601368867602 24-25 20.854875488226128 28.30241085974141 30.438634119446224 20.404079532586238 26-27 20.51742803178167 28.71124578422506 30.133547021498934 20.637779162494333 28-29 20.61331498811717 28.685035715334095 30.058456484897313 20.64319281165142 30-31 21.047923463723478 28.308418000127666 30.069791567861397 20.57386696828746 32-33 20.640732494315998 28.698587684548073 30.043686440141986 20.616993380993943 34-35 20.825787405240764 28.61871481849805 30.161307374481165 20.394190401780023 36-37 20.770508118996673 28.37865897827257 30.01907579361149 20.831757109119266 38-39 20.898604701212367 28.785779191276696 29.87173892249934 20.443877185011598 40-41 21.333360884839582 28.317552921134098 30.026549894199345 20.32253629982697 42-43 20.806710401540496 28.703348290373704 29.85210009273868 20.63784121534712 44-45 20.73808251950673 28.998964739507 29.5487257278783 20.714227013107973 46-47 21.112318573473917 28.491094210617586 29.361302817731648 21.03528439817685 48-49 21.01583939064896 28.756851734921973 29.20128707847156 21.0260217959575 50-51 21.13515607821408 29.1580907605847 28.822817571158215 20.883935590043006 52-53 20.64516487099616 29.308145383403982 29.014492552534637 21.03219719306522 54-55 20.693527928788516 29.075628406190223 29.21325595175066 21.017587713270597 56-57 20.79824409938824 29.13364406160949 29.003475413103665 21.064636425898605 58-59 20.731585895663905 29.13072957449928 29.044248138023683 21.09343639181313 60-61 20.601424809921255 29.093220075555248 29.364653181325473 20.940701933198024 62-63 20.65571596889661 29.002211061796203 29.228011942180633 21.11406102712655 64-65 20.548467825008952 29.06741045276602 29.088844352767385 21.29527736945764 66-67 20.545412253318858 29.31701827367588 28.6801308890522 21.457438583953056 68-69 20.690807488634178 28.899419440709284 29.103167401237172 21.306605669419362 70-71 20.9020850209942 28.596032658696863 29.122373562517563 21.379508757791378 72-73 20.782323480791806 29.06164528956176 28.850539298085113 21.30549193156132 74-75 20.403921474720736 29.473212933214555 28.758243617998186 21.364621974066523 76-77 20.561445489492282 29.042645706625898 29.006752190723077 21.38915661315874 78-79 20.429961175274318 29.12341892007726 28.804813532047717 21.6418063726007 80-81 20.466856102650624 29.147760067486566 28.713509523598102 21.67187430626471 82-83 20.540817357338483 29.127334665289812 28.947352175279484 21.384495802092218 84-85 20.689987378674573 28.93287057838701 28.798561541307517 21.578580501630896 86-87 20.281602222961055 29.22458380030341 28.642504221996656 21.851309754738875 88-89 20.629582511657176 29.020444478200602 28.704812332330594 21.645160677811624 90-91 20.54886290697767 28.964817672517317 28.947072302889527 21.539247117615485 92-93 20.456894249128073 29.175724769738732 28.68388455839048 21.683496422742714 94-95 20.206048159802833 29.115051800135568 28.73123233715667 21.947667702904926 96-97 20.427084120858822 28.937560421636455 28.650566026405095 21.98478943109963 98-99 20.334921990798026 28.93512050645442 28.512516332303502 22.21744117044405 100-101 20.37780105690624 29.14772671703797 28.411934803468252 22.062537422587543 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 140519.0 1 127507.0 2 84457.0 3 42471.0 4 26403.5 5 21086.5 6 19748.5 7 19853.5 8 20251.5 9 19823.0 10 18023.5 11 15212.0 12 11898.0 13 8846.5 14 6781.0 15 5622.0 16 5317.5 17 5815.5 18 6734.0 19 7887.0 20 9238.0 21 10678.5 22 12103.5 23 13713.5 24 15424.0 25 17371.0 26 20155.0 27 23380.0 28 26714.0 29 30674.0 30 34948.0 31 39517.0 32 45393.5 33 51995.0 34 58361.0 35 64165.5 36 69943.5 37 73928.0 38 74023.0 39 72467.0 40 71022.5 41 69372.5 42 67208.0 43 65753.0 44 65169.5 45 64654.0 46 63010.0 47 60576.5 48 58149.0 49 55367.5 50 52244.5 51 48457.0 52 44300.5 53 39945.0 54 35583.5 55 31066.5 56 26151.0 57 21366.0 58 17318.0 59 13944.5 60 10934.5 61 8140.5 62 5785.0 63 4008.0 64 2681.5 65 1783.5 66 1177.0 67 779.5 68 515.5 69 332.5 70 230.0 71 153.5 72 100.5 73 73.0 74 52.0 75 37.0 76 26.5 77 22.0 78 14.5 79 12.5 80 9.5 81 6.0 82 4.5 83 1.5 84 1.5 85 1.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.3030172696715 2 29.356800602486636 3 23.13722289587414 4 29.475466978964786 5 32.618441825838524 6 25.194365851720573 7 24.126195732156 8 20.109762079960756 9 11.923552598396363 10-11 15.56464121989726 12-13 15.6428452984278 14-15 19.801803832561223 16-17 23.144261694770044 18-19 27.129063934749038 20-21 29.195923024040738 22-23 28.806133341624435 24-25 27.31753533217949 26-27 28.7588913416728 28-29 25.710897097078423 30-31 27.613639896775798 32-33 26.601024469110467 34-35 24.639660997626674 36-37 27.62793340864416 38-39 27.76551385822909 40-41 24.76726621687446 42-43 25.637335171193953 44-45 24.3343800847765 46-47 27.6588307130001 48-49 23.027214673866105 50-51 28.613214114216817 52-53 22.193743155523773 54-55 23.33132965070284 56-57 25.47345638708385 58-59 24.75089992987111 60-61 24.288152880984708 62-63 24.11481706032121 64-65 22.63563290461434 66-67 23.142016188373805 68-69 24.145843913123084 70-71 22.62483720078627 72-73 22.49405804461303 74-75 19.752014046506165 76-77 22.040336204126895 78-79 21.158132878704652 80-81 22.19147605771988 82-83 23.055132363965495 84-85 19.990167272953393 86-87 21.55081580974688 88-89 20.401764277102398 90-91 21.52060943043594 92-93 21.508172779626005 94-95 20.55799970290223 96-97 19.463228969105288 98-99 18.861865083066466 100-101 20.52349663346772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2315736.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.45983641749642 #Duplication Level Percentage of deduplicated Percentage of total 1 87.76336514289773 65.34845811989173 2 8.091162134850668 12.049332179768436 3 2.0074071708727943 4.484136286994926 4 0.7981446788118215 2.3771888888729378 5 0.4019392228730406 1.496416439245112 6 0.22385489724246152 1.0000919417957492 7 0.14346271594050714 0.7477547252657941 8 0.10377419611125993 0.6181607733441284 9 0.0750326641571102 0.5028227909196635 >10 0.36604927574509066 4.772365463911205 >50 0.018791104694643075 0.9611308935179924 >100 0.006660588366483868 0.8168294871392592 >500 1.781037181965903E-4 0.08129344691747972 >1k 1.187358121309374E-4 0.12748792871658524 >5k 0.0 0.0 >10k+ 5.93679060654687E-5 4.616530633698981 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 104434 4.509754134322738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.3182815312280845E-5 0.0 5 0.0 0.0 0.0 4.3182815312280845E-5 0.0 6 0.0 0.0 0.0 4.3182815312280845E-5 0.0 7 0.0 0.0 0.0 8.636563062456169E-5 0.0 8 0.0 0.0 0.0 8.636563062456169E-5 0.0 9 0.0 0.0 0.0 8.636563062456169E-5 0.0 10-11 0.0 0.0 0.0 8.636563062456169E-5 0.0 12-13 0.0 0.0 0.0 8.636563062456169E-5 0.0 14-15 0.0 0.0 0.0 8.636563062456169E-5 0.0 16-17 0.0 0.0 0.0 1.0795703828070212E-4 0.0 18-19 0.0 0.0 0.0 1.5113985359298298E-4 0.0 20-21 0.0 0.0 0.0 1.7273126124912338E-4 0.0 22-23 0.0 0.0 0.0 1.7273126124912338E-4 0.0 24-25 0.0 0.0 0.0 2.1591407656140424E-4 0.0 26-27 0.0 0.0 0.0 3.4546252249824676E-4 0.0 28-29 0.0 0.0 0.0 7.772906756210552E-4 0.0 30-31 0.0 0.0 0.0 0.0017704954278035148 0.0 32-33 0.0 0.0 0.0 0.0035841736709193105 0.0 34-35 0.0 0.0 0.0 0.006822884819340374 0.0 36-37 0.0 0.0 0.0 0.013559404008056185 0.0 38-39 0.0 0.0 0.0 0.02290848352316499 0.0 40-41 0.0 0.0 0.0 0.03303485371389485 0.0 42-43 0.0 0.0 0.0 0.04367941768837208 0.0 44-45 0.0 0.0 0.0 0.05579219738346686 0.0 46-47 0.0 0.0 0.0 0.07174824764135462 0.0 48-49 0.0 0.0 0.0 0.0891941050275161 0.0 50-51 0.0 0.0 0.0 0.10843204924913721 0.0 52-53 0.0 0.0 0.0 0.1302825537971513 0.0 54-55 0.0 0.0 0.0 0.15563086638546017 0.0 56-57 0.0 0.0 0.0 0.18713273015576903 0.0 58-59 0.0 2.1591407656140422E-5 0.0 0.22081532609934812 0.0 60-61 0.0 4.3182815312280845E-5 0.0 0.2568729768851026 0.0 62-63 0.0 4.3182815312280845E-5 0.0 0.29424770353788166 0.0 64-65 0.0 4.3182815312280845E-5 0.0 0.33445090459361515 0.0 66-67 0.0 4.3182815312280845E-5 0.0 0.3772234831604293 0.0 68-69 0.0 4.3182815312280845E-5 0.0 0.4279632911523593 0.0 70-71 0.0 4.3182815312280845E-5 0.0 0.48695101686893494 0.0 72-73 0.0 4.3182815312280845E-5 0.0 0.545075086279265 0.0 74-75 0.0 4.3182815312280845E-5 0.0 0.604883285486774 0.0 76-77 0.0 4.3182815312280845E-5 0.0 0.6735655532409566 0.0 78-79 0.0 4.3182815312280845E-5 0.0 0.7524605568164937 0.0 80-81 0.0 4.3182815312280845E-5 0.0 0.8390205101099608 0.0 82-83 0.0 4.3182815312280845E-5 0.0 0.9287975831441926 0.0 84-85 0.0 4.3182815312280845E-5 0.0 1.0199780976760735 0.0 86-87 0.0 4.3182815312280845E-5 0.0 1.1181542282885442 0.0 88-89 0.0 4.3182815312280845E-5 0.0 1.2258305782697163 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1595 0.0 57.516655 1 GTATCAA 3285 0.0 45.868362 1 ATCAACG 3905 0.0 37.6831 3 TCAACGC 4080 0.0 36.680798 4 CAACGCA 4030 0.0 36.002373 5 TATCAAC 4175 0.0 34.73552 2 AACGCAG 4415 0.0 33.798687 6 ACGCAGA 5190 0.0 28.44629 7 CGCAGAG 5340 0.0 27.14769 8 GTACATG 4170 0.0 26.178507 1 TACATGG 4170 0.0 25.683495 2 ACATGGG 4125 0.0 25.65548 3 GCAGAGT 5810 0.0 23.540056 9 GTCCTAC 1195 0.0 23.159391 1 GTGTAGC 805 0.0 21.646334 1 CAACGCG 145 4.1346104E-4 20.8462 5 GAGTACA 3145 0.0 20.532942 1 TAGGACG 1525 0.0 20.48634 4 GAGTACT 3760 0.0 20.053417 12-13 CATGGGG 2535 0.0 19.877626 4 >>END_MODULE