FastQCFastQC Report
Sat 18 Jun 2016
SRR1930994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315736
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53460.2308553306594534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48660.21012757930955858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.1208687000590741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.059.6095121
GTATCAA58200.040.0919571
ATCAACG72400.032.16023
CAACGCA73850.031.3458235
TCAACGC74250.031.2942244
TATCAAC75500.030.6508982
AACGCAG78250.029.9469766
CGCAGAG90000.025.4047478
ACGCAGA90200.025.3484177
ACATGGG66100.023.4356673
GTACATG69150.023.4347841
TACATGG68750.023.2926222
GTAGGAC22300.021.7348443
GACGTGG14200.021.3797177
GCAGAGT104650.021.123079
TGGTATC14750.020.9402832
GAGTACT74250.020.44390512-13
GCGTCGG2601.5152182E-820.0691819
GTGGTAT16150.020.0094491
GGCGTCG2651.8859282E-819.6905178
Produced by FastQC (version 0.11.5)