##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930994_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315736 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34008539833556 31.0 31.0 34.0 30.0 34.0 2 31.50976968013625 31.0 31.0 34.0 30.0 34.0 3 31.573208690455218 31.0 31.0 34.0 30.0 34.0 4 35.34734227044879 37.0 35.0 37.0 33.0 37.0 5 35.22151791050448 37.0 35.0 37.0 33.0 37.0 6 35.246713787754736 37.0 35.0 37.0 32.0 37.0 7 35.12944178438302 37.0 35.0 37.0 32.0 37.0 8 35.13288475024787 37.0 35.0 37.0 32.0 37.0 9 36.63188722721416 39.0 35.0 39.0 32.0 39.0 10-11 36.57071229190201 38.0 35.0 39.0 32.0 39.0 12-13 36.45841084648682 38.0 35.0 39.0 32.0 39.0 14-15 37.519521439404144 39.0 36.0 41.0 32.0 41.0 16-17 37.48057874472738 39.0 36.0 41.0 32.0 41.0 18-19 37.509368511782 39.0 36.0 41.0 32.0 41.0 20-21 37.49413901239174 39.0 36.0 41.0 32.0 41.0 22-23 37.39171563597923 39.0 36.0 41.0 32.0 41.0 24-25 37.275552567304736 39.0 36.0 40.5 31.0 41.0 26-27 37.02722460591363 39.0 36.0 40.0 31.0 41.0 28-29 36.84840586318993 39.0 36.0 40.0 30.5 41.0 30-31 36.61782107286841 38.5 35.0 40.0 30.0 41.0 32-33 36.46254516922481 38.0 35.0 40.0 30.0 41.0 34-35 36.550670931401505 38.0 35.0 40.0 30.0 41.0 36-37 36.61345183561511 39.0 35.0 40.0 30.0 41.0 38-39 36.59100951058325 39.0 35.0 40.0 30.0 41.0 40-41 36.47234378184733 39.0 35.0 40.0 30.0 41.0 42-43 36.34740639692953 39.0 35.0 40.0 30.0 41.0 44-45 36.18893993097659 38.5 35.0 40.0 30.0 41.0 46-47 35.95135110392549 38.0 35.0 40.0 29.0 41.0 48-49 35.74152990669057 38.0 35.0 40.0 28.0 41.0 50-51 35.489846208721545 38.0 34.5 40.0 27.5 41.0 52-53 35.28824896274878 38.0 34.0 40.0 27.0 41.0 54-55 35.14423794422162 38.0 34.0 40.0 27.0 41.0 56-57 34.97543588733777 38.0 34.0 40.0 26.0 41.0 58-59 34.7749089274425 38.0 33.5 40.0 26.0 41.0 60-61 34.47716492726286 38.0 33.0 40.0 26.0 41.0 62-63 34.23098487910539 37.0 33.0 40.0 25.5 41.0 64-65 33.9201530312609 37.0 32.5 40.0 25.0 41.0 66-67 33.608140349331705 36.0 32.0 39.0 24.0 41.0 68-69 33.2614246615331 36.0 32.0 39.0 23.0 40.0 70-71 32.89534644709069 35.5 31.5 39.0 22.5 40.0 72-73 32.479040141017805 35.0 31.0 38.0 22.0 40.0 74-75 31.957774547703192 35.0 31.0 37.5 21.0 39.5 76-77 30.57180481712941 33.5 29.5 36.0 20.0 39.0 78-79 31.325418786942897 34.0 30.5 36.0 20.5 39.0 80-81 31.29672812444942 34.0 31.0 36.0 21.0 38.5 82-83 31.02346899646592 34.0 31.0 36.0 20.0 37.5 84-85 30.670650929121454 34.0 30.0 35.0 19.5 37.0 86-87 30.32414640528972 34.0 30.0 35.0 18.0 36.5 88-89 29.96690339486021 34.0 30.0 35.0 17.5 36.0 90-91 29.62284517751592 34.0 29.0 35.0 11.5 36.0 92-93 29.305101704166624 34.0 29.0 35.0 7.0 35.5 94-95 28.925710011849365 34.0 29.0 35.0 2.0 35.0 96-97 28.492044861763173 34.0 29.0 35.0 2.0 35.0 98-99 27.992094306086706 34.0 29.0 35.0 2.0 35.0 100-101 26.856082688182074 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42488399637385754 1101 2 0.4675875007261716 1101 3 0.2882825771706514 1101 4 0.15279730957101378 1101 5 0.3097886475785643 1101 6 0.3524989643174692 1101 7 0.40404213280923074 1101 8 0.45238819227576954 1101 9 0.5275415049196184 1101 10-11 0.49593146129547705 1101 12-13 0.48269733440772455 1101 14-15 0.5930070886591778 1101 16-17 0.7098097760285356 1101 18-19 0.6076651607010817 1101 20-21 0.6276979843218911 1101 22-23 0.6297267412776009 1101 24-25 0.6163583793065825 1101 26-27 0.6394052649393629 1101 28-29 0.6919379434897834 1101 30-31 0.6877037188375255 1101 32-33 0.6115372373799559 1101 34-35 0.6428053114437589 1101 36-37 0.6608864077317591 1101 38-39 0.6306969069824575 1101 40-41 0.6315416400850538 1101 42-43 0.6606964950592484 1101 44-45 0.7094621344703071 1101 46-47 0.6482340355200975 1101 48-49 0.5850156556256678 1101 50-51 0.6282875912706771 1101 52-53 0.6475855494352558 1101 54-55 0.6122700514828239 1101 56-57 0.6731772977939414 1101 58-59 0.5956735406292069 1101 60-61 0.6534381522060713 1101 62-63 0.4498534261584908 1101 64-65 0.5387052398863759 1101 66-67 0.5357979691502948 1101 68-69 0.5901835412275673 1101 70-71 0.7113872069778324 1101 72-73 0.5223415826986724 1101 74-75 0.5395935309504694 1101 76-77 0.5752139564280689 1101 78-79 0.49292448688837354 1101 80-81 0.45306805115011173 1101 82-83 0.4141579168317371 1101 84-85 0.5101054138531538 1101 86-87 0.5685475100191333 1101 88-89 0.6008179715148216 1101 90-91 0.4922387527543748 1101 92-93 0.32172628328982356 1101 94-95 0.5791036934674523 1101 96-97 0.7852919930252433 1101 98-99 0.9762685656518002 1101 100-101 1.2390063959448234 1102 1 0.2852023851424619 1102 2 0.4540364005331696 1102 3 0.3852800195503683 1102 4 0.22909754291806905 1102 5 0.34305176510012103 1102 6 0.3130484230579853 1102 7 0.3603784649465638 1102 8 0.29818629984667666 1102 9 0.401906436079841 1102 10-11 0.4635122855298448 1102 12-13 0.4721438818871846 1102 14-15 0.6034253397179725 1102 16-17 0.6248683903096222 1102 18-19 0.5739631721188445 1102 20-21 0.528197891726677 1102 22-23 0.4987025599624815 1102 24-25 0.5143178334555856 1102 26-27 0.4866196967703402 1102 28-29 0.5833331887745885 1102 30-31 0.6015617085590179 1102 32-33 0.6302024218116529 1102 34-35 0.6182178582461475 1102 36-37 0.6598710625437434 1102 38-39 0.6090604035840173 1102 40-41 0.7266967846518853 1102 42-43 0.7202183159525362 1102 44-45 0.5795457156581563 1102 46-47 0.7752995714044388 1102 48-49 0.7377028469416018 1102 50-51 0.619804233236394 1102 52-53 0.3716022745676639 1102 54-55 0.5335207148149976 1102 56-57 0.576394131941413 1102 58-59 0.6009573382817237 1102 60-61 0.6089608739680443 1102 62-63 0.2876354212730874 1102 64-65 0.4127813558187796 1102 66-67 0.4124932576106204 1102 68-69 0.6091505273432674 1102 70-71 0.548390904204112 1102 72-73 0.49872989268217793 1102 74-75 0.5589220920279985 1102 76-77 0.6488829202743069 1102 78-79 0.741546810677729 1102 80-81 0.6098739948910037 1102 82-83 0.4710470450509341 1102 84-85 0.6164306897130558 1102 86-87 0.6344390097787063 1102 88-89 0.6991150379455249 1102 90-91 0.7670179340775043 1102 92-93 0.5734973779644683 1102 94-95 0.7713945950672958 1102 96-97 0.7242037920390203 1102 98-99 0.9522968127950904 1102 100-101 1.1297849624570944 1103 1 0.3394324214186639 1103 2 0.35359559129210183 1103 3 0.28400344018808 1103 4 0.15862283738879057 1103 5 0.28804965642550684 1103 6 0.26222988982054574 1103 7 0.4455108214648149 1103 8 0.3485683986866377 1103 9 0.4002366457748394 1103 10-11 0.4204183261388863 1103 12-13 0.42649860120597083 1103 14-15 0.4580697611799067 1103 16-17 0.5106811225044439 1103 18-19 0.49574385595939674 1103 20-21 0.5327996147150813 1103 22-23 0.43588391822892447 1103 24-25 0.3847194790967521 1103 26-27 0.46297637951892767 1103 28-29 0.484555257585626 1103 30-31 0.40331400841429854 1103 32-33 0.6749996828095703 1103 34-35 0.606981509945598 1103 36-37 0.5434472871937146 1103 38-39 0.5082954184322332 1103 40-41 0.6365952182828849 1103 42-43 0.5673058075747051 1103 44-45 0.5413546434889867 1103 46-47 0.6843714220857748 1103 48-49 0.48642180523884804 1103 50-51 0.5210902622608558 1103 52-53 0.5103610264422827 1103 54-55 0.5854677827073189 1103 56-57 0.5902125129342934 1103 58-59 0.7469204950988626 1103 60-61 0.6177690965740865 1103 62-63 0.5014416402007669 1103 64-65 0.44680381451588147 1103 66-67 0.48780067787034653 1103 68-69 0.3450648434064689 1103 70-71 0.38559492416040086 1103 72-73 0.46691515505905556 1103 74-75 0.48423651203748364 1103 76-77 0.5801361029730252 1103 78-79 0.5181314227778451 1103 80-81 0.43806802445444504 1103 82-83 0.5053436314479676 1103 84-85 0.3941506612240744 1103 86-87 0.4793876403651609 1103 88-89 0.5267866236863377 1103 90-91 0.5684195464698583 1103 92-93 0.4349417938266029 1103 94-95 0.47982999350179867 1103 96-97 0.4721862016678777 1103 98-99 0.5438800511943178 1103 100-101 0.8613412343203777 1104 1 0.33656587119820713 1104 2 0.3586255135824352 1104 3 0.35769484850053956 1104 4 0.18329621962029563 1104 5 0.32519120781446276 1104 6 0.20790305912514384 1104 7 0.2498593776299174 1104 8 0.25567756094906713 1104 9 0.42828656415285593 1104 10-11 0.38215466722357405 1104 12-13 0.35191125529667744 1104 14-15 0.5082228781356477 1104 16-17 0.536575805044258 1104 18-19 0.4920634229041454 1104 20-21 0.41257891510301903 1104 22-23 0.42528526468871064 1104 24-25 0.43187751068263935 1104 26-27 0.45877349652312915 1104 28-29 0.4737182297683731 1104 30-31 0.3525389890691102 1104 32-33 0.5413725507273668 1104 34-35 0.45364470284526703 1104 36-37 0.46192873745104635 1104 38-39 0.35235216085818166 1104 40-41 0.25955211529723243 1104 42-43 0.49658706671714725 1104 44-45 0.6272053038220946 1104 46-47 0.4276682924063593 1104 48-49 0.2667073696596276 1104 50-51 0.16183408694757162 1104 52-53 0.248321827116726 1104 54-55 0.39306113731026926 1104 56-57 0.4193092354178205 1104 58-59 0.45153633513016445 1104 60-61 0.44394517155205904 1104 62-63 0.305666681444535 1104 64-65 0.34176125509974753 1104 66-67 0.1937911082769972 1104 68-69 0.09602864063771932 1104 70-71 0.255938359692891 1104 72-73 0.23847660178491026 1104 74-75 0.2709460641465853 1104 76-77 0.316415906249329 1104 78-79 0.46373638622539204 1104 80-81 0.29296170814199485 1104 82-83 0.23803509688716318 1104 84-85 0.34439137250383567 1104 86-87 0.23927645096926753 1104 88-89 0.4080466316840763 1104 90-91 0.3838585654134299 1104 92-93 0.2680956395809595 1104 94-95 0.4852234495479699 1104 96-97 0.4388802776256604 1104 98-99 0.7055105347115251 1104 100-101 0.9208868517478379 1105 1 0.2948156028139479 1105 2 0.3756346050619719 1105 3 0.3306737870745948 1105 4 0.23536591161057174 1105 5 0.21239482665286857 1105 6 0.2342010602852369 1105 7 0.303828548258366 1105 8 0.3305105573247502 1105 9 0.33927328803855517 1105 10-11 0.38450350775085695 1105 12-13 0.4250305546288331 1105 14-15 0.3883792951849685 1105 16-17 0.4200257854117879 1105 18-19 0.44805070030845684 1105 20-21 0.4102690164385976 1105 22-23 0.3985115436100557 1105 24-25 0.4559877103727743 1105 26-27 0.40187501353826605 1105 28-29 0.46262983190973017 1105 30-31 0.34656957560668644 1105 32-33 0.48809430057402636 1105 34-35 0.416986720724168 1105 36-37 0.4085839970427756 1105 38-39 0.387025553413757 1105 40-41 0.21596732806349195 1105 42-43 0.2840726484499143 1105 44-45 0.29352838082103716 1105 46-47 0.1998238741612326 1105 48-49 0.27432058887459476 1105 50-51 0.2525244989677802 1105 52-53 0.2205116402995131 1105 54-55 0.29453728899034815 1105 56-57 0.18856466870045807 1105 58-59 0.3345037084811082 1105 60-61 0.32672931408540506 1105 62-63 0.2527532774342731 1105 64-65 0.1661005485787257 1105 66-67 0.16594214964873544 1105 68-69 0.09678758183398628 1105 70-71 0.03070100563772371 1105 72-73 0.023622355735938072 1105 74-75 0.1359621740041277 1105 76-77 0.1675041782696347 1105 78-79 0.29680231309869143 1105 80-81 0.24895468228170614 1105 82-83 0.38239462312963823 1105 84-85 0.31165027858439487 1105 86-87 0.34490200268108495 1105 88-89 0.46353189916110793 1105 90-91 0.4077109665703951 1105 92-93 0.4691856376270671 1105 94-95 0.5144092704245864 1105 96-97 0.5141855928838446 1105 98-99 0.6339139642482614 1105 100-101 0.5522598619079204 1106 1 0.21278942239072762 1106 2 0.22797452709363242 1106 3 0.31549661461012235 1106 4 -0.04585119160406492 1106 5 0.17102079035898043 1106 6 0.1382317494207328 1106 7 0.20232921460119968 1106 8 0.2542630016197407 1106 9 0.441594153910593 1106 10-11 0.28595401778336793 1106 12-13 0.35628390191177317 1106 14-15 0.3501267325674249 1106 16-17 0.374256626498358 1106 18-19 0.3419671770362811 1106 20-21 0.2830092423724082 1106 22-23 0.28273105064090487 1106 24-25 0.30347566015418437 1106 26-27 0.3127337862219477 1106 28-29 0.3902654980657445 1106 30-31 0.35942364029799023 1106 32-33 0.35000116203698894 1106 34-35 0.5328882763544556 1106 36-37 0.34885576528001394 1106 38-39 0.4250516840169496 1106 40-41 0.4447939419209419 1106 42-43 0.3358608137029364 1106 44-45 0.4329758767420202 1106 46-47 0.3497380591678976 1106 48-49 0.4969682741116799 1106 50-51 0.3223564809329318 1106 52-53 0.1626297386691533 1106 54-55 0.33659410872904516 1106 56-57 0.44702921552505614 1106 58-59 0.5271943634898903 1106 60-61 0.3918543000735113 1106 62-63 0.17137824473987706 1106 64-65 0.2102161808571239 1106 66-67 0.14331439242171484 1106 68-69 0.23312705548541857 1106 70-71 0.30613674403277 1106 72-73 0.3943366503466521 1106 74-75 0.5752360471374374 1106 76-77 0.5523621289229013 1106 78-79 0.5905865176597196 1106 80-81 0.46917364129227934 1106 82-83 0.4571550510836566 1106 84-85 0.503476556156663 1106 86-87 0.3680199889556697 1106 88-89 0.35008572347079436 1106 90-91 0.5345380537180731 1106 92-93 0.49023235635565854 1106 94-95 0.5901862759919752 1106 96-97 0.5022043750333296 1106 98-99 0.6218923921773687 1106 100-101 0.7795881385087 1107 1 0.04683272175497777 1107 2 0.12283088232947748 1107 3 0.12100392836127938 1107 4 0.10651820278389579 1107 5 0.13559654385682052 1107 6 0.20010588031383492 1107 7 0.21701772261227603 1107 8 0.18331484422829902 1107 9 0.1254464842384948 1107 10-11 0.12077204056263469 1107 12-13 0.20687581213226736 1107 14-15 0.12538158223487272 1107 16-17 0.2694186626858297 1107 18-19 0.24864958447766838 1107 20-21 0.3336730055593833 1107 22-23 0.2273031340099223 1107 24-25 0.2075539073006638 1107 26-27 0.3938249562997882 1107 28-29 0.29641997786576724 1107 30-31 0.4204281953356457 1107 32-33 0.3752048337969782 1107 34-35 0.3856861786719463 1107 36-37 0.3757543025297849 1107 38-39 0.2480329704378832 1107 40-41 0.11324339405226169 1107 42-43 0.0963333631783101 1107 44-45 0.19976895895698732 1107 46-47 0.14020542968371075 1107 48-49 0.12135456740389827 1107 50-51 0.04720415019723845 1107 52-53 0.1622870301956425 1107 54-55 0.09480439079882785 1107 56-57 0.09345911810353158 1107 58-59 0.05967059940577002 1107 60-61 0.06154784431691951 1107 62-63 -0.24697509079553015 1107 64-65 -0.15240885839286733 1107 66-67 -0.2052649822698953 1107 68-69 -0.124732606944292 1107 70-71 -0.10518772390049946 1107 72-73 -0.028439598210781014 1107 74-75 -0.03741456067668025 1107 76-77 -0.06237229647765474 1107 78-79 -0.1037100205932191 1107 80-81 -0.10389299601335722 1107 82-83 -0.02746743538784102 1107 84-85 0.09864185468086362 1107 86-87 -0.06973601272443375 1107 88-89 0.08522011135447372 1107 90-91 0.06633670229508581 1107 92-93 -0.009782895068553898 1107 94-95 0.20788559457139044 1107 96-97 0.2900881379756335 1107 98-99 0.40848622717653527 1107 100-101 0.6160279504349901 1108 1 0.055034036620725146 1108 2 0.10927572420481724 1108 3 0.1577825679313989 1108 4 -0.009925060623125148 1108 5 -0.03016672425118827 1108 6 0.11810453328864412 1108 7 0.13805775456847158 1108 8 0.10543292250951453 1108 9 0.2335497636006707 1108 10-11 0.1809741336804649 1108 12-13 0.14069438924553168 1108 14-15 0.17621785081444585 1108 16-17 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-1.1195643447193433 2316 90-91 -1.1196084608236099 2316 92-93 -1.0121911099207175 2316 94-95 -1.471809200174743 2316 96-97 -1.135698140794542 2316 98-99 -1.3097988340253828 2316 100-101 -1.7474435394121102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 21.0 5 50.0 6 82.0 7 282.0 8 704.0 9 1397.0 10 2435.0 11 3917.0 12 5753.0 13 7700.0 14 9462.0 15 11931.0 16 14124.0 17 16570.0 18 14339.0 19 11273.0 20 10682.0 21 11296.0 22 12759.0 23 14584.0 24 18284.0 25 23320.0 26 30532.0 27 39260.0 28 50426.0 29 63533.0 30 78238.0 31 94402.0 32 114781.0 33 139611.0 34 172188.0 35 215673.0 36 282914.0 37 389505.0 38 380286.0 39 73386.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.47833905240966 22.445913487490728 15.259828591811688 22.81591886828793 2 14.618385685857218 22.01017108427075 41.44056099886681 21.930882231005224 3 18.202522790183487 26.76601790395087 31.45615345750079 23.57530584836485 4 12.09850931429842 18.4267214344357 40.151015086801664 29.323754164464223 5 11.657510980561675 39.318939595790965 36.59835996550478 12.425189458142587 6 28.709922866388972 40.5765027062834 17.971803178447153 12.741771248880479 7 25.142460107715216 33.95758411148766 23.456300718216585 17.443655062580536 8 23.76380940207443 38.61441977300394 20.627294369836115 16.99447645508552 9 24.628411874237823 16.775141898731118 21.96601857897446 36.6304276480566 10-11 23.38330016893117 27.8770118873654 29.70608480414002 19.033603139563404 12-13 24.092750641696636 25.242536282201428 30.00965999578536 20.65505308031658 14-15 20.886793658689935 27.68642453198465 27.29061084683228 24.136170962493132 16-17 20.060987090065534 30.512977299657646 28.65007064708585 20.77596496319097 18-19 20.15510403603865 28.972646277468588 30.95780348018945 19.91444620630331 20-21 20.93723118697468 28.42072671496233 30.653019169715375 19.98902292834762 22-23 20.50736506732869 28.468961609929316 30.569315321438733 20.45435800130326 24-25 20.42680866416795 28.531474963634444 30.936081907949635 20.105634464247967 26-27 20.147554738709918 28.88309842063875 30.734452859048496 20.234893981602838 28-29 20.15000878772189 28.80887287858564 30.645506753590983 20.395611580101487 30-31 20.671338606990204 28.408304983918836 30.661848615746045 20.258507793344915 32-33 20.427785359229432 28.809384308619478 30.538567176904724 20.224263155246366 34-35 20.536960780989887 28.750263720995818 30.714636930959195 19.998138567055097 36-37 20.43831396314183 28.63597822615871 30.650000248303495 20.27570756239597 38-39 20.531543083939642 28.879322908828513 30.565141567700373 20.023992439531472 40-41 20.86617531003078 28.437781705080216 30.650203664338715 20.04583932055029 42-43 20.46362419452255 28.793644671966806 30.459350772379484 20.283380361131165 44-45 20.322258004195085 29.125825337811428 30.1654627138839 20.386453944109586 46-47 20.72812171236178 28.63363380223296 30.02724690908023 20.610997576325033 48-49 20.657270507042803 28.891376968540065 29.854544512272806 20.596808012144326 50-51 20.55837997208057 29.29660382811616 29.560914699928848 20.58410149987442 52-53 20.628216592988597 29.292528414746684 29.438609210526028 20.64064578173869 54-55 20.66301850284194 29.065485548433912 29.666417601022754 20.605078347701397 56-57 20.758899074010895 29.187744917251763 29.292103310706874 20.761252698030468 58-59 20.659836497480256 29.195215685461513 29.455828059755124 20.689119757303107 60-61 20.547926845086256 29.15822128064335 29.781897185351724 20.511954688918674 62-63 20.737341251634014 29.04848372044705 29.66293837057301 20.551236657345925 64-65 20.507598216452042 29.097302319863367 29.543146021550015 20.851953442134576 66-67 20.573563628673334 29.375383814142282 29.125608339327524 20.92544421785686 68-69 20.686534547940187 29.003356287226843 29.606518288150657 20.70359087668231 70-71 20.89148727946227 28.64232168804395 29.616840791250038 20.84935024124374 72-73 20.82413058790378 29.15328110088227 29.29534200692766 20.727246304286286 74-75 20.508249291620324 29.60343945478311 29.26372899029346 20.624582263303108 76-77 20.551147367168312 29.180863149011678 29.525235586233755 20.742753897586255 78-79 20.434162056650298 29.226380953614818 29.432698079555713 20.906758910179175 80-81 20.57543729646037 29.242536223388306 29.31145604126898 20.87057043888235 82-83 20.582700273260855 29.25581758887887 29.548791399365037 20.612690738495235 84-85 20.69907796052745 29.06736778285608 29.363796218567227 20.86975803804924 86-87 20.227910262655154 29.49984367820857 29.224315725108564 21.04793033402771 88-89 20.57090753946697 29.157882554732105 29.289633438037356 20.981576467763567 90-91 20.564509250680118 29.069071701704946 29.651688675849506 20.714730371765423 92-93 20.56066168531076 29.350386558977238 29.386213449660954 20.702738306051053 94-95 20.18334163184244 29.233416404792205 29.448385936380596 21.134856026984767 96-97 20.455967543782 29.052287934500278 29.466648817761964 21.025095703955763 98-99 20.390320831044644 29.127413487547805 29.454264216646457 21.028001464761097 100-101 20.281456953642383 29.218011034072966 29.33875018568611 21.161781826598542 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 75.0 1 60.5 2 72.5 3 103.5 4 108.0 5 120.5 6 230.5 7 485.5 8 836.5 9 1112.5 10 1119.5 11 1057.0 12 1143.0 13 1306.0 14 1486.0 15 1710.5 16 2009.5 17 2371.0 18 2722.5 19 3067.5 20 3581.0 21 4256.5 22 5251.5 23 6780.0 24 8679.5 25 11144.5 26 15509.0 27 20913.5 28 25815.0 29 32005.5 30 38524.5 31 45354.5 32 55099.5 33 65995.0 34 76996.0 35 86466.0 36 96949.5 37 105510.0 38 104568.0 39 100068.5 40 98104.5 41 95827.0 42 92325.5 43 91813.0 44 92340.5 45 92118.0 46 89982.5 47 86415.0 48 84063.0 49 80694.0 50 75503.5 51 69256.0 52 61820.0 53 54899.0 54 48759.5 55 41744.5 56 33746.0 57 26271.0 58 20699.0 59 16100.5 60 11812.0 61 8077.5 62 5282.5 63 3248.0 64 1838.5 65 1039.5 66 569.0 67 301.5 68 172.0 69 108.0 70 73.0 71 45.0 72 25.0 73 13.5 74 6.5 75 4.5 76 3.5 77 2.5 78 2.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0040591846393544 2 0.006563787927466689 3 0.001986409504364919 4 0.16901753913226722 5 0.002288689211550885 6 6.045594143719319E-4 7 0.0 8 5.61376599059651E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.0227970718596595E-4 24-25 6.261508220280723E-4 26-27 0.002288689211550885 28-29 2.1591407656140424E-4 30-31 0.0059808199207508975 32-33 0.022001644401607092 34-35 0.013624178231024606 36-37 9.284305292140382E-4 38-39 2.806882995298255E-4 40-41 0.0016625383895228127 42-43 0.011292306204161441 44-45 0.007297895787775463 46-47 0.010536606936196527 48-49 0.010838886643382492 50-51 0.19183965702480765 52-53 0.11367876130957934 54-55 0.017964051169908832 56-57 0.006822884819340374 58-59 0.018136782431157957 60-61 0.12246646422562849 62-63 0.2364690966500499 64-65 0.24275219627798678 66-67 0.2531160719529342 68-69 0.24799890833842891 70-71 0.23392131054662535 72-73 0.159754825247783 74-75 0.2770825344512501 76-77 0.21638908752983932 78-79 0.005441034729347387 80-81 6.477422296842127E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 8.63656306245617E-5 90-91 0.006455830889185987 92-93 0.01882770747615445 94-95 0.00254778610342457 96-97 4.318281531228085E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2315736.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.69512155806885 #Duplication Level Percentage of deduplicated Percentage of total 1 72.86060497578734 39.12259040969338 2 15.480126027516977 16.62414497563499 3 5.132993047632009 8.268500569479691 4 2.3065135459461468 4.9539410089964315 5 1.1993337976230183 3.2199187026034157 6 0.7283733326112861 2.3466056780531233 7 0.46426944905967127 1.7450303152069695 8 0.32343530175878044 1.3893518275286716 9 0.22667222689895 1.0954073499459556 >10 1.1265726835785361 11.035296983047694 >50 0.09502681202473645 3.526339499391023 >100 0.05349189238254544 5.14739966283779 >500 0.0017784986862031575 0.5999797413142778 >1k 7.275676443554509E-4 0.6934362876205613 >5k 8.084084937282787E-5 0.23205698864597937 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5346 0.2308553306594534 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4866 0.21012757930955858 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2799 0.1208687000590741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.3182815312280845E-5 0.0 10-11 0.0 0.0 0.0 4.3182815312280845E-5 0.0 12-13 0.0 0.0 0.0 4.3182815312280845E-5 0.0 14-15 0.0 0.0 0.0 4.3182815312280845E-5 0.0 16-17 0.0 0.0 0.0 6.477422296842126E-5 0.0 18-19 0.0 0.0 0.0 1.2954844593684252E-4 0.0 20-21 0.0 0.0 0.0 1.7273126124912338E-4 0.0 22-23 0.0 0.0 0.0 1.7273126124912338E-4 0.0 24-25 0.0 0.0 0.0 2.1591407656140424E-4 0.0 26-27 0.0 0.0 0.0 3.886453378105276E-4 0.0 28-29 0.0 0.0 0.0 0.001122753198119302 0.0 30-31 0.0 0.0 0.0 0.0029796142565473786 0.0 32-33 0.0 0.0 0.0 0.007103573118870199 0.0 34-35 0.0 0.0 0.0 0.013861683715242153 0.0 36-37 0.0 0.0 0.0 0.026276743117522892 0.0 38-39 0.0 0.0 0.0 0.045233999039614184 0.0 40-41 0.0 0.0 0.0 0.06768906300200023 0.0 42-43 0.0 0.0 0.0 0.08956115895767047 0.0 44-45 0.0 0.0 0.0 0.11564357940628811 0.0 46-47 0.0 0.0 0.0 0.14857047608190227 0.0 48-49 0.0 0.0 0.0 0.18434743856812694 0.0 50-51 0.0 0.0 0.0 0.22288810123433758 0.0 52-53 0.0 0.0 0.0 0.26123444123164297 0.0 54-55 0.0 0.0 0.0 0.3058422894492291 0.0 56-57 0.0 0.0 0.0 0.35995035703551703 0.0 58-59 0.0 0.0 0.0 0.4198233304659944 0.0 60-61 0.0 0.0 0.0 0.4828702408219244 0.0 62-63 0.0 0.0 0.0 0.5497820131483035 0.0 64-65 0.0 0.0 0.0 0.6202995505532582 4.3182815312280845E-5 66-67 0.0 0.0 0.0 0.6955887890502199 4.3182815312280845E-5 68-69 0.0 0.0 0.0 0.7880000138185008 4.3182815312280845E-5 70-71 0.0 0.0 0.0 0.8941649652637433 4.3182815312280845E-5 72-73 0.0 0.0 0.0 1.0003299167089859 4.3182815312280845E-5 74-75 0.0 0.0 0.0 1.107833535428909 4.3182815312280845E-5 76-77 0.0 0.0 0.0 1.2358921742374778 4.3182815312280845E-5 78-79 0.0 0.0 0.0 1.3804898313106504 4.3182815312280845E-5 80-81 0.0 0.0 0.0 1.5432458622226368 4.3182815312280845E-5 82-83 0.0 0.0 0.0 1.7112270137874093 4.3182815312280845E-5 84-85 0.0 0.0 0.0 1.8818854999015433 4.3182815312280845E-5 86-87 0.0 0.0 0.0 2.0686727675348138 4.3182815312280845E-5 88-89 0.0 0.0 0.0 2.2746116137590815 4.3182815312280845E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3165 0.0 59.609512 1 GTATCAA 5820 0.0 40.091957 1 ATCAACG 7240 0.0 32.1602 3 CAACGCA 7385 0.0 31.345823 5 TCAACGC 7425 0.0 31.294224 4 TATCAAC 7550 0.0 30.650898 2 AACGCAG 7825 0.0 29.946976 6 CGCAGAG 9000 0.0 25.404747 8 ACGCAGA 9020 0.0 25.348417 7 ACATGGG 6610 0.0 23.435667 3 GTACATG 6915 0.0 23.434784 1 TACATGG 6875 0.0 23.292622 2 GTAGGAC 2230 0.0 21.734844 3 GACGTGG 1420 0.0 21.379717 7 GCAGAGT 10465 0.0 21.12307 9 TGGTATC 1475 0.0 20.940283 2 GAGTACT 7425 0.0 20.443905 12-13 GCGTCGG 260 1.5152182E-8 20.069181 9 GTGGTAT 1615 0.0 20.009449 1 GGCGTCG 265 1.8859282E-8 19.690517 8 >>END_MODULE