FastQCFastQC Report
Sat 18 Jun 2016
SRR1930993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291791
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1002944.376228024283192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.057.6091961
GTATCAA36550.047.201211
TATCAAC46250.037.079112
ATCAACG45350.036.4089933
CAACGCA45950.035.703995
TCAACGC46750.035.6382184
AACGCAG50800.032.0562446
ACGCAGA57750.027.8684397
GTACATG34500.027.7480931
CGCAGAG58300.027.197048
TACATGG37400.025.1850282
GCAGAGT62350.024.3772039
GTGGTAT9800.024.0293671
ACATGGG39000.023.491943
GAGTACT41800.021.08012212-13
CAGAGTA60500.020.52903710-11
GTATAGG5450.019.7257461
AGAGTAC56350.019.64164510-11
TGGTATC11000.019.5457732
GTACTTT45100.019.2272214-15
Produced by FastQC (version 0.11.5)