FastQCFastQC Report
Sat 18 Jun 2016
SRR1930993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291791
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73990.3228479385772961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65480.28571540773133325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40100.17497232513785071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.059.1396061
GTATCAA65150.040.1252751
ATCAACG84500.030.8772013
TCAACGC85100.030.7144
CAACGCA85100.030.5462285
TATCAAC85800.030.2451762
AACGCAG89150.029.1572746
GTGGTAT15350.027.5582351
ACGCAGA102450.025.1868957
TGGTATC16500.025.060292
CGCAGAG103350.024.8757678
GTACATG59350.021.4626731
GCAGAGT117350.021.3421659
ACGGGCG2951.364242E-1020.9039335
GAGTACT79900.020.89698212-13
TACATGG60800.020.4808732
AGAGTAC106100.020.36388610-11
ACATGGG61250.019.7859253
GGCGTCG2902.4847395E-919.6277688
CAGAGTA113100.019.5438910-11
Produced by FastQC (version 0.11.5)