##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930993_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2291791 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.325872647200377 31.0 31.0 34.0 30.0 34.0 2 31.47635800995815 31.0 31.0 34.0 30.0 34.0 3 31.538348392152688 31.0 31.0 34.0 30.0 34.0 4 35.322662494093045 37.0 35.0 37.0 33.0 37.0 5 35.19110774062731 37.0 35.0 37.0 32.0 37.0 6 35.22463828507922 37.0 35.0 37.0 32.0 37.0 7 35.10166328430472 37.0 35.0 37.0 32.0 37.0 8 35.10339380859773 37.0 35.0 37.0 32.0 37.0 9 36.59558615947091 39.0 35.0 39.0 32.0 39.0 10-11 36.533774022151235 38.0 35.0 39.0 32.0 39.0 12-13 36.41469750077559 38.0 35.0 39.0 32.0 39.0 14-15 37.464737404065204 39.0 36.0 41.0 32.0 41.0 16-17 37.4186531407096 39.0 36.0 41.0 32.0 41.0 18-19 37.4368954237101 39.0 36.0 41.0 32.0 41.0 20-21 37.422680776737494 39.0 36.0 41.0 32.0 41.0 22-23 37.32713301518332 39.0 36.0 40.5 31.5 41.0 24-25 37.20276718077696 39.0 36.0 40.0 31.0 41.0 26-27 36.9426642743601 39.0 36.0 40.0 31.0 41.0 28-29 36.75109815860172 39.0 35.5 40.0 30.0 41.0 30-31 36.51170023793618 38.0 35.0 40.0 30.0 41.0 32-33 36.34143165759879 38.0 35.0 40.0 30.0 41.0 34-35 36.4161537854019 38.0 35.0 40.0 30.0 41.0 36-37 36.48518211302863 38.0 35.0 40.0 30.0 41.0 38-39 36.472915942160526 38.5 35.0 40.0 30.0 41.0 40-41 36.35059501499046 38.0 35.0 40.0 30.0 41.0 42-43 36.2023816744197 38.0 35.0 40.0 30.0 41.0 44-45 36.0214951974242 38.0 35.0 40.0 29.5 41.0 46-47 35.75907183508444 38.0 35.0 40.0 28.5 41.0 48-49 35.517499196043616 38.0 34.0 40.0 27.5 41.0 50-51 35.23596392515722 38.0 34.0 40.0 26.5 41.0 52-53 35.01829682549587 38.0 34.0 40.0 26.0 41.0 54-55 34.86029943393616 38.0 34.0 40.0 26.0 41.0 56-57 34.6744631164011 38.0 33.0 40.0 26.0 41.0 58-59 34.45205758291223 37.5 33.0 40.0 26.0 41.0 60-61 34.13136952715148 37.0 33.0 40.0 25.0 41.0 62-63 33.85595588777511 37.0 32.5 40.0 24.5 41.0 64-65 33.53005967821673 36.0 32.0 39.0 23.5 41.0 66-67 33.19604492730794 36.0 32.0 39.0 23.0 40.5 68-69 32.83383606969396 35.5 31.0 39.0 22.0 40.0 70-71 32.439012981550235 35.0 31.0 38.0 22.0 40.0 72-73 31.99979688374725 35.0 31.0 37.5 21.5 39.5 74-75 31.478928706849793 35.0 30.0 37.0 20.0 39.0 76-77 30.110404700952223 33.5 28.5 35.5 18.5 38.0 78-79 30.839121673835006 34.0 30.0 36.0 20.0 38.5 80-81 30.82269412874036 34.0 30.0 36.0 20.0 37.5 82-83 30.562722560652347 34.0 30.0 35.0 18.5 37.0 84-85 30.237085318861972 34.0 30.0 35.0 18.0 36.5 86-87 29.902577503795065 34.0 30.0 35.0 16.0 36.0 88-89 29.561338926629873 34.0 29.0 35.0 9.5 36.0 90-91 29.22806355378828 34.0 29.0 35.0 7.0 35.5 92-93 28.91145505851101 34.0 29.0 35.0 2.0 35.0 94-95 28.521052748701777 34.0 29.0 35.0 2.0 35.0 96-97 28.08457664769606 33.0 29.0 35.0 2.0 35.0 98-99 27.59082612681523 33.0 27.5 35.0 2.0 35.0 100-101 26.450711255956588 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24093154135140438 1101 2 0.355886477225539 1101 3 0.22810722303709952 1101 4 0.09783286881895492 1101 5 0.22008523669247637 1101 6 0.2851471687424265 1101 7 0.35343096109023975 1101 8 0.2657650247605119 1101 9 0.42797416649469966 1101 10-11 0.36782197339400824 1101 12-13 0.3837879035348095 1101 14-15 0.48006476890889616 1101 16-17 0.5692242772311786 1101 18-19 0.4778429205745738 1101 20-21 0.5077732255592977 1101 22-23 0.49945108865583165 1101 24-25 0.3988285174655175 1101 26-27 0.4520246552586116 1101 28-29 0.48513358239765125 1101 30-31 0.43702548120576523 1101 32-33 0.47078150363822857 1101 34-35 0.5181600583299826 1101 36-37 0.6653225628868569 1101 38-39 0.5735224127130536 1101 40-41 0.5006253552468038 1101 42-43 0.5486491097040158 1101 44-45 0.5429592686080511 1101 46-47 0.5060107914310166 1101 48-49 0.4856833136037082 1101 50-51 0.4094663790334465 1101 52-53 0.20656542740304218 1101 54-55 0.47405767702861823 1101 56-57 0.4454736015016749 1101 58-59 0.4468008809460855 1101 60-61 0.49333773111222 1101 62-63 0.23205609098787505 1101 64-65 0.4230008553263005 1101 66-67 0.4365074998206211 1101 68-69 0.3694767615491372 1101 70-71 0.3258102868917021 1101 72-73 0.41903214988140647 1101 74-75 0.34033519596503936 1101 76-77 0.21284540865955037 1101 78-79 0.18361627336100028 1101 80-81 0.10624175781618206 1101 82-83 0.18751793407763273 1101 84-85 0.3154533730613345 1101 86-87 0.40803587596797186 1101 88-89 0.26194929603247274 1101 90-91 0.26995660969534185 1101 92-93 0.11808149941686708 1101 94-95 0.15728927179920404 1101 96-97 0.3678729218357688 1101 98-99 0.20572859802385324 1101 100-101 0.4610301860536943 1102 1 0.3502506437586774 1102 2 0.41324678373187496 1102 3 0.38427499948135235 1102 4 0.1825494572049493 1102 5 0.37671894661931304 1102 6 0.3287328658827775 1102 7 0.4994138355103459 1102 8 0.36998662734088583 1102 9 0.4849374197778715 1102 10-11 0.4939282047855187 1102 12-13 0.5145924177522829 1102 14-15 0.5502122808529535 1102 16-17 0.538590953899309 1102 18-19 0.6282665882644665 1102 20-21 0.5803324481572147 1102 22-23 0.5209301894474905 1102 24-25 0.554258085056162 1102 26-27 0.6203112101204766 1102 28-29 0.5985012933515605 1102 30-31 0.6264110332287558 1102 32-33 0.656093022725635 1102 34-35 0.6627703763764714 1102 36-37 0.7992577029265533 1102 38-39 0.6479924314080918 1102 40-41 0.7253210559646064 1102 42-43 0.7646563410493599 1102 44-45 0.8458993377335347 1102 46-47 0.9367257252482659 1102 48-49 0.7879188927460419 1102 50-51 0.6972865099564274 1102 52-53 0.6842089147204149 1102 54-55 0.6856035592152452 1102 56-57 0.6554408274808523 1102 58-59 0.6410823408325328 1102 60-61 0.6090316834975624 1102 62-63 0.5015973484601162 1102 64-65 0.5173366981610243 1102 66-67 0.3967163476522302 1102 68-69 0.5214843145761208 1102 70-71 0.5523397892805022 1102 72-73 0.5501121851365589 1102 74-75 0.5823095638534248 1102 76-77 0.6241961541531218 1102 78-79 0.6088391785356038 1102 80-81 0.4499721035013202 1102 82-83 0.42592333082792777 1102 84-85 0.6713775081272324 1102 86-87 0.48239806640349414 1102 88-89 0.5830973748863038 1102 90-91 0.484628790148637 1102 92-93 0.6299245764819794 1102 94-95 0.7760406421917985 1102 96-97 0.9686979342414261 1102 98-99 1.0158639495367652 1102 100-101 1.2037187809252536 1103 1 0.331515059314917 1103 2 0.36798764626741587 1103 3 0.3107196237634291 1103 4 0.20725768491935526 1103 5 0.27048521764318423 1103 6 0.31163758619243964 1103 7 0.32517686089314424 1103 8 0.3342697674201318 1103 9 0.49383527913913383 1103 10-11 0.36676560373388867 1103 12-13 0.4353401319588812 1103 14-15 0.4137479886139417 1103 16-17 0.466319062235776 1103 18-19 0.4785185750421235 1103 20-21 0.35535708662647636 1103 22-23 0.31849438862765567 1103 24-25 0.4102911023890883 1103 26-27 0.546392144905063 1103 28-29 0.5601940043610512 1103 30-31 0.5599191908635817 1103 32-33 0.5692661471333125 1103 34-35 0.5856981758489468 1103 36-37 0.5877082554277209 1103 38-39 0.6237421830608838 1103 40-41 0.6043022829139844 1103 42-43 0.635778681307869 1103 44-45 0.5999537129262151 1103 46-47 0.6425407072930511 1103 48-49 0.5001181107377946 1103 50-51 0.5324791467472139 1103 52-53 0.6872370735827005 1103 54-55 0.6281640795739989 1103 56-57 0.43250781842617414 1103 58-59 0.486911089019884 1103 60-61 0.26860636036074226 1103 62-63 0.27740467313270045 1103 64-65 0.4567206222467135 1103 66-67 0.3637907176495929 1103 68-69 0.2884158135655994 1103 70-71 0.35608305250570993 1103 72-73 0.2871293532076784 1103 74-75 0.42896381672796835 1103 76-77 0.3325598172458726 1103 78-79 0.44171961719669994 1103 80-81 0.36368117259893395 1103 82-83 0.4121791327324935 1103 84-85 0.3405290741419158 1103 86-87 0.3830568644601051 1103 88-89 0.40676042125015854 1103 90-91 0.3245874810163123 1103 92-93 0.3781333365728692 1103 94-95 0.35895774417971893 1103 96-97 0.48243437148665436 1103 98-99 0.5999717621973382 1103 100-101 0.8520666297246251 1104 1 0.31374415371600506 1104 2 0.38578634632078135 1104 3 0.26945458696356894 1104 4 0.11305932562600418 1104 5 0.2838468804742149 1104 6 0.3384901560676994 1104 7 0.2407011754818953 1104 8 0.3488828755627935 1104 9 0.4948391800908212 1104 10-11 0.3862423939747046 1104 12-13 0.4524091407252513 1104 14-15 0.5099995763721523 1104 16-17 0.5319397714668739 1104 18-19 0.5193138855617647 1104 20-21 0.45433227034782675 1104 22-23 0.483551322093291 1104 24-25 0.4199119790817676 1104 26-27 0.42329285130112737 1104 28-29 0.5072572944184159 1104 30-31 0.5012943458176551 1104 32-33 0.4643700497526595 1104 34-35 0.4806213276566922 1104 36-37 0.5024660510773344 1104 38-39 0.7469322429301428 1104 40-41 0.6644213404596258 1104 42-43 0.628315435999582 1104 44-45 0.7308955592840292 1104 46-47 0.571594146300896 1104 48-49 0.5880278009964002 1104 50-51 0.36909483143580246 1104 52-53 0.2762197166407603 1104 54-55 0.43987654107868224 1104 56-57 0.4342959572817122 1104 58-59 0.4996738682151829 1104 60-61 0.4021449036255902 1104 62-63 0.2957996510916914 1104 64-65 0.08604891366600498 1104 66-67 0.1600131559737008 1104 68-69 0.11095021962589868 1104 70-71 0.19997159048961066 1104 72-73 0.046511545022763556 1104 74-75 0.14030320716778633 1104 76-77 0.3457855478231089 1104 78-79 0.41542145983005696 1104 80-81 0.3404081810262092 1104 82-83 0.38895009114087387 1104 84-85 0.6198016724231046 1104 86-87 0.5738865532456003 1104 88-89 0.5785465658535891 1104 90-91 0.5731723089964333 1104 92-93 0.5961463492415788 1104 94-95 0.76619444731271 1104 96-97 0.7682845274135452 1104 98-99 0.7231730267060428 1104 100-101 0.9593031070276723 1105 1 0.3459946573162078 1105 2 0.34577825657279604 1105 3 0.35019134334964974 1105 4 0.20713211192807535 1105 5 0.2896101562328326 1105 6 0.24154979518993258 1105 7 0.26157379677454173 1105 8 0.3132598578189203 1105 9 0.5670267872397972 1105 10-11 0.38927069202031106 1105 12-13 0.36717145750049696 1105 14-15 0.4563467337097151 1105 16-17 0.46792562244407776 1105 18-19 0.5423187715552515 1105 20-21 0.4396434845600652 1105 22-23 0.4619186127241903 1105 24-25 0.5046842339249054 1105 26-27 0.4220398779169372 1105 28-29 0.4068299039246739 1105 30-31 0.31940158587423184 1105 32-33 0.4648026637591727 1105 34-35 0.601626679748243 1105 36-37 0.5828097046432532 1105 38-39 0.5422227773892487 1105 40-41 0.5130760850164577 1105 42-43 0.4903082195157822 1105 44-45 0.5016362533951266 1105 46-47 0.4937544304599939 1105 48-49 0.6035751179859474 1105 50-51 0.47017516102607004 1105 52-53 0.33109271831228426 1105 54-55 0.5591434009706759 1105 56-57 0.548785312030553 1105 58-59 0.6244923846304005 1105 60-61 0.5380761086230166 1105 62-63 0.40242694943731294 1105 64-65 0.3913538596629209 1105 66-67 0.5926760492104606 1105 68-69 0.39759851917158784 1105 70-71 0.446473845975639 1105 72-73 0.3859313868708689 1105 74-75 0.4845105993759127 1105 76-77 0.4892948644269133 1105 78-79 0.6743262097443363 1105 80-81 0.4949866569391226 1105 82-83 0.6127561836318698 1105 84-85 0.7482933898069568 1105 86-87 0.7482266494653835 1105 88-89 0.7104516479297054 1105 90-91 0.7403146724886334 1105 92-93 0.7952780406953757 1105 94-95 0.8331250576332998 1105 96-97 0.8965298021435295 1105 98-99 0.8477772451613816 1105 100-101 0.8589453633708928 1106 1 0.20270252746056272 1106 2 0.24064236112745263 1106 3 0.2646240985804482 1106 4 0.19612695515744605 1106 5 0.20623880738916966 1106 6 0.16795276760267797 1106 7 0.20088803698454427 1106 8 0.3045049524842156 1106 9 0.457283345248797 1106 10-11 0.34509400126380996 1106 12-13 0.33038761635997105 1106 14-15 0.3396522890429594 1106 16-17 0.2953477106560669 1106 18-19 0.3993814531293296 1106 20-21 0.2649083592956245 1106 22-23 0.3713601648774656 1106 24-25 0.4373329212560009 1106 26-27 0.29374103198779977 1106 28-29 0.340036152073921 1106 30-31 0.45752410652932696 1106 32-33 0.44127806572318207 1106 34-35 0.4448393771954642 1106 36-37 0.4817221275784789 1106 38-39 0.49427621519187426 1106 40-41 0.34792803794658056 1106 42-43 0.3780687591492864 1106 44-45 0.4193406767998695 1106 46-47 0.3201652727503159 1106 48-49 0.29881544270509863 1106 50-51 0.28730621716363913 1106 52-53 0.24239636353286897 1106 54-55 0.3131565383307233 1106 56-57 0.46870864074866603 1106 58-59 0.3899882069258922 1106 60-61 0.4676388702297558 1106 62-63 0.28932897212924047 1106 64-65 0.3895277812896083 1106 66-67 0.47009370227958414 1106 68-69 0.3428653547071505 1106 70-71 0.4929366361273537 1106 72-73 0.5138905942274654 1106 74-75 0.647106605212965 1106 76-77 0.5518937381007056 1106 78-79 0.44333063021455743 1106 80-81 0.4575670564087737 1106 82-83 0.5950086092882039 1106 84-85 0.5983840601337853 1106 86-87 0.6887478821283075 1106 88-89 0.8258607348371676 1106 90-91 0.8034312573886062 1106 92-93 0.8317059032633054 1106 94-95 1.0163789919051496 1106 96-97 0.9955010157320068 1106 98-99 1.0446697439675638 1106 100-101 1.1845490430055143 1107 1 0.1067888446711045 1107 2 0.1375805892666726 1107 3 0.11758353759023876 1107 4 -0.04223000007940669 1107 5 -0.0017218264897422841 1107 6 0.12301639311615986 1107 7 0.13294274204791634 1107 8 0.16313537219593854 1107 9 0.21239601616832005 1107 10-11 0.16960431617858518 1107 12-13 0.1520083591528163 1107 14-15 0.20006943526282583 1107 16-17 0.1342229044631651 1107 18-19 0.115862585520496 1107 20-21 0.15272924587837622 1107 22-23 0.20112198875814613 1107 24-25 0.1699583073068922 1107 26-27 0.1389465815887263 1107 28-29 0.1579459558483549 1107 30-31 0.22102122650602496 1107 32-33 0.32594725260037194 1107 34-35 0.20472020125293255 1107 36-37 0.24233026695260662 1107 38-39 0.19009667452863965 1107 40-41 0.1637564540638934 1107 42-43 0.12595973908730684 1107 44-45 0.2788053078286836 1107 46-47 0.06197399701879647 1107 48-49 0.252155296271944 1107 50-51 0.30209318737776414 1107 52-53 0.3135028224849705 1107 54-55 0.2001552129893227 1107 56-57 0.1756776346553437 1107 58-59 0.1490412418765814 1107 60-61 0.07612648026503166 1107 62-63 0.025337933478688512 1107 64-65 9.536370406877381E-4 1107 66-67 0.037212898164767694 1107 68-69 -0.01461853146017944 1107 70-71 -0.11782806997371154 1107 72-73 -0.05289236514731854 1107 74-75 0.13650522606020488 1107 76-77 0.14263466850321294 1107 78-79 0.12017227507105943 1107 80-81 0.020663329059313895 1107 82-83 0.1150642425299715 1107 84-85 0.1633310278871889 1107 86-87 0.18121538996932074 1107 88-89 0.18954653259229914 1107 90-91 0.1437199289814437 1107 92-93 -0.13175822683309946 1107 94-95 0.0725478967121056 1107 96-97 0.12561989760491343 1107 98-99 0.28719057305463025 1107 100-101 0.4983268304729407 1108 1 0.07493839969643545 1108 2 0.18945480993990316 1108 3 0.04144637540272811 1108 4 0.05280181183230326 1108 5 0.08052218167254921 1108 6 0.10529920494911238 1108 7 0.0804949165914266 1108 8 0.09279324077250095 1108 9 0.0809524689179213 1108 10-11 0.14464355081336322 1108 12-13 0.16597320194556175 1108 14-15 0.2624614618521335 1108 16-17 0.2693444346527869 1108 18-19 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-0.9458230021625944 2316 86-87 -0.7868129991702766 2316 88-89 -0.8413525882551873 2316 90-91 -1.0161340425328298 2316 92-93 -0.9633083540177694 2316 94-95 -1.5944492035753903 2316 96-97 -1.23595213431922 2316 98-99 -1.2648978661936034 2316 100-101 -1.8519762406933111 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 31.0 5 59.0 6 115.0 7 313.0 8 817.0 9 1466.0 10 2515.0 11 4099.0 12 5944.0 13 7645.0 14 9668.0 15 12640.0 16 15284.0 17 17952.0 18 15802.0 19 12330.0 20 12014.0 21 12668.0 22 13985.0 23 16112.0 24 19657.0 25 24978.0 26 32644.0 27 41411.0 28 52771.0 29 65440.0 30 79991.0 31 96843.0 32 116598.0 33 141760.0 34 175397.0 35 219433.0 36 286333.0 37 385943.0 38 337408.0 39 53700.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.65697138104584 21.288101660864562 15.14178569776646 22.91314126032313 2 15.458257768685776 21.58104515725883 40.2167272638412 22.74396981021419 3 18.623696245702515 26.050344998815316 30.950056878006166 24.375901877476004 4 13.069043558379278 17.90767850945707 38.29608403797688 30.727193894186765 5 12.58544780189811 38.410777789898546 35.71759572379186 13.286178684411476 6 30.125867339681246 39.038641613629245 17.23936611661339 13.596124930076126 7 26.19558240694723 33.56265907318774 22.497644855050044 17.744113664814986 8 24.66062390832624 37.62317396223632 19.922819783233898 17.79338234620354 9 25.06485102699155 16.90882807376414 21.026655571995875 36.99966532724843 10-11 24.165663448368548 27.395539122022903 28.524285155147215 19.914512274461327 12-13 24.690471338791365 24.648386349366064 29.273066348545747 21.388075963296828 14-15 21.736711593683715 26.554559294455732 26.63622031851944 25.072508793341104 16-17 21.062631801940054 29.793074499376253 27.60487321924207 21.539420479441624 18-19 21.023208486288674 28.437671672504166 30.039410225452496 20.499709615754664 20-21 21.482630833265336 27.475323884245988 30.36162547108353 20.68041981140514 22-23 21.30713738104765 27.21730585715289 30.271745224997698 21.20381153680176 24-25 21.300031067608703 27.480652559412437 30.235110237649753 20.984206135329107 26-27 21.12019038921093 27.699334977363137 30.116573683639825 21.06390094978611 28-29 21.121378208358806 27.85723542371084 30.087416500748105 20.93396986718225 30-31 21.40321541107263 27.551904718475896 30.072161947870775 20.972717922580706 32-33 21.343246387893103 27.72281678437164 29.855166580690103 21.078770247045156 34-35 21.454474678964903 27.686675156033573 30.00664631895174 20.852203846049782 36-37 21.399777421091066 27.594679487025648 30.101932064344112 20.903611027539178 38-39 21.291116158609153 27.697477664239372 30.103899384955263 20.907506792196212 40-41 21.297573823368477 27.64175387061613 30.048294886798494 21.012377419216897 42-43 21.290727716612093 27.757654280770023 29.708631012838723 21.242986989779165 44-45 21.30668355132993 27.904684272215448 29.6791230331651 21.10950914328952 46-47 21.514194578674847 27.954431945204178 29.322538365215163 21.208835110905817 48-49 21.4880395083623 28.19157907552719 29.086185841076766 21.23419557503375 50-51 21.478843761632668 28.301120517672473 28.95166471072073 21.26837100997412 52-53 21.575461247197744 28.304956954921046 28.83858663269888 21.280995165182322 54-55 21.43841878672692 28.228444993355605 29.001813297954747 21.331322921962727 56-57 21.440808395344664 28.400354155148406 28.86772462299015 21.291112826516777 58-59 21.463216650155584 28.273100607055106 28.980400542901908 21.283282199887406 60-61 21.434788905200815 28.377043884276237 29.087337151649294 21.100830058873655 62-63 21.427977882343267 28.33080180660477 29.004823531521335 21.23639677953063 64-65 21.1479003497704 28.189692160935497 29.06508038770848 21.597327101585623 66-67 21.194219966961366 28.295732732196132 28.963023019655864 21.547024281186637 68-69 21.264626738237375 28.360191302339576 28.934981874042087 21.44020008538096 70-71 21.267551612872186 28.23064260354719 28.908565860587736 21.593239922992886 72-73 21.32779352183814 28.383077353048698 28.780273446249165 21.508855678864 74-75 21.301024705559595 28.571691103276965 28.67206577997993 21.45521841118351 76-77 21.238651889389494 28.417070435628833 28.698588191515157 21.645689483466516 78-79 21.089720121256498 28.41823717278789 28.845632728258114 21.6464099776975 80-81 21.27727207177096 28.598774626214745 28.60813412046171 21.51581918155259 82-83 21.36191738251874 28.49845382934133 28.705911664719864 21.433717123420067 84-85 21.259530210215505 28.591568777432148 28.4851236434736 21.66377736887875 86-87 21.185156063532844 28.66341215232977 28.54195692364618 21.609474860491204 88-89 21.200939004009967 28.492313868199094 28.478612787384534 21.828134340406407 90-91 21.209967813668367 28.46753613980458 28.628576889376067 21.693919157150987 92-93 21.25549966275936 28.41573708191598 28.593118457984357 21.735644797340306 94-95 21.060945224786515 28.43495961566153 28.598962355949435 21.905132803602516 96-97 21.060498330890194 28.296827305986298 28.607152355330907 22.035522007792597 98-99 20.867566021508942 28.412625758631567 28.489639762089997 22.23016845776949 100-101 20.90256048653651 28.357865093282943 28.549374703016113 22.190199717164436 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 341.0 1 257.5 2 258.5 3 382.0 4 353.5 5 329.0 6 575.0 7 1276.5 8 2122.0 9 2613.5 10 2367.0 11 1844.0 12 1798.0 13 1975.0 14 2259.5 15 2564.0 16 2790.5 17 3082.0 18 3424.0 19 3803.5 20 4164.0 21 4584.0 22 5291.5 23 6396.5 24 7950.0 25 10136.0 26 12941.0 27 15842.0 28 19371.5 29 24463.5 30 30619.0 31 36892.0 32 44126.5 33 50295.5 34 54988.0 35 59469.0 36 66043.0 37 74079.0 38 78876.5 39 81475.0 40 83510.5 41 85273.5 42 87448.0 43 91016.0 44 93821.0 45 96609.5 46 99047.0 47 99828.0 48 99399.0 49 97161.0 50 92097.5 51 86254.5 52 81127.5 53 74771.0 54 67452.0 55 57042.0 56 46070.0 57 36632.0 58 28061.0 59 21063.0 60 15279.0 61 11116.0 62 7993.5 63 5110.5 64 2864.5 65 1576.5 66 857.0 67 468.0 68 244.5 69 129.0 70 78.5 71 48.0 72 27.0 73 17.0 74 14.0 75 10.0 76 10.0 77 7.0 78 1.5 79 1.5 80 2.0 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004057961655316737 2 0.006763269425527895 3 0.0019198958369240474 4 0.1689944676456099 5 0.0017889938480428625 6 5.672419518184687E-4 7 0.0 8 5.236079555247402E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.236079555247402E-4 24-25 6.545099444059253E-4 26-27 0.0028143927609454787 28-29 1.745359851749134E-4 30-31 0.00610875948112197 32-33 0.0204643442617586 34-35 0.012959296899237322 36-37 8.508629277277029E-4 38-39 2.8362097590923434E-4 40-41 0.0016144578628679493 42-43 0.01075578008640404 44-45 0.0071996093884651785 46-47 0.010014002149410657 48-49 0.009839466164235745 50-51 0.18856431498334708 52-53 0.11183393250082578 54-55 0.016842722569379145 56-57 0.00610875948112197 58-59 0.01749723251378507 60-61 0.11940443085778764 62-63 0.23091110838641044 64-65 0.2371071358601199 66-67 0.2478192819502302 68-69 0.24291045736718575 70-71 0.22903484654578013 72-73 0.1582386875592059 74-75 0.27725041245035 76-77 0.2138720328337095 78-79 0.005236079555247402 80-81 2.1816998146864176E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 4.363399629372835E-5 90-91 0.005890589499653328 92-93 0.017475415515638207 94-95 0.0026616737739174295 96-97 2.1816998146864176E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2291791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.98725382792194 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5344303920223 37.18878590225877 2 15.352692616266845 15.96288655967855 3 5.376972378409125 8.386020835862409 4 2.549705524959662 5.302087532501316 5 1.3203628646266614 3.4321019694154193 6 0.8759013845275596 2.7321424563397496 7 0.585604855789709 2.131079179656226 8 0.4187512056464021 1.7415780174950275 9 0.31096190545862123 1.4549449958902447 >10 1.554843040284359 14.375639626280368 >50 0.08569189007000022 3.0163893469095258 >100 0.03222815385900397 2.896058154607358 >500 0.0013482095126110447 0.42947469891545537 >1k 3.370523781525184E-4 0.3398474820669207 >5k 1.685261890762592E-4 0.6109632421226274 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7399 0.3228479385772961 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6548 0.28571540773133325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4010 0.17497232513785071 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.363399629372836E-5 0.0 0.0 0.0 6 0.0 4.363399629372836E-5 0.0 0.0 0.0 7 0.0 4.363399629372836E-5 0.0 0.0 0.0 8 0.0 4.363399629372836E-5 0.0 0.0 0.0 9 0.0 4.363399629372836E-5 0.0 0.0 0.0 10-11 0.0 4.363399629372836E-5 0.0 0.0 0.0 12-13 0.0 6.545099444059254E-5 0.0 0.0 4.363399629372836E-5 14-15 0.0 8.726799258745672E-5 0.0 0.0 4.363399629372836E-5 16-17 0.0 8.726799258745672E-5 0.0 0.0 4.363399629372836E-5 18-19 0.0 8.726799258745672E-5 0.0 0.0 4.363399629372836E-5 20-21 0.0 8.726799258745672E-5 0.0 1.0908499073432088E-4 4.363399629372836E-5 22-23 0.0 8.726799258745672E-5 0.0 1.7453598517491343E-4 4.363399629372836E-5 24-25 0.0 8.726799258745672E-5 0.0 1.7453598517491343E-4 4.363399629372836E-5 26-27 0.0 8.726799258745672E-5 0.0 3.490719703498268E-4 4.363399629372836E-5 28-29 0.0 8.726799258745672E-5 0.0 9.599479184620237E-4 4.363399629372836E-5 30-31 0.0 8.726799258745672E-5 0.0 0.0029889287461203925 4.363399629372836E-5 32-33 0.0 8.726799258745672E-5 0.0 0.00861771426801135 8.726799258745672E-5 34-35 0.0 1.0908499073432088E-4 0.0 0.016210029623120084 8.726799258745672E-5 36-37 0.0 2.1816998146864178E-4 0.0 0.03034744442228807 8.726799258745672E-5 38-39 0.0 2.1816998146864178E-4 0.0 0.05465158035789476 8.726799258745672E-5 40-41 0.0 2.1816998146864178E-4 0.0 0.08163920706556575 8.726799258745672E-5 42-43 0.0 2.618039777623701E-4 0.0 0.10441615313089195 8.726799258745672E-5 44-45 0.0 2.618039777623701E-4 0.0 0.1328218847181091 8.726799258745672E-5 46-47 0.0 2.618039777623701E-4 0.0 0.16932172261781286 8.726799258745672E-5 48-49 0.0 2.618039777623701E-4 0.0 0.21055584911538616 8.726799258745672E-5 50-51 0.0 2.618039777623701E-4 0.0 0.2546261853720518 8.726799258745672E-5 52-53 0.0 2.618039777623701E-4 0.0 0.3030162872617966 8.726799258745672E-5 54-55 0.0 3.0543797405609846E-4 0.0 0.35448258589024917 8.726799258745672E-5 56-57 0.0 3.0543797405609846E-4 0.0 0.4179264165013302 1.0908499073432088E-4 58-59 0.0 3.0543797405609846E-4 0.0 0.4910133602933252 1.3090198888118505E-4 60-61 0.0 3.0543797405609846E-4 0.0 0.5689000436776303 1.3090198888118505E-4 62-63 0.0 3.0543797405609846E-4 0.0 0.6509537737079865 1.3090198888118505E-4 64-65 0.0 3.4907197034982686E-4 0.0 0.7383744852824712 1.3090198888118505E-4 66-67 0.0 3.4907197034982686E-4 0.0 0.8431178933855661 1.3090198888118505E-4 68-69 0.0 3.4907197034982686E-4 0.0 0.964420403082131 1.3090198888118505E-4 70-71 0.0 3.4907197034982686E-4 0.0 1.0988131116668143 1.3090198888118505E-4 72-73 0.0 3.4907197034982686E-4 0.0 1.2441797703193704 1.3090198888118505E-4 74-75 0.0 3.4907197034982686E-4 0.0 1.393604390627243 1.3090198888118505E-4 76-77 0.0 3.4907197034982686E-4 0.0 1.5486577964570067 1.3090198888118505E-4 78-79 0.0 3.4907197034982686E-4 0.0 1.7351713136145488 1.3090198888118505E-4 80-81 0.0 3.4907197034982686E-4 0.0 1.9548466679553242 1.3090198888118505E-4 82-83 0.0 3.4907197034982686E-4 0.0 2.1886376200971203 1.3090198888118505E-4 84-85 0.0 3.7088896849669104E-4 0.0 2.4202468724242308 1.3090198888118505E-4 86-87 0.0 4.799739592310119E-4 0.0 2.668633396326279 1.3090198888118505E-4 88-89 0.0 4.799739592310119E-4 0.0 2.9519925682577512 1.3090198888118505E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3480 0.0 59.139606 1 GTATCAA 6515 0.0 40.125275 1 ATCAACG 8450 0.0 30.877201 3 TCAACGC 8510 0.0 30.714 4 CAACGCA 8510 0.0 30.546228 5 TATCAAC 8580 0.0 30.245176 2 AACGCAG 8915 0.0 29.157274 6 GTGGTAT 1535 0.0 27.558235 1 ACGCAGA 10245 0.0 25.186895 7 TGGTATC 1650 0.0 25.06029 2 CGCAGAG 10335 0.0 24.875767 8 GTACATG 5935 0.0 21.462673 1 GCAGAGT 11735 0.0 21.342165 9 ACGGGCG 295 1.364242E-10 20.903933 5 GAGTACT 7990 0.0 20.896982 12-13 TACATGG 6080 0.0 20.480873 2 AGAGTAC 10610 0.0 20.363886 10-11 ACATGGG 6125 0.0 19.785925 3 GGCGTCG 290 2.4847395E-9 19.627768 8 CAGAGTA 11310 0.0 19.54389 10-11 >>END_MODULE