FastQCFastQC Report
Sat 18 Jun 2016
SRR1930992_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930992_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3200677
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1471934.598808314615939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.059.580931
GTATCAA45500.047.7467161
ATCAACG51400.039.8713533
CAACGCA51900.039.4759645
TCAACGC54750.037.42554
TATCAAC57200.036.4504172
AACGCAG57550.034.45256
GTACATG54400.029.8333991
ACGCAGA66200.029.3400237
CGCAGAG68000.028.4877538
TACATGG58050.027.158682
ACATGGG58000.026.7182753
GCAGAGT73650.024.4292139
GAGTACA38200.022.990441
GAGTACT45650.021.94262912-13
TATCGGC1653.468249E-521.7864552
AGAGTAC64800.021.30984910-11
CAGAGTA69150.020.6305510-11
CATGGGG38450.020.2138984
AGTACAT42400.020.1054922
Produced by FastQC (version 0.11.5)