##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930992_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3200677 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.78185458888854 28.0 2.0 31.0 2.0 31.0 2 20.073726589718362 28.0 2.0 31.0 2.0 33.0 3 20.853897472316014 30.0 2.0 31.0 2.0 33.0 4 22.411884735635617 32.0 2.0 35.0 2.0 37.0 5 21.547852220014704 30.0 2.0 35.0 2.0 37.0 6 22.233376251336825 30.0 2.0 35.0 2.0 37.0 7 21.997015943814386 30.0 2.0 35.0 2.0 37.0 8 21.77036108298338 29.0 2.0 35.0 2.0 37.0 9 20.692010471534616 26.0 2.0 33.0 2.0 37.0 10-11 20.705056930143215 26.0 2.0 33.5 2.0 37.0 12-13 20.371377680409488 25.5 2.0 33.0 2.0 37.0 14-15 21.91873531755938 28.5 2.0 35.0 2.0 37.5 16-17 23.112705686953106 31.5 2.0 36.5 2.0 38.5 18-19 23.1743117159276 32.0 2.0 37.5 2.0 39.5 20-21 23.423106736481063 32.0 2.0 38.0 2.0 40.0 22-23 23.363909260447087 32.0 2.0 38.0 2.0 40.0 24-25 23.414457785024858 32.0 2.0 38.0 2.0 40.0 26-27 23.268962003976036 32.0 2.0 38.0 2.0 40.0 28-29 23.21224103525598 31.5 2.0 38.0 2.0 40.0 30-31 23.386986721871654 32.0 2.0 38.0 2.0 40.0 32-33 23.343554504250193 31.5 2.0 38.0 2.0 40.0 34-35 23.105874475931188 31.0 2.0 38.0 2.0 40.0 36-37 22.60393691709598 30.0 2.0 38.0 2.0 40.0 38-39 22.632465100352206 30.0 2.0 38.0 2.0 40.0 40-41 22.644097326909275 30.0 2.0 38.0 2.0 40.0 42-43 22.58831819018289 30.0 2.0 38.0 2.0 40.0 44-45 22.25621641921381 30.0 2.0 38.0 2.0 40.0 46-47 21.96091451902207 30.0 2.0 38.0 2.0 40.0 48-49 21.865747309084924 29.5 2.0 38.0 2.0 40.0 50-51 19.072013670857757 17.5 2.0 35.5 2.0 38.5 52-53 21.31301502775819 28.0 2.0 36.5 2.0 39.0 54-55 22.28496221268188 30.5 2.0 38.0 2.0 40.0 56-57 22.339673606552616 31.0 2.0 38.0 2.0 40.0 58-59 22.089066000724223 30.0 2.0 38.0 2.0 40.0 60-61 21.845461288346186 30.0 2.0 38.0 2.0 40.0 62-63 21.43060936795559 28.5 2.0 37.0 2.0 40.0 64-65 21.150128550928443 28.0 2.0 36.5 2.0 39.5 66-67 20.8738941792627 28.0 2.0 36.0 2.0 39.0 68-69 20.4838652884999 27.5 2.0 35.5 2.0 39.0 70-71 20.20078580250366 26.0 2.0 35.0 2.0 39.0 72-73 19.84420999057387 26.0 2.0 35.0 2.0 38.0 74-75 19.44014766251015 26.0 2.0 34.5 2.0 37.5 76-77 19.104735341929224 25.0 2.0 34.0 2.0 37.0 78-79 18.810568045447887 25.0 2.0 34.0 2.0 36.0 80-81 18.449670960237476 24.0 2.0 34.0 2.0 36.0 82-83 18.052473586056948 22.0 2.0 34.0 2.0 35.5 84-85 17.739584781594644 20.5 2.0 33.5 2.0 35.0 86-87 17.311599858404954 19.0 2.0 33.0 2.0 35.0 88-89 16.96110260423029 18.0 2.0 33.0 2.0 35.0 90-91 16.54642470952239 12.0 2.0 32.0 2.0 35.0 92-93 16.046359723271046 4.5 2.0 32.0 2.0 34.5 94-95 15.526103383752876 2.0 2.0 32.0 2.0 34.0 96-97 14.91255334418312 2.0 2.0 31.5 2.0 34.0 98-99 14.222368580147263 2.0 2.0 31.0 2.0 34.0 100-101 13.026495019647406 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 9.261955164376655 1101 2 10.322524420671076 1101 3 9.832978821485963 1101 4 11.920215823674067 1101 5 12.644750923468298 1101 6 11.916798922224828 1101 7 12.096906830803466 1101 8 11.956152607827772 1101 9 13.027165301631243 1101 10-11 12.580843753706866 1101 12-13 12.083990074022537 1101 14-15 11.411183424690979 1101 16-17 11.027831359365774 1101 18-19 11.743542892006094 1101 20-21 11.842571921222074 1101 22-23 12.218358465096077 1101 24-25 12.213871462546006 1101 26-27 12.379356767806254 1101 28-29 12.512622454872883 1101 30-31 12.179954179162618 1101 32-33 12.060972802632673 1101 34-35 12.055742291324577 1101 36-37 12.436058291487008 1101 38-39 12.1725062904605 1101 40-41 11.859571952242817 1101 42-43 11.75196530878145 1101 44-45 11.717068331448704 1101 46-47 11.53469923189687 1101 48-49 11.672286163435597 1101 50-51 13.734005493412045 1101 52-53 12.286561249466331 1101 54-55 11.873177653488991 1101 56-57 11.962023793397197 1101 58-59 11.857717048481316 1101 60-61 11.552560482521596 1101 62-63 11.601938841068417 1101 64-65 11.395759922240597 1101 66-67 11.16533786583905 1101 68-69 11.226994824840258 1101 70-71 11.12136918900488 1101 72-73 11.071841010911701 1101 74-75 10.881662743781234 1101 76-77 10.702471610365812 1101 78-79 10.455275806873537 1101 80-81 10.316875786260042 1101 82-83 10.112975678016593 1101 84-85 9.820982677613824 1101 86-87 9.815666249128178 1101 88-89 9.711714506773156 1101 90-91 9.379859786052716 1101 92-93 9.215420221431318 1101 94-95 9.115687430236912 1101 96-97 9.231841392937923 1101 98-99 9.049341454633533 1101 100-101 8.341149003858797 1102 1 9.001050445065658 1102 2 9.79795050795498 1102 3 9.255075506323365 1102 4 11.322019355845097 1102 5 12.14526353782988 1102 6 11.2929291280944 1102 7 11.337610799602505 1102 8 11.174091236199256 1102 9 11.675127667134497 1102 10-11 10.926852830776337 1102 12-13 10.492761720467001 1102 14-15 9.881655167545397 1102 16-17 10.181617438966025 1102 18-19 11.064291517460791 1102 20-21 11.439575358825117 1102 22-23 11.850654971363536 1102 24-25 11.901026915356038 1102 26-27 12.115401056877435 1102 28-29 11.839738833615243 1102 30-31 12.014744744062629 1102 32-33 11.91050773540378 1102 34-35 11.77368815600937 1102 36-37 11.947693929044668 1102 38-39 11.959564007497779 1102 40-41 11.681222991508974 1102 42-43 11.389406378371305 1102 44-45 11.348882079472379 1102 46-47 11.287065354534466 1102 48-49 11.045551345878746 1102 50-51 13.253030671936447 1102 52-53 11.978889957834 1102 54-55 11.469454170528937 1102 56-57 11.338328935875648 1102 58-59 11.41402528182702 1102 60-61 11.448373713579006 1102 62-63 11.439843500262349 1102 64-65 11.199329489322839 1102 66-67 11.302744575591639 1102 68-69 11.227945362665327 1102 70-71 10.934580814633048 1102 72-73 10.883350837825162 1102 74-75 10.432753190410537 1102 76-77 10.280720801062603 1102 78-79 9.9327984514738 1102 80-81 9.59842251938339 1102 82-83 9.749996332984924 1102 84-85 9.604230146776501 1102 86-87 9.460159131328485 1102 88-89 9.2545204879555 1102 90-91 9.120987964742493 1102 92-93 9.120038274646456 1102 94-95 8.928584807574316 1102 96-97 8.954763159181322 1102 98-99 8.830292735319295 1102 100-101 8.190743345243975 1103 1 8.941643091778143 1103 2 9.908954282489823 1103 3 9.259512907897587 1103 4 11.591132332781445 1103 5 11.955184905374775 1103 6 11.161130945987473 1103 7 11.00256052901305 1103 8 10.590364265311411 1103 9 10.723352917922021 1103 10-11 9.751359626364895 1103 12-13 9.338758584608822 1103 14-15 9.30099236964993 1103 16-17 10.069752901352697 1103 18-19 11.058154173952548 1103 20-21 11.713647642863648 1103 22-23 12.123568492379636 1103 24-25 12.331411994610452 1103 26-27 12.29927075897091 1103 28-29 12.06623870356907 1103 30-31 11.758506928208991 1103 32-33 11.840405178386273 1103 34-35 11.987775622754171 1103 36-37 12.504786755461794 1103 38-39 12.043946076761014 1103 40-41 11.758834153944761 1103 42-43 11.529435081946176 1103 44-45 11.481156736988776 1103 46-47 11.384031320787361 1103 48-49 11.415477197435333 1103 50-51 13.509860947668745 1103 52-53 12.046806460816214 1103 54-55 11.604403600022035 1103 56-57 11.47248137162325 1103 58-59 11.43356573946171 1103 60-61 11.444013454705999 1103 62-63 11.509464714357684 1103 64-65 11.155957640361269 1103 66-67 11.066224857002709 1103 68-69 11.057870634053746 1103 70-71 10.722118359626808 1103 72-73 10.623657727125632 1103 74-75 10.5491526765192 1103 76-77 10.253834185100011 1103 78-79 10.003806021685214 1103 80-81 9.871486687459097 1103 82-83 9.624640476015063 1103 84-85 9.502138837155002 1103 86-87 9.368884516587197 1103 88-89 9.132143007920906 1103 90-91 9.134927734435394 1103 92-93 8.834919047627137 1103 94-95 8.895866321400264 1103 96-97 8.668413483952584 1103 98-99 8.218162500346265 1103 100-101 7.565958855597762 1104 1 8.636814439417549 1104 2 9.906703267977946 1104 3 9.24448634913131 1104 4 11.221192991401143 1104 5 11.631303225032038 1104 6 10.863193433320802 1104 7 10.775813706874299 1104 8 10.199405086158745 1104 9 9.813590509298788 1104 10-11 8.971863842303925 1104 12-13 8.235766186967297 1104 14-15 9.009276725193338 1104 16-17 9.989533408585064 1104 18-19 11.246964143269754 1104 20-21 11.815203463404636 1104 22-23 12.18620759008856 1104 24-25 12.290808189189352 1104 26-27 12.42299101291702 1104 28-29 12.143856992590663 1104 30-31 11.895044202388785 1104 32-33 11.905168977397455 1104 34-35 11.807407900492414 1104 36-37 12.068644681380036 1104 38-39 11.65722640253598 1104 40-41 11.346762051725321 1104 42-43 11.107150933782663 1104 44-45 11.319910383811386 1104 46-47 11.282725298757956 1104 48-49 11.316254636387878 1104 50-51 13.351952703846546 1104 52-53 11.92443959635311 1104 54-55 11.385209176655973 1104 56-57 11.137911849869571 1104 58-59 11.257755661741381 1104 60-61 11.139697124953106 1104 62-63 11.12289798280625 1104 64-65 10.923522953245683 1104 66-67 10.969348988010246 1104 68-69 10.681568264738427 1104 70-71 10.418969248768118 1104 72-73 10.270819214793597 1104 74-75 10.270694734576143 1104 76-77 9.931353261693893 1104 78-79 9.636361130536095 1104 80-81 9.591434983880376 1104 82-83 9.474544327154003 1104 84-85 9.149577931093383 1104 86-87 8.988262282634398 1104 88-89 8.76880848058211 1104 90-91 8.503547856883795 1104 92-93 8.187168412526207 1104 94-95 8.13139167594919 1104 96-97 7.676507427045657 1104 98-99 7.525834613877334 1104 100-101 6.941937665142893 1105 1 8.611747003077454 1105 2 9.475196110734384 1105 3 8.838794722027298 1105 4 10.99024690450717 1105 5 10.918117172665472 1105 6 10.519303165733405 1105 7 10.031720231115418 1105 8 9.482805855832176 1105 9 8.817687941322959 1105 10-11 8.275855624061947 1105 12-13 7.786242287528367 1105 14-15 8.617355149161114 1105 16-17 9.913399129344665 1105 18-19 10.928616063856719 1105 20-21 11.636651366584033 1105 22-23 12.157268858251417 1105 24-25 12.261693223716758 1105 26-27 12.552975907176833 1105 28-29 12.088062862249707 1105 30-31 11.749735523781386 1105 32-33 11.780965175786175 1105 34-35 11.921094330502484 1105 36-37 12.171403277265519 1105 38-39 11.975985394047992 1105 40-41 11.624257645884441 1105 42-43 11.390049077934584 1105 44-45 11.437640746349157 1105 46-47 11.430955769193869 1105 48-49 11.331949320605982 1105 50-51 13.259670118607016 1105 52-53 11.841866495360449 1105 54-55 11.640542492006286 1105 56-57 11.425724304912013 1105 58-59 11.411451622754182 1105 60-61 11.308839154876853 1105 62-63 11.424599176879042 1105 64-65 11.286468932241515 1105 66-67 11.091643891621235 1105 68-69 10.874701896589958 1105 70-71 10.681477341128929 1105 72-73 10.575099624087223 1105 74-75 10.642972554314312 1105 76-77 10.28875102820231 1105 78-79 10.228430167365325 1105 80-81 9.818338936549058 1105 82-83 9.620270211918786 1105 84-85 9.197153369879171 1105 86-87 9.24462647483696 1105 88-89 9.15345457466929 1105 90-91 8.78261921352313 1105 92-93 8.608208550635831 1105 94-95 8.202516783847331 1105 96-97 8.155261832886545 1105 98-99 7.765671507849424 1105 100-101 6.9817848993538 1106 1 8.368356046754059 1106 2 9.25099533491844 1106 3 8.984066961615031 1106 4 10.753762610908002 1106 5 11.044609139943216 1106 6 10.295411449082223 1106 7 9.695334146521912 1106 8 9.09252344334027 1106 9 7.863130726657079 1106 10-11 7.594345757400912 1106 12-13 7.34275127418039 1106 14-15 8.335269228601287 1106 16-17 9.79091481110525 1106 18-19 11.305498931137283 1106 20-21 11.796496643399664 1106 22-23 11.951808666339051 1106 24-25 11.775891025102244 1106 26-27 12.069548940884705 1106 28-29 12.126064467386144 1106 30-31 11.747261378446144 1106 32-33 11.945746247144353 1106 34-35 11.713280290486722 1106 36-37 11.973472354452866 1106 38-39 11.666435008252034 1106 40-41 11.52690832102735 1106 42-43 11.337149272240566 1106 44-45 10.955281934994925 1106 46-47 10.850158269827478 1106 48-49 11.126318846949925 1106 50-51 13.315086926075121 1106 52-53 12.03371171585551 1106 54-55 11.45287467428783 1106 56-57 11.390931300631646 1106 58-59 11.209163863327529 1106 60-61 11.179365013061645 1106 62-63 11.21182496607112 1106 64-65 11.058862345550065 1106 66-67 10.801889396535206 1106 68-69 10.671260093345897 1106 70-71 10.51148230437975 1106 72-73 10.215346220212368 1106 74-75 10.033092429141828 1106 76-77 9.76485218347814 1106 78-79 9.466386804041285 1106 80-81 9.149244589022942 1106 82-83 9.05980752078083 1106 84-85 9.092815365458005 1106 86-87 8.886480055417625 1106 88-89 8.852590757823805 1106 90-91 8.678881639250367 1106 92-93 8.430693132877954 1106 94-95 8.39036025136444 1106 96-97 7.9338537656679335 1106 98-99 7.663755024224633 1106 100-101 7.06787996496727 1107 1 7.7445867694187704 1107 2 8.607174445546569 1107 3 8.516371622352644 1107 4 10.09628616803867 1107 5 10.461201689080731 1107 6 9.878204008679276 1107 7 9.649963724164813 1107 8 8.159009271981244 1107 9 4.94550700373053 1107 10-11 6.468123998803147 1107 12-13 6.735139618199767 1107 14-15 7.4376570199094125 1107 16-17 9.236285294680368 1107 18-19 11.025957826648796 1107 20-21 11.497061563271401 1107 22-23 11.229898072980792 1107 24-25 11.481919303279025 1107 26-27 11.510614179877226 1107 28-29 11.556925624746007 1107 30-31 11.23706822860245 1107 32-33 11.630956857241369 1107 34-35 11.765695594921297 1107 36-37 12.176956368067053 1107 38-39 11.919972010433671 1107 40-41 11.297116082737446 1107 42-43 11.045764842501278 1107 44-45 10.56283114859701 1107 46-47 11.073679815334064 1107 48-49 11.254655250804145 1107 50-51 13.135215445433392 1107 52-53 11.60551537882688 1107 54-55 11.248982117856624 1107 56-57 10.89614633241867 1107 58-59 10.973497799920704 1107 60-61 10.969939790783389 1107 62-63 11.22885087755931 1107 64-65 11.120271801215186 1107 66-67 11.041639270207455 1107 68-69 10.83646894036399 1107 70-71 10.651122046837298 1107 72-73 10.364404748310825 1107 74-75 10.385619884985708 1107 76-77 10.099601183481813 1107 78-79 10.005932200787392 1107 80-81 9.674689683369522 1107 82-83 9.490612751108376 1107 84-85 9.395174480497332 1107 86-87 9.103466315120897 1107 88-89 9.144293083986064 1107 90-91 8.86496911000346 1107 92-93 8.458770514551832 1107 94-95 8.23534083738179 1107 96-97 8.089817992306834 1107 98-99 7.759395627932237 1107 100-101 7.386300020619695 1108 1 6.946796505461862 1108 2 8.332208948525523 1108 3 8.128772432995575 1108 4 9.388147156770632 1108 5 10.225316616303594 1108 6 9.275509106019534 1108 7 8.519727781740894 1108 8 5.917579769025899 1108 9 5.388125169189433 1108 10-11 4.8734787752817965 1108 12-13 3.871083802145826 1108 14-15 6.724846380414135 1108 16-17 8.625675591090467 1108 18-19 8.840649549239263 1108 20-21 9.36052287636356 1108 22-23 10.221510296844269 1108 24-25 9.925342015962247 1108 26-27 8.615058635769 1108 28-29 10.433548037451864 1108 30-31 10.55630970824473 1108 32-33 11.062171332051296 1108 34-35 10.489153413714874 1108 36-37 10.452077477279346 1108 38-39 10.015160095133066 1108 40-41 10.336178606213423 1108 42-43 9.658849617018134 1108 44-45 9.208894374796177 1108 46-47 10.157484900439986 1108 48-49 10.571735674189501 1108 50-51 12.411070958333323 1108 52-53 11.190055511532961 1108 54-55 10.488644252667005 1108 56-57 10.558763690840546 1108 58-59 10.69757562061167 1108 60-61 10.574488560502676 1108 62-63 10.55766815192915 1108 64-65 10.38908055018332 1108 66-67 9.942544739986708 1108 68-69 10.222209283071404 1108 70-71 10.271515958172262 1108 72-73 10.003905389862329 1108 74-75 9.984498618658769 1108 76-77 9.603011472946243 1108 78-79 9.327261073094817 1108 80-81 9.226927773027043 1108 82-83 9.234899980734877 1108 84-85 9.00561693854321 1108 86-87 8.672481717078991 1108 88-89 8.607896830526641 1108 90-91 8.48898627413547 1108 92-93 8.249686883623598 1108 94-95 7.976456111029586 1108 96-97 7.8465248348217145 1108 98-99 7.59924544177713 1108 100-101 7.039599235175212 1109 1 6.361677530043284 1109 2 7.962736422248039 1109 3 8.069789987568672 1109 4 8.702653416103164 1109 5 9.261915203405142 1109 6 8.583565722341064 1109 7 6.596766718009889 1109 8 6.809700496973935 1109 9 4.836546010487222 1109 10-11 4.013079876154713 1109 12-13 4.755569942942117 1109 14-15 6.746584856173602 1109 16-17 6.647488517981966 1109 18-19 6.536422774437206 1109 20-21 9.243851260230251 1109 22-23 10.423311554148217 1109 24-25 9.955809886952661 1109 26-27 10.219906742911707 1109 28-29 10.610572125320928 1109 30-31 8.152087743487716 1109 32-33 7.2416129903875195 1109 34-35 9.090940031751288 1109 36-37 7.57183212315546 1109 38-39 7.918205271912679 1109 40-41 8.686886728497313 1109 42-43 7.352286624249938 1109 44-45 7.8959440604701925 1109 46-47 7.6890866872671495 1109 48-49 8.28491436108903 1109 50-51 10.628574503127393 1109 52-53 10.351550264401212 1109 54-55 10.290265028715492 1109 56-57 9.209485197597612 1109 58-59 9.171628564568376 1109 60-61 9.40569571450359 1109 62-63 10.055143296544202 1109 64-65 9.938391877212009 1109 66-67 9.800361899717846 1109 68-69 9.421906250233437 1109 70-71 8.91199144158179 1109 72-73 8.428289808662495 1109 74-75 7.5511647973498555 1109 76-77 7.504563862702103 1109 78-79 8.321181385287655 1109 80-81 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-18.667717282636833 2314 2 -17.990577698789426 2314 3 -18.737990992258418 2314 4 -20.31838404787378 2314 5 -19.454799494001197 2314 6 -20.12623254855231 2314 7 -19.907267864122677 2314 8 -19.696063191786873 2314 9 -18.65106205867704 2314 10-11 -18.6498884235571 2314 12-13 -18.322239855328775 2314 14-15 -19.83785318417222 2314 16-17 -21.00669015636962 2314 18-19 -21.066026793286138 2314 20-21 -21.313497442939777 2314 22-23 -21.26847518936763 2314 24-25 -21.305717490568956 2314 26-27 -21.17202409282316 2314 28-29 -21.115508566321722 2314 30-31 -21.289699000028133 2314 32-33 -21.25132649088049 2314 34-35 -21.002864002830847 2314 36-37 -20.50597767511543 2314 38-39 -20.542169367856776 2314 40-41 -20.545534020782227 2314 42-43 -20.492093779208275 2314 44-45 -20.168609253650846 2314 46-47 -19.87618708794899 2314 48-49 -19.779062584155927 2314 50-51 -17.03943702425013 2314 52-53 -19.234472790353834 2314 54-55 -20.18639203180324 2314 56-57 -20.241091171278466 2314 58-59 -20.003875758198195 2314 60-61 -19.75395846417076 2314 62-63 -19.35278177550191 2314 64-65 -19.066507258234676 2314 66-67 -18.792433489331145 2314 68-69 -18.39985167483861 2314 70-71 -18.110486944585357 2314 72-73 -17.751537280674683 2314 74-75 -17.362235779019024 2314 76-77 -17.02151682894055 2314 78-79 -16.736599594539435 2314 80-81 -16.380619396784276 2314 82-83 -15.992676216652644 2314 84-85 -15.683352582501783 2314 86-87 -15.244064001353518 2314 88-89 -14.892973996759281 2314 90-91 -14.482856976953062 2314 92-93 -13.998934306507026 2314 94-95 -13.489001073295524 2314 96-97 -12.88119650044679 2314 98-99 -12.197126111198196 2314 100-101 -11.010328195884297 2315 1 -18.667717282636833 2315 2 -17.990577698789426 2315 3 -18.737990992258418 2315 4 -20.31838404787378 2315 5 -19.454799494001197 2315 6 -20.12623254855231 2315 7 -19.907267864122677 2315 8 -19.696063191786873 2315 9 -18.65106205867704 2315 10-11 -18.6498884235571 2315 12-13 -18.322239855328775 2315 14-15 -19.83785318417222 2315 16-17 -21.00669015636962 2315 18-19 -21.066026793286138 2315 20-21 -21.313497442939777 2315 22-23 -21.26847518936763 2315 24-25 -21.305717490568956 2315 26-27 -21.17202409282316 2315 28-29 -21.115508566321722 2315 30-31 -21.289699000028133 2315 32-33 -21.25132649088049 2315 34-35 -21.002864002830847 2315 36-37 -20.50597767511543 2315 38-39 -20.542169367856776 2315 40-41 -20.545534020782227 2315 42-43 -20.492093779208275 2315 44-45 -20.168609253650846 2315 46-47 -19.87618708794899 2315 48-49 -19.779062584155927 2315 50-51 -17.03943702425013 2315 52-53 -19.234472790353834 2315 54-55 -20.18639203180324 2315 56-57 -20.241091171278466 2315 58-59 -20.003875758198195 2315 60-61 -19.75395846417076 2315 62-63 -19.35278177550191 2315 64-65 -19.066507258234676 2315 66-67 -18.792433489331145 2315 68-69 -18.39985167483861 2315 70-71 -18.110486944585357 2315 72-73 -17.751537280674683 2315 74-75 -17.362235779019024 2315 76-77 -17.02151682894055 2315 78-79 -16.736599594539435 2315 80-81 -16.380619396784276 2315 82-83 -15.992676216652644 2315 84-85 -15.683352582501783 2315 86-87 -15.244064001353518 2315 88-89 -14.892973996759281 2315 90-91 -14.482856976953062 2315 92-93 -13.998934306507026 2315 94-95 -13.489001073295524 2315 96-97 -12.88119650044679 2315 98-99 -12.197126111198196 2315 100-101 -11.010328195884297 2316 1 -18.667717282636833 2316 2 -17.990577698789426 2316 3 -18.737990992258418 2316 4 -20.31838404787378 2316 5 -19.454799494001197 2316 6 -20.12623254855231 2316 7 -19.907267864122677 2316 8 -19.696063191786873 2316 9 -18.65106205867704 2316 10-11 -18.6498884235571 2316 12-13 -18.322239855328775 2316 14-15 -19.83785318417222 2316 16-17 -21.00669015636962 2316 18-19 -21.066026793286138 2316 20-21 -21.313497442939777 2316 22-23 -21.26847518936763 2316 24-25 -21.305717490568956 2316 26-27 -21.17202409282316 2316 28-29 -21.115508566321722 2316 30-31 -21.289699000028133 2316 32-33 -21.25132649088049 2316 34-35 -21.002864002830847 2316 36-37 -20.50597767511543 2316 38-39 -20.542169367856776 2316 40-41 -20.545534020782227 2316 42-43 -20.492093779208275 2316 44-45 -20.168609253650846 2316 46-47 -19.87618708794899 2316 48-49 -19.779062584155927 2316 50-51 -17.03943702425013 2316 52-53 -19.234472790353834 2316 54-55 -20.18639203180324 2316 56-57 -20.241091171278466 2316 58-59 -20.003875758198195 2316 60-61 -19.75395846417076 2316 62-63 -19.35278177550191 2316 64-65 -19.066507258234676 2316 66-67 -18.792433489331145 2316 68-69 -18.39985167483861 2316 70-71 -18.110486944585357 2316 72-73 -17.751537280674683 2316 74-75 -17.362235779019024 2316 76-77 -17.02151682894055 2316 78-79 -16.736599594539435 2316 80-81 -16.380619396784276 2316 82-83 -15.992676216652644 2316 84-85 -15.683352582501783 2316 86-87 -15.244064001353518 2316 88-89 -14.892973996759281 2316 90-91 -14.482856976953062 2316 92-93 -13.998934306507026 2316 94-95 -13.489001073295524 2316 96-97 -12.88119650044679 2316 98-99 -12.197126111198196 2316 100-101 -11.010328195884297 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 902037.0 3 13500.0 4 47277.0 5 24517.0 6 20708.0 7 21715.0 8 17073.0 9 16895.0 10 18854.0 11 21232.0 12 23785.0 13 25525.0 14 27170.0 15 29630.0 16 32206.0 17 31592.0 18 31059.0 19 32949.0 20 35374.0 21 39161.0 22 43400.0 23 48108.0 24 53303.0 25 60377.0 26 66887.0 27 75646.0 28 85283.0 29 97327.0 30 110911.0 31 128014.0 32 148558.0 33 171015.0 34 194927.0 35 209398.0 36 183019.0 37 96955.0 38 15180.0 39 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.53613108360046 21.38510990853239 14.700377565085839 23.378381442781315 2 14.299605072861867 20.66605207749898 41.76865898966186 23.265683859977294 3 17.570824132002688 26.996017979512725 30.800135833555558 24.633022054929025 4 11.400117416269314 17.206316136145595 41.27512994544439 30.1184365021407 5 11.969685666845423 38.28336385316513 37.02925915864729 12.717691321342159 6 28.30628276438602 40.087781404350935 18.523164373563038 13.082771457700005 7 24.601108428773987 34.731833622782226 23.905808458991785 16.761249489451995 8 23.345422219982567 38.89532352207062 20.729711024167013 17.029543233779805 9 24.42371610832806 19.458395296472712 21.3396424801012 34.77824611509803 10-11 22.660538724970227 30.258970786872773 28.494519912836147 18.58597057532085 12-13 23.283186616066683 27.598112192157426 28.79187850497018 20.326822686805716 14-15 21.186995358003795 27.940468039381056 26.567929967469784 24.30460663514537 16-17 20.72734287581935 30.50475267573514 27.54487682525848 21.22302762318703 18-19 20.75351879242435 29.097576765194873 29.551668240383094 20.59723620199768 20-21 21.3884107527591 28.21257709480823 29.50118243981056 20.89782971262211 22-23 21.315398472701045 27.897777234950944 29.28844342607766 21.49838086627035 24-25 21.257544145291952 28.197702170943344 29.40812849651715 21.136625187247553 26-27 21.09617643567479 28.258937034589426 29.380418163037874 21.26446836669791 28-29 21.03832786907501 28.237318508562936 29.479885759667663 21.24446786269439 30-31 21.38680144464749 28.030815776066564 29.241080670234847 21.3413021090511 32-33 21.31330318343726 28.238486307972256 29.102496473252565 21.345714035337924 34-35 21.36752458295504 28.120250402540144 29.38010072281663 21.132124291688196 36-37 21.51479197105343 27.85680121242628 29.305262745021132 21.323144071499158 38-39 21.395704131496142 28.188735372290186 29.312983800576387 21.10257669563729 40-41 21.40045365298687 27.997851073353687 29.418814162649294 21.18288111101015 42-43 21.288941971884473 28.15324861151611 29.002889707926734 21.554919708672685 44-45 21.21872917550288 28.401523567555554 29.029905694814484 21.349841562127082 46-47 21.426067193486297 28.12548784305996 28.949904459070897 21.49854050438285 48-49 21.43747863031134 28.323937486810095 28.66540261720405 21.573181265674513 50-51 21.71931323501894 28.440665369773445 28.19081188810942 21.649209507098195 52-53 21.235950942409257 28.612551957972833 28.4429407449017 21.70855635471621 54-55 21.15628381003862 28.585496833511463 28.48669039718914 21.771528959260777 56-57 21.213786677314882 28.59331305860063 28.54748458097911 21.64541568310538 58-59 21.18667927954217 28.415477162119757 28.691061095482244 21.706782462855827 60-61 21.257810874901196 28.664683612763657 28.583413071514745 21.494092440820403 62-63 21.16330582738238 28.398289545442097 28.577150166554745 21.86125446062078 64-65 20.946121628863388 28.372642152416738 28.61061909813309 22.070617120586785 66-67 20.970291716337506 28.245236719962968 28.503647990430427 22.2808235732691 68-69 20.93963761266082 28.315442622004632 28.536679401207376 22.20824036412717 70-71 21.120881423443528 28.024354693191285 28.70206288645428 22.15270099691091 72-73 21.21223075568647 28.204718387217902 28.52068018504419 22.062370672051436 74-75 21.00879024946065 28.54405664879674 28.247659028911354 22.199494072831257 76-77 20.943826094293392 28.372492220132163 28.491463592115917 22.192218093458532 78-79 20.77487272895618 28.38084413148388 28.538346538020082 22.305936601539862 80-81 20.946099691572336 28.316264871992104 28.35657547194044 22.38105996449512 82-83 21.068126789028785 28.139221813275373 28.687729418738677 22.104921978957165 84-85 21.081993622220335 28.252357073894817 28.34880605322993 22.316843250654916 86-87 20.88048737683957 28.334184069997743 28.571654925163585 22.213673627999096 88-89 21.068590171915027 28.1851420560179 28.371155798314078 22.375111973752997 90-91 20.899241043093628 28.019198253785664 28.713802799508116 22.367757903612592 92-93 21.053816211505627 28.010397381774276 28.42726673084358 22.508519675876514 94-95 20.672991566031122 27.99018349439177 28.55044240185721 22.786382537719895 96-97 20.739149929366718 27.801307023175564 28.532664550563652 22.92687849689407 98-99 20.646201726283746 27.879044669800574 28.251816100719 23.22293750319668 100-101 20.77129926290296 27.829039553590174 28.22765650474436 23.172004678762505 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 200327.0 1 181732.0 2 120769.0 3 61345.5 4 37675.5 5 29007.5 6 26870.0 7 27738.5 8 28972.0 9 28316.0 10 25685.5 11 21557.5 12 16835.5 13 12667.0 14 9562.0 15 7837.0 16 7420.0 17 7998.5 18 9113.0 19 10601.0 20 12262.0 21 14034.5 22 15835.5 23 17474.5 24 19448.5 25 21908.0 26 25075.0 27 28741.5 28 32747.5 29 38715.0 30 45610.0 31 52422.0 32 60426.0 33 67568.5 34 73503.0 35 78589.5 36 83774.0 37 89259.5 38 90641.5 39 89031.5 40 87151.5 41 85687.5 42 85431.0 43 86451.5 44 88117.0 45 90276.0 46 90161.5 47 88253.5 48 87242.0 49 85875.5 50 83415.5 51 79054.0 52 73085.5 53 66957.5 54 61020.0 55 53153.5 56 43703.0 57 34882.0 58 27919.5 59 21856.0 60 16233.5 61 11939.0 62 8638.5 63 5882.5 64 3704.0 65 2236.0 66 1342.5 67 798.5 68 469.5 69 279.0 70 174.0 71 110.5 72 77.0 73 58.0 74 34.5 75 23.5 76 19.0 77 13.5 78 5.0 79 2.0 80 3.0 81 1.5 82 0.5 83 0.5 84 1.5 85 1.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.84419827430259 2 30.04936143197205 3 23.727917562440695 4 30.177771765160934 5 33.407807160797546 6 25.77589053815802 7 24.729174484023222 8 20.63947721060263 9 12.22275787278754 10-11 15.966309627619408 12-13 16.02153231956864 14-15 20.259292018532328 16-17 23.717013619306165 18-19 27.800337241152416 20-21 29.900267974556634 22-23 29.524863021167086 24-25 28.001607159985216 26-27 29.462298132551336 28-29 26.355080503281027 30-31 28.310822991510857 32-33 27.267762414014285 34-35 25.256187987728847 36-37 28.309776337943504 38-39 28.46455609235171 40-41 25.38550437922977 42-43 26.28409552104133 44-45 24.937161731721133 46-47 28.350845774190898 48-49 23.608286621861563 50-51 29.204727624811877 52-53 22.771666744254418 54-55 23.936154757259168 56-57 26.098712866059277 58-59 25.37314761845697 60-61 24.900263288048123 62-63 24.721613583626215 64-65 23.216525753770217 66-67 23.73269780112145 68-69 24.757184183221238 70-71 23.20866804116754 72-73 23.07857056491486 74-75 20.262307005674113 76-77 22.608045110456317 78-79 21.714187342240407 80-81 22.764636981488604 82-83 23.655542249342872 84-85 20.52206455071849 86-87 22.112181266650772 88-89 20.931509177589618 90-91 22.0764544501054 92-93 22.058161445219245 94-95 21.074400822076083 96-97 19.97100925835378 98-99 19.36693393303979 100-101 21.0561859256651 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3200677.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.47146105437575 #Duplication Level Percentage of deduplicated Percentage of total 1 79.46281756506356 48.052326776567064 2 11.808972168018306 14.282116011010523 3 3.902667898370245 7.080000894733761 4 1.70288690991396 4.119042378114733 5 0.9098108117086677 2.7508794533545347 6 0.5370395011165163 1.948533796585728 7 0.33882534245089924 1.4342484450178554 8 0.24111540657137093 1.1664480734472504 9 0.19269754809441336 1.0487432047378513 >10 0.8361100631742144 8.814566063686403 >50 0.046272052345426064 1.9102512306567117 >100 0.02006658178723297 2.045328216210511 >500 3.3145448549262696E-4 0.13923577042922927 >1k 3.3145448543505713E-4 0.29116578092581535 >5k 0.0 0.0 >10k+ 5.524241423917619E-5 4.917113904521974 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 147193 4.598808314615939 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.124339007028826E-5 0.0 0.0 0.0 5 0.0 3.124339007028826E-5 0.0 0.0 0.0 6 0.0 3.124339007028826E-5 0.0 0.0 0.0 7 0.0 3.124339007028826E-5 0.0 0.0 0.0 8 0.0 3.124339007028826E-5 0.0 0.0 0.0 9 0.0 3.124339007028826E-5 0.0 0.0 0.0 10-11 0.0 3.124339007028826E-5 0.0 7.810847517572065E-5 0.0 12-13 0.0 3.124339007028826E-5 0.0 9.373017021086477E-5 0.0 14-15 0.0 3.124339007028826E-5 0.0 9.373017021086477E-5 0.0 16-17 0.0 3.124339007028826E-5 0.0 9.373017021086477E-5 0.0 18-19 0.0 3.124339007028826E-5 0.0 9.373017021086477E-5 0.0 20-21 0.0 3.124339007028826E-5 0.0 9.373017021086477E-5 0.0 22-23 0.0 3.124339007028826E-5 0.0 9.373017021086477E-5 0.0 24-25 0.0 3.124339007028826E-5 0.0 1.4059525531629716E-4 0.0 26-27 0.0 3.124339007028826E-5 0.0 2.4994712056230605E-4 0.0 28-29 0.0 3.124339007028826E-5 0.0 7.654630567220622E-4 0.0 30-31 0.0 3.124339007028826E-5 0.0 0.0029837437517125282 0.0 32-33 0.0 3.124339007028826E-5 0.0 0.008748149219680711 0.0 34-35 0.0 3.124339007028826E-5 0.0 0.016746457077674505 0.0 36-37 0.0 3.124339007028826E-5 0.0 0.0324618822830295 0.0 38-39 0.0 3.124339007028826E-5 0.0 0.05545701737476165 0.0 40-41 4.6865085105432385E-5 3.124339007028826E-5 0.0 0.08006118705511366 0.0 42-43 6.248678014057651E-5 3.124339007028826E-5 0.0 0.10411859740923561 0.0 44-45 6.248678014057651E-5 3.124339007028826E-5 0.0 0.1328312728838305 0.0 46-47 6.248678014057651E-5 3.124339007028826E-5 0.0 0.17110442571993362 0.0 48-49 6.248678014057651E-5 3.124339007028826E-5 0.0 0.2126268911233467 0.0 50-51 6.248678014057651E-5 3.124339007028826E-5 0.0 0.2596325714840954 0.0 52-53 6.248678014057651E-5 3.124339007028826E-5 0.0 0.314324125802135 0.0 54-55 6.248678014057651E-5 3.124339007028826E-5 0.0 0.3777638293398553 0.0 56-57 6.248678014057651E-5 3.124339007028826E-5 0.0 0.4517325553312628 0.0 58-59 9.373017021086477E-5 3.124339007028826E-5 0.0 0.535871004790549 0.0 60-61 9.373017021086477E-5 3.124339007028826E-5 0.0 0.6283514393986023 0.0 62-63 9.373017021086477E-5 3.124339007028826E-5 0.0 0.7228470726661891 0.0 64-65 9.373017021086477E-5 3.124339007028826E-5 0.0 0.8257627995577186 0.0 66-67 9.373017021086477E-5 3.124339007028826E-5 0.0 0.94189448044898 0.0 68-69 9.373017021086477E-5 3.124339007028826E-5 0.0 1.075006943843443 0.0 70-71 9.373017021086477E-5 3.124339007028826E-5 0.0 1.217429937478852 0.0 72-73 9.373017021086477E-5 3.124339007028826E-5 0.0 1.3694758952559098 0.0 74-75 9.373017021086477E-5 3.124339007028826E-5 0.0 1.5218186652386354 0.0 76-77 9.373017021086477E-5 3.124339007028826E-5 0.0 1.7022804862846204 0.0 78-79 9.373017021086477E-5 3.124339007028826E-5 0.0 1.9000667671245801 0.0 80-81 9.373017021086477E-5 3.124339007028826E-5 0.0 2.1182081165953326 0.0 82-83 9.373017021086477E-5 3.124339007028826E-5 0.0 2.3444727474843603 0.0 84-85 1.0935186524600889E-4 3.124339007028826E-5 0.0 2.572268304486832 0.0 86-87 1.2497356028115303E-4 3.124339007028826E-5 0.0 2.814748254822339 0.0 88-89 1.2497356028115303E-4 3.124339007028826E-5 0.0 3.084628658249489 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2155 0.0 59.58093 1 GTATCAA 4550 0.0 47.746716 1 ATCAACG 5140 0.0 39.871353 3 CAACGCA 5190 0.0 39.475964 5 TCAACGC 5475 0.0 37.4255 4 TATCAAC 5720 0.0 36.450417 2 AACGCAG 5755 0.0 34.4525 6 GTACATG 5440 0.0 29.833399 1 ACGCAGA 6620 0.0 29.340023 7 CGCAGAG 6800 0.0 28.487753 8 TACATGG 5805 0.0 27.15868 2 ACATGGG 5800 0.0 26.718275 3 GCAGAGT 7365 0.0 24.429213 9 GAGTACA 3820 0.0 22.99044 1 GAGTACT 4565 0.0 21.942629 12-13 TATCGGC 165 3.468249E-5 21.786455 2 AGAGTAC 6480 0.0 21.309849 10-11 CAGAGTA 6915 0.0 20.63055 10-11 CATGGGG 3845 0.0 20.213898 4 AGTACAT 4240 0.0 20.105492 2 >>END_MODULE