FastQCFastQC Report
Sat 18 Jun 2016
SRR1930992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3200677
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62300.19464632013789582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51370.16049729479107078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.10160350450857741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32850.059.011821
GTATCAA68600.044.327181
CAACGCA85950.034.554495
ATCAACG87250.034.3026963
TCAACGC89300.033.3545884
TATCAAC91100.032.750262
AACGCAG92050.032.625416
ACGCAGA104900.028.312287
CGCAGAG107450.027.5520698
GTACATG76700.024.3450931
GCAGAGT123950.023.3102449
ACGTGCG1652.457451E-623.0028729
CTAACGC2103.4460754E-822.6221473
TACATGG85100.021.8283392
ACATGGG81400.021.4187813
AGAGTAC110450.021.26255610-11
GAGTACT80200.020.76389312-13
GGCGTCG4800.020.7564988
GAGTACA58200.020.327811
CAGAGTA117150.020.04651810-11
Produced by FastQC (version 0.11.5)