##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930992_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3200677 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.320286301929247 31.0 31.0 34.0 30.0 34.0 2 31.481389718487684 31.0 31.0 34.0 30.0 34.0 3 31.552188490122557 31.0 31.0 34.0 30.0 34.0 4 35.33600360173801 37.0 35.0 37.0 33.0 37.0 5 35.20218160095505 37.0 35.0 37.0 33.0 37.0 6 35.21838848468621 37.0 35.0 37.0 32.0 37.0 7 35.089551679222865 37.0 35.0 37.0 32.0 37.0 8 35.092876288360245 37.0 35.0 37.0 32.0 37.0 9 36.586430308337896 39.0 35.0 39.0 32.0 39.0 10-11 36.52103086315802 38.0 35.0 39.0 32.0 39.0 12-13 36.409484774627366 38.0 35.0 39.0 32.0 39.0 14-15 37.47009945083494 39.0 36.0 41.0 32.0 41.0 16-17 37.42112731150316 39.0 36.0 41.0 32.0 41.0 18-19 37.43955044510896 39.0 36.0 41.0 32.0 41.0 20-21 37.41262286072603 39.0 36.0 41.0 32.0 41.0 22-23 37.3099292743379 39.0 36.0 40.5 31.5 41.0 24-25 37.189930286623735 39.0 36.0 40.0 31.0 41.0 26-27 36.935754685649314 39.0 36.0 40.0 31.0 41.0 28-29 36.75832409830795 39.0 35.5 40.0 30.0 41.0 30-31 36.54406958277889 38.0 35.0 40.0 30.0 41.0 32-33 36.390057166030815 38.0 35.0 40.0 30.0 41.0 34-35 36.48649988736758 38.0 35.0 40.0 30.0 41.0 36-37 36.567888606066774 38.5 35.0 40.0 30.0 41.0 38-39 36.562518804615394 39.0 35.0 40.0 30.0 41.0 40-41 36.4540378488676 39.0 35.0 40.0 30.0 41.0 42-43 36.33084984832896 38.5 35.0 40.0 30.0 41.0 44-45 36.17628473601054 38.0 35.0 40.0 30.0 41.0 46-47 35.96045961526265 38.0 35.0 40.0 29.0 41.0 48-49 35.76263021854439 38.0 35.0 40.0 28.0 41.0 50-51 35.50459512159459 38.0 34.0 40.0 27.5 41.0 52-53 35.29409949832488 38.0 34.0 40.0 27.0 41.0 54-55 35.14514538642918 38.0 34.0 40.0 27.0 41.0 56-57 34.96978014338841 38.0 34.0 40.0 26.0 41.0 58-59 34.763701866823794 38.0 33.5 40.0 26.0 41.0 60-61 34.44877536846111 37.0 33.0 40.0 26.0 41.0 62-63 34.185606045221064 37.0 33.0 40.0 26.0 41.0 64-65 33.86103439990977 36.5 32.5 39.0 25.0 41.0 66-67 33.53596098575395 36.0 32.0 39.0 24.5 40.5 68-69 33.198539090323706 36.0 32.0 39.0 23.5 40.0 70-71 32.82872326698383 35.0 31.0 38.5 23.0 40.0 72-73 32.42013877064134 35.0 31.0 38.0 22.0 40.0 74-75 31.900438094815566 35.0 31.0 37.0 21.5 39.0 76-77 30.503968535406727 33.5 29.5 35.5 20.0 38.5 78-79 31.26390510507621 34.0 30.0 36.0 21.0 39.0 80-81 31.245430732310695 34.0 31.0 36.0 21.0 38.5 82-83 30.988668959723206 34.0 30.5 35.5 20.5 37.0 84-85 30.665899589368124 34.0 30.0 35.0 20.0 37.0 86-87 30.337696524828964 34.0 30.0 35.0 19.0 36.0 88-89 29.999042233877397 34.0 30.0 35.0 18.0 36.0 90-91 29.67419720890299 34.0 29.5 35.0 15.0 36.0 92-93 29.366427633903704 34.0 29.0 35.0 8.5 35.0 94-95 28.99523569544818 34.0 29.0 35.0 2.0 35.0 96-97 28.583164124339945 34.0 29.0 35.0 2.0 35.0 98-99 28.099548626743655 34.0 29.0 35.0 2.0 35.0 100-101 26.976283767465446 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2938672019781805 1101 2 0.3931765005118848 1101 3 0.3146147952960128 1101 4 0.24254239424679724 1101 5 0.334321395488935 1101 6 0.30395492476974084 1101 7 0.371123892922931 1101 8 0.3601900389352011 1101 9 0.4845557860225682 1101 10-11 0.40022212192267403 1101 12-13 0.35521365470076205 1101 14-15 0.41806086294956657 1101 16-17 0.5096238482519908 1101 18-19 0.48953109076942525 1101 20-21 0.4887133294282435 1101 22-23 0.48330403004280953 1101 24-25 0.42453070161552375 1101 26-27 0.4440501589131429 1101 28-29 0.5611854872634794 1101 30-31 0.4715361779006102 1101 32-33 0.4547424214074809 1101 34-35 0.48864833578517874 1101 36-37 0.5404252313228355 1101 38-39 0.5426344428534691 1101 40-41 0.6069776864648517 1101 42-43 0.5520602096002492 1101 44-45 0.5169999215368293 1101 46-47 0.5057211174052085 1101 48-49 0.6001232649629173 1101 50-51 0.7177763919859856 1101 52-53 0.6250945656953135 1101 54-55 0.5340908894137115 1101 56-57 0.6245451643341013 1101 58-59 0.5198772556667777 1101 60-61 0.5504952858513903 1101 62-63 0.3823117147486883 1101 64-65 0.48903791914562333 1101 66-67 0.40848257048474323 1101 68-69 0.37912596696102696 1101 70-71 0.5882763038724761 1101 72-73 0.47680827718058083 1101 74-75 0.3744863259158073 1101 76-77 0.4123239306028168 1101 78-79 0.501984583881427 1101 80-81 0.27005039982804746 1101 82-83 0.26437503448510924 1101 84-85 0.35372706091521167 1101 86-87 0.23956109408377912 1101 88-89 0.41277215045635884 1101 90-91 0.4193903277019828 1101 92-93 0.31035525869526737 1101 94-95 0.4611953350686342 1101 96-97 0.6161759695496123 1101 98-99 0.757441590473821 1101 100-101 0.8192620665578652 1102 1 0.2910132607378557 1102 2 0.3747883705756294 1102 3 0.35874163137135184 1102 4 0.24919828437305114 1102 5 0.36087208682505434 1102 6 0.32039198270943103 1102 7 0.3144373471421673 1102 8 0.3238528158051537 1102 9 0.44498975026121457 1102 10-11 0.36057238782429835 1102 12-13 0.4897108098681642 1102 14-15 0.5050987838626284 1102 16-17 0.5081040706486917 1102 18-19 0.6097141603872132 1102 20-21 0.5126972042908307 1102 22-23 0.4930247265668086 1102 24-25 0.5119788152250351 1102 26-27 0.4436576268694381 1102 28-29 0.48903979791000296 1102 30-31 0.5483739838828043 1102 32-33 0.6186376812882841 1102 34-35 0.5409786715642824 1102 36-37 0.5641574545348718 1102 38-39 0.4830160263045542 1102 40-41 0.5188824541528732 1102 42-43 0.38867068199284915 1102 44-45 0.46112333680952133 1102 46-47 0.399675020773806 1102 48-49 0.41875969659210455 1102 50-51 0.26360598824423676 1102 52-53 0.18488285849416286 1102 54-55 0.3158884139265936 1102 56-57 0.35446677330154586 1102 58-59 0.4484146135643101 1102 60-61 0.3517831370833093 1102 62-63 0.12532270119279332 1102 64-65 0.26668640475224237 1102 66-67 0.15412620867231652 1102 68-69 0.21570506512814092 1102 70-71 0.28985934608336095 1102 72-73 0.28167294192876824 1102 74-75 0.2860356033876279 1102 76-77 0.41662098500835754 1102 78-79 0.4458330192487985 1102 80-81 0.48055814410017916 1102 82-83 0.38007543573109004 1102 84-85 0.3405414860149172 1102 86-87 0.27079408495226076 1102 88-89 0.36777300970530646 1102 90-91 0.41517985603806906 1102 92-93 0.3371127162980585 1102 94-95 0.503715912006232 1102 96-97 0.6047232133643874 1102 98-99 0.7366784822718095 1102 100-101 0.8906099903229467 1103 1 0.27589181629608817 1103 2 0.33508166034062725 1103 3 0.3094233043993846 1103 4 0.15118536038934138 1103 5 0.21139549194026586 1103 6 0.251669488458262 1103 7 0.29485617940128606 1103 8 0.23146575316223306 1103 9 0.4157680557079644 1103 10-11 0.3219091936444656 1103 12-13 0.3938297581117567 1103 14-15 0.5185478689423277 1103 16-17 0.4618683542172022 1103 18-19 0.4761040835542687 1103 20-21 0.3933653613669392 1103 22-23 0.2936125155999534 1103 24-25 0.37957991321790274 1103 26-27 0.38723828720898723 1103 28-29 0.4261842297950622 1103 30-31 0.4513049640277771 1103 32-33 0.5534050461398223 1103 34-35 0.5374413665044813 1103 36-37 0.3819758451778412 1103 38-39 0.44410512981197314 1103 40-41 0.5889714903017804 1103 42-43 0.54273906218787 1103 44-45 0.5886175068568136 1103 46-47 0.695842124943006 1103 48-49 0.6049252913407059 1103 50-51 0.6114804831168854 1103 52-53 0.4590296302269934 1103 54-55 0.553671325810356 1103 56-57 0.653970877008085 1103 58-59 0.6766300113261394 1103 60-61 0.5052336873334937 1103 62-63 0.4470237444630172 1103 64-65 0.4152710648105611 1103 66-67 0.4635574324387619 1103 68-69 0.34767277434391985 1103 70-71 0.45649171071610795 1103 72-73 0.490537026715927 1103 74-75 0.3995450161246872 1103 76-77 0.5009103256963883 1103 78-79 0.5568465779758327 1103 80-81 0.626567693275085 1103 82-83 0.5384921764347581 1103 84-85 0.5256975389636196 1103 86-87 0.48931020158179805 1103 88-89 0.3741688722211798 1103 90-91 0.39006250719031854 1103 92-93 0.22062802413382343 1103 94-95 0.4488709422856054 1103 96-97 0.5974583769152702 1103 98-99 0.7867776271137465 1103 100-101 0.8010289749991308 1104 1 0.35768056216616273 1104 2 0.368834623752214 1104 3 0.3858003776092005 1104 4 0.206024146085781 1104 5 0.2927836175559264 1104 6 0.30502173735184357 1104 7 0.30397853825333954 1104 8 0.4023764808300072 1104 9 0.4873442843160163 1104 10-11 0.39304568260855177 1104 12-13 0.4059784494974821 1104 14-15 0.528279406240074 1104 16-17 0.4902518279247303 1104 18-19 0.5310965189012293 1104 20-21 0.44951912413376505 1104 22-23 0.4360250036402604 1104 24-25 0.49622757006073215 1104 26-27 0.5311917492749672 1104 28-29 0.5473343869795997 1104 30-31 0.4804590425776283 1104 32-33 0.5525342862339002 1104 34-35 0.5853642683148124 1104 36-37 0.5078724097413243 1104 38-39 0.6185693591364299 1104 40-41 0.5038432211226791 1104 42-43 0.5148424599189454 1104 44-45 0.6019397799290047 1104 46-47 0.4866985567833666 1104 48-49 0.40274780242845054 1104 50-51 0.39271260249228845 1104 52-53 0.3278561899359076 1104 54-55 0.4518958711063661 1104 56-57 0.6110118958089572 1104 58-59 0.634134571327273 1104 60-61 0.4851274994001784 1104 62-63 0.2906607165280022 1104 64-65 0.21295524408105138 1104 66-67 0.2887618665980014 1104 68-69 0.2750332524488428 1104 70-71 0.34699465409669017 1104 72-73 0.3479218719586754 1104 74-75 0.4189498829537932 1104 76-77 0.44678228746307624 1104 78-79 0.5362587031013213 1104 80-81 0.4560453315951172 1104 82-83 0.4834262154881621 1104 84-85 0.5274112510166376 1104 86-87 0.4674250240598923 1104 88-89 0.5166006842972912 1104 90-91 0.4397394187380783 1104 92-93 0.46895107488062493 1104 94-95 0.6293805312492218 1104 96-97 0.5421063259752295 1104 98-99 0.6006350817140458 1104 100-101 0.9607508502789806 1105 1 0.3003156146765953 1105 2 0.34404412598210854 1105 3 0.311167231299315 1105 4 0.22663898567720508 1105 5 0.3166425946815181 1105 6 0.3031784773892454 1105 7 0.31635625368996756 1105 8 0.37547297773692634 1105 9 0.3780758337333694 1105 10-11 0.45353922670292945 1105 12-13 0.49391588655689844 1105 14-15 0.5184777589950755 1105 16-17 0.5518616682354818 1105 18-19 0.4629489502060764 1105 20-21 0.5251530257306598 1105 22-23 0.4563597576324909 1105 24-25 0.544853667025194 1105 26-27 0.5580867104316312 1105 28-29 0.4799713277075526 1105 30-31 0.4639537735515873 1105 32-33 0.5902410910101921 1105 34-35 0.607379985477813 1105 36-37 0.5209532614173469 1105 38-39 0.4926917875639987 1105 40-41 0.46433271903664775 1105 42-43 0.6426473620913029 1105 44-45 0.5993601151325763 1105 46-47 0.6232270239103954 1105 48-49 0.5794373075356347 1105 50-51 0.6048156272369312 1105 52-53 0.49090736962228476 1105 54-55 0.5700236584750868 1105 56-57 0.6125377553975042 1105 58-59 0.7014463789808403 1105 60-61 0.6614398105784218 1105 62-63 0.5434606733687488 1105 64-65 0.5480711446708924 1105 66-67 0.6381567378395445 1105 68-69 0.5310126079372566 1105 70-71 0.5039833068085215 1105 72-73 0.6495259577017336 1105 74-75 0.7274805799758077 1105 76-77 0.7029216918653205 1105 78-79 0.7062014271697983 1105 80-81 0.6503091965066403 1105 82-83 0.6345973599278132 1105 84-85 0.7684107474919628 1105 86-87 0.7016381768436659 1105 88-89 0.71252528147755 1105 90-91 0.7411870521721191 1105 92-93 0.762258242914001 1105 94-95 0.8991910800911178 1105 96-97 0.9040389615672275 1105 98-99 0.8605221483231631 1105 100-101 0.9850292814399602 1106 1 0.2510588702875296 1106 2 0.2514761540860775 1106 3 0.24170072796232134 1106 4 0.15607229507429565 1106 5 0.20355809269621972 1106 6 0.20339003155556412 1106 7 0.30517556319969685 1106 8 0.2604102154942396 1106 9 0.4017368120217881 1106 10-11 0.2393945778617237 1106 12-13 0.3707497373355295 1106 14-15 0.3493366829904545 1106 16-17 0.3792207963929499 1106 18-19 0.3852995950766811 1106 20-21 0.3669546857796533 1106 22-23 0.3070976451884633 1106 24-25 0.26511358648676975 1106 26-27 0.30025401422481934 1106 28-29 0.3928135632120515 1106 30-31 0.36023459146716874 1106 32-33 0.4671116381645959 1106 34-35 0.41236629253759105 1106 36-37 0.41448695520237067 1106 38-39 0.4196911680376445 1106 40-41 0.3886783443358439 1106 42-43 0.4230894258180271 1106 44-45 0.5411410069689069 1106 46-47 0.5223932153721975 1106 48-49 0.4646976846018873 1106 50-51 0.3950601113404488 1106 52-53 0.4330149208036076 1106 54-55 0.5916562159924794 1106 56-57 0.6214207423592981 1106 58-59 0.6259209256002052 1106 60-61 0.6025046390822624 1106 62-63 0.4144298800934152 1106 64-65 0.4179300440260292 1106 66-67 0.4520357119602423 1106 68-69 0.4028069111109218 1106 70-71 0.5025280359065505 1106 72-73 0.6653083913138573 1106 74-75 0.7966775365526466 1106 76-77 0.5105862183500705 1106 78-79 0.6154754021353028 1106 80-81 0.5132382008996466 1106 82-83 0.6012755547125259 1106 84-85 0.5255638127393496 1106 86-87 0.6921786959785798 1106 88-89 0.6324438454436461 1106 90-91 0.6508879195089996 1106 92-93 0.7428296679653954 1106 94-95 0.817568396274833 1106 96-97 0.7693372142213342 1106 98-99 0.838126974996591 1106 100-101 1.1074713592468406 1107 1 0.17846741887653295 1107 2 0.18218557821517578 1107 3 0.1493585948012388 1107 4 0.008927879495601587 1107 5 0.08182116611008894 1107 6 0.14051580362409055 1107 7 0.22365808746695848 1107 8 0.19899227676088316 1107 9 0.14440975860766514 1107 10-11 0.1576883319972211 1107 12-13 0.1920349711472582 1107 14-15 0.2438382292701391 1107 16-17 0.19164279807872475 1107 18-19 0.2611687816168953 1107 20-21 0.24517853593474115 1107 22-23 0.23422410989513764 1107 24-25 0.2648033860432335 1107 26-27 0.23333349392764546 1107 28-29 0.28061370422337717 1107 30-31 0.3843129498307931 1107 32-33 0.3765617062127973 1107 34-35 0.2742111744751483 1107 36-37 0.39865956132861413 1107 38-39 0.4239769103087383 1107 40-41 0.23394599913129355 1107 42-43 0.2387533588378261 1107 44-45 0.23553099652860965 1107 46-47 0.26625331051199197 1107 48-49 0.23983179141613675 1107 50-51 0.22925089047266312 1107 52-53 0.2806127082794845 1107 54-55 0.37640675519204336 1107 56-57 0.21698355545665748 1107 58-59 0.282945011042365 1107 60-61 0.35969753758227085 1107 62-63 0.14102426402239843 1107 64-65 0.03972394709621341 1107 66-67 0.22355122172005792 1107 68-69 0.07867971825965014 1107 70-71 0.06607015241633007 1107 72-73 0.09331477757751117 1107 74-75 0.2608018976333035 1107 76-77 0.25607904176180085 1107 78-79 0.3804499852886991 1107 80-81 0.38657527902660505 1107 82-83 0.4133397808092063 1107 84-85 0.31680988975756463 1107 86-87 0.3371167704550224 1107 88-89 0.5271908759762205 1107 90-91 0.47839867595206087 1107 92-93 0.44472367473890273 1107 94-95 0.5507263137497951 1107 96-97 0.5847306947822553 1107 98-99 0.6494085724580358 1107 100-101 0.8868547398842104 1108 1 0.12745855821104612 1108 2 0.18938632309317427 1108 3 0.11460388898125728 1108 4 0.019120757050487214 1108 5 0.11657132161496264 1108 6 0.1942170771542635 1108 7 0.21300339585597783 1108 8 0.16467877979650325 1108 9 0.19315462286876084 1108 10-11 0.24597672238335377 1108 12-13 0.14194896589894057 1108 14-15 0.11435472008430025 1108 16-17 0.16272627532649864 1108 18-19 0.13515029445930082 1108 20-21 0.0857312446815186 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-3.0336851977719625 2316 76-77 -1.9509332905813466 2316 78-79 -2.081926723428815 2316 80-81 -1.7078539482408601 2316 82-83 -1.290708416919788 2316 84-85 -1.25568345540659 2316 86-87 -1.1149066854301672 2316 88-89 -1.2401586347165257 2316 90-91 -1.2996525823584442 2316 92-93 -1.2386900310080442 2316 94-95 -1.7625074863865144 2316 96-97 -1.5537143240570401 2316 98-99 -1.825747993003688 2316 100-101 -2.308532526553556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 11.0 4 45.0 5 74.0 6 150.0 7 416.0 8 1023.0 9 1930.0 10 3344.0 11 5266.0 12 7391.0 13 9706.0 14 11723.0 15 15033.0 16 18262.0 17 21102.0 18 18259.0 19 14838.0 20 14849.0 21 15718.0 22 17902.0 23 20819.0 24 26067.0 25 33295.0 26 43245.0 27 56226.0 28 71981.0 29 89885.0 30 110867.0 31 134086.0 32 163005.0 33 198150.0 34 244609.0 35 305329.0 36 400370.0 37 538758.0 38 494561.0 39 92367.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.489374784997686 22.576734539439663 14.980657993774187 22.953232681788457 2 14.896207075297637 22.155697042205507 40.557994962597554 22.390100919899304 3 17.733171031040314 27.36029859289411 31.363439242244922 23.543091133820656 4 12.245017392379275 18.411010948670967 39.99264559760778 29.351326061341975 5 12.075816560541396 38.996038267585654 36.456061638057626 12.472083533815322 6 28.792387324872166 40.44588275800444 17.975874990861264 12.78585492626213 7 25.177735835262354 33.6668461078703 24.024073656916958 17.131344399950386 8 24.099249405748306 37.59532397027617 20.952464863540815 17.35296176043471 9 24.959844432912163 16.53228363874268 21.927548453030408 36.58032347531475 10-11 23.501731040026844 27.694281553558824 29.563620446549276 19.240366959865053 12-13 24.053817364263875 25.08531788743444 29.829548561132533 21.031316187169153 14-15 21.467926941706395 26.77405436412359 27.0556979039122 24.70232079025781 16-17 20.790257811081844 29.9807978124628 27.915687837291927 21.313256539163433 18-19 20.681608922112417 28.900307653662022 30.07766794337573 20.34041548084983 20-21 21.24400556507264 28.10902193504687 29.957662082115753 20.68931041776474 22-23 21.25462325653473 27.83995580919721 29.81459171309971 21.090829221168345 24-25 21.130245541659633 28.216568559251165 29.778262816854717 20.874923082234485 26-27 20.98137981316231 28.281669088358992 29.887609626426165 20.849341472052537 28-29 20.983006375850564 28.234595183322497 29.866128071270055 20.916270369556884 30-31 21.342948625313724 28.08031351767677 29.634372585794377 20.94236527121513 32-33 21.2891569521847 28.259802646289106 29.43844677038885 21.012593631137342 34-35 21.3392564474252 28.197792596846917 29.74229047840609 20.720660477321793 36-37 21.419277556008552 27.95682563804628 29.800748254377744 20.823148551567424 38-39 21.22152976239015 28.20219972899399 29.778543034936156 20.797727473679707 40-41 21.196823056507583 28.067234399797414 29.838076862211377 20.897865681483623 42-43 21.169809811461306 28.213763218300297 29.40774577867436 21.208681191564036 44-45 21.07479714095249 28.393580063219503 29.4749013142426 21.056721481585402 46-47 21.327076267326934 28.204339023322138 29.284790608214124 21.183794101136804 48-49 21.315897850598002 28.396366376988386 28.981571286978973 21.306164485434635 50-51 21.395597004985483 28.523946295838055 28.67969468869964 21.40076201047682 52-53 21.320770326848884 28.639341444643566 28.677204373958126 21.362683854549424 54-55 21.252432724313746 28.63288393295743 28.741348751107772 21.373334591621056 56-57 21.272237344991613 28.61056119222415 28.715326989269087 21.401874473515157 58-59 21.197452137741557 28.521704593411222 28.939614127020313 21.341229141826908 60-61 21.354346615298688 28.782328973300984 28.75162642933793 21.111697982062395 62-63 21.264223742358197 28.527075510415305 28.803192776168547 21.40550797105795 64-65 20.993643044649016 28.468186097378105 28.815676481144294 21.72249437682859 66-67 20.990258001349297 28.36655522752315 28.86514986654238 21.778036904585164 68-69 21.019693278020878 28.504852106624984 28.76984701797728 21.70560759737686 70-71 21.16631775414115 28.213797520085304 28.92166457110378 21.698220154669766 72-73 21.28469750332874 28.40024971406488 28.738271183841814 21.576781598764573 74-75 21.160437732907482 28.673538827678385 28.588929222443838 21.5770942169703 76-77 20.987775312223782 28.592033334763194 28.711891196114454 21.708300156898574 78-79 20.876565116305954 28.61691726962291 28.729853418907968 21.776664195163164 80-81 21.01165503787325 28.55184342307687 28.604754120692004 21.83174741835787 82-83 21.119032004791485 28.4690863839119 28.834883994854838 21.57699761644177 84-85 21.166803148208956 28.548350864520224 28.470304876124647 21.814541111146173 86-87 20.93828899323487 28.616227129447925 28.638597396738252 21.806886480578953 88-89 21.068296030542292 28.46413875316671 28.459280405251832 22.008284811039168 90-91 20.944312537435707 28.259761717513616 28.812146401469523 21.983779343581148 92-93 21.108717251460938 28.390491441556797 28.57384591755439 21.92694538942788 94-95 20.722092089924697 28.28054461225731 28.64491504532447 22.352448252493527 96-97 20.818345618755032 28.155543342861527 28.606416704965852 22.419694333417585 98-99 20.701464096502082 28.42042480387743 28.43540600941613 22.442705090204353 100-101 20.698511596140442 28.2881715337099 28.396586097253802 22.61673077289586 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 124.0 1 104.5 2 115.0 3 164.5 4 154.0 5 156.5 6 269.5 7 576.5 8 1014.0 9 1257.0 10 1223.5 11 1192.0 12 1261.5 13 1433.0 14 1717.0 15 2021.0 16 2301.5 17 2589.0 18 3002.0 19 3439.0 20 3970.5 21 4625.5 22 5550.5 23 6960.0 24 8879.5 25 11829.5 26 16310.0 27 21835.5 28 28073.5 29 37508.5 30 48677.5 31 59817.5 32 73716.0 33 85220.0 34 94854.0 35 103219.5 36 112965.0 37 124314.5 38 126246.5 39 123266.0 40 119864.0 41 116729.0 42 116645.5 43 119711.5 44 123905.5 45 128700.5 46 128991.5 47 125638.5 48 125039.5 49 124059.5 50 121372.0 51 115623.5 52 106199.5 53 97444.5 54 89416.0 55 77324.5 56 62791.0 57 49543.0 58 38535.0 59 29288.5 60 20931.0 61 14557.0 62 10469.5 63 6824.0 64 3917.0 65 2256.0 66 1235.0 67 701.5 68 405.5 69 228.0 70 144.5 71 106.0 72 59.0 73 35.0 74 29.0 75 23.5 76 16.5 77 8.5 78 4.5 79 3.0 80 2.0 81 1.5 82 1.0 83 0.5 84 1.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004061640709137473 2 0.006811059035322839 3 0.0020308203545687364 4 0.1659648880533712 5 0.0017808732340064306 6 4.6865085105432384E-4 7 0.0 8 4.061640709137473E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.436772907731708E-4 24-25 5.780027163003327E-4 26-27 0.002858770191431375 28-29 1.8746034042172954E-4 30-31 0.006857924120428273 32-33 0.021448587283252888 34-35 0.013934551971348562 36-37 8.591932269329269E-4 38-39 3.1243390070288254E-4 40-41 0.0016715213687604218 42-43 0.011841244836639248 44-45 0.007514035311904326 46-47 0.010888321439495457 48-49 0.010778969574249448 50-51 0.19063154451386377 52-53 0.11305420696933804 54-55 0.01752754182943171 56-57 0.006404894964409092 58-59 0.01819927471594291 60-61 0.12102127143726156 62-63 0.23334125873994785 64-65 0.23866825674693196 66-67 0.2469165117254881 68-69 0.24324541339222921 70-71 0.23188844110167942 72-73 0.15690430493298763 74-75 0.2799095316397125 76-77 0.21539193114456726 78-79 0.006123704453776498 80-81 3.124339007028826E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.562169503514413E-5 90-91 0.0059362441133547685 92-93 0.018480465226575503 94-95 0.0027494183261853665 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3200677.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.242779221981145 #Duplication Level Percentage of deduplicated Percentage of total 1 66.35671617723014 27.367353951931673 2 16.296753846998595 13.442468418934697 3 6.460804813537524 7.993846395631235 4 3.316110786592594 5.470625001882743 5 1.8880246979883892 3.8933692892391383 6 1.2314991130393804 3.0474267618689264 7 0.860343192254229 2.483806103329295 8 0.5911870345572269 1.9505757076113155 9 0.4527857236115367 1.6806727470398024 >10 2.2612139203673505 16.807410981854908 >50 0.16979573162774106 4.838226724025829 >100 0.10783541566751206 8.275415869670146 >500 0.005406569269183672 1.4350929397181917 >1k 0.0013706795326941304 0.9567181576507103 >5k 1.522977258549034E-4 0.3569909496115164 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6230 0.19464632013789582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5137 0.16049729479107078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3252 0.10160350450857741 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.124339007028826E-5 0.0 16-17 0.0 0.0 0.0 3.124339007028826E-5 0.0 18-19 0.0 0.0 0.0 3.124339007028826E-5 0.0 20-21 0.0 0.0 0.0 3.124339007028826E-5 0.0 22-23 0.0 0.0 0.0 4.6865085105432385E-5 0.0 24-25 0.0 0.0 0.0 1.4059525531629716E-4 0.0 26-27 0.0 0.0 0.0 3.5929898580831494E-4 0.0 28-29 0.0 0.0 0.0 0.0012653572978466743 0.0 30-31 0.0 0.0 0.0 0.005061429191386697 0.0 32-33 0.0 0.0 0.0 0.01505931401387894 0.0 34-35 0.0 0.0 0.0 0.029290678190895238 0.0 36-37 0.0 0.0 0.0 0.0550508533038479 0.0 38-39 0.0 0.0 0.0 0.09547980005480092 0.0 40-41 0.0 0.0 0.0 0.1433446736424825 0.0 42-43 0.0 0.0 0.0 0.1917719282514293 0.0 44-45 0.0 0.0 0.0 0.2515405334558907 0.0 46-47 0.0 0.0 0.0 0.328742950319573 0.0 48-49 0.0 0.0 0.0 0.40966333060161964 0.0 50-51 0.0 0.0 0.0 0.4972541746636727 0.0 52-53 0.0 0.0 0.0 0.5865009183994512 0.0 54-55 0.0 0.0 0.0 0.6911662751349168 0.0 56-57 0.0 0.0 0.0 0.8142808537068876 0.0 58-59 0.0 0.0 0.0 0.9537669686756896 0.0 60-61 0.0 0.0 0.0 1.1083280193534055 0.0 62-63 0.0 0.0 0.0 1.2634670727474218 0.0 64-65 0.0 0.0 0.0 1.4303223974177963 0.0 66-67 0.0 0.0 0.0 1.6228597887259477 0.0 68-69 0.0 0.0 0.0 1.8414541673527194 0.0 70-71 0.0 0.0 0.0 2.0774511142486416 0.0 72-73 0.0 0.0 0.0 2.3309599812789603 0.0 74-75 0.0 0.0 0.0 2.5851249595007557 0.0 76-77 0.0 0.0 0.0 2.87718816987781 0.0 78-79 0.0 1.562169503514413E-5 0.0 3.2048688449349934 0.0 80-81 0.0 3.124339007028826E-5 0.0 3.5666985453390017 0.0 82-83 0.0 3.124339007028826E-5 0.0 3.9417910648278474 0.0 84-85 0.0 6.248678014057651E-5 0.0 4.3197267328130895 0.0 86-87 0.0 6.248678014057651E-5 0.0 4.723094520315545 0.0 88-89 0.0 6.248678014057651E-5 0.0 5.1751863746326165 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3285 0.0 59.01182 1 GTATCAA 6860 0.0 44.32718 1 CAACGCA 8595 0.0 34.55449 5 ATCAACG 8725 0.0 34.302696 3 TCAACGC 8930 0.0 33.354588 4 TATCAAC 9110 0.0 32.75026 2 AACGCAG 9205 0.0 32.62541 6 ACGCAGA 10490 0.0 28.31228 7 CGCAGAG 10745 0.0 27.552069 8 GTACATG 7670 0.0 24.345093 1 GCAGAGT 12395 0.0 23.310244 9 ACGTGCG 165 2.457451E-6 23.002872 9 CTAACGC 210 3.4460754E-8 22.622147 3 TACATGG 8510 0.0 21.828339 2 ACATGGG 8140 0.0 21.418781 3 AGAGTAC 11045 0.0 21.262556 10-11 GAGTACT 8020 0.0 20.763893 12-13 GGCGTCG 480 0.0 20.756498 8 GAGTACA 5820 0.0 20.32781 1 CAGAGTA 11715 0.0 20.046518 10-11 >>END_MODULE