FastQCFastQC Report
Sat 18 Jun 2016
SRR1930991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394827
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN619254.439618676724784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10450.063.2352681
TTACGCG206.889279E-445.19108680-81
GTATCAA19850.042.5803641
ATCAACG22150.034.1094063
TCAACGC22450.033.65364
CAACGCA22850.033.2705845
TATCAAC24900.030.8519612
AACGCAG26300.030.246296
GCCGATA700.005809848729.2652932
ACGCAGA29600.026.879927
CGCAGAG29750.026.6918968
GCAGAGT33250.022.8939559
GTGTAGC5300.022.229921
GTGGTAT5400.021.8182561
GAGTACT22700.021.683912-13
GTACATG21900.021.5193751
ACATGGG20850.021.258533
TACATGG21300.020.91852
GAGTACA14450.020.5610891
TAGCCCG1250.00430718120.138625
Produced by FastQC (version 0.11.5)