##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930991_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1394827 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.176891471128677 28.0 2.0 31.0 2.0 31.0 2 20.452347853891556 30.0 2.0 31.0 2.0 33.0 3 21.251842701639703 30.0 2.0 31.0 2.0 33.0 4 22.866295963585447 32.0 2.0 35.0 2.0 37.0 5 22.019578772134466 32.0 2.0 35.0 2.0 37.0 6 22.727270120237133 32.0 2.0 35.0 2.0 37.0 7 22.516304172488773 30.0 2.0 35.0 2.0 37.0 8 22.324564264959022 30.0 2.0 35.0 2.0 37.0 9 21.2969852175216 27.0 2.0 34.0 2.0 37.0 10-11 21.320382742806096 27.0 2.0 34.0 2.0 37.0 12-13 20.987665136966807 27.0 2.0 34.0 2.0 37.0 14-15 22.523925906223496 29.0 2.0 35.0 2.0 37.5 16-17 23.660028089505005 32.0 2.0 37.0 2.0 38.5 18-19 23.683137407004594 32.0 2.0 38.0 2.0 39.5 20-21 23.917527765092014 32.0 2.0 38.0 2.0 40.0 22-23 23.863331796703104 32.0 2.0 38.0 2.0 40.0 24-25 23.92463939972484 32.5 2.0 38.5 2.0 40.0 26-27 23.786461331763725 32.5 2.0 38.0 2.0 40.0 28-29 23.72768450854479 32.0 2.0 38.0 2.0 40.0 30-31 23.9150532646701 32.0 2.0 38.0 2.0 40.0 32-33 23.869926521353545 32.5 2.0 38.0 2.0 40.0 34-35 23.63324770742178 32.0 2.0 38.0 2.0 40.0 36-37 23.10957810538511 31.0 2.0 38.0 2.0 40.0 38-39 23.153612598551646 31.0 2.0 38.0 2.0 40.0 40-41 23.17378929429958 31.0 2.0 38.0 2.0 40.0 42-43 23.114264708096417 31.0 2.0 38.0 2.0 40.0 44-45 22.775555678231065 30.0 2.0 38.0 2.0 40.0 46-47 22.484795963943917 30.0 2.0 38.0 2.0 40.0 48-49 22.399381070197236 30.0 2.0 38.0 2.0 40.0 50-51 19.563853796922487 20.5 2.0 36.0 2.0 39.0 52-53 21.868620983103995 29.0 2.0 37.0 2.0 39.0 54-55 22.847658885295452 31.0 2.0 38.0 2.0 40.0 56-57 22.92498962236894 31.0 2.0 38.0 2.0 40.0 58-59 22.678038208322608 31.0 2.0 38.0 2.0 40.0 60-61 22.440957193974594 31.0 2.0 38.0 2.0 40.0 62-63 22.04963447079817 30.0 2.0 38.0 2.0 40.0 64-65 21.78323046514012 29.0 2.0 37.0 2.0 40.0 66-67 21.52429225990033 29.0 2.0 37.0 2.0 39.5 68-69 21.14321095017518 28.5 2.0 36.0 2.0 39.0 70-71 20.8737194648512 28.0 2.0 36.0 2.0 39.0 72-73 20.5084583966327 28.0 2.0 35.5 2.0 39.0 74-75 20.11007780893258 26.0 2.0 35.0 2.0 38.0 76-77 19.776219559845057 26.0 2.0 35.0 2.0 37.5 78-79 19.46674677218035 26.0 2.0 34.0 2.0 37.0 80-81 19.107765694240218 26.0 2.0 34.0 2.0 36.0 82-83 18.698995287587636 25.0 2.0 34.0 2.0 36.0 84-85 18.380962657017683 24.5 2.0 34.0 2.0 35.5 86-87 17.93130904406066 22.0 2.0 34.0 2.0 35.0 88-89 17.580772024057467 20.0 2.0 33.0 2.0 35.0 90-91 17.164663431378948 18.5 2.0 33.0 2.0 35.0 92-93 16.657580115670257 13.5 2.0 32.5 2.0 35.0 94-95 16.148828851176525 4.5 2.0 32.0 2.0 35.0 96-97 15.547304074268709 2.0 2.0 32.0 2.0 34.0 98-99 14.870220823084153 2.0 2.0 32.0 2.0 34.0 100-101 13.671773632142195 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 9.066843881265292 1101 2 10.168286322072984 1101 3 9.662451002534098 1101 4 11.806365417798414 1101 5 12.615881941830335 1101 6 11.737849688959244 1101 7 11.955733600618018 1101 8 11.763313314385137 1101 9 12.697538379651593 1101 10-11 12.190419000896345 1101 12-13 11.632970317083903 1101 14-15 11.102878310937157 1101 16-17 11.069392262939047 1101 18-19 11.554958408984604 1101 20-21 11.694722069906629 1101 22-23 11.899095443305011 1101 24-25 12.150742491484035 1101 26-27 12.302884975998971 1101 28-29 12.052535995151995 1101 30-31 11.809830247715663 1101 32-33 11.909723139885202 1101 34-35 11.616823479347108 1101 36-37 12.323945200951766 1101 38-39 12.017252149277521 1101 40-41 11.619359131777397 1101 42-43 11.122095183304339 1101 44-45 11.123727940227852 1101 46-47 11.269327612747972 1101 48-49 11.244089358257852 1101 50-51 13.375434066139103 1101 52-53 11.97127472976307 1101 54-55 11.416178686996421 1101 56-57 11.46338601835166 1101 58-59 11.353994568333693 1101 60-61 11.285242435319311 1101 62-63 11.33590180294777 1101 64-65 11.11661101729229 1101 66-67 10.969045754281012 1101 68-69 11.011261425328524 1101 70-71 10.713331736935416 1101 72-73 10.576940348694372 1101 74-75 10.435372199469 1101 76-77 10.325076651789 1101 78-79 10.022046725578399 1101 80-81 9.856096523213655 1101 82-83 9.734734630381102 1101 84-85 9.49227658054075 1101 86-87 9.486014788725985 1101 88-89 9.262041849351537 1101 90-91 8.879276569734433 1101 92-93 8.773913395010045 1101 94-95 8.795605303311008 1101 96-97 8.795153603615327 1101 98-99 8.601504379236879 1101 100-101 8.02638172394847 1102 1 8.982498289251787 1102 2 9.81405597633416 1102 3 9.316373046136828 1102 4 11.480302142455248 1102 5 12.143470578694465 1102 6 11.254763731387492 1102 7 11.177862353462473 1102 8 11.136906502376036 1102 9 11.77952032195505 1102 10-11 10.83685884527717 1102 12-13 10.469548163373261 1102 14-15 10.017433107971584 1102 16-17 10.08525660999917 1102 18-19 10.979455949908044 1102 20-21 11.482759212973495 1102 22-23 11.806031507314412 1102 24-25 11.816015192063933 1102 26-27 12.066623506425458 1102 28-29 11.859582167067142 1102 30-31 11.771324779023015 1102 32-33 11.885258631333492 1102 34-35 11.821422319543831 1102 36-37 12.109658333154652 1102 38-39 11.94060970635012 1102 40-41 11.68101588873255 1102 42-43 11.300243902382377 1102 44-45 11.296810227536078 1102 46-47 11.44825956606222 1102 48-49 10.89879463609681 1102 50-51 12.854516500257876 1102 52-53 11.474161759051778 1102 54-55 11.288628954925652 1102 56-57 11.068243245100536 1102 58-59 11.16726257097628 1102 60-61 10.935660845361156 1102 62-63 11.02740587694029 1102 64-65 10.91001702184342 1102 66-67 10.839502335063507 1102 68-69 10.581384746182959 1102 70-71 10.44066639156362 1102 72-73 10.536557907235075 1102 74-75 10.396186263918828 1102 76-77 10.058647084146042 1102 78-79 9.809622883546542 1102 80-81 9.490273283105015 1102 82-83 9.517776544790557 1102 84-85 9.46835013262179 1102 86-87 9.301811602937246 1102 88-89 9.083294877318945 1102 90-91 9.085919746714538 1102 92-93 9.15922059606708 1102 94-95 9.075399768553615 1102 96-97 8.91724638787724 1102 98-99 8.0839818071162 1102 100-101 7.643866125328492 1103 1 9.0380500719343 1103 2 10.040847870276288 1103 3 9.444629376736838 1103 4 11.674566733239 1103 5 12.189914003660444 1103 6 11.240567774783557 1103 7 10.970583758897817 1103 8 10.559277873494295 1103 9 10.810177885562076 1103 10-11 9.972157283548555 1103 12-13 9.317799195896232 1103 14-15 9.403340945513271 1103 16-17 10.179591761536216 1103 18-19 11.26502422887588 1103 20-21 12.083045309133592 1103 22-23 12.489095481594656 1103 24-25 12.492135554741392 1103 26-27 12.535971963360517 1103 28-29 12.414052178746982 1103 30-31 12.166576738709463 1103 32-33 12.322887501036924 1103 34-35 12.250108998492404 1103 36-37 12.761240411204962 1103 38-39 12.379751658691507 1103 40-41 11.978598039029361 1103 42-43 11.62977273475844 1103 44-45 11.779811829000273 1103 46-47 11.834111051329955 1103 48-49 11.699136602887492 1103 50-51 13.748771458174286 1103 52-53 12.313406783073741 1103 54-55 11.9458395173647 1103 56-57 11.666337623118334 1103 58-59 11.52124395546014 1103 60-61 11.614841810815285 1103 62-63 11.701909122012477 1103 64-65 11.458913938121757 1103 66-67 11.489085833961752 1103 68-69 11.466512562291523 1103 70-71 11.276826489818866 1103 72-73 10.966785122493537 1103 74-75 11.099584739517567 1103 76-77 10.737242371265694 1103 78-79 10.490260820186354 1103 80-81 10.501551925834004 1103 82-83 10.000943831573057 1103 84-85 9.911950898434743 1103 86-87 9.86138727513077 1103 88-89 9.752961700519702 1103 90-91 9.742486351971674 1103 92-93 9.401577277062636 1103 94-95 9.081560950506438 1103 96-97 9.224110287502759 1103 98-99 9.152850547257561 1103 100-101 8.620559161969508 1104 1 8.626836974542304 1104 2 9.918688419274616 1104 3 9.362824194527644 1104 4 11.319811586379696 1104 5 11.682473626670077 1104 6 10.77487125052734 1104 7 10.817799473965508 1104 8 10.076706823973467 1104 9 10.26175182410303 1104 10-11 8.902005124106942 1104 12-13 8.632859656881315 1104 14-15 8.99722118966693 1104 16-17 9.84390302082788 1104 18-19 11.103145680390199 1104 20-21 11.800043394700037 1104 22-23 12.088581006918414 1104 24-25 12.179707322526898 1104 26-27 12.349991879478473 1104 28-29 12.103862772822293 1104 30-31 11.91743537013829 1104 32-33 12.036082304744106 1104 34-35 12.065866363991478 1104 36-37 12.16290598717342 1104 38-39 11.771282660966488 1104 40-41 11.56979957846303 1104 42-43 11.389602867510646 1104 44-45 11.400480998169911 1104 46-47 11.141581379487718 1104 48-49 11.129805988005998 1104 50-51 13.18821799064595 1104 52-53 11.782287614044936 1104 54-55 11.400046623584313 1104 56-57 11.341459939366981 1104 58-59 11.675252107059993 1104 60-61 11.408118665698595 1104 62-63 11.487975632382241 1104 64-65 11.302437162482295 1104 66-67 11.178483543213844 1104 68-69 11.00028555497649 1104 70-71 10.665744510938666 1104 72-73 10.768744529742094 1104 74-75 10.55393268194748 1104 76-77 10.349855474478918 1104 78-79 9.886251870514645 1104 80-81 9.790953437663777 1104 82-83 9.604367372063937 1104 84-85 9.472263301316442 1104 86-87 9.283112152532848 1104 88-89 8.957398604489125 1104 90-91 8.621975858265646 1104 92-93 8.307865566907314 1104 94-95 8.330276289541446 1104 96-97 7.8676188648974215 1104 98-99 7.684051166561325 1104 100-101 6.922936852762673 1105 1 8.44408615722681 1105 2 9.501763380437637 1105 3 8.84208223436163 1105 4 10.856704164459405 1105 5 10.880110483541326 1105 6 10.448531200908754 1105 7 9.981882573504102 1105 8 9.42024875631597 1105 9 8.619211074259539 1105 10-11 7.981699761126116 1105 12-13 7.193342394714328 1105 14-15 8.141381963160402 1105 16-17 9.62962770723378 1105 18-19 10.668685804204806 1105 20-21 11.365149935542629 1105 22-23 11.78815461168551 1105 24-25 11.84117130267946 1105 26-27 12.112683571843235 1105 28-29 11.909246157551824 1105 30-31 11.633911754937376 1105 32-33 11.455963359254994 1105 34-35 11.58807114499729 1105 36-37 11.764526010823399 1105 38-39 11.730874351012627 1105 40-41 11.386267152485157 1105 42-43 11.288171348115348 1105 44-45 11.32110401284681 1105 46-47 11.234706191099392 1105 48-49 11.13330548950151 1105 50-51 13.07378501051657 1105 52-53 11.423314301424657 1105 54-55 11.125149918351166 1105 56-57 10.900577470494529 1105 58-59 10.955261381562071 1105 60-61 10.892722992253809 1105 62-63 11.064027940573947 1105 64-65 10.879342029277257 1105 66-67 10.563552290460478 1105 68-69 10.69021019964713 1105 70-71 10.368320272027493 1105 72-73 10.243783598582084 1105 74-75 10.24787225856646 1105 76-77 10.06531375081271 1105 78-79 9.778043936178118 1105 80-81 9.248655679774654 1105 82-83 9.143681986360583 1105 84-85 9.101873772229286 1105 86-87 8.72119905461729 1105 88-89 8.514719200839611 1105 90-91 8.481489178769252 1105 92-93 8.492815600527386 1105 94-95 7.983553202987155 1105 96-97 7.927713232956036 1105 98-99 7.953877733372515 1105 100-101 7.3829145939576915 1106 1 8.218764373081587 1106 2 9.239575770461137 1106 3 8.780520823483748 1106 4 10.703572374297412 1106 5 11.047301556003777 1106 6 10.165210847167565 1106 7 9.312399902962479 1106 8 8.985174795345568 1106 9 7.84945980562739 1106 10-11 7.294762982713866 1106 12-13 7.101022529126894 1106 14-15 8.399135320636532 1106 16-17 9.842087434548816 1106 18-19 11.263551788969863 1106 20-21 11.793499862319567 1106 22-23 12.250464827721924 1106 24-25 11.960424540955255 1106 26-27 12.166447010262075 1106 28-29 12.199599558167119 1106 30-31 11.636863609354034 1106 32-33 12.156158620757822 1106 34-35 12.008641036127653 1106 36-37 12.362508005896455 1106 38-39 11.945600247001817 1106 40-41 11.703061458114814 1106 42-43 11.508390222637132 1106 44-45 11.135680511081517 1106 46-47 11.07004920425684 1106 48-49 11.377166218099369 1106 50-51 13.39330593868986 1106 52-53 12.013491966687415 1106 54-55 11.418355045100864 1106 56-57 11.171366122622533 1106 58-59 11.211030564301854 1106 60-61 10.972343140516323 1106 62-63 11.075486158018062 1106 64-65 11.079652631295719 1106 66-67 10.984515202127348 1106 68-69 10.673751698392685 1106 70-71 10.513200674357009 1106 72-73 10.38081426144948 1106 74-75 10.106582851502992 1106 76-77 10.028810066954705 1106 78-79 9.760495376231702 1106 80-81 9.41277501633964 1106 82-83 9.351297184971049 1106 84-85 9.19512067585865 1106 86-87 8.816474180345264 1106 88-89 8.692804048774036 1106 90-91 8.334621877124288 1106 92-93 8.243943689802762 1106 94-95 7.769690317430831 1106 96-97 7.330693468405082 1106 98-99 7.370553292762665 1106 100-101 6.921931247947496 1107 1 7.891700097016635 1107 2 8.730755026190035 1107 3 8.637302550424934 1107 4 10.167555976838361 1107 5 10.996497873096796 1107 6 10.24629285846482 1107 7 9.77813565334504 1107 8 8.486875542623714 1107 9 5.299890415546024 1107 10-11 6.9349194125167735 1107 12-13 6.788063518698696 1107 14-15 7.525323930362244 1107 16-17 9.225894309725156 1107 18-19 10.864596692230378 1107 20-21 11.539708787721725 1107 22-23 11.201381139000116 1107 24-25 11.378553180035897 1107 26-27 11.54924263030005 1107 28-29 11.630491387379575 1107 30-31 11.141710886278982 1107 32-33 11.520818219960233 1107 34-35 11.526041372246585 1107 36-37 11.9563278466951 1107 38-39 11.45442416135172 1107 40-41 11.24525203691233 1107 42-43 10.780100846975088 1107 44-45 10.263206441193958 1107 46-47 10.825520873489005 1107 48-49 11.023146082664546 1107 50-51 12.832863178312035 1107 52-53 11.20854985911939 1107 54-55 10.941730979764632 1107 56-57 10.776362529111783 1107 58-59 10.698460748977059 1107 60-61 10.400366727714093 1107 62-63 10.685484948859436 1107 64-65 10.626402915583856 1107 66-67 10.784797520288237 1107 68-69 10.498665981726177 1107 70-71 10.270627602908412 1107 72-73 9.888789500416813 1107 74-75 10.1586310167746 1107 76-77 9.910141337019606 1107 78-79 9.578179162682513 1107 80-81 9.401729103182593 1107 82-83 9.170046570768282 1107 84-85 8.955073025045547 1107 86-87 8.514038925808318 1107 88-89 8.767837776148166 1107 90-91 8.59507600449896 1107 92-93 8.410279988971247 1107 94-95 8.272254684748765 1107 96-97 8.070399656444193 1107 98-99 7.80018478921648 1107 100-101 7.254862887009571 1108 1 6.779899648842509 1108 2 7.98467339871835 1108 3 8.089507813815011 1108 4 9.21793010049949 1108 5 10.156691313620993 1108 6 9.191543230067612 1108 7 8.301252453212513 1108 8 5.705969038877079 1108 9 5.069705104817665 1108 10-11 4.6565367017025245 1108 12-13 3.824833513900046 1108 14-15 6.575961619663595 1108 16-17 8.543777879884935 1108 18-19 8.820618143007302 1108 20-21 9.216041853663103 1108 22-23 10.20416242048282 1108 24-25 9.878000615008784 1108 26-27 8.356460682588235 1108 28-29 10.074523414993873 1108 30-31 10.367174523223728 1108 32-33 11.151594043766039 1108 34-35 10.49127771702555 1108 36-37 10.38282206805783 1108 38-39 10.042378108714221 1108 40-41 10.326487423761872 1108 42-43 9.633997026871356 1108 44-45 9.152491541339895 1108 46-47 10.07824858654952 1108 48-49 10.498236316195857 1108 50-51 12.533882959014623 1108 52-53 11.237197711115694 1108 54-55 10.620577935232614 1108 56-57 10.45712286759218 1108 58-59 10.488247456625825 1108 60-61 10.417063419706466 1108 62-63 10.48557299913993 1108 64-65 10.454305806388003 1108 66-67 10.029095859487086 1108 68-69 10.405850137357302 1108 70-71 10.559371977098696 1108 72-73 10.113710475740223 1108 74-75 9.998259990178553 1108 76-77 9.618243178242803 1108 78-79 9.529175760412475 1108 80-81 9.530476754499876 1108 82-83 9.387659507857347 1108 84-85 9.108416009697969 1108 86-87 8.86874808528826 1108 88-89 8.616797108262478 1108 90-91 8.595897086869183 1108 92-93 8.391218633771196 1108 94-95 8.265713035075441 1108 96-97 8.049533630617914 1108 98-99 7.612928941930598 1108 100-101 7.13333256773277 1109 1 6.539780101377037 1109 2 8.020691552616636 1109 3 7.854973978848356 1109 4 8.855287707634016 1109 5 9.603554966880118 1109 6 8.867439725169415 1109 7 7.070342921972355 1109 8 7.03218520546287 1109 9 5.468785256443166 1109 10-11 3.874789113986921 1109 12-13 4.9587331511591834 1109 14-15 6.963184829046419 1109 16-17 6.612944817860594 1109 18-19 6.875307604360632 1109 20-21 9.460667274265969 1109 22-23 10.512707056947985 1109 24-25 9.992817323976002 1109 26-27 10.494169586816305 1109 28-29 10.92586242467318 1109 30-31 8.431364752374115 1109 32-33 7.680968158315508 1109 34-35 9.183625295337745 1109 36-37 7.864217515921698 1109 38-39 8.06155130084268 1109 40-41 9.096028102812053 1109 42-43 7.683850386948215 1109 44-45 8.359346314589153 1109 46-47 8.015383705524805 1109 48-49 8.851981400476113 1109 50-51 10.920818965281196 1109 52-53 10.779318321343585 1109 54-55 10.567323158789744 1109 56-57 9.734261455182708 1109 58-59 9.752228149479503 1109 60-61 9.802771660373367 1109 62-63 10.372740098570315 1109 64-65 10.377589751135002 1109 66-67 10.016544307082164 1109 68-69 9.487904843895596 1109 70-71 9.159347270977346 1109 72-73 8.65518548884733 1109 74-75 7.758251553725621 1109 76-77 7.894427674863341 1109 78-79 8.701494968823024 1109 80-81 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-21.27579602157978 2314 18-19 -21.26826334833757 2314 20-21 -21.46671447123703 2314 22-23 -21.42133691373822 2314 24-25 -21.461879544778167 2314 26-27 -21.344604563749982 2314 28-29 -21.28533011363462 2314 30-31 -21.4823594315033 2314 32-33 -21.438951894982296 2314 34-35 -21.222437329578977 2314 36-37 -20.691745175617275 2314 38-39 -20.73658327610602 2314 40-41 -20.754071830565696 2314 42-43 -20.69589644849482 2314 44-45 -20.336523105797255 2314 46-47 -20.061564995341286 2314 48-49 -19.973135188464596 2314 50-51 -17.257062447110545 2314 52-53 -19.485838240948222 2314 54-55 -20.44400262402171 2314 56-57 -20.527897105776656 2314 58-59 -20.246772516743018 2314 60-61 -20.031005821305513 2314 62-63 -19.65224324586673 2314 64-65 -19.394895258858337 2314 66-67 -19.131023980725963 2314 68-69 -18.75826437662406 2314 70-71 -18.50354413475217 2314 72-73 -18.140284198028084 2314 74-75 -17.74907689397591 2314 76-77 -17.43468624918729 2314 78-79 -17.12195606382188 2314 80-81 -16.7774460151406 2314 82-83 -16.358012928893654 2314 84-85 -16.052702498957157 2314 86-87 -15.626258572501353 2314 88-89 -15.269687578821408 2314 90-91 -14.894782007671427 2314 92-93 -14.399726772353972 2314 94-95 -13.894073915656913 2314 96-97 -13.3139816822982 2314 98-99 -12.624088368322397 2314 100-101 -11.464204050110107 2315 1 -18.800659605485055 2315 2 -18.07857560261321 2315 3 -18.875205901231592 2315 4 -20.455838208421948 2315 5 -19.58565222832308 2315 6 -20.329095917735316 2315 7 -20.103541155309458 2315 8 -19.944497006395892 2315 9 -18.949602485062496 2315 10-11 -18.94033413717897 2315 12-13 -18.658522012065333 2315 14-15 -20.146075663958243 2315 16-17 -21.27579602157978 2315 18-19 -21.26826334833757 2315 20-21 -21.46671447123703 2315 22-23 -21.42133691373822 2315 24-25 -21.461879544778167 2315 26-27 -21.344604563749982 2315 28-29 -21.28533011363462 2315 30-31 -21.4823594315033 2315 32-33 -21.438951894982296 2315 34-35 -21.222437329578977 2315 36-37 -20.691745175617275 2315 38-39 -20.73658327610602 2315 40-41 -20.754071830565696 2315 42-43 -20.69589644849482 2315 44-45 -20.336523105797255 2315 46-47 -20.061564995341286 2315 48-49 -19.973135188464596 2315 50-51 -17.257062447110545 2315 52-53 -19.485838240948222 2315 54-55 -20.44400262402171 2315 56-57 -20.527897105776656 2315 58-59 -20.246772516743018 2315 60-61 -20.031005821305513 2315 62-63 -19.65224324586673 2315 64-65 -19.394895258858337 2315 66-67 -19.131023980725963 2315 68-69 -18.75826437662406 2315 70-71 -18.50354413475217 2315 72-73 -18.140284198028084 2315 74-75 -17.74907689397591 2315 76-77 -17.43468624918729 2315 78-79 -17.12195606382188 2315 80-81 -16.7774460151406 2315 82-83 -16.358012928893654 2315 84-85 -16.052702498957157 2315 86-87 -15.626258572501353 2315 88-89 -15.269687578821408 2315 90-91 -14.894782007671427 2315 92-93 -14.399726772353972 2315 94-95 -13.894073915656913 2315 96-97 -13.3139816822982 2315 98-99 -12.624088368322397 2315 100-101 -11.464204050110107 2316 1 -18.800659605485055 2316 2 -18.07857560261321 2316 3 -18.875205901231592 2316 4 -20.455838208421948 2316 5 -19.58565222832308 2316 6 -20.329095917735316 2316 7 -20.103541155309458 2316 8 -19.944497006395892 2316 9 -18.949602485062496 2316 10-11 -18.94033413717897 2316 12-13 -18.658522012065333 2316 14-15 -20.146075663958243 2316 16-17 -21.27579602157978 2316 18-19 -21.26826334833757 2316 20-21 -21.46671447123703 2316 22-23 -21.42133691373822 2316 24-25 -21.461879544778167 2316 26-27 -21.344604563749982 2316 28-29 -21.28533011363462 2316 30-31 -21.4823594315033 2316 32-33 -21.438951894982296 2316 34-35 -21.222437329578977 2316 36-37 -20.691745175617275 2316 38-39 -20.73658327610602 2316 40-41 -20.754071830565696 2316 42-43 -20.69589644849482 2316 44-45 -20.336523105797255 2316 46-47 -20.061564995341286 2316 48-49 -19.973135188464596 2316 50-51 -17.257062447110545 2316 52-53 -19.485838240948222 2316 54-55 -20.44400262402171 2316 56-57 -20.527897105776656 2316 58-59 -20.246772516743018 2316 60-61 -20.031005821305513 2316 62-63 -19.65224324586673 2316 64-65 -19.394895258858337 2316 66-67 -19.131023980725963 2316 68-69 -18.75826437662406 2316 70-71 -18.50354413475217 2316 72-73 -18.140284198028084 2316 74-75 -17.74907689397591 2316 76-77 -17.43468624918729 2316 78-79 -17.12195606382188 2316 80-81 -16.7774460151406 2316 82-83 -16.358012928893654 2316 84-85 -16.052702498957157 2316 86-87 -15.626258572501353 2316 88-89 -15.269687578821408 2316 90-91 -14.894782007671427 2316 92-93 -14.399726772353972 2316 94-95 -13.894073915656913 2316 96-97 -13.3139816822982 2316 98-99 -12.624088368322397 2316 100-101 -11.464204050110107 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 374815.0 3 5837.0 4 21851.0 5 11051.0 6 8109.0 7 9425.0 8 7609.0 9 7103.0 10 8095.0 11 9226.0 12 10137.0 13 11025.0 14 11833.0 15 12828.0 16 14006.0 17 13642.0 18 13052.0 19 13964.0 20 14951.0 21 16478.0 22 18216.0 23 19920.0 24 22085.0 25 24864.0 26 27940.0 27 31431.0 28 35333.0 29 40297.0 30 46713.0 31 53854.0 32 63415.0 33 74767.0 34 86244.0 35 97622.0 36 92051.0 37 54835.0 38 10116.0 39 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.31019322122596 21.802065252241594 15.113489369700053 22.774252156832393 2 13.833866429330103 20.878530052337574 43.15110866754351 22.13649485078881 3 17.612812836652335 27.142992700865737 31.363112858421356 23.881081604060576 4 11.066586651041995 17.48358294288547 42.36391430488791 29.085916101184623 5 11.388631946978505 38.53403796705675 37.77251164724751 12.304818438717232 6 27.448984760173662 40.730005552354115 18.99306481346097 12.827944874011255 7 23.746078476644257 35.11347797774262 24.340538538597027 16.7999050070161 8 22.336627881983752 39.88789101121986 21.102246836803413 16.673234269992978 9 23.562414851867665 19.843510441448586 21.97824274588747 34.61583196079627 10-11 22.189045611972755 30.559262656551688 29.23577459186196 18.015917139613595 12-13 22.808067686377395 27.873678133105788 29.514512411008802 19.80374176950802 14-15 20.444798833431573 28.693014109570154 27.546703565164115 23.315483491834154 16-17 19.94121308720617 31.163530527230588 28.375060131437785 20.52019625412546 18-19 19.78354390077223 29.813955290988286 30.51406974456689 19.88843106367259 20-21 20.632356206973128 28.95524195990552 30.612470474229305 19.79993135889205 22-23 20.292329169388935 28.694534684984408 30.32408313436925 20.689053011257403 24-25 20.141136030000993 28.81258523568065 30.60638150384501 20.43989723047335 26-27 20.22297618981923 28.890331494345826 30.38919744926396 20.49749486657098 28-29 20.2045896755395 28.82678247347484 30.54627493018682 20.422352920798843 30-31 20.512194146987106 28.713183022995636 30.442901762339115 20.331721067678146 32-33 20.386109708823412 28.902001428305073 30.201155398520967 20.510733464350547 34-35 20.46030371356182 28.82575409047663 30.283897907167116 20.430044288794434 36-37 20.57515229485568 28.668818261060668 30.32315894224525 20.432870501838405 38-39 20.464567949728945 28.83031342233603 30.39356631815578 20.31155230977925 40-41 20.547248910371366 28.863342161205658 30.29264990558531 20.296759022837666 42-43 20.55562551488195 28.82978447293872 30.05660937798896 20.557980634190372 44-45 20.544225402698917 29.13392264305647 29.86439260220176 20.457459352042854 46-47 20.658292286160442 28.96588779923611 29.638511614860146 20.737308299743297 48-49 20.62715960371533 29.002217789327666 29.71777306821444 20.65284953874256 50-51 20.836577454584805 29.206009465318715 29.18261171050113 20.774801369595345 52-53 20.424722137951957 29.386221351940208 29.361491859492357 20.82756465061548 54-55 20.451073741890557 29.27557579440948 29.46531893686003 20.808031526839933 56-57 20.49389166788506 29.299189630336254 29.486382386144385 20.720536315634313 58-59 20.466531796657573 29.216663017037988 29.642695810495447 20.674109375808992 60-61 20.428059293285685 29.404120611367567 29.488738959949835 20.679081135396913 62-63 20.46065219327114 29.14153280919533 29.338473259208968 21.059341738324562 64-65 20.196140789444488 29.156296918104562 29.590901863041662 21.056660429409284 66-67 20.378931265318883 29.08103445388004 29.34772494216743 21.192309338633645 68-69 20.3113667478033 29.19439096862062 29.32644025382493 21.167802029751147 70-71 20.363647791359355 28.816196713462496 29.561090989589133 21.259064505589016 72-73 20.42758303157578 28.983211223212624 29.408939651103832 21.180266094107758 74-75 20.161322325841667 29.281295215843294 29.18574138934283 21.371641068972206 76-77 20.25487413874809 29.236529054449882 29.288322298023555 21.220274508778473 78-79 20.21523450646284 29.217265568375307 29.16129570683041 21.406204218331446 80-81 20.206578603912455 29.23083568931225 29.105469424969577 21.457116281805718 82-83 20.3398571106013 28.956474391316995 29.39723122753125 21.306437270550454 84-85 20.50111827614669 28.93469106335944 29.157362603164437 21.406828057329427 86-87 20.155522186391398 29.054057134208772 29.305237591183975 21.485183088215855 88-89 20.42473661747198 28.954961026124852 29.193296942740975 21.427005413662194 90-91 20.339990977320262 28.864327149607877 29.349820229941624 21.445861643130232 92-93 20.31761359366139 28.83246372586754 29.110221892244592 21.73970078822648 94-95 19.99873033082086 28.93855206386526 29.170604367031643 21.89211323828224 96-97 20.03236951704663 28.883797964113718 29.12603636418549 21.95779615465416 98-99 19.951069694602236 28.702839880006557 29.041401616490102 22.3046888089011 100-101 20.219124761224045 28.74709462999404 28.939375662766437 22.09440494601548 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 84895.0 1 76951.5 2 51188.5 3 26080.5 4 16007.0 5 12409.5 6 11810.0 7 12254.0 8 12626.0 9 12203.5 10 10750.0 11 8761.0 12 6671.0 13 5019.5 14 3975.5 15 3471.5 16 3487.5 17 3879.5 18 4572.5 19 5329.0 20 5991.5 21 6767.5 22 7619.5 23 8516.5 24 9442.5 25 10614.0 26 12299.0 27 14466.0 28 16995.0 29 20216.5 30 24325.0 31 28400.5 32 31924.5 33 35431.0 34 38823.0 35 41101.5 36 42795.0 37 44142.0 38 43705.0 39 42195.0 40 41107.0 41 39962.0 42 38972.0 43 38463.5 44 38219.5 45 37972.0 46 37145.5 47 36371.0 48 35509.5 49 33787.0 50 31143.0 51 28483.5 52 25984.5 53 23347.0 54 20472.5 55 17320.5 56 14023.5 57 10899.5 58 8418.5 59 6547.5 60 5013.5 61 3561.0 62 2424.5 63 1581.5 64 991.5 65 595.5 66 355.0 67 193.5 68 100.5 69 67.0 70 45.0 71 31.5 72 19.0 73 11.5 74 8.5 75 5.5 76 2.5 77 2.0 78 1.5 79 0.0 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.18475839656101 2 29.193584580740122 3 22.935102346025708 4 29.276247161834405 5 32.41369718251798 6 24.97972866886001 7 23.923827112609665 8 19.94763508306048 9 11.854660111970876 10-11 15.470269789730196 12-13 15.560496032841348 14-15 19.663764753621777 16-17 22.948939187440452 18-19 26.930687461599177 20-21 28.97613825944006 22-23 28.563829062672287 24-25 27.085366142181073 26-27 28.54644339405532 28-29 25.495993409935423 30-31 27.36228220417299 32-33 26.364237285340764 34-35 24.41951582526005 36-37 27.415550458945802 38-39 27.54076311972739 40-41 24.539925022959835 42-43 25.418205985401777 44-45 24.147044758955772 46-47 27.413722275235568 48-49 22.836523812630528 50-51 28.45284755743902 52-53 22.013984529981137 54-55 23.146347181406725 56-57 25.220654604477833 58-59 24.534117851174376 60-61 24.085782681293093 62-63 23.916406837550465 64-65 22.45117136390391 66-67 22.954316198352913 68-69 23.935548996398836 70-71 22.434932790948267 72-73 22.29936042247533 74-75 19.60597980968249 76-77 21.860524638539403 78-79 20.966614497711902 80-81 21.996634708103585 82-83 22.842259290937157 84-85 19.829555923422763 86-87 21.365086853064934 88-89 20.210535069940576 90-91 21.33540575282813 92-93 21.300813649291275 94-95 20.382563572400016 96-97 19.291030357169742 98-99 18.695150007850437 100-101 20.357578395026767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1394827.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.03214685390805 #Duplication Level Percentage of deduplicated Percentage of total 1 87.82694014375033 66.77670810739016 2 8.246128792845543 12.539417507077433 3 1.956647375365848 4.463043017553897 4 0.766949143125363 2.332511595183462 5 0.37412526964565773 1.4222773721728297 6 0.21843817432035365 0.9964994009054815 7 0.13689624991158944 0.7285961043919087 8 0.08966418827599144 0.5453888584429313 9 0.06606692667484641 0.4520889244015468 >10 0.30094341559135473 3.832333269590963 >50 0.012290936019558467 0.6233534078489433 >100 0.004524346630508305 0.5675868844801977 >500 1.925189215147815E-4 0.10465903242629024 >1k 9.625946075739075E-5 0.08336128526821299 >5k 0.0 0.0 >10k+ 9.625946075739075E-5 4.532175232865749 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 61925 4.439618676724784 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.169347883285884E-5 0.0 2 0.0 0.0 0.0 7.169347883285884E-5 0.0 3 0.0 0.0 0.0 7.169347883285884E-5 0.0 4 0.0 0.0 0.0 7.169347883285884E-5 0.0 5 0.0 0.0 0.0 7.169347883285884E-5 0.0 6 0.0 0.0 0.0 7.169347883285884E-5 0.0 7 0.0 0.0 0.0 7.169347883285884E-5 0.0 8 0.0 0.0 0.0 7.169347883285884E-5 0.0 9 0.0 0.0 0.0 7.169347883285884E-5 0.0 10-11 0.0 0.0 0.0 1.4338695766571768E-4 0.0 12-13 0.0 0.0 0.0 1.4338695766571768E-4 0.0 14-15 0.0 0.0 0.0 1.4338695766571768E-4 0.0 16-17 0.0 0.0 0.0 2.1508043649857652E-4 0.0 18-19 0.0 0.0 0.0 2.1508043649857652E-4 0.0 20-21 0.0 0.0 0.0 2.1508043649857652E-4 0.0 22-23 0.0 0.0 0.0 2.1508043649857652E-4 0.0 24-25 0.0 0.0 0.0 2.1508043649857652E-4 0.0 26-27 0.0 0.0 0.0 2.5092717591500594E-4 0.0 28-29 0.0 0.0 0.0 6.452413094957296E-4 0.0 30-31 0.0 0.0 0.0 0.002294191322651483 0.0 32-33 0.0 0.0 0.0 0.006990114186203737 0.0 34-35 0.0 3.584673941642942E-5 0.0 0.013299140323495315 0.0 36-37 0.0 7.169347883285884E-5 0.0 0.02469840345791987 0.0 38-39 0.0 7.169347883285884E-5 0.0 0.041761451420140275 0.0 40-41 0.0 7.169347883285884E-5 0.0 0.05868111242469497 0.0 42-43 0.0 7.169347883285884E-5 0.0 0.07710633648473969 0.0 44-45 0.0 7.169347883285884E-5 0.0 0.10216320733682385 0.0 46-47 0.0 7.169347883285884E-5 0.0 0.1330272499743696 0.0 48-49 0.0 7.169347883285884E-5 0.0 0.16568362958273677 0.0 50-51 0.0 7.169347883285884E-5 0.0 0.20253407770282622 0.0 52-53 0.0 7.169347883285884E-5 0.0 0.24777266284636013 0.0 54-55 0.0 7.169347883285884E-5 0.0 0.2976713241140299 0.0 56-57 0.0 7.169347883285884E-5 0.0 0.3572486050241356 0.0 58-59 0.0 7.169347883285884E-5 0.0 0.4303042599548188 0.0 60-61 0.0 7.169347883285884E-5 0.0 0.5082709181855527 0.0 62-63 0.0 7.169347883285884E-5 0.0 0.5864526568527852 0.0 64-65 0.0 1.4338695766571768E-4 0.0 0.6717320499244709 0.0 66-67 0.0 1.4338695766571768E-4 0.0 0.7670485300327567 0.0 68-69 0.0 1.4338695766571768E-4 0.0 0.8796431385397616 0.0 70-71 0.0 1.4338695766571768E-4 0.0 0.9995863286271344 0.0 72-73 0.0 1.4338695766571768E-4 0.0 1.1259102383306319 0.0 74-75 0.0 1.4338695766571768E-4 0.0 1.259582729614497 0.0 76-77 0.0 1.4338695766571768E-4 0.0 1.4039375492444583 0.0 78-79 0.0 1.4338695766571768E-4 0.0 1.5690834777359486 0.0 80-81 0.0 1.4338695766571768E-4 0.0 1.7554506759619652 0.0 82-83 0.0 1.4338695766571768E-4 0.0 1.9569093514822984 0.0 84-85 0.0 1.4338695766571768E-4 3.584673941642942E-5 2.151879767168258 0.0 86-87 0.0 1.4338695766571768E-4 7.169347883285884E-5 2.3591814612134696 0.0 88-89 0.0 1.4338695766571768E-4 7.169347883285884E-5 2.5921494206808444 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1045 0.0 63.235268 1 TTACGCG 20 6.889279E-4 45.191086 80-81 GTATCAA 1985 0.0 42.580364 1 ATCAACG 2215 0.0 34.109406 3 TCAACGC 2245 0.0 33.6536 4 CAACGCA 2285 0.0 33.270584 5 TATCAAC 2490 0.0 30.851961 2 AACGCAG 2630 0.0 30.24629 6 GCCGATA 70 0.0058098487 29.265293 2 ACGCAGA 2960 0.0 26.87992 7 CGCAGAG 2975 0.0 26.691896 8 GCAGAGT 3325 0.0 22.893955 9 GTGTAGC 530 0.0 22.22992 1 GTGGTAT 540 0.0 21.818256 1 GAGTACT 2270 0.0 21.6839 12-13 GTACATG 2190 0.0 21.519375 1 ACATGGG 2085 0.0 21.25853 3 TACATGG 2130 0.0 20.9185 2 GAGTACA 1445 0.0 20.561089 1 TAGCCCG 125 0.004307181 20.13862 5 >>END_MODULE