FastQCFastQC Report
Sat 18 Jun 2016
SRR1930991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1394827
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29070.20841294296712068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.19271207110272456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15120.10840053999528257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.055.5510831
GTATCAA35500.040.5629121
ATCAACG42150.033.3680653
CAACGCA43100.032.5787625
TCAACGC43500.032.330184
TATCAAC45700.031.0924032
AACGCAG45950.030.8667036
GGCGTCG1303.0739466E-729.1911478
ACGCAGA51300.027.4627277
CGCAGAG51550.027.329548
GTGGTAT9650.024.623941
GCAGAGT57650.024.1909319
TACGGGC1652.4250512E-623.0362474
ACGGGCG1652.458546E-622.9999125
CGGGCGT1703.187084E-622.3226436
TGGTATC10200.021.8976252
AGAGTAC54400.021.36346810-11
GCGTCGG1805.2311407E-621.0824999
GAGTACT41400.020.45231212-13
CAGAGTA58350.020.40503910-11
Produced by FastQC (version 0.11.5)