##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930991_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1394827 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.380397712404477 31.0 31.0 34.0 30.0 34.0 2 31.551057586353004 31.0 31.0 34.0 30.0 34.0 3 31.615917959718303 31.0 31.0 34.0 30.0 34.0 4 35.38294283090304 37.0 35.0 37.0 33.0 37.0 5 35.26179017182776 37.0 35.0 37.0 33.0 37.0 6 35.28048711417258 37.0 35.0 37.0 32.0 37.0 7 35.166885929222765 37.0 35.0 37.0 32.0 37.0 8 35.17338781081811 37.0 35.0 37.0 32.0 37.0 9 36.682672474794366 39.0 35.0 39.0 32.0 39.0 10-11 36.62196387078828 39.0 35.0 39.0 32.0 39.0 12-13 36.5118061236268 38.0 35.0 39.0 32.0 39.0 14-15 37.59130702230456 39.0 36.0 41.0 32.0 41.0 16-17 37.5499273386592 39.0 36.0 41.0 32.0 41.0 18-19 37.57194404754138 39.0 36.0 41.0 32.0 41.0 20-21 37.55708306478151 39.0 36.0 41.0 32.0 41.0 22-23 37.46779600624306 39.0 36.0 41.0 32.0 41.0 24-25 37.35096825627838 39.0 36.0 41.0 31.5 41.0 26-27 37.11758017302505 39.0 36.0 40.0 31.0 41.0 28-29 36.93348601654542 39.0 36.0 40.0 31.0 41.0 30-31 36.719198509922734 38.5 35.5 40.0 30.0 41.0 32-33 36.56540488533703 38.0 35.0 40.0 30.0 41.0 34-35 36.644905784014796 38.5 35.0 40.0 30.0 41.0 36-37 36.7175918590621 39.0 35.0 40.0 30.0 41.0 38-39 36.70822761532433 39.0 35.0 40.0 30.0 41.0 40-41 36.60700681876678 39.0 35.0 40.0 30.0 41.0 42-43 36.47411184326084 39.0 35.0 40.0 30.0 41.0 44-45 36.3301004353945 39.0 35.0 40.0 30.0 41.0 46-47 36.10696559501644 39.0 35.0 40.0 29.5 41.0 48-49 35.90482260524065 38.5 35.0 40.0 29.0 41.0 50-51 35.66357082276153 38.0 35.0 40.0 27.5 41.0 52-53 35.474024018749276 38.0 34.0 40.0 27.0 41.0 54-55 35.34171083582409 38.0 34.0 40.0 27.0 41.0 56-57 35.18561190742651 38.0 34.0 40.0 27.0 41.0 58-59 35.001834277655945 38.0 34.0 40.0 26.5 41.0 60-61 34.721494493582355 38.0 33.5 40.0 26.0 41.0 62-63 34.48489884408605 37.5 33.0 40.0 26.0 41.0 64-65 34.19124593946059 37.0 33.0 40.0 26.0 41.0 66-67 33.89165681478779 37.0 32.5 39.5 25.0 41.0 68-69 33.566565244291944 36.0 32.0 39.0 24.5 41.0 70-71 33.218837891724206 36.0 32.0 39.0 23.5 40.0 72-73 32.812395372329334 35.0 31.5 38.5 22.5 40.0 74-75 32.304888348160745 35.0 31.0 38.0 22.0 40.0 76-77 30.890963180380076 34.0 29.5 36.0 20.5 39.0 78-79 31.642285745830847 34.5 31.0 37.0 22.0 39.0 80-81 31.60961932913544 35.0 31.0 36.0 22.0 39.0 82-83 31.32909099121253 34.0 31.0 36.0 21.5 37.5 84-85 30.976484897410216 34.0 31.0 35.5 20.5 37.0 86-87 30.62110426597707 34.0 30.0 35.0 20.0 37.0 88-89 30.26146289109689 34.0 30.0 35.0 18.0 36.0 90-91 29.925353108306624 34.0 30.0 35.0 17.0 36.0 92-93 29.603479499608195 34.0 29.5 35.0 9.5 36.0 94-95 29.2270389087679 34.0 29.0 35.0 4.5 35.0 96-97 28.815393593614118 34.0 29.0 35.0 2.0 35.0 98-99 28.340639376782928 34.0 29.0 35.0 2.0 35.0 100-101 27.236207787775832 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3800750096187713 1101 2 0.4189884687526728 1101 3 0.3116036042331771 1101 4 0.22875702381148244 1101 5 0.2953132220387573 1101 6 0.41054322898608575 1101 7 0.4133354581489428 1101 8 0.35688670437574643 1101 9 0.5018918285813143 1101 10-11 0.4271373163469292 1101 12-13 0.46892207280123444 1101 14-15 0.4971321772522117 1101 16-17 0.46524964614179254 1101 18-19 0.5588781159447862 1101 20-21 0.4032335385668375 1101 22-23 0.5689630629029878 1101 24-25 0.46909268549887173 1101 26-27 0.35859187183876173 1101 28-29 0.43471540219330507 1101 30-31 0.5184060427776771 1101 32-33 0.5683285648469507 1101 34-35 0.5191804696191724 1101 36-37 0.5066211795350029 1101 38-39 0.6153261697379548 1101 40-41 0.5470197415740969 1101 42-43 0.5667011742481662 1101 44-45 0.4759546955900049 1101 46-47 0.5975950968791963 1101 48-49 0.570907440543678 1101 50-51 0.5817778195620846 1101 52-53 0.5062850866799806 1101 54-55 0.4819865327337922 1101 56-57 0.6162351348096635 1101 58-59 0.4831055906274173 1101 60-61 0.46623073487576505 1101 62-63 0.2285255592734856 1101 64-65 0.3753196712531164 1101 66-67 0.3410367449213041 1101 68-69 0.17646798184959067 1101 70-71 0.22042204021665412 1101 72-73 0.2426483897847973 1101 74-75 0.16051504537204409 1101 76-77 0.20061962607392658 1101 78-79 0.3293697167366858 1101 80-81 0.18206301276979175 1101 82-83 0.2266181617838967 1101 84-85 0.2765316165374081 1101 86-87 0.2520584604281808 1101 88-89 0.44686163819554636 1101 90-91 0.4401231900799054 1101 92-93 0.30889162762457545 1101 94-95 0.46112367366376006 1101 96-97 0.6210235781257119 1101 98-99 0.8701610714954278 1101 100-101 0.9205454253056651 1102 1 0.2549938967948684 1102 2 0.43767598251616135 1102 3 0.3602067019355921 1102 4 0.28236489285648503 1102 5 0.2313757633292184 1102 6 0.2765653973777802 1102 7 0.27795118155788856 1102 8 0.3146023323578362 1102 9 0.5205059370743044 1102 10-11 0.4596118080154312 1102 12-13 0.685517536355519 1102 14-15 0.5853007157532772 1102 16-17 0.6070370644784262 1102 18-19 0.5076750035613102 1102 20-21 0.39964842515569643 1102 22-23 0.49544841855152555 1102 24-25 0.5916904980218121 1102 26-27 0.5912572172105612 1102 28-29 0.6011148736753569 1102 30-31 0.6621570521001487 1102 32-33 0.6068817469654277 1102 34-35 0.6568748100728214 1102 36-37 0.7928217227340042 1102 38-39 0.6824567577141707 1102 40-41 0.7993782529152114 1102 42-43 0.6976378254997257 1102 44-45 0.7233767024900999 1102 46-47 0.6976853853619005 1102 48-49 0.6797043281602129 1102 50-51 0.36385812492800795 1102 52-53 0.5919196752434388 1102 54-55 0.567325298057149 1102 56-57 0.37754468483534964 1102 58-59 0.37338086039178364 1102 60-61 0.22328938568689694 1102 62-63 0.033179453423088034 1102 64-65 0.25512294512818556 1102 66-67 0.08995181502334049 1102 68-69 0.17837872104065866 1102 70-71 0.4103097301527896 1102 72-73 0.20174330279954944 1102 74-75 0.37686606937557343 1102 76-77 0.3975072426047923 1102 78-79 0.3748919952362826 1102 80-81 0.38157633582638795 1102 82-83 0.33559929662937904 1102 84-85 0.3512655224318486 1102 86-87 0.35780682716091405 1102 88-89 0.5417306097877059 1102 90-91 0.4672772677426842 1102 92-93 0.4299281100440133 1102 94-95 0.46172412877643865 1102 96-97 0.7341858771787848 1102 98-99 0.7796743945520248 1102 100-101 0.7728224567965256 1103 1 0.3949864313226783 1103 2 0.4518960212884515 1103 3 0.45360264890665647 1103 4 0.3380380496675244 1103 5 0.36429440172342 1103 6 0.3806586435336641 1103 7 0.43737417280402013 1103 8 0.37739080663205726 1103 9 0.4045016504483385 1103 10-11 0.4345458830795508 1103 12-13 0.4499777947571886 1103 14-15 0.5694479059662783 1103 16-17 0.5931698294725862 1103 18-19 0.5481393077672649 1103 20-21 0.6078016840751275 1103 22-23 0.5019640900225895 1103 24-25 0.44239068706683327 1103 26-27 0.6009767803600212 1103 28-29 0.5507267895322627 1103 30-31 0.6246411277663171 1103 32-33 0.7097850931827168 1103 34-35 0.7229529085448121 1103 36-37 0.6341493726756013 1103 38-39 0.5890388959698996 1103 40-41 0.7204316069592096 1103 42-43 0.6729355413060532 1103 44-45 0.7195483601861596 1103 46-47 0.6795016624994972 1103 48-49 0.6832525948461381 1103 50-51 0.6318668908372871 1103 52-53 0.7161017846005677 1103 54-55 0.7310158903580728 1103 56-57 0.6961618867282127 1103 58-59 0.8073432199626396 1103 60-61 0.5425216461596278 1103 62-63 0.5571664972165138 1103 64-65 0.5695122815440286 1103 66-67 0.6108688639944333 1103 68-69 0.44729658880547873 1103 70-71 0.5847498799438782 1103 72-73 0.6616344513985695 1103 74-75 0.7482215342216278 1103 76-77 0.6649686208272847 1103 78-79 0.7707643115796401 1103 80-81 0.5187861758003756 1103 82-83 0.6025537452079313 1103 84-85 0.6601277603877556 1103 86-87 0.7152322025417099 1103 88-89 0.6666183266777352 1103 90-91 0.4832502475497833 1103 92-93 0.573898055497903 1103 94-95 0.5129668890745762 1103 96-97 0.8090130274153928 1103 98-99 0.8025717202272276 1103 100-101 0.9558630713734644 1104 1 0.39783883403537246 1104 2 0.34658903121177076 1104 3 0.37405507114863923 1104 4 0.205656706521971 1104 5 0.2739653426125841 1104 6 0.3401461687316072 1104 7 0.3833789781355108 1104 8 0.5020566727231142 1104 9 0.5006984155344796 1104 10-11 0.5441967313360436 1104 12-13 0.5329394769799976 1104 14-15 0.5744292827247364 1104 16-17 0.6019483851878604 1104 18-19 0.6274678852182589 1104 20-21 0.5115741697497356 1104 22-23 0.36564449698290247 1104 24-25 0.4400873108473107 1104 26-27 0.6191293942331413 1104 28-29 0.43970560494950917 1104 30-31 0.47275584730978437 1104 32-33 0.47262466102210965 1104 34-35 0.6687849547916613 1104 36-37 0.5543562704576175 1104 38-39 0.5310040493358414 1104 40-41 0.3849430883965326 1104 42-43 0.49846341889497126 1104 44-45 0.49270206788717985 1104 46-47 0.5076040260127783 1104 48-49 0.40807906116233994 1104 50-51 0.4633704488294157 1104 52-53 0.4135795019648256 1104 54-55 0.6819776608382426 1104 56-57 0.9473834061255886 1104 58-59 0.7290788948075146 1104 60-61 0.6488086215330071 1104 62-63 0.4186235698701566 1104 64-65 0.4193118716167703 1104 66-67 0.47557761001697685 1104 68-69 0.3882601619891375 1104 70-71 0.36770743097118697 1104 72-73 0.43782341415865744 1104 74-75 0.3730165001721275 1104 76-77 0.4644655069386765 1104 78-79 0.7766763809611916 1104 80-81 0.6880987636469662 1104 82-83 0.5679810375754535 1104 84-85 0.8133242259622229 1104 86-87 0.8783041031827032 1104 88-89 0.9271951260715774 1104 90-91 0.8252540839281544 1104 92-93 0.8558702225071109 1104 94-95 1.0498941434163207 1104 96-97 1.0877224888858912 1104 98-99 1.265275709741502 1104 100-101 1.4714381478709981 1105 1 0.18360525570060915 1105 2 0.28386093642635757 1105 3 0.30168752263140064 1105 4 0.28871636475657425 1105 5 0.21784022957959337 1105 6 0.29887880802303357 1105 7 0.34206060766553037 1105 8 0.3564697126730323 1105 9 0.4334527107882522 1105 10-11 0.28425052344807966 1105 12-13 0.3310245241640217 1105 14-15 0.43718891081720557 1105 16-17 0.2881551138391174 1105 18-19 0.3665272188452846 1105 20-21 0.39528327499214555 1105 22-23 0.3621150852181927 1105 24-25 0.47600212454575086 1105 26-27 0.37117395821561416 1105 28-29 0.31016631584009247 1105 30-31 0.17304613922473777 1105 32-33 0.48983744128597095 1105 34-35 0.535768654854266 1105 36-37 0.41315475871378027 1105 38-39 0.5982938079669182 1105 40-41 0.5286735249937138 1105 42-43 0.5294903381372507 1105 44-45 0.5690145258620873 1105 46-47 0.5810395334439704 1105 48-49 0.5692910067210164 1105 50-51 0.5038462307858751 1105 52-53 0.49146769784231026 1105 54-55 0.6990172397758485 1105 56-57 0.6563850059771923 1105 58-59 0.6448884429074013 1105 60-61 0.6215112114377135 1105 62-63 0.5604348801254346 1105 64-65 0.6512514018632416 1105 66-67 0.8767909467509654 1105 68-69 0.6948758961819053 1105 70-71 0.7228937325316167 1105 72-73 0.74572784041478 1105 74-75 0.8206424595034392 1105 76-77 0.7320954079333681 1105 78-79 0.8133517038297953 1105 80-81 0.8308716079048857 1105 82-83 0.6945109826240277 1105 84-85 0.6629098875820141 1105 86-87 0.5177949330187808 1105 88-89 0.6643592151995428 1105 90-91 0.6865279367881776 1105 92-93 0.647305451703243 1105 94-95 0.6596486009738847 1105 96-97 0.6816256630842261 1105 98-99 0.7375572372519947 1105 100-101 0.9045917104391066 1106 1 0.22782335825830557 1106 2 0.2545058670063547 1106 3 0.29208247307811774 1106 4 0.12069111699015878 1106 5 0.22829988878015683 1106 6 0.22241985267207554 1106 7 0.3086568136891188 1106 8 0.29787491434864677 1106 9 0.3907076973356425 1106 10-11 0.4828571282819425 1106 12-13 0.4513762219173856 1106 14-15 0.31515183383649514 1106 16-17 0.38835173763150266 1106 18-19 0.40630698203394644 1106 20-21 0.4263353031007213 1106 22-23 0.41920818635684043 1106 24-25 0.3195867624605384 1106 26-27 0.37564635482350894 1106 28-29 0.32423422597439355 1106 30-31 0.4118445998375435 1106 32-33 0.4995737247330254 1106 34-35 0.45222715610870523 1106 36-37 0.2637983270042241 1106 38-39 0.3347200683685685 1106 40-41 0.37000539008163713 1106 42-43 0.4169654479718403 1106 44-45 0.39900260582848546 1106 46-47 0.35936550662957956 1106 48-49 0.4701335828105613 1106 50-51 0.2871509181971774 1106 52-53 0.2987069045356989 1106 54-55 0.40523155062762584 1106 56-57 0.5023629595912311 1106 58-59 0.6440869625527483 1106 60-61 0.5854012292610022 1106 62-63 0.3797664500506315 1106 64-65 0.4403296994553898 1106 66-67 0.25820318692260713 1106 68-69 0.2626743227374675 1106 70-71 0.4464540762826843 1106 72-73 0.4654358563535581 1106 74-75 0.9130853852472995 1106 76-77 0.7249311271654868 1106 78-79 0.47949358628691385 1106 80-81 0.3989951675674952 1106 82-83 0.49322611652573656 1106 84-85 0.43957000769054133 1106 86-87 0.6784338468199067 1106 88-89 0.5191539068112441 1106 90-91 0.6685611539484846 1106 92-93 0.6057479006457704 1106 94-95 0.8677633057543837 1106 96-97 0.7940815305788611 1106 98-99 0.9169101497135372 1106 100-101 1.05649902933974 1107 1 0.12395799059936152 1107 2 0.12679878953370505 1107 3 0.09706873531519022 1107 4 0.052992272665029816 1107 5 0.06918367052113439 1107 6 0.03366158612551118 1107 7 0.07838817668261555 1107 8 0.13733034915438935 1107 9 0.11201655947208167 1107 10-11 0.1537223378898176 1107 12-13 0.14691890997211488 1107 14-15 0.17684763579170237 1107 16-17 0.07868077822526942 1107 18-19 0.2037883091376287 1107 20-21 0.14261415591260374 1107 22-23 -0.020089106803446555 1107 24-25 0.0992418307145968 1107 26-27 0.185339278347179 1107 28-29 0.27339272855596874 1107 30-31 0.1690308288340674 1107 32-33 0.17299967871163346 1107 34-35 0.16923308913762014 1107 36-37 0.19108441801175502 1107 38-39 0.21349693422034477 1107 40-41 0.15584030052917797 1107 42-43 0.034298442559617115 1107 44-45 0.26237673807601425 1107 46-47 0.15868819664975575 1107 48-49 0.1749116161570754 1107 50-51 0.0747986608519895 1107 52-53 0.2911759294692686 1107 54-55 0.3335492758515315 1107 56-57 0.26983179205573293 1107 58-59 0.24462601180988486 1107 60-61 0.10345663952127637 1107 62-63 -0.15528714918891495 1107 64-65 -0.06558521472377521 1107 66-67 -0.08426404918078134 1107 68-69 -0.10724670154216653 1107 70-71 -0.1385703808752865 1107 72-73 -0.12554042375822405 1107 74-75 0.008046454415250537 1107 76-77 0.16997441459155382 1107 78-79 0.1496224318755459 1107 80-81 0.051209444968414175 1107 82-83 0.1742242565952168 1107 84-85 0.2731650989593746 1107 86-87 0.16351409736482125 1107 88-89 0.19098508471742193 1107 90-91 0.15988464826294901 1107 92-93 0.006400121716193752 1107 94-95 0.20468131891201935 1107 96-97 0.3943289325860668 1107 98-99 0.34224868016463716 1107 100-101 0.5456476231607112 1108 1 0.03182525615198273 1108 2 0.0826354517618455 1108 3 0.09828505360705009 1108 4 -0.024061626170777117 1108 5 -0.06283593101983342 1108 6 -0.014190320145054613 1108 7 0.0316026871527626 1108 8 0.09067540451892597 1108 9 0.11872071503118065 1108 10-11 0.13746589729989722 1108 12-13 0.04492440906325612 1108 14-15 -0.060349061152372485 1108 16-17 0.06521259651758271 1108 18-19 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-1.1539641691997105 2316 70-71 -1.159348385597056 2316 72-73 -2.030495635118662 2316 74-75 -3.123589674676129 2316 76-77 -1.954670116518475 2316 78-79 -1.8203554631142005 2316 80-81 -1.4695215653122098 2316 82-83 -1.1535954539307731 2316 84-85 -1.0733751409120025 2316 86-87 -0.9009801925168652 2316 88-89 -0.9388141491557 2316 90-91 -0.9239504975872386 2316 92-93 -0.8207070355959338 2316 94-95 -1.2629183636405266 2316 96-97 -1.144733140608949 2316 98-99 -1.2506531619587165 2316 100-101 -1.6973694434580189 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 11.0 5 20.0 6 57.0 7 152.0 8 401.0 9 784.0 10 1426.0 11 2272.0 12 3252.0 13 4305.0 14 5353.0 15 6663.0 16 7817.0 17 9412.0 18 7935.0 19 6144.0 20 5968.0 21 6435.0 22 7224.0 23 8406.0 24 10309.0 25 13516.0 26 17630.0 27 22738.0 28 29005.0 29 36884.0 30 45431.0 31 54861.0 32 67146.0 33 82227.0 34 102019.0 35 127211.0 36 167792.0 37 234408.0 38 243234.0 39 56363.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.357134868034265 22.882138524587813 15.465285684377768 22.29544092300015 2 14.651418738460986 22.313430963093083 41.707361666278295 21.327788632167632 3 17.882302670350832 27.34417456864722 31.835434234921472 22.93808852608048 4 11.969942458898188 18.702940830051222 40.902228963599434 28.424887747451155 5 11.529229405919441 39.115453608203076 37.229440333124465 12.125876652753023 6 28.01506429857308 41.13681970661469 18.284926883091092 12.563189111721146 7 24.42453436877835 34.08630604368857 24.299787715609174 17.189371871923903 8 23.071419200026384 38.8707941735893 21.08499943720377 16.972787189180547 9 24.212966912742584 17.12133476051152 22.32233818244126 36.34336014430463 10-11 22.995719182378892 28.2625372178772 30.114523163087608 18.6272204366563 12-13 23.50219776359362 25.64515169264719 30.388786566362707 20.46386397739648 14-15 20.749347410108925 27.64106229661456 27.890949917086495 23.718640376190024 16-17 19.940071421043612 30.740012919164883 28.72456584221556 20.595349817575944 18-19 19.756249341316163 29.655792438775563 30.92229358909743 19.66566463081085 20-21 20.553122358543387 28.818233372310686 30.982874578711193 19.64576969043473 22-23 20.30082680771209 28.503734346126482 30.8390135662423 20.35642527991913 24-25 20.085559703057502 28.80839795657562 30.934306365250457 20.171735975116423 26-27 20.143584956523732 28.88845154518549 30.85918679881616 20.108776699474614 28-29 20.172336907972753 28.940527348930978 30.794557887507118 20.092577855589155 30-31 20.483427766355796 28.681444941753853 30.73317189200041 20.101955399889942 32-33 20.40729541915458 28.943883845831976 30.498178009690267 20.150642725323173 34-35 20.378435141256183 28.90274459948165 30.684316026362897 20.03450423289927 36-37 20.461236629504047 28.770292558406467 30.78392805356694 19.98454275852254 38-39 20.337856003441292 28.884734644130983 30.84064309142724 19.936766261000482 40-41 20.38324422274838 28.911835188135953 30.70083398904146 20.004086600074203 42-43 20.408512267629206 28.867397174623527 30.446722680881027 20.277367876866244 44-45 20.318726099396397 29.237543185547377 30.287583391081363 20.156147323974867 46-47 20.491395400029113 29.000022227623774 30.076376983367286 20.43220538897983 48-49 20.537601911295003 29.033278086754695 30.058620169195784 20.370499832754515 50-51 20.534697703820502 29.22077875432352 29.731232423074577 20.513291118781403 52-53 20.551086209099378 29.355854245711654 29.59889290364443 20.49416664154454 54-55 20.574873409030364 29.25498216091852 29.687056868862044 20.48308756118907 56-57 20.54018747138762 29.362140559445827 29.61452105602596 20.483150913140598 58-59 20.46199733463076 29.30826963612368 29.844964714512567 20.384768314732995 60-61 20.489663664890198 29.458671707631684 29.71873554278725 20.332929084690868 62-63 20.500697789065047 29.291905790571292 29.716256401190627 20.49114001917303 64-65 20.222241706181574 29.198150429261972 29.780848577535217 20.79875928702124 66-67 20.420414161099707 29.186990613579855 29.666715421379074 20.725879803941368 68-69 20.35195020806532 29.368257641638934 29.583294403438288 20.69649774685746 70-71 20.367384732797547 28.99704176348696 29.771939475623565 20.86363402809193 72-73 20.469243084851033 29.10206202261263 29.707358601060434 20.721336291475904 74-75 20.273512631264854 29.430038215552973 29.503125910001117 20.793323243181057 76-77 20.263132367364975 29.38607237936473 29.56879919810016 20.781996055170136 78-79 20.24738652100516 29.385143688019383 29.480107589906506 20.887362201068942 80-81 20.295864860563253 29.292793509728092 29.439012464637166 20.972329165071486 82-83 20.34030026662805 29.164692108770478 29.68167378463422 20.81333383996725 84-85 20.466946796986292 29.132860204168686 29.364071673404656 21.03612132544036 86-87 20.135041836729574 29.205198924311045 29.51434120503833 21.14541803392105 88-89 20.284300897962108 29.152976179807506 29.435233810693095 21.12748911153729 90-91 20.322678941443865 29.067274669649322 29.566582779462692 21.04346360944412 92-93 20.275156071403476 29.11859275438599 29.42499896024105 21.181252213969483 94-95 19.949583573364613 29.011061573355292 29.49683588886734 21.542518964412757 96-97 19.951585376389104 29.055119041687266 29.49026276744814 21.503032814475493 98-99 19.8735040259473 29.132573430253355 29.394147087775043 21.599775456024297 100-101 19.99280197472518 28.92261907749133 29.342097622142386 21.7424813256411 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 114.0 1 84.0 2 71.0 3 88.0 4 81.5 5 97.0 6 156.5 7 311.0 8 549.0 9 707.5 10 696.0 11 657.0 12 696.0 13 833.0 14 966.5 15 1072.5 16 1246.0 17 1463.0 18 1672.0 19 1865.0 20 2158.5 21 2526.0 22 3009.0 23 3872.0 24 5137.5 25 6697.5 26 9049.5 27 12368.0 28 16267.5 29 21522.5 30 27680.0 31 33577.0 32 39834.5 33 45721.5 34 51150.5 35 54547.5 36 57651.5 37 61016.0 38 60136.5 39 57861.0 40 56447.0 41 54521.5 42 53178.5 43 52821.0 44 52883.0 45 53248.0 46 52743.0 47 51578.0 48 50456.5 49 48412.0 50 45112.0 51 41170.5 52 37260.0 53 33475.0 54 29412.0 55 24675.0 56 19569.5 57 14889.0 58 11376.5 59 8764.0 60 6368.0 61 4395.5 62 2881.0 63 1759.5 64 998.0 65 574.0 66 330.0 67 170.0 68 88.5 69 43.5 70 23.5 71 18.0 72 10.5 73 7.0 74 6.5 75 2.0 76 0.5 77 1.5 78 2.0 79 1.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0050902369971329775 2 0.007312734840951602 3 0.0020791108861529066 4 0.17428684704267985 5 0.002365884801484342 6 4.30160872997153E-4 7 0.0 8 4.30160872997153E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.226206547478648E-4 24-25 8.244750065778767E-4 26-27 0.00308281958981293 28-29 7.169347883285884E-5 30-31 0.007133501143869455 32-33 0.02405316214842414 34-35 0.015449944688481081 36-37 8.244750065778767E-4 38-39 1.4338695766571768E-4 40-41 0.0017564902314050415 42-43 0.01297651966874745 44-45 0.008495677241693773 46-47 0.011901117486254569 48-49 0.012223738141002433 50-51 0.19586658417137034 52-53 0.1170754509340585 54-55 0.01982324689728547 56-57 0.007707048974532326 58-59 0.01960816646078689 60-61 0.12241661510710648 62-63 0.23579985188127273 64-65 0.24153533018790144 66-67 0.25153657048508526 68-69 0.24641048674853583 70-71 0.23576400514185628 72-73 0.16392713935133174 74-75 0.2890681066540869 76-77 0.2245439757045139 78-79 0.00620148591904229 80-81 7.169347883285884E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.4338695766571768E-4 90-91 0.006954267446787308 92-93 0.019572319721370465 94-95 0.00311866632922936 96-97 3.584673941642942E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1394827.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.92977942746509 #Duplication Level Percentage of deduplicated Percentage of total 1 76.41047877345996 46.55673617613918 2 14.486437033608054 17.653108262952006 3 4.236061754982656 7.743069251166418 4 1.7501954694147497 4.265560956255577 5 0.8792469631178204 2.6786161762518668 6 0.5164528172085936 1.888041374232753 7 0.34154295305326426 1.4567095756178319 8 0.24270787395038537 1.1830509780084786 9 0.18243874415569142 1.0004357196387015 >10 0.8686676300760268 9.488781562997408 >50 0.05923370862569231 2.471444012630111 >100 0.02523897717912834 2.666058382758548 >500 9.434917585516366E-4 0.4376897027655635 >1k 3.538094094568637E-4 0.5106978685855951 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2907 0.20841294296712068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2688 0.19271207110272456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1512 0.10840053999528257 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.169347883285884E-5 0.0 5 0.0 0.0 0.0 7.169347883285884E-5 0.0 6 0.0 0.0 0.0 7.169347883285884E-5 0.0 7 0.0 0.0 0.0 7.169347883285884E-5 0.0 8 0.0 0.0 0.0 7.169347883285884E-5 0.0 9 0.0 0.0 0.0 7.169347883285884E-5 0.0 10-11 0.0 0.0 0.0 7.169347883285884E-5 0.0 12-13 0.0 0.0 0.0 7.169347883285884E-5 0.0 14-15 0.0 0.0 0.0 7.169347883285884E-5 7.169347883285884E-5 16-17 0.0 0.0 0.0 1.4338695766571768E-4 1.4338695766571768E-4 18-19 0.0 0.0 0.0 1.792336970821471E-4 1.4338695766571768E-4 20-21 0.0 0.0 0.0 2.8677391533143536E-4 1.4338695766571768E-4 22-23 0.0 0.0 0.0 2.8677391533143536E-4 1.4338695766571768E-4 24-25 0.0 0.0 0.0 3.584673941642942E-4 1.4338695766571768E-4 26-27 0.0 0.0 0.0 5.735478306628707E-4 1.4338695766571768E-4 28-29 0.0 0.0 0.0 0.0012187891401586003 1.4338695766571768E-4 30-31 0.0 0.0 0.0 0.0037280608993086597 1.4338695766571768E-4 32-33 0.0 0.0 0.0 0.011972810965087427 1.4338695766571768E-4 34-35 0.0 0.0 0.0 0.0235154610571777 1.4338695766571768E-4 36-37 0.0 0.0 0.0 0.04222745903255386 1.4338695766571768E-4 38-39 0.0 0.0 0.0 0.07205194622702313 1.4338695766571768E-4 40-41 0.0 0.0 0.0 0.1059988084543818 1.4338695766571768E-4 42-43 3.584673941642942E-5 0.0 0.0 0.14284925657447123 1.4338695766571768E-4 44-45 7.169347883285884E-5 0.0 0.0 0.18962925151291166 1.4338695766571768E-4 46-47 7.169347883285884E-5 0.0 0.0 0.24906314546535163 1.4338695766571768E-4 48-49 7.169347883285884E-5 0.0 0.0 0.3117949394441031 1.4338695766571768E-4 50-51 7.169347883285884E-5 0.0 0.0 0.37868495519516043 1.4338695766571768E-4 52-53 7.169347883285884E-5 0.0 0.0 0.4509519818586821 1.4338695766571768E-4 54-55 7.169347883285884E-5 0.0 0.0 0.5279866248645889 1.4338695766571768E-4 56-57 7.169347883285884E-5 0.0 0.0 0.6240558865006198 1.4338695766571768E-4 58-59 7.169347883285884E-5 0.0 0.0 0.7401634754704347 1.4338695766571768E-4 60-61 7.169347883285884E-5 0.0 0.0 0.8661289177797676 1.4338695766571768E-4 62-63 1.4338695766571768E-4 0.0 0.0 0.9928829883562621 1.4338695766571768E-4 64-65 1.4338695766571768E-4 0.0 0.0 1.1287421307445296 1.4338695766571768E-4 66-67 2.1508043649857652E-4 0.0 0.0 1.281628474355601 1.4338695766571768E-4 68-69 2.1508043649857652E-4 0.0 0.0 1.4590698344669266 1.4338695766571768E-4 70-71 2.1508043649857652E-4 0.0 0.0 1.653681782758722 1.4338695766571768E-4 72-73 2.1508043649857652E-4 0.0 0.0 1.8604816224521032 1.4338695766571768E-4 74-75 2.1508043649857652E-4 0.0 0.0 2.073232020888612 1.4338695766571768E-4 76-77 2.1508043649857652E-4 0.0 0.0 2.308530018418055 1.4338695766571768E-4 78-79 2.1508043649857652E-4 0.0 0.0 2.5789219738361817 1.4338695766571768E-4 80-81 2.1508043649857652E-4 0.0 0.0 2.881109987116682 1.4338695766571768E-4 82-83 2.1508043649857652E-4 0.0 0.0 3.2044834233922916 1.4338695766571768E-4 84-85 2.1508043649857652E-4 0.0 0.0 3.5188593280743774 1.4338695766571768E-4 86-87 2.1508043649857652E-4 0.0 0.0 3.8530226329143327 1.4338695766571768E-4 88-89 2.1508043649857652E-4 0.0 0.0 4.229054929392677 1.4338695766571768E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1865 0.0 55.551083 1 GTATCAA 3550 0.0 40.562912 1 ATCAACG 4215 0.0 33.368065 3 CAACGCA 4310 0.0 32.578762 5 TCAACGC 4350 0.0 32.33018 4 TATCAAC 4570 0.0 31.092403 2 AACGCAG 4595 0.0 30.866703 6 GGCGTCG 130 3.0739466E-7 29.191147 8 ACGCAGA 5130 0.0 27.462727 7 CGCAGAG 5155 0.0 27.32954 8 GTGGTAT 965 0.0 24.62394 1 GCAGAGT 5765 0.0 24.190931 9 TACGGGC 165 2.4250512E-6 23.036247 4 ACGGGCG 165 2.458546E-6 22.999912 5 CGGGCGT 170 3.187084E-6 22.322643 6 TGGTATC 1020 0.0 21.897625 2 AGAGTAC 5440 0.0 21.363468 10-11 GCGTCGG 180 5.2311407E-6 21.082499 9 GAGTACT 4140 0.0 20.452312 12-13 CAGAGTA 5835 0.0 20.405039 10-11 >>END_MODULE