FastQCFastQC Report
Sat 18 Jun 2016
SRR1930990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121022
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN942974.445828473254874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.052.740391
GTATCAA32500.041.9396741
ATCAACG36300.035.2416533
TCAACGC37000.034.1641354
CAACGCA37150.034.0201765
TATCAAC39000.033.8937342
AACGCAG39550.031.1406636
ACGCAGA46550.026.2844947
GTACATG31400.025.7842331
GTGGTAT10450.025.4984551
CGCAGAG47100.024.7986058
TGGTATC10100.024.3491692
TACATGG32600.024.0457382
GCAGAGT51700.021.2538159
ACATGGG33450.020.77843
GAGTACA24500.019.6602291
GAGTACT35200.019.5136812-13
GTGTAGC8050.019.096431
CAGAGTA50050.018.59150510-11
AGAGTAC47500.018.2868210-11
Produced by FastQC (version 0.11.5)