##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930990_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2121022 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.688842925721655 28.0 2.0 31.0 2.0 31.0 2 20.088672347575837 28.0 2.0 31.0 2.0 33.0 3 20.80285164416022 28.0 2.0 31.0 2.0 33.0 4 22.439323118760672 32.0 2.0 35.0 2.0 37.0 5 21.637903802978 30.0 2.0 35.0 2.0 37.0 6 22.18522202975735 30.0 2.0 35.0 2.0 37.0 7 21.900904846814413 30.0 2.0 35.0 2.0 37.0 8 21.637980652723073 28.0 2.0 35.0 2.0 36.0 9 20.4703496710548 25.0 2.0 33.0 2.0 37.0 10-11 20.467194352533824 26.0 2.0 33.5 2.0 37.0 12-13 20.056341235498735 25.0 2.0 33.0 2.0 37.0 14-15 21.55534831793352 27.0 2.0 35.0 2.0 37.5 16-17 22.84500302212801 30.5 2.0 36.5 2.0 38.5 18-19 23.01634353627638 31.5 2.0 37.5 2.0 39.5 20-21 23.297044537963302 32.0 2.0 38.0 2.0 40.0 22-23 23.283427281753795 32.0 2.0 38.0 2.0 40.0 24-25 23.338281969729685 32.0 2.0 38.0 2.0 40.0 26-27 23.187368164969527 31.5 2.0 38.0 2.0 40.0 28-29 23.110951701585368 31.0 2.0 38.0 2.0 40.0 30-31 23.235401377260587 31.0 2.0 38.0 2.0 40.0 32-33 23.181320844385397 31.0 2.0 38.0 2.0 40.0 34-35 22.91243042269246 30.0 2.0 38.0 2.0 40.0 36-37 22.46010201685791 30.0 2.0 38.0 2.0 40.0 38-39 22.450837615074242 30.0 2.0 38.0 2.0 40.0 40-41 22.419056709454217 30.0 2.0 38.0 2.0 40.0 42-43 22.351030305201924 30.0 2.0 38.0 2.0 40.0 44-45 22.005538367824567 30.0 2.0 38.0 2.0 40.0 46-47 21.698523164776226 29.0 2.0 37.5 2.0 40.0 48-49 21.557412652956923 28.5 2.0 37.5 2.0 40.0 50-51 18.846910828836286 16.0 2.0 35.5 2.0 38.5 52-53 21.062623584290968 27.5 2.0 36.5 2.0 39.0 54-55 21.984271261684228 30.0 2.0 38.0 2.0 40.0 56-57 22.024158872468085 30.0 2.0 38.0 2.0 40.0 58-59 21.78293412326699 29.5 2.0 38.0 2.0 40.0 60-61 21.5381707497612 29.0 2.0 38.0 2.0 40.0 62-63 21.131682745393494 28.0 2.0 37.0 2.0 40.0 64-65 20.83433127992072 28.0 2.0 36.5 2.0 39.5 66-67 20.532887211919537 27.5 2.0 36.0 2.0 39.0 68-69 20.1292996960899 26.0 2.0 36.0 2.0 39.0 70-71 19.821159799379732 26.0 2.0 35.0 2.0 39.0 72-73 19.446194098882522 25.5 2.0 35.0 2.0 38.0 74-75 19.030343391063365 24.5 2.0 34.0 2.0 37.5 76-77 18.66333823977309 23.5 2.0 34.0 2.0 37.0 78-79 18.336604476521224 22.0 2.0 34.0 2.0 36.0 80-81 17.947109223760997 21.0 2.0 34.0 2.0 36.0 82-83 17.521666913403067 19.5 2.0 33.5 2.0 35.5 84-85 17.181776285205906 18.0 2.0 33.0 2.0 35.0 86-87 16.729844150602872 14.0 2.0 32.5 2.0 35.0 88-89 16.35608588689792 7.5 2.0 32.0 2.0 35.0 90-91 15.919756372163985 4.5 2.0 32.0 2.0 35.0 92-93 15.407172108540127 2.0 2.0 32.0 2.0 34.0 94-95 14.870399034050568 2.0 2.0 31.0 2.0 34.0 96-97 14.244171206144962 2.0 2.0 31.0 2.0 34.0 98-99 13.546537706822466 2.0 2.0 31.0 2.0 34.0 100-101 12.388162168992118 2.0 2.0 29.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 9.394193998144562 1101 2 10.41033506257746 1101 3 9.845778787319912 1101 4 11.94694008273596 1101 5 12.602198938922324 1101 6 12.066514919256466 1101 7 12.20212465959533 1101 8 12.205850271895518 1101 9 13.320392893968354 1101 10-11 12.871125037185688 1101 12-13 12.426943142461578 1101 14-15 11.801301470755021 1101 16-17 11.340527730010262 1101 18-19 12.052733575382302 1101 20-21 12.054104211489364 1101 22-23 12.265871171556356 1101 24-25 12.324481227502137 1101 26-27 12.316214453102816 1101 28-29 12.409378707507486 1101 30-31 12.267107227418716 1101 32-33 12.350619052106303 1101 34-35 12.251228618145074 1101 36-37 12.69648478854587 1101 38-39 12.5392262855367 1101 40-41 12.202140756924326 1101 42-43 12.050988752843299 1101 44-45 11.993835072689766 1101 46-47 12.010880013710182 1101 48-49 12.226779323538427 1101 50-51 13.922463526686897 1101 52-53 12.829478876879197 1101 54-55 12.427414123899492 1101 56-57 12.354185708280923 1101 58-59 12.417616843829062 1101 60-61 12.265237262744922 1101 62-63 12.298221391988172 1101 64-65 11.992831520151931 1101 66-67 11.978622586313964 1101 68-69 11.965806962269156 1101 70-71 11.724677737218691 1101 72-73 11.909472521251278 1101 74-75 11.611464054302864 1101 76-77 11.29544328391696 1101 78-79 11.067750727231317 1101 80-81 11.06389968567219 1101 82-83 10.77117500067379 1101 84-85 10.302501464777368 1101 86-87 10.001915214330847 1101 88-89 9.976236221369422 1101 90-91 9.873533327461335 1101 92-93 9.84946024138489 1101 94-95 9.630209464018389 1101 96-97 9.50382331230696 1101 98-99 9.26518325573757 1101 100-101 8.496354764577355 1102 1 9.277175453941553 1102 2 10.020680907551139 1102 3 9.360218740271193 1102 4 11.481783400750796 1102 5 11.975611275117831 1102 6 11.366991860510108 1102 7 11.576578205569913 1102 8 11.454935838133185 1102 9 11.99232817431787 1102 10-11 11.187721302002018 1102 12-13 10.820396626529945 1102 14-15 10.05161627042257 1102 16-17 10.209416302782326 1102 18-19 11.173036441639862 1102 20-21 11.532795632737415 1102 22-23 11.745699625494836 1102 24-25 12.01636150352481 1102 26-27 12.009974636345206 1102 28-29 12.090623957522965 1102 30-31 12.126521186347237 1102 32-33 11.994596763236117 1102 34-35 11.886641539156695 1102 36-37 12.219498422400928 1102 38-39 12.011265423433443 1102 40-41 11.803486821862624 1102 42-43 11.386945855790689 1102 44-45 11.347156538470788 1102 46-47 11.474263148614359 1102 48-49 11.169630341632168 1102 50-51 13.13703703899504 1102 52-53 12.030368153011192 1102 54-55 11.621013285136197 1102 56-57 11.438172349710939 1102 58-59 11.544492964232692 1102 60-61 11.51556194106313 1102 62-63 11.468552708759617 1102 64-65 11.381356874696706 1102 66-67 11.096271388428786 1102 68-69 11.144310557439418 1102 70-71 10.70004927326984 1102 72-73 10.550667416491482 1102 74-75 10.250260742452305 1102 76-77 10.102331798734276 1102 78-79 9.916612311529796 1102 80-81 9.472065114873217 1102 82-83 9.62295177478066 1102 84-85 9.473065917704279 1102 86-87 9.475065700426313 1102 88-89 9.58135000366767 1102 90-91 9.264074585316507 1102 92-93 9.088705025128053 1102 94-95 8.765016823744844 1102 96-97 8.802582026923279 1102 98-99 8.393542820181276 1102 100-101 7.8048218877694 1103 1 9.22206685055172 1103 2 10.13544339415255 1103 3 9.438640833536496 1103 4 11.862180198490993 1103 5 12.264542482153342 1103 6 11.404945650690376 1103 7 11.242214785252624 1103 8 10.970784453155613 1103 9 11.06611151534955 1103 10-11 10.398156478044175 1103 12-13 9.807633651351384 1103 14-15 9.85728429044373 1103 16-17 10.477288280446412 1103 18-19 11.462483007242898 1103 20-21 11.902286510565194 1103 22-23 12.204284730445451 1103 24-25 12.279302548899256 1103 26-27 12.352942664789389 1103 28-29 12.19912085182331 1103 30-31 12.058953655680408 1103 32-33 12.256286013467644 1103 34-35 12.361707300674336 1103 36-37 12.42999871405586 1103 38-39 12.133290086286863 1103 40-41 11.874646843901107 1103 42-43 11.573232591761371 1103 44-45 11.656968747144052 1103 46-47 11.794991569667875 1103 48-49 11.807565899440863 1103 50-51 13.673957171335168 1103 52-53 12.273082923731767 1103 54-55 11.908297802344887 1103 56-57 11.628427042078528 1103 58-59 11.64912755746911 1103 60-61 11.493733612284739 1103 62-63 11.711084210537798 1103 64-65 11.32122796258745 1103 66-67 11.492384784537773 1103 68-69 11.273851242409453 1103 70-71 11.026759088482233 1103 72-73 10.861088876244885 1103 74-75 10.880218587444801 1103 76-77 10.662170421240791 1103 78-79 10.355489336073973 1103 80-81 9.86858075742883 1103 82-83 9.78275546591168 1103 84-85 9.480322842697188 1103 86-87 9.325195921149337 1103 88-89 9.081461960511565 1103 90-91 9.164341097218067 1103 92-93 9.171803469642093 1103 94-95 8.946117626284266 1103 96-97 8.837168079621136 1103 98-99 8.445284526290154 1103 100-101 7.658564659673814 1104 1 8.793180877400502 1104 2 9.831404056142716 1104 3 9.318699632737022 1104 4 11.20473566145829 1104 5 11.608592373865829 1104 6 10.954151779987495 1104 7 10.930312327543835 1104 8 10.240275084197759 1104 9 10.010213564357034 1104 10-11 9.305944621631578 1104 12-13 8.63650923379149 1104 14-15 9.187478270758426 1104 16-17 9.9818056930371 1104 18-19 11.256825876260852 1104 20-21 11.889229563356121 1104 22-23 12.121285767479165 1104 24-25 12.218192128238453 1104 26-27 12.471196801209231 1104 28-29 12.231215313574282 1104 30-31 12.036398681687746 1104 32-33 11.996036799392513 1104 34-35 11.943037841419166 1104 36-37 12.151748204197926 1104 38-39 11.735529182661722 1104 40-41 11.519710033686426 1104 42-43 11.117712271808973 1104 44-45 11.478754095426428 1104 46-47 11.189720524912435 1104 48-49 11.005391999684736 1104 50-51 13.331815197548288 1104 52-53 12.018542794194229 1104 54-55 11.563195087109452 1104 56-57 11.344742903397169 1104 58-59 11.371028833489188 1104 60-61 11.01801110131846 1104 62-63 11.060839621347803 1104 64-65 11.117272259615095 1104 66-67 11.025238277548493 1104 68-69 10.800983630255988 1104 70-71 10.454567993117916 1104 72-73 10.351716760431241 1104 74-75 10.231969422267127 1104 76-77 9.898245442175021 1104 78-79 9.594572875353911 1104 80-81 9.602994690761214 1104 82-83 9.276082184504741 1104 84-85 8.953177517396622 1104 86-87 8.778165219654095 1104 88-89 8.422989252427385 1104 90-91 8.20029244831612 1104 92-93 7.9270255150637645 1104 94-95 7.673813048055457 1104 96-97 7.4656136699867055 1104 98-99 7.1557353405347985 1104 100-101 6.4465953754308245 1105 1 8.709559030582767 1105 2 9.470003879156845 1105 3 8.994550010074875 1105 4 10.981646959719253 1105 5 10.884658514877831 1105 6 10.737179033823022 1105 7 10.319326277609957 1105 8 10.084708480835026 1105 9 9.224193459557533 1105 10-11 8.326680803310563 1105 12-13 8.00164997733101 1105 14-15 8.656381455509802 1105 16-17 9.886378988056645 1105 18-19 10.889369899010493 1105 20-21 11.5639071607232 1105 22-23 12.263062387446752 1105 24-25 12.029017101143705 1105 26-27 12.283673807960554 1105 28-29 12.264153577323007 1105 30-31 11.833407819329064 1105 32-33 11.794794915387854 1105 34-35 11.739179947153314 1105 36-37 12.01375242004114 1105 38-39 12.038911800262866 1105 40-41 11.510597747088628 1105 42-43 11.087419350249508 1105 44-45 11.138234771154504 1105 46-47 11.18143947532656 1105 48-49 10.906330621382413 1105 50-51 13.049792686536385 1105 52-53 11.59667459895297 1105 54-55 11.284729196661718 1105 56-57 11.255619580040005 1105 58-59 11.394985064286711 1105 60-61 11.109644541705048 1105 62-63 11.049597041331296 1105 64-65 10.772036957893956 1105 66-67 10.65277499217369 1105 68-69 10.507619606114318 1105 70-71 10.370864116331902 1105 72-73 10.047806665276479 1105 74-75 10.100248184956918 1105 76-77 9.70957701227833 1105 78-79 9.34586994315541 1105 80-81 9.326905183637038 1105 82-83 9.27930571368255 1105 84-85 9.094240725215816 1105 86-87 8.93573285709158 1105 88-89 8.722844581392415 1105 90-91 8.40076225631628 1105 92-93 8.350774930910958 1105 94-95 8.161532913228015 1105 96-97 7.931738275994281 1105 98-99 7.289484955242523 1105 100-101 6.770769855659115 1106 1 8.437275216835538 1106 2 9.330757759422923 1106 3 8.945326063289954 1106 4 10.780316582779125 1106 5 10.931221707782488 1106 6 10.133631361982665 1106 7 9.506909426168715 1106 8 9.127724001899328 1106 9 7.868982816542012 1106 10-11 7.188099872046703 1106 12-13 7.004975919681339 1106 14-15 8.069906954401155 1106 16-17 9.571523112225155 1106 18-19 11.101121561981667 1106 20-21 11.40559230484737 1106 22-23 11.723151079242768 1106 24-25 11.45141177963595 1106 26-27 11.799860060732172 1106 28-29 11.77479659284407 1106 30-31 11.414560812677184 1106 32-33 11.707211766829097 1106 34-35 11.695499237618943 1106 36-37 11.765193661726283 1106 38-39 11.4092000485999 1106 40-41 11.393081117376884 1106 42-43 11.08498032585926 1106 44-45 10.753755835456065 1106 46-47 10.606051448719018 1106 48-49 10.678614435129639 1106 50-51 12.904116411591815 1106 52-53 11.692683468132568 1106 54-55 11.075638287570815 1106 56-57 10.851406719729582 1106 58-59 10.741104798211328 1106 60-61 10.706983787824786 1106 62-63 10.71323340496766 1106 64-65 10.497757578736529 1106 66-67 10.200003373548412 1106 68-69 9.987663952012326 1106 70-71 9.892371876864054 1106 72-73 9.633172518935016 1106 74-75 9.472975457684186 1106 76-77 9.137514928021123 1106 78-79 8.998198102800643 1106 80-81 8.716705627096015 1106 82-83 8.651589527429781 1106 84-85 8.44878617976127 1106 86-87 8.310899154209093 1106 88-89 8.00688005811082 1106 90-91 7.841338752990339 1106 92-93 7.610641893216947 1106 94-95 7.451998545157064 1106 96-97 6.984953353599604 1106 98-99 6.663986063889974 1106 100-101 6.0142288357034595 1107 1 7.782039590215508 1107 2 8.441241894834057 1107 3 8.437604372836812 1107 4 10.078331947695897 1107 5 10.437205476246934 1107 6 9.87417838581078 1107 7 9.224722736184017 1107 8 7.985649855245569 1107 9 4.698664900174631 1107 10-11 6.384490814709327 1107 12-13 6.65229232922508 1107 14-15 7.741612436740866 1107 16-17 9.061632044644504 1107 18-19 10.636381235321043 1107 20-21 11.176231592346202 1107 22-23 10.85530273362582 1107 24-25 11.295942712461272 1107 26-27 11.293594641163764 1107 28-29 11.263928041736982 1107 30-31 11.13534648848368 1107 32-33 11.326325200200948 1107 34-35 11.443119158920592 1107 36-37 11.68327847325622 1107 38-39 11.280843755073775 1107 40-41 10.984383895241404 1107 42-43 10.831382296441067 1107 44-45 10.193550130606116 1107 46-47 10.440472317055427 1107 48-49 10.727502859913375 1107 50-51 12.63034773244376 1107 52-53 11.061559752445987 1107 54-55 10.710369303935071 1107 56-57 10.463090263341584 1107 58-59 10.55865021762359 1107 60-61 10.188637917146163 1107 62-63 10.41998073484493 1107 64-65 10.326687128875498 1107 66-67 10.334991838149769 1107 68-69 10.049187737184557 1107 70-71 9.698967743638981 1107 72-73 9.52688709296456 1107 74-75 9.445423323928217 1107 76-77 9.59873334718355 1107 78-79 9.276242686823629 1107 80-81 8.907696500272994 1107 82-83 8.7593357135264 1107 84-85 8.504402806350072 1107 86-87 8.130845341867193 1107 88-89 8.050978657467493 1107 90-91 8.005741313029063 1107 92-93 7.600763317438549 1107 94-95 7.473292773944422 1107 96-97 6.973327779757437 1107 98-99 6.63769706604481 1107 100-101 6.054932787797398 1108 1 7.048514985798885 1108 2 8.397005799248198 1108 3 8.354495379369837 1108 4 9.373951343080549 1108 5 10.319299739356456 1108 6 9.306543409284956 1108 7 8.402809776947112 1108 8 5.6386612880398665 1108 9 5.066526390101846 1108 10-11 4.575422283149603 1108 12-13 4.149250156435254 1108 14-15 7.010058762845645 1108 16-17 8.825163464645996 1108 18-19 8.97172353603893 1108 20-21 9.529090644809127 1108 22-23 10.595965459983972 1108 24-25 10.265398335085909 1108 26-27 8.615529335751038 1108 28-29 10.462678802474244 1108 30-31 10.664797500108993 1108 32-33 11.22924813906873 1108 34-35 10.842498459414909 1108 36-37 10.59570131276239 1108 38-39 10.256055752366166 1108 40-41 10.537966967750531 1108 42-43 9.793306620161164 1108 44-45 9.289554785076149 1108 46-47 10.104507065670571 1108 48-49 10.733053913146353 1108 50-51 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30-31 -20.813377103065616 2313 32-33 -20.75598113782722 2313 34-35 -20.504944221361104 2313 36-37 -20.03547152674378 2313 38-39 -20.03935870971496 2313 40-41 -20.022218217434652 2313 42-43 -19.95470925285471 2313 44-45 -19.616088953900924 2313 46-47 -19.31723014773331 2313 48-49 -19.169944323185216 2313 50-51 -16.566615295725256 2313 52-53 -18.713968416568097 2313 54-55 -19.594428231276193 2313 56-57 -19.634180863418976 2313 58-59 -19.408119148573594 2313 60-61 -19.15665257581158 2313 62-63 -18.773241096140985 2313 64-65 -18.482951955631542 2313 66-67 -18.170950063258683 2313 68-69 -17.760451347322487 2313 70-71 -17.45552169298074 2313 72-73 -17.1009738225284 2313 74-75 -16.70928618311404 2313 76-77 -16.339646934506593 2313 78-79 -16.01843160895102 2313 80-81 -15.640278851839682 2313 82-83 -15.213155483656696 2313 84-85 -14.8829211373119 2313 86-87 -14.429233883484919 2313 88-89 -14.070060074926875 2313 90-91 -13.65733087006953 2313 92-93 -13.150777175519195 2313 94-95 -12.613855113379522 2313 96-97 -12.009946868129887 2313 98-99 -11.32004941282843 2313 100-101 -10.19286650797725 2314 1 -18.30950970555914 2314 2 -17.726891907982843 2314 3 -18.43959633138854 2314 4 -20.037865235402695 2314 5 -19.210241706851658 2314 6 -19.776789924048376 2314 7 -19.50062937649204 2314 8 -19.271832539120627 2314 9 -18.128799888557765 2314 10-11 -18.123651790924473 2314 12-13 -17.747795698943936 2314 14-15 -19.217454464667583 2314 16-17 -20.485683096200564 2314 18-19 -20.627703271721213 2314 20-21 -20.89485115413267 2314 22-23 -20.850463111444597 2314 24-25 -20.885395315707736 2314 26-27 -20.743377189822155 2314 28-29 -20.677753296291186 2314 30-31 -20.813377103065616 2314 32-33 -20.75598113782722 2314 34-35 -20.504944221361104 2314 36-37 -20.03547152674378 2314 38-39 -20.03935870971496 2314 40-41 -20.022218217434652 2314 42-43 -19.95470925285471 2314 44-45 -19.616088953900924 2314 46-47 -19.31723014773331 2314 48-49 -19.169944323185216 2314 50-51 -16.566615295725256 2314 52-53 -18.713968416568097 2314 54-55 -19.594428231276193 2314 56-57 -19.634180863418976 2314 58-59 -19.408119148573594 2314 60-61 -19.15665257581158 2314 62-63 -18.773241096140985 2314 64-65 -18.482951955631542 2314 66-67 -18.170950063258683 2314 68-69 -17.760451347322487 2314 70-71 -17.45552169298074 2314 72-73 -17.1009738225284 2314 74-75 -16.70928618311404 2314 76-77 -16.339646934506593 2314 78-79 -16.01843160895102 2314 80-81 -15.640278851839682 2314 82-83 -15.213155483656696 2314 84-85 -14.8829211373119 2314 86-87 -14.429233883484919 2314 88-89 -14.070060074926875 2314 90-91 -13.65733087006953 2314 92-93 -13.150777175519195 2314 94-95 -12.613855113379522 2314 96-97 -12.009946868129887 2314 98-99 -11.32004941282843 2314 100-101 -10.19286650797725 2315 1 -18.30950970555914 2315 2 -17.726891907982843 2315 3 -18.43959633138854 2315 4 -20.037865235402695 2315 5 -19.210241706851658 2315 6 -19.776789924048376 2315 7 -19.50062937649204 2315 8 -19.271832539120627 2315 9 -18.128799888557765 2315 10-11 -18.123651790924473 2315 12-13 -17.747795698943936 2315 14-15 -19.217454464667583 2315 16-17 -20.485683096200564 2315 18-19 -20.627703271721213 2315 20-21 -20.89485115413267 2315 22-23 -20.850463111444597 2315 24-25 -20.885395315707736 2315 26-27 -20.743377189822155 2315 28-29 -20.677753296291186 2315 30-31 -20.813377103065616 2315 32-33 -20.75598113782722 2315 34-35 -20.504944221361104 2315 36-37 -20.03547152674378 2315 38-39 -20.03935870971496 2315 40-41 -20.022218217434652 2315 42-43 -19.95470925285471 2315 44-45 -19.616088953900924 2315 46-47 -19.31723014773331 2315 48-49 -19.169944323185216 2315 50-51 -16.566615295725256 2315 52-53 -18.713968416568097 2315 54-55 -19.594428231276193 2315 56-57 -19.634180863418976 2315 58-59 -19.408119148573594 2315 60-61 -19.15665257581158 2315 62-63 -18.773241096140985 2315 64-65 -18.482951955631542 2315 66-67 -18.170950063258683 2315 68-69 -17.760451347322487 2315 70-71 -17.45552169298074 2315 72-73 -17.1009738225284 2315 74-75 -16.70928618311404 2315 76-77 -16.339646934506593 2315 78-79 -16.01843160895102 2315 80-81 -15.640278851839682 2315 82-83 -15.213155483656696 2315 84-85 -14.8829211373119 2315 86-87 -14.429233883484919 2315 88-89 -14.070060074926875 2315 90-91 -13.65733087006953 2315 92-93 -13.150777175519195 2315 94-95 -12.613855113379522 2315 96-97 -12.009946868129887 2315 98-99 -11.32004941282843 2315 100-101 -10.19286650797725 2316 1 -18.30950970555914 2316 2 -17.726891907982843 2316 3 -18.43959633138854 2316 4 -20.037865235402695 2316 5 -19.210241706851658 2316 6 -19.776789924048376 2316 7 -19.50062937649204 2316 8 -19.271832539120627 2316 9 -18.128799888557765 2316 10-11 -18.123651790924473 2316 12-13 -17.747795698943936 2316 14-15 -19.217454464667583 2316 16-17 -20.485683096200564 2316 18-19 -20.627703271721213 2316 20-21 -20.89485115413267 2316 22-23 -20.850463111444597 2316 24-25 -20.885395315707736 2316 26-27 -20.743377189822155 2316 28-29 -20.677753296291186 2316 30-31 -20.813377103065616 2316 32-33 -20.75598113782722 2316 34-35 -20.504944221361104 2316 36-37 -20.03547152674378 2316 38-39 -20.03935870971496 2316 40-41 -20.022218217434652 2316 42-43 -19.95470925285471 2316 44-45 -19.616088953900924 2316 46-47 -19.31723014773331 2316 48-49 -19.169944323185216 2316 50-51 -16.566615295725256 2316 52-53 -18.713968416568097 2316 54-55 -19.594428231276193 2316 56-57 -19.634180863418976 2316 58-59 -19.408119148573594 2316 60-61 -19.15665257581158 2316 62-63 -18.773241096140985 2316 64-65 -18.482951955631542 2316 66-67 -18.170950063258683 2316 68-69 -17.760451347322487 2316 70-71 -17.45552169298074 2316 72-73 -17.1009738225284 2316 74-75 -16.70928618311404 2316 76-77 -16.339646934506593 2316 78-79 -16.01843160895102 2316 80-81 -15.640278851839682 2316 82-83 -15.213155483656696 2316 84-85 -14.8829211373119 2316 86-87 -14.429233883484919 2316 88-89 -14.070060074926875 2316 90-91 -13.65733087006953 2316 92-93 -13.150777175519195 2316 94-95 -12.613855113379522 2316 96-97 -12.009946868129887 2316 98-99 -11.32004941282843 2316 100-101 -10.19286650797725 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 600054.0 3 10817.0 4 26057.0 5 15227.0 6 14346.0 7 14204.0 8 12139.0 9 13037.0 10 14939.0 11 16305.0 12 18727.0 13 19622.0 14 20707.0 15 22015.0 16 23501.0 17 23313.0 18 22553.0 19 24003.0 20 25609.0 21 27958.0 22 30527.0 23 33141.0 24 36813.0 25 41007.0 26 45398.0 27 51110.0 28 57120.0 29 64266.0 30 72986.0 31 83438.0 32 95820.0 33 110084.0 34 124259.0 35 131688.0 36 113662.0 37 56412.0 38 8089.0 39 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.7726957412069 20.942211397777154 15.0861664197845 23.198926441231443 2 14.08634604334414 20.539760980686296 42.38760972030519 22.986283255664375 3 18.00446456450904 26.35868600250045 30.674839864667124 24.96200956832339 4 11.36712432148462 17.16838915015407 41.37953793619322 30.084948592168086 5 11.89934749090483 38.2913424867312 37.04113589720524 12.768174125158735 6 28.455950085824593 39.91561000901032 18.536602554099698 13.091837351065383 7 24.584825123450475 35.1117641406204 23.25035105502497 17.053059680904155 8 22.982044421824728 39.5648885448807 20.522562578270698 16.930504455023872 9 23.951258019728694 20.525478081725822 21.116568722041926 34.40669517650356 10-11 22.354280290344953 31.020292413983874 28.22436567558516 18.40106162008601 12-13 23.028793390192554 28.21036403468731 28.690872735688945 20.069969839431195 14-15 20.719643490578672 28.678030365318797 26.80970933769965 23.792616806402886 16-17 20.332182528045138 31.090263011762946 27.79433544208862 20.783219018103296 18-19 20.235446810680266 29.45650581469269 30.06533986215638 20.24270751247066 20-21 21.007676975913064 28.55454478435196 30.262321582869035 20.175456656865943 22-23 20.68510376853623 28.324355125666845 30.027854068057426 20.962687037739506 24-25 20.580978368996803 28.431869537860322 30.324456960401662 20.662695132741216 26-27 20.587035609436715 28.55827058736562 30.09865989397428 20.756033909223394 28-29 20.50268206220713 28.607692641338172 30.17938141131275 20.71024388514195 30-31 20.787009377354078 28.385848247915423 30.14877782684365 20.678364547886847 32-33 20.666218228991497 28.511795512420125 29.960953890997615 20.861032367590767 34-35 20.813899301045918 28.54815254572167 30.018724715469535 20.619223437762873 36-37 20.871327682193385 28.285533196521733 30.030222217601914 20.812916903682964 38-39 20.74069952677693 28.53436854133804 30.027311843071107 20.697620088813924 40-41 20.96124004831276 28.435866626520678 29.98708387228698 20.61580945287958 42-43 20.83694333737654 28.459627612009204 29.79752146081528 20.905907589798982 44-45 20.811074431404958 28.744431582190888 29.640508822873706 20.80398516353044 46-47 20.92411627754353 28.64631153373061 29.408833033458027 21.020739155267833 48-49 20.938326110997536 28.73551522129805 29.329497718401935 20.996660949302473 50-51 21.195538267058076 28.90075385992363 28.917560493996408 20.986147379021894 52-53 20.805984834717652 29.036100169359862 29.04532258654634 21.112592409376145 54-55 20.74623587436738 28.956204787085554 29.17673613603554 21.120823202511524 56-57 20.755489742142334 29.108751686924485 29.186436196311472 20.949322374621715 58-59 20.728402848054387 28.970283699070336 29.272065952048624 21.02924750082665 60-61 20.782556863485702 29.101381894446238 29.227833203548094 20.88822803851997 62-63 20.68903643118437 28.937094256965736 29.234974879106083 21.13889443274381 64-65 20.561811589245398 28.902997271061853 29.266257706019626 21.26893343367312 66-67 20.570959678581914 28.98275043606729 29.006470537644415 21.439819347706386 68-69 20.625234246614834 29.009038780544287 29.06772249824615 21.298004474594727 70-71 20.710516580432632 28.784101607111428 29.100635895590194 21.404745916865746 72-73 20.7029929661866 28.899773468851514 28.994196846040744 21.403036718921147 74-75 20.646750265265915 29.07851108544642 28.79925602369181 21.47548262559586 76-77 20.660961300068152 28.968155679456427 28.90383529879562 21.467047721679798 78-79 20.52926187704443 29.028662510622016 28.829181911810366 21.612893700523188 80-81 20.518566860314856 29.066967169482922 28.7471892094662 21.66727676073602 82-83 20.683716177232768 28.93521234879377 28.900741729496094 21.480329744477366 84-85 20.794212681882733 28.94188278946146 28.721962348914953 21.541942179740857 86-87 20.53948279026031 29.031787973032458 28.810556730311376 21.618172506395855 88-89 20.654545175216235 28.879952681630332 28.803550768568975 21.661951374584458 90-91 20.649436049662157 28.892414244541527 28.839813647022716 21.6183360587736 92-93 20.614986801379487 28.774520819662797 28.75043071548209 21.860061663475626 94-95 20.47750585756429 28.831800481870346 28.79789722843124 21.892796432134126 96-97 20.485320918713054 28.736506694081637 28.761682515946635 22.016489871258674 98-99 20.290839492386198 28.684371805598317 28.612707043614037 22.412081658401448 100-101 20.48672973703381 28.764870825727286 28.578181670718454 22.17021776652045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 129018.0 1 117319.0 2 78109.0 3 39529.5 4 24256.0 5 18791.0 6 17677.5 7 18536.5 8 19245.5 9 18576.5 10 16559.0 11 13583.0 12 10595.5 13 7984.5 14 6171.0 15 5334.5 16 5203.0 17 5790.0 18 6706.5 19 7773.0 20 8887.5 21 10107.5 22 11588.0 23 13109.5 24 14955.0 25 17117.5 26 19343.0 27 21931.0 28 24870.0 29 28450.5 30 32284.0 31 36041.5 32 40477.5 33 44781.0 34 48627.0 35 52363.0 36 56562.5 37 60757.5 38 63008.5 39 63820.0 40 64218.5 41 63748.0 42 63280.5 43 63498.5 44 63386.0 45 63231.5 46 62154.5 47 60295.0 48 58463.0 49 55634.0 50 51754.0 51 47090.0 52 42368.5 53 38108.5 54 33497.5 55 28421.0 56 23220.0 57 18286.5 58 14122.0 59 10803.5 60 8032.5 61 5688.0 62 3910.0 63 2542.5 64 1605.0 65 950.5 66 562.0 67 335.0 68 187.5 69 104.5 70 63.0 71 43.0 72 28.5 73 17.0 74 11.0 75 10.5 76 7.5 77 3.5 78 1.0 79 0.5 80 0.5 81 1.5 82 1.5 83 0.0 84 0.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.17973222342814 2 29.19040915181455 3 22.99438666831367 4 29.28097869800502 5 32.390941725262635 6 25.069706962021137 7 23.95199106845662 8 19.961509121546122 9 11.846081747384044 10-11 15.46016495821354 12-13 15.563605658027122 14-15 19.721153293082295 16-17 22.992359343750323 18-19 26.948565361415394 20-21 29.012004590239986 22-23 28.595719422052202 24-25 27.13029379233219 26-27 28.56726615754103 28-29 25.541154217165126 30-31 27.399150032390047 32-33 26.40458231927816 34-35 24.475394408921737 36-37 27.435948330568944 38-39 27.549266344243485 40-41 24.604105945152853 42-43 25.448486625787005 44-45 24.184449760539966 46-47 27.441912436551814 48-49 22.85589211238733 50-51 28.465711341042194 52-53 22.038550283778292 54-55 23.15369666132647 56-57 25.290048853807267 58-59 24.57272956150384 60-61 24.106845662138348 62-63 23.9329907940606 64-65 22.47074759243421 66-67 22.97885170450849 68-69 23.957247968196462 70-71 22.450332905552134 72-73 22.30646358217878 74-75 19.61926844700338 76-77 21.861795870104128 78-79 20.964822618530125 80-81 22.013232300277885 82-83 22.859074540480957 84-85 19.820963667515 86-87 21.382899375866916 88-89 20.2098799540976 90-91 21.347727652046984 92-93 21.32401266936411 94-95 20.386092176318773 96-97 19.286080012371396 98-99 18.685567617874778 100-101 20.36049131032116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2121022.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.848614929508 #Duplication Level Percentage of deduplicated Percentage of total 1 87.53087972081187 66.3909599038494 2 8.431716982803556 12.790681092465196 3 2.012726788894904 4.57987617507584 4 0.7630151521755211 2.3149456985096415 5 0.38915796206120284 1.4758546205566128 6 0.2326878228709702 1.058942944543547 7 0.1407137132985602 0.7471058178698576 8 0.09941881452237315 0.6032623503564526 9 0.06681620431517542 0.4561124896937763 >10 0.3186244394480446 3.9498052166499598 >50 0.010975253180010676 0.5647103356431222 >100 0.003016093868672073 0.3675291939333698 >500 6.27629372749997E-5 0.04370122348596577 >1k 1.255258745499994E-4 0.16752135669620213 >5k 0.0 0.0 >10k+ 6.27629372749997E-5 4.488991580671149 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 94297 4.445828473254874 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.7147082868541674E-5 0.0 5 4.7147082868541674E-5 0.0 0.0 4.7147082868541674E-5 0.0 6 4.7147082868541674E-5 0.0 0.0 4.7147082868541674E-5 4.7147082868541674E-5 7 4.7147082868541674E-5 0.0 0.0 4.7147082868541674E-5 4.7147082868541674E-5 8 4.7147082868541674E-5 0.0 0.0 4.7147082868541674E-5 4.7147082868541674E-5 9 4.7147082868541674E-5 0.0 0.0 9.429416573708335E-5 4.7147082868541674E-5 10-11 4.7147082868541674E-5 0.0 0.0 1.4144124860562504E-4 4.7147082868541674E-5 12-13 4.7147082868541674E-5 0.0 0.0 1.4144124860562504E-4 4.7147082868541674E-5 14-15 4.7147082868541674E-5 0.0 0.0 1.4144124860562504E-4 4.7147082868541674E-5 16-17 4.7147082868541674E-5 0.0 0.0 1.4144124860562504E-4 4.7147082868541674E-5 18-19 4.7147082868541674E-5 0.0 0.0 1.6501479003989587E-4 4.7147082868541674E-5 20-21 4.7147082868541674E-5 0.0 0.0 1.885883314741667E-4 4.7147082868541674E-5 22-23 4.7147082868541674E-5 0.0 0.0 1.885883314741667E-4 4.7147082868541674E-5 24-25 4.7147082868541674E-5 0.0 0.0 3.064560386455209E-4 4.7147082868541674E-5 26-27 4.7147082868541674E-5 0.0 0.0 3.3002958007979173E-4 4.7147082868541674E-5 28-29 4.7147082868541674E-5 0.0 0.0 5.186179115539585E-4 4.7147082868541674E-5 30-31 4.7147082868541674E-5 0.0 0.0 0.0017208685247017711 4.7147082868541674E-5 32-33 4.7147082868541674E-5 0.0 0.0 0.004290384541037293 4.7147082868541674E-5 34-35 9.429416573708335E-5 0.0 0.0 0.007755695131875105 4.7147082868541674E-5 36-37 1.4144124860562504E-4 0.0 0.0 0.01669006733546375 4.7147082868541674E-5 38-39 1.4144124860562504E-4 0.0 0.0 0.027887499516742402 4.7147082868541674E-5 40-41 1.4144124860562504E-4 0.0 0.0 0.03941496127810084 4.7147082868541674E-5 42-43 1.4144124860562504E-4 0.0 0.0 0.05200323240400147 4.7147082868541674E-5 44-45 1.4144124860562504E-4 0.0 0.0 0.06673669580042074 4.7147082868541674E-5 46-47 1.4144124860562504E-4 0.0 0.0 0.08564267603070595 4.7147082868541674E-5 48-49 1.4144124860562504E-4 0.0 0.0 0.10794324622752616 4.7147082868541674E-5 50-51 1.4144124860562504E-4 0.0 0.0 0.13488780408689774 4.7147082868541674E-5 52-53 1.4144124860562504E-4 0.0 2.3573541434270837E-5 0.16529767253710712 4.7147082868541674E-5 54-55 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.19832420408652057 4.7147082868541674E-5 56-57 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.23528751705545722 4.7147082868541674E-5 58-59 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.2769183912283795 4.7147082868541674E-5 60-61 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.3247726803399493 4.7147082868541674E-5 62-63 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.37253267528578204 4.7147082868541674E-5 64-65 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.42399371623679527 4.7147082868541674E-5 66-67 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.4864164539547444 4.7147082868541674E-5 68-69 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.5607438300970005 4.7147082868541674E-5 70-71 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.6441941667743192 4.7147082868541674E-5 72-73 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.730779784462396 4.7147082868541674E-5 74-75 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.8179547406863295 4.7147082868541674E-5 76-77 1.4144124860562504E-4 0.0 4.7147082868541674E-5 0.9148891430640512 4.7147082868541674E-5 78-79 1.4144124860562504E-4 0.0 4.7147082868541674E-5 1.028796495274448 4.7147082868541674E-5 80-81 1.4144124860562504E-4 0.0 7.072062430281252E-5 1.1585452673286745 4.7147082868541674E-5 82-83 1.4144124860562504E-4 0.0 9.429416573708335E-5 1.293385924332704 4.7147082868541674E-5 84-85 1.4144124860562504E-4 0.0 9.429416573708335E-5 1.4311025533917139 4.7147082868541674E-5 86-87 1.4144124860562504E-4 0.0 9.429416573708335E-5 1.5813131594108878 4.7147082868541674E-5 88-89 1.4144124860562504E-4 0.0 9.429416573708335E-5 1.7524806437651284 4.7147082868541674E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1710 0.0 52.74039 1 GTATCAA 3250 0.0 41.939674 1 ATCAACG 3630 0.0 35.241653 3 TCAACGC 3700 0.0 34.164135 4 CAACGCA 3715 0.0 34.020176 5 TATCAAC 3900 0.0 33.893734 2 AACGCAG 3955 0.0 31.140663 6 ACGCAGA 4655 0.0 26.284494 7 GTACATG 3140 0.0 25.784233 1 GTGGTAT 1045 0.0 25.498455 1 CGCAGAG 4710 0.0 24.798605 8 TGGTATC 1010 0.0 24.349169 2 TACATGG 3260 0.0 24.045738 2 GCAGAGT 5170 0.0 21.253815 9 ACATGGG 3345 0.0 20.7784 3 GAGTACA 2450 0.0 19.660229 1 GAGTACT 3520 0.0 19.51368 12-13 GTGTAGC 805 0.0 19.09643 1 CAGAGTA 5005 0.0 18.591505 10-11 AGAGTAC 4750 0.0 18.28682 10-11 >>END_MODULE