FastQCFastQC Report
Sat 18 Jun 2016
SRR1930990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121022
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55790.263033575323594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49360.2327180010391217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.13898960029646087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.058.7206651
GTATCAA58850.042.6324161
ATCAACG75650.033.09733
CAACGCA76450.032.7666975
TCAACGC76850.032.5804984
TATCAAC77900.032.0223922
AACGCAG81250.031.1221686
ACGCAGA94150.026.656397
CGCAGAG94400.026.4852818
GTGGTAT15250.024.303971
GCAGAGT104900.023.2915139
TGGTATC14800.021.5102312
GTACATG57800.021.210181
AGAGTAC98150.021.09890610-11
GAGTACT74100.020.90407612-13
TACATGG57600.020.3754142
ACATGGG56600.020.1458573
CAGAGTA102650.019.87355210-11
AGTACTT80850.019.59893212-13
GTACTTT79500.019.15595814-15
Produced by FastQC (version 0.11.5)