FastQCFastQC Report
Sat 18 Jun 2016
SRR1930989_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930989_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977878
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN886254.48081226445716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.052.8450131
GTATCAA28300.045.605971
ATCAACG31400.039.9327473
CAACGCA31900.038.036755
TCAACGC31950.037.981554
TATCAAC35800.035.476662
AACGCAG35100.034.9078526
ACGCAGA41800.028.690827
CGCAGAG41750.028.042468
GCAGAGT46400.023.9316129
GTACATG30800.023.4463921
TACATGG31150.022.5235372
AGAGTAC42550.020.39406810-11
GAGTACT30950.020.25127612-13
ACATGGG31800.020.111113
CAGAGTA46100.019.12159210-11
TCAACGA2652.1712185E-719.0011834
GCGTAGA3555.802576E-1018.755241
ATAGGAC4600.018.6101783
GTACTTT34900.018.15396514-15
Produced by FastQC (version 0.11.5)