FastQCFastQC Report
Sat 18 Jun 2016
SRR1930989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1977878
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44540.22519083583517285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40330.20390539760288554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23460.11861196696661776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.057.4434241
GTATCAA49500.039.649151
CAACGCA61400.031.521045
ATCAACG62250.031.2197193
TCAACGC62450.031.1189464
TATCAAC64450.030.1555582
AACGCAG64650.029.9357016
ACGCAGA73950.026.0426797
CGCAGAG73800.025.838518
GCAGAGT85000.021.9874619
GGCGTGC5100.021.39228
GTACATG47200.020.7402631
GAGTACT61600.020.44474212-13
AGAGTAC77700.020.35975810-11
GTGGTAT14100.019.884831
CAGAGTA83250.019.82863410-11
GGACCGT1200.004802652619.7645326
TACATGG47400.019.5489432
TGGTATC13100.019.2252312
ACATGGG47150.019.0469363
Produced by FastQC (version 0.11.5)