FastQCFastQC Report
Sat 18 Jun 2016
SRR1930988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935267
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN834274.3108780338836965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.058.563191
GTATCAA31800.043.3729131
ATCAACG34300.037.6377753
TCAACGC35100.037.350964
CAACGCA35650.036.4845735
TATCAAC39050.034.271142
AACGCAG37750.034.1104976
ACGCAGA45850.028.509487
CGCAGAG44850.027.510518
GTACATG34050.025.504321
GTGGTAT9250.025.4037631
GCAGAGT49000.023.5038839
TACATGG36250.023.2500672
GAGTACA26850.022.6404291
ACATGGG35300.022.3413753
GAGTACT31500.021.78879712-13
TATACCG1403.3323624E-421.522485
TGGTATC10050.020.8384822
AGAGTAC45050.020.22535510-11
AGTACAT27100.020.1679972
Produced by FastQC (version 0.11.5)